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Citations for Quantifoil Micro Tools :
101 - 150 of 178 citations for Methylphosphonic Acid 13C 99%; Methyl D3 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: ... bombycis spores (1×108 spores/mL) were placed on grids (Cu R2/1, 200 mesh) (Quantifoil Micro Tools GmbH, Jena, Germany) for 5 min and germinated in 0.1 M KOH for 15 min at 30°C ...
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bioRxiv - Biochemistry 2023Quote: ... at a concentration of around 2.5 mg ml−1 were applied to glow-discharged 300-mesh Au grids (Quantifoil, R1.2/1.3). Excessive sample was removed by blotting with filter paper for 3.5 s before plunge-freezing in liquid ethane using a FEI Vitrobot Mark IV at 100% humidity and 8 °C.
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bioRxiv - Biochemistry 2023Quote: 4 µL of protein solution (total protein concentration = 1 mg/mL) were applied to freshly glow-discharged QUANTIFOIL® R2/1 300 mesh grids (Quantifoil Micro Tools GmbH ...
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bioRxiv - Biochemistry 2024Quote: ... a 3.6 μL aliquot of protein sample at a concentration of 0.12-0.15 mg/mL was pipetted onto a holey carbon film supported by a 200 mesh copper grid (Quantifoil R1.2/1.3). The grid was previously washed and glow discharged (PELCO easiGlow) ...
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bioRxiv - Biochemistry 2024Quote: ... of the freshly eluted complex peak solution (4.0 mg/mL) was loaded onto glow-discharged holey carbon grids (Quantifoil 2/2). The procedures for blotting and plunge freezing the grids were the same as above ...
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bioRxiv - Synthetic Biology 2021Quote: ... 25 mM Tris at pH 8.0 to a concentration of 2 mg/mL to a glow-discharged (1 min at 5 mA) holey carbon grid (Quantifoil Q225-CR1.3). This was followed by plunge-freezing in liquid ethane using an FEI Vitribot Mark IV (100% humidity ...
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bioRxiv - Cell Biology 2019Quote: Freshly purified RS head core complex (0.4 mg/ml) was placed onto glow-discharged grids (Quantifoil R1.2/1.3 200 mesh Cu grids) and plunge-frozen into liquid ethane ...
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bioRxiv - Molecular Biology 2021Quote: ... bremelanotide– or THIQ–bound MC4R–Gs complexes at ∼ 4 mg/mL were applied onto glow-discharged holey carbon grids (Quantifoil R1.2/1.3). Excess samples were blotted away and the grids were vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFischer Scientific).
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bioRxiv - Biophysics 2019Quote: 3.5 μl of microtubule fraction sample (∼4 mg/ml) purified from WT Chlamydomonas was applied to glow-discharged holey carbon grids (Quantifoil R2/2), blotted and vitrified in liquid ethane using the Vitrobot Mark IV (FEI Company) ...
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bioRxiv - Immunology 2019Quote: 4 μL of C5-OmCI-RaCI-CirpT1 in PBS (0.3 mg/ml) was applied to freshly glow-discharged (20 s, 15 mA) carbon-coated 200-mesh Cu grids (Quantifoil, R1.2/1.3). Following incubation for 10 s ...
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bioRxiv - Biochemistry 2020Quote: ... 3 μL of purified AVP-V2R-Gs-Nb35 at 0.75 mg/ml concentration were applied on glow-discharged Quantifoil R 1.2/1.3 300 mesh copper holey-carbon grids (Quantifoil Micro tools GmbH, Germany), blotted for 4.5s and then flash-frozen in liquid ethane using the semi-automated plunge freezing device Vitrobot Mark IV (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: A 2.5-ml suspension of the purified medusavirus particles was applied to a quantifoil grid (R1.2/1.3 Mo) (Quantifoil Micro Tools GmbH, Germany), which was glow-discharged beforehand for 30 s ...
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bioRxiv - Biophysics 2021Quote: Purified HsTMEM120A at 5 mg/ml was applied to a glow-discharged Quantifoil R1.2/1.3 300-mesh gold holey carbon grid (Quantifoil, Micro Tools GmbH, Germany), blotted under 100% humidity at 4°C and plunged into liquid ethane using a Mark IV Vitrobot (FEI).
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bioRxiv - Neuroscience 2020Quote: ... 3.5 uL of sample was applied at a concentration of 3-5 mg/mL to glow-discharged holey carbon grids (Quantifoil R1.2/1.3). The grids were blotted using an FEI Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: 4 µL of 0.06 mg/mL crosslinked complex were applied to glow-discharged holey carbon grids (Quantifoil, QF 1.2/1.3, 300 mesh) covered with a 2 nm carbon layer ...
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bioRxiv - Molecular Biology 2021Quote: The purified tirzepatide–GLP-1R–Gs–Nb35 complex (3 μL at about 20 mg/mL) was applied to a glow-discharged holey carbon grid (Quantifoil R1.2/1.3) and blotted subsequently ...
