Labshake search
Citations for Quantifoil Micro Tools :
51 - 100 of 178 citations for Methylphosphonic Acid 13C 99%; Methyl D3 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... 3 μL of the purified complex (8 mg/ml) was deposited onto a glow-discharged holey grid (Quantifoil Au 300 mesh R1.2/1.3 ...
-
bioRxiv - Biochemistry 2023Quote: ... the protein was diluted from 0.1 mg/ml to 0.05 mg/ml with deionized water and loaded onto a freshly glow-discharged Quantifoil R1.2/1.3 Cu grid (Quantifoil Micro Tools GmbH ...
-
bioRxiv - Biophysics 2021Quote: ... A volume of 4 μL cell suspension at a concentration of 3.07-3.5×107 cells/ml was directly applied to glow-discharged holey carbon R2/1 Cu 200 mesh grids (Quantifoil). After 5 s back-side blotting ...
-
bioRxiv - Biophysics 2020Quote: Three microliters of purified HCoV-NL63 virions (4.5 mg/mL) were first applied to 300-mesh R2/1 + 2 nm C-film grids (Quantifoil) that had been glow-discharged for 15s PELCO easiGlow (TED PELLA ...
-
bioRxiv - Biophysics 2020Quote: ... This sample was blotted once (3.5 mL per blot) onto glow discharged 200 mesh Quantifoil 1.2/1.3 grids (Quantifoil Micro Tools) at 4°C and 100% humidity and plunge frozen in liquid ethane cooled to the temperature of liquid nitrogen (Thermo Fisher Vitrobot Mark IV) ...
-
bioRxiv - Biochemistry 2019Quote: ... 3 μl of ∼0.06 mg/ml TorsinA polymers were applied to glow-discharged Quantifoil R1.2/1.3 400 mesh Cu holey carbon grids (Quantifoil, Germany), blotted (8 seconds ...
-
bioRxiv - Molecular Biology 2020Quote: ... 4 μl of purified PA7LF3 at a concentration of ∼0.06 mg/ml was applied onto freshly glow discharged grids (Quantifoil R 1.2/1.3 holey carbon with a 2 nm additional carbon support ...
-
bioRxiv - Biophysics 2021Quote: The ScBfr sample at concentration of 1.8 mg/ml was blotted (3.5□μl per blot) onto Au 300 mesh Quantifoil 1.2/1.3 grids (Quantifoil Micro Tools), and immediately plunge frozen using a Vitrobot (FEI) ...
-
bioRxiv - Microbiology 2022Quote: ... Purified BfpD was diluted to 4.5 mg/ml and 3 µl was applied to a glow-discharged 300 mesh UltraAufoil -1.2/1.3 holey-gold (Quantifoil, Germany). Grids were blotted for 2 s with ash-free Whatman® Grade 540 filter paper in a Vitrobot Mark IV (ThermoFisher Scientific ...
-
bioRxiv - Biophysics 2022Quote: Eleven cryoEM grids were prepared by two people by adding 3 μL of mouse apoferritin (mApof, from Dr. Kikkawa’s lab) solution (8 mg/mL) to UltrAuFoil 1.2/1.3 grids (Quantifoil, Jena, Germany) immediately after plasma cleaning (Gatan Solarus II plasma cleaner ...
-
bioRxiv - Biophysics 2023Quote: ... 3 μL HA trimer (0.75 mg/ml) was applied to holey carbon grid (Quantifoil 400 mesh Au R1.2/1.3) which was glow discharged 30 s at 15 mA ...
-
bioRxiv - Biophysics 2023Quote: ... 3 μL apoferritin (0.67 mg/ml) was loaded on the holey carbon grids (Quantifoil 400 mesh Cu R1.2/1.3) which was glow discharged for 30s ...
-
bioRxiv - Biophysics 2023Quote: ... 3 μL HA trimer (0.75 mg/ml) was applied to holey carbon grid (Quantifoil 400 mesh Cu R1.2/1.3) which was glow discharged 30 s at 15 mA ...
-
bioRxiv - Biophysics 2023Quote: ... 4 μl of 4.3 mg/ml Slo1-γ1 complex were applied to glow-discharged UltrAuFoil R2/2 200 grid (Quantifoil). Any excess liquid was removed by blotting for 3.5 s at a blot force of −3 ...
-
bioRxiv - Neuroscience 2023Quote: ... Double application of 3 µL protein (∼0.6 mg/mL) SYM2081 complex was carried out on glow-discharged gold grids (R 0.6/1, 300 mesh, Quantifoil). The grids were blotted for 4.5 s and 3.5 s ...
-
bioRxiv - Microbiology 2023Quote: ... 3 μL of 3 mg/mL of protein was added to glow discharged UltrAuFoil holey gold grid (Quantifoil GmbH) using a Vitrobot Mark IV (Thermofisher ...
-
Molecular insights into the atypical activation mechanism of GPR156 in maintaining auditory functionbioRxiv - Molecular Biology 2024Quote: ... Gi3-bound: 4.5 mg/ml) was applied onto the glow-discharged holey carbon grids (Quantifoil, R1.2/1.3, 300 mesh). The grids were blotted for 3.0 s with a blot force of 10 at 4 ℃ ...
-
bioRxiv - Biophysics 2021Quote: 4 µL of purified HsPepT1 at 2 mg/mL was applied to a glow discharged gold holey carbon 2/1 300-mesh grid (Quantifoil). The grid was blotted for 4 s at 0 force before being plunge vitrified in liquid propane using a Mark IV Vitrobot (ThermoFisher) ...
-
bioRxiv - Biophysics 2020Quote: ... 3.5 µl of purified channel proteins at ∼6 mg/ml were pipetted onto glow-discharged copper Quantifoil R2/2 holey carbon grids (Quantifoil). Grids were blotted for 2 s at ∼100% humidity and flash frozen in liquid ethane ...
-
bioRxiv - Molecular Biology 2020Quote: ... or the purified apo complex sample at about 0.75mg/ml (combined with 0.5 µL of 0.025% DDM) was applied onto a glow-discharged holey carbon grid (Quantifoil R1.2/1.3), blotted ...
-
bioRxiv - Biochemistry 2020Quote: ... at ∼0.3 mg/ml was applied to a 1.2/1.3 Quantifoil grid with continuous carbon support (Quantifoil Micro Tools GmbH), which had been glow discharged for 60 s at 15 mA ...
-
bioRxiv - Biochemistry 2020Quote: ... 3.5 μl of the peak fraction from gel filtration chromatography at ~1.0 mg/ml was also applied to the glow discharged 1.2/1.3 Quantifoil grids (Quantifoil Micro Tools Gmb). Grids were immediately plunge-frozen in liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific) ...
-
bioRxiv - Biochemistry 2020Quote: ... 3.5 μl of the freshly purified G614 sample in NP-40 at ~0.3 mg/ml was applied to a 1.2/1.3 Quantifoil grid with continuous carbon support (Quantifoil Micro Tools GmbH), which had been glow discharged with a PELCO easiGlow™ Glow Discharge Cleaning system (Ted Pella ...
-
bioRxiv - Biochemistry 2021Quote: ... 3.5 μl of the freshly purified sample from the peak fraction in DDM at ~2.0 mg/ml for the B.1.1.7 protein or ~1.5 mg/ml for the B.1.351 protein was applied to a 1.2/1.3 Quantifoil grid (Quantifoil Micro Tools GmbH), which had been glow discharged with a PELCO easiGlow™ Glow Discharge Cleaning system (Ted Pella ...
-
bioRxiv - Biophysics 2020Quote: ... 3.5 μL at 2.5 mg/ml sample was directly applied to glow-discharged 200 mesh carbon grids (Quantifoil R1.2/1.3). Grids were immediately plunge-frozen in liquid ethane using a FEI Vitrobot Mark IV (Thermo Fisher Scientific ...
-
bioRxiv - Plant Biology 2022Quote: 3 μL of purified PSI complex at 1 mg Chl mL−1 were applied to glow-discharged (GloQube Quorum, 40 sec, 20mA) holey carbon grids (Quantifoil 300 Au R1.2/R1.3 ...
-
bioRxiv - Biochemistry 2022Quote: 3 µl of prepared complexes at a concentration of approximately 1 mg/ml was applied to a glow-discharged Quantifoil 300 mesh copper R1.2/1.3 grids (Quantifoil