Labshake search
Citations for Quantifoil Micro Tools :
301 - 350 of 784 citations for 1 3 Bis 2 4 hydroxyphenyl 2 propyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 4 μl of KMN complex at 0.3 mg/ml supplemented with 0.003% Triton were applied to glow-discharged UltrAuFoil R2/2 grids (Quantifoil). Excess liquid was removed by blotting (3.5 s at blot force −3) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then seeded onto gold grids (either ‘UltrAuFoil’ 200 Au mesh, R2/2 gold film, or Quantifoil 300 Au mesh ...
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bioRxiv - Microbiology 2022Quote: Phage particles were negatively stained for 20 s with 2 % uranyl acetate on carbon-coated copper grids (Quantifoil) and observed at 100 kV on a Hitachi HT 7700 equipped with an AMT XR81B Peltier cooled CCD camera (8M pixel ...
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bioRxiv - Microbiology 2023Quote: ... Phage particles were negatively stained for 20 seconds with 2% uranyl acetate on carbon-coated copper grids (Quantifoil) and observed at 100 kV on a Hitachi HT 7700 equipped with an AMT XR81B Peltier cooled CCD camera (8M pixel ...
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bioRxiv - Microbiology 2022Quote: ... the amoebae (5×105 per well) were seeded onto EM gold finder grids (Au NH2 R2/2, Quantifoil) and incubated for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... Differentiated U937 cells became adhesive and were seeded onto gold London Finder grids (Au H2 R2/2, Quantifoil) coated with vitronectin (25 µg/mL in 1xPBS ...
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bioRxiv - Biochemistry 2024Quote: About 3 µl of the purified complex sample was applied to a freshly-glow discharged Au 300 mesh R1.2/1.3 grids coated with 2 nm carbon film (Quantifoil). The grids were blotted with force level of 1 and blot time of 4s ...
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bioRxiv - Microbiology 2024Quote: Cryo-ET samples were prepared using copper grids with holey carbon support film (300 mesh, R2/2, Quantifoil) as previously described116 ...
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bioRxiv - Biophysics 2024Quote: ... Regions of the LCP drops were scooped and gently smeared across pre-clipped Cu200 R2:2 grids (Quantifoil) under a humidifier ...
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bioRxiv - Microbiology 2021Quote: ... 4-7 µl of cells were deposited on R2/1 Cu 200 grids (Quantifoil) that had been glow-discharged for 1 min at 0.19 mbar and 20 mA in a PELCO easiGlow device shortly before use ...
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bioRxiv - Cell Biology 2020Quote: ... HeLa cells were grown for 24 h on holey carbon-covered gold EM grids (200 mesh, R2/2, Quantifoil), fed with oleic acid and induced with doxycycline for Fsp27-EGFP expression for an unrelated project ...
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bioRxiv - Biophysics 2020Quote: Cryo-EM grids were prepared by applying freshly purified channel protein (~3.5 μl) to glow-discharged copper Quantifoil R2/2 holey carbon grids (Quantifoil), which were then blotted for 2 s with ~100% humidity and flash frozen in liquid ethane using FEI Vitrobot Mark IV (FEI) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were grown on 200 mesh gold R2/2 London Finder Quantifoil grids (Quantifoil Micro Tools GmbH, Jena, Germany). Prior to seeding cells ...
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bioRxiv - Biophysics 2023Quote: ... 4 μl of 4.3 mg/ml Slo1-γ1 complex were applied to glow-discharged UltrAuFoil R2/2 200 grid (Quantifoil). Any excess liquid was removed by blotting for 3.5 s at a blot force of −3 ...
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bioRxiv - Biophysics 2024Quote: ... This bleb/beads suspension was then applied onto glow discharged plasma-cleaned carbon-coated copper grids (GR1/2, Quantifoil) and manually blotted for ∼3 seconds with 55 mm Whatman filter paper (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... This mixture was applied to the front (3.7 µL) and to the back (1.3 µL) of carbon-coated copper grids (R2/2, Cu 200 mesh; Quantifoil) previously glow-discharged (2 mA ...
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bioRxiv - Microbiology 2024Quote: ... 3.5 µL of sample was applied to a glow discharged UltrAufoil holey gold grids (R2/2, 200 mesh, Quantifoil). Grid were blotted for 5 s at 4°C and 95% relative humidity before freezing in liquid ethane using a Vitrobot Mark IV (FEI) ...
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bioRxiv - Biochemistry 2024Quote: ... 3 µL of the samples were applied to a holey carbon R3.5/1 Cu200 grid (Quantifoil) covered with a 2 nm continuous carbon foil ...
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bioRxiv - Microbiology 2020Quote: ... 3.5 μl of the freshly prepared mixture of the soluble S trimer and monomeric ACE2 (1:3 molar ratio) at ~1 mg/ml was applied to a 1.2/1.3 Quantifoil grid (Quantifoil Micro Tools GmbH, Germany), which had been glow discharged with a PELCO easiGlow™ Glow Discharge Cleaning system (Ted Pella ...
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bioRxiv - Biophysics 2021Quote: ... 4 μl of cell suspension was applied to holey carbon R2/1 Cu grids (Quantifoil) and plunge-frozen on a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: Quantifoil R2/1 or SiO2 R1/4 gold grids (both Quantifoil Mirco Tools GmbH, Germany) were briefly washed in chloroform and placed on drops of poly-D-lysine (100 µg/ml) ...
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bioRxiv - Biophysics 2020Quote: ... 3.5 µl of purified channel proteins at ∼6 mg/ml were pipetted onto glow-discharged copper Quantifoil R2/2 holey carbon grids (Quantifoil). Grids were blotted for 2 s at ∼100% humidity and flash frozen in liquid ethane ...
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bioRxiv - Biochemistry 2022Quote: ... 3.0 μL of sample was applied to glow-discharged 200-mesh copper 2 nm C R1.2/1.3 cryo-EM grids (Quantifoil Micro Tools), blotted 1 s at 100 % humidity ...
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bioRxiv - Biochemistry 2022Quote: ... 2.5 μL of sample was applied to glow-discharged 200-mesh copper 2 nm C R1.2/1.3 cryo-EM grids (Quantifoil Micro Tools), blotted 3 s at 75 % humidity ...
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bioRxiv - Cell Biology 2022Quote: Cells were applied onto galectin-8 coated EM grids with carbon support film (R2/2, Au mesh; Quantifoil, Jena, Germany). After 15-20 min incubation ...
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bioRxiv - Cell Biology 2021Quote: HeLa Doxycycline-inducible stable cell lines were grown for approximately 48 h on holey carbon gold EM grids (200 mesh, R2/2, Quantifoil) prior to plunge freezing ...
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bioRxiv - Microbiology 2020Quote: ... 3,5 μl of the culture was mixed with 10 nm gold fiducial markers (Cell Microscopy Core, UMC Utrecht) and applied onto glow-discharged R2/2 200 mesh holey carbon EM grids (Quantifoil). The grid was then rapidly plunged in liquid ethane using the Leica GP automated freeze-plunger ...
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bioRxiv - Evolutionary Biology 2020Quote: ... was applied onto a glow-discharged (20 mA for 30 seconds) holey-carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ~3 nm thickness ...
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bioRxiv - Biochemistry 2021Quote: ... 3.5 μL sample was applied onto a freshly glow-discharged 200-mesh copper 2 nm C R1.2/1.3 grids (Quantifoil Micro Tools). The grids were blotted for 1 s and plunge-vitrified in liquid ethane ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples for cryo-ET were deposited onto 300-mesh holey carbon grids (Quantifoil Micro Tools, R2/2 X-103-Cu300) and samples for cryo-SPA were deposited onto 200-mesh holey carbon grids (Quantifoil Micro Tools ...
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bioRxiv - Cell Biology 2024Quote: ... 4 × 104 MDM cells were seeded on glow discharged and UV-light sterilized holey carbon grids (R2/2, Au, 200 mesh, Quantifoil), which were placed in a glass-bottomed ‘microwell’ of 35-mm MatTek dish (MatTek ...
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bioRxiv - Biophysics 2023Quote: A 3 µL aliquot of purified CDTbD623A/D734Aprotein sample at a concentration of 30 µM was applied to a glow discharged QUANTIFOIL® copper R 1.2/1.3 grid with a 2 nm thin extra carbon layer (QUANTIFOIL, GMBH). For calcium depleted CDTb (28 µM) ...
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bioRxiv - Biophysics 2023Quote: ... 3 or 3.5 μl of LRRK2RCKW:inhibitor:E11 DARPin complex were applied to a glow-discharged UltraAuFoil Holey Gold 200 mesh R2/2 grid (Quantifoil, Catalog # Q250AR2A) and incubated in a FEI Vitrobot IV chamber at 4°C and 95% humidity for 20 sec ...
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bioRxiv - Biochemistry 2023Quote: 2.4 µL of sample were applied to a freshly glow discharged 200-mesh Cu + 2 nm C R1.2/1.3 grid (Quantifoil Micro Tools), blotted for 6 s at 100% humidity ...
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bioRxiv - Biochemistry 2022Quote: ... 2–2.5 µl of protein solution was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3 Au 200 mesh). The endogenous samples were applied onto grids containing an additional thin layer of continuous carbon support (Quantifoil R1.2/1.3 Cu 200 mesh + 2 nm C ...
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bioRxiv - Cell Biology 2024Quote: HeLa WT cells or HeLa cells stably expressing Apaf1-GFP were plated on 200 mesh gold EM grids with a holey carbon film R2/2 (Quantifoil). HeLa WT cells were transfected with Apaf1-SNAP-tag ...
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bioRxiv - Microbiology 2024Quote: ... A 4.5 µL droplet of the culture was applied to glow discharged copper grids (Quantifoil R2/2, Quantifoil Micro Tools) and blotted for 2.5 s in a 95% humidity chamber at 20 °C ...
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bioRxiv - Microbiology 2024Quote: ... We initially prepared frozen-hydrated viruses deposited onto Quantifoil R2/2 or R1.2/1.3 grids (Quantifoil Micro tools GmbH, Germany) or glow discharged Lacey grid (Ted Pella Inc. ...
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bioRxiv - Microbiology 2024Quote: ... A 4.5 µL droplet of the culture was applied to glow discharged copper grids (Quantifoil R2/2, Quantifoil Micro Tools) and blotted for 2.5 s in a 95% humidity chamber at 20 °C ...
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bioRxiv - Biochemistry 2024Quote: ... was applied to glow-discharged (GloQube® Plus, Quorum, UK) Quantifoil R1.2/1.3 or R2/2 mesh 200 copper holey carbon grid (Quantifoil, Germany), blotted under 100% humidity at 4°C for 6–8 s ...
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bioRxiv - Microbiology 2024Quote: ... The mixture was added onto glow-discharged copper R2/2 Quantifoil holey carbon grids (Quantifoil Micro Tools GmbH, Jena, Germany). Grids were blotted for 6-8 seconds (under 100% humidity conditions ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µL of 10 µM 11-16Ub[K48]-GFP-35 was pipetted on a holey carbon R2/2 Cu200 grid (Quantifoil) that was plasma cleaned by a SCD005 sputter coater (Bal-Tec ...
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bioRxiv - Cell Biology 2024Quote: ... 3 µL of LRRK2RCKW:RN277:E11 DARPin complex were applied to a glow-discharged UltraAuFoil Holey Gold 200 mesh R2/2 grid (Quantifoil, Q250AR2A) and incubated in a FEI Vitrobot IV chamber at 4 °C and 95 % humidity for 20 sec ...
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bioRxiv - Biochemistry 2024Quote: HeLa cells stably expressing either HRS-mScarlet or HRS-mCherry were grown on 200 mesh gold EM grids with a holey carbon film R2/2 (Quantifoil). Cells were incubated with 50 ng/ml EGF- Alexa 647 ...
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bioRxiv - Biochemistry 2023Quote: ... by applying 3 μL of it to plasma-cleaned 0.6/1 μm holey gold UltrAuFoil grids (Quantifoil). Following a 30 s incubation ...
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bioRxiv - Plant Biology 2021Quote: ... 3 µl of suspended cells in culture medium were applied to the front side of glow-discharged R2/2 carbon-coated copper grids (Quantifoil MicroTools), with additional 1 µL cells added to the back side ...
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bioRxiv - Biochemistry 2021Quote: ... Three microliters of the protein solution were applied on a glow-discharged holey carbon grids (200 mesh Quantifoil R2/2 molybdenum), which had been treated with H2 and O2 mixtures in a Solarus plasma cleaner (Gatan ...
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bioRxiv - Biophysics 2023Quote: ... 3 to 3.5 μl of complex was applied to a glow-discharged UltrAuFoil Holey Gold 200 mesh R2/2 grid (Quantifoil, Catalog # Q250AR2A) and incubated in a FEI Vitrobot IV chamber at 4°C and 95% humidity for 20 sec ...
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bioRxiv - Cell Biology 2022Quote: ... The IL-11Δ10/IL-11RαD1-D3/gp130D1-D3 complex (0.5 mg/mL) was blotted onto UltrAuFoil grids (R2/2, Quantifoil Micro Tools GmbH) that had been subjected to glow discharge (15 mA for 30 s) ...
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bioRxiv - Biochemistry 2024Quote: ... 3 µL of suspended cells in culture medium were applied to the front side of glow-discharged R2/2 carbon-coated copper grids (Quantifoil MicroTools), with additional 1 µL cells added to the back side ...