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bioRxiv - Cell Biology 2020Quote: ... 3.5 µL of gel filtration column eluate (∼0.5 mg/ml) was applied to a holey carbon support (Quantifoil 2/1-4Cu-50) at ∼90% relative humidity ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µL aliquots of 0.1 mg/ml cross-linked ternary complexes were applied to freshly glow-discharged R2/2 Quantifoil 200 mesh copper grids (Quantifoil Micro Tools). Grids were blotted in a climate chamber set to 20 °C and at 95% humidity before plunge frozen in liquid ethane set at −183 °C using a Leica EM GP system (Leica Microsystems) ...
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bioRxiv - Microbiology 2020Quote: ... 3.5 μl of the freshly prepared mixture of the soluble S trimer and monomeric ACE2 (1:3 molar ratio) at ~1 mg/ml was applied to a 1.2/1.3 Quantifoil grid (Quantifoil Micro Tools GmbH, Germany), which had been glow discharged with a PELCO easiGlow™ Glow Discharge Cleaning system (Ted Pella ...
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bioRxiv - Cell Biology 2022Quote: The cryo-EM grid was prepared by applying 3 μl of the protein samples (4 – 6 mg/ml) to glow-discharged Quantifoil R1.2/1.3 300-mesh gold holey carbon grids (Quantifoil, Micro Tools GmbH, Germany). Grids were blotted for 4.0 seconds under 100% humidity at 4°C before being plunged into the liquid ethane using a Mark IV Vitrobot (FEI) ...
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bioRxiv - Biochemistry 2022Quote: ... 20 mg/ml for the D5R-Rotigotine-Gs complex and 15 mg/ml for the D1R-Rotigotine-Gs complex were applied onto a glow-discharged holey carbon grid (Quantifoil R1.2/1.3). Grids were plunge-frozen in liquid ethane using Vitrobot Mark IV (Thermo Fischer Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... An aliquot of 4 μL protein sample of Polθ-HLD-NVB complex at a protein concentration of 0.92 mg/mL was loaded onto a glow-discharged 400 mesh grid (Quantifoil Au R1.2/1.3), blotted with filter paper for 3.0 s and 3 blot force ...
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bioRxiv - Molecular Biology 2024Quote: ... a 2.5 mL suspension of purified medusavirus particles was applied to a glow-discharged Quantifoil grid (R1.2/1.3 Mo; Quantifoil Micro Tools GmbH, Germany). The grid was blotted with filter paper (blotting time ...
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bioRxiv - Biochemistry 2024Quote: ... Peak fractions were concentrated to ∼3.2 mg/mL and 3.5 μL of concentrated sample immediately applied to a Quantifoil R2/1 300 copper mesh grid (Quantifoil Micro Tools GmbH), which had been glow discharged in a GloQube Plus glow discharger for 45 seconds at 25 mA and coated with graphene oxide (Sigma Aldrich ...
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bioRxiv - Biochemistry 2024Quote: 3.5 μL purified mCELSR1 CADH1-GAIN (3.0 mg/mL) was applied on glow-discharged holey carbon grids (Quantifoil R1.2/1.3, 300 mesh), and vitrified using a Vitrobot Mark IV (FEI Company) ...
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bioRxiv - Molecular Biology 2021Quote: The freshly purified complexes (3.0 μL) at a final concentration of 17 mg/mL were applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, 200 mesh), and subsequently vitrified using a Vitrobot Mark IV (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... DRD1-SKF81297-LY3154207-miniGs-Nb35 complex at the concentration of 13 mg mL−1 were applied individually onto glow-discharged holey carbon grids (Quantifoil, Au200 R1.2/1.3) in a Vitrobot chamber (FEI Vitrobot Mark IV) ...
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bioRxiv - Biophysics 2021Quote: ... The SEC-purified complex was concentrated using a 50 kDa cut-off concentrator to 1.5 mg/ml and applied to glow-discharged (0.39 mbar, 15 mA, 30 s) holey carbon grids (QuantiFoil R1.2/1.3 Au 200 mesh). Surplus sample was blotted off for 2-4 seconds with zero blotting force in a controlled environment (4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... aliquots (3 μL) of the purified protein concentrated to ~ 10 mg/mL were placed on glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3). For KCC3-DIOA samples ...
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bioRxiv - Biophysics 2021Quote: The freshly purified AVP–V2R–Gs complex (3.0 μL) at a final concentration of 4.2 mg/mL was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, 300 mesh), and subsequently vitrified using a Vitrobot Mark IV (ThermoFisher Scientific) ...
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bioRxiv - Biophysics 2020Quote: ... 2.5 μl of purified and concentrated sample at a concentration of 1.56 mg/ml was applied to glow-discharged gold grids covered with holey carbon film (Quantifoil, 300 mesh 1.2/1.3) and plunge frozen using a Vitrobot Mark IV ...
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bioRxiv - Biochemistry 2019Quote: ... 2.5 µl of purified proteasome sample (~1.0 mg/ml) was applied to glow-discharged holy carbon grids (Quantifoil 400 mesh Cu R1.2/1.3). For PAN-T20S sample ...
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bioRxiv - Biochemistry 2021Quote: ... 3 μL of protein solution at 5 mg/mL (with 0.025% DDM) was applied onto a glow-discharged holey carbon grid (Quantifoil, 300 mesh, R1.2/1.3). Excess samples were blotted for 5.0 s with a blotting force of 3 ...
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bioRxiv - Biophysics 2021Quote: ... 3 μl of purified ghrelin-bound complex at 13 mg/ml and GHRP-6-bound ghrelin receptor complex at 8 mg/ml were applied individually onto a glow-discharged holey carbon grid (Quantifoil, Au300 R1.2/1.3) in a Vitrobot chamber (FEI Vitrobot Mark IV) ...
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bioRxiv - Microbiology 2022Quote: Three-microliter aliquots of all AdSDV capsid populations (~1 mg/mL) were applied to glow-discharged Quantifoil holey carbon grids with a thin layer of carbon (Quantifoil Micro Tools GmbH) and vitrified using a Vitrobot Mark IV (FEI ...
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bioRxiv - Molecular Biology 2022Quote: The purified complex samples (3 μL at 5-8 mg/mL) were applied to glow-discharged holey grids (Quantifoil R1.2/1.3, 300 mesh) and subsequently vitrified using a Vitrobot Mark IV (ThermoFisher ...
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bioRxiv - Biochemistry 2023Quote: 3.5 µl purified TOL-1–LAT-1 full-ectodomain complex (0.28 mg/ml) was applied on glow-discharged holey carbon grids (Quantifoil R1.2/1.3, 200 mesh), and vitrified using a Vitrobot Mark IV (FEI Company) ...
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bioRxiv - Plant Biology 2023Quote: Three µl of purified PSI at a concentration of 3 mg/ml was applied to the glow-discharged holey carbon grids (Cu QUANTIFOIL® R1.2/1.3), then vitrified using a Leica GP2 plunge freezer (2.5 s blot ...
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bioRxiv - Cell Biology 2023Quote: 4 ul of Nannochloropsis suspensions (4 x 107 cells mL−1) were pipetted onto a 300 mesh gold Quantifoil grid with 2um holes (Quantifoil Micro Tools, GmbH) and manually blotted from the opposite side in a Vitrobot Mark IV (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Sample volumes of 3.5 µl (8 OD260 per ml) were applied to grids (Quantifoil, Cu, 300 mesh, R3/3 with 3 nm carbon) which had been freshly glow-discharged using a GloQube (Quorum Technologies ...
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bioRxiv - Biochemistry 2023Quote: 4 μL of Fcα–J–FcRL4 (0.3 mg/mL) were applied to glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3, 300 mesh) or Ni-Ti grids (CryoMatrix ...
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bioRxiv - Biophysics 2021Quote: 3 μL aliquots of purified tau filaments from PSP human brain tissue (~0.006 mg/mL) were applied to glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3, 300 mesh), blotted with filter paper and plunge-frozen into liquid ethane using an FEI Vitrobot Mark IV ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 μl of protein sample (about 0.73 mg/ml) was applied onto a glow-discharged holey carbon grid (Quantifoil Au, R2/2, 300 mesh). After blotting for 3 s ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 μl of protein sample (about 1.5 mg/ml) was applied onto a glow-discharged holey carbon grid (Quantifoil Au, R1.2/1.3, 300 mesh). At a temperature of 4°C and under a humidity of 100% ...
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bioRxiv - Microbiology 2019Quote: 3 μl of purified complex at 1 to 3.6 mg/ml were applied to glow-discharged holey carbon-coated grids (Quantifoil 300 mesh, Au R1.2/1.3). Grids were blotted for 3 s at 100% humidity at 22 °C and frozen in liquid ethane using a Vitrobot Mark IV (FEI) ...
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bioRxiv - Molecular Biology 2019Quote: ... 2.4 μl of 0.12 mg/ml of the protein preparation were applied to glow-discharged holey carbon grids (Quantifoil, QF 2/1, 300 mesh) covered with a 2 nm carbon layer ...
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bioRxiv - Molecular Biology 2019Quote: ... 2.8 µl of 0.10 mg/ml of the protein preparation were applied to glow-discharged holey carbon grids (Quantifoil, QF 2/1, 300 mesh) covered with a 2 nm carbon layer ...
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bioRxiv - Biochemistry 2020Quote: ... 2.5 μL aliquots of purified Dnf1p–Lem3p or Dnf2p–Lem3p at a concentration of about 1.5 mg/mL were placed on glow-discharged holey carbon grids (Quantifoil Au R2/2, 300 mesh) and were flash-frozen in liquid ethane using a FEI Vitrobot Mark IV ...
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bioRxiv - Biophysics 2021Quote: ... 3 μL of the purified lasmiditan–5-HT1F–Gi complex at concentration ∼15 mg/ml were applied onto a glow-discharged holey carbon grid (Quantifoil R1.2/1.3 Au 300). Grids were plunge-frozen in liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... 3.5 μL of purified CB1-Gi complex at 12 mg/ml was applied on glow- discharged holey carbon gold grids (Quantifoil R1.2/1.3, 200 mesh) at room temperature (for AM11517) ...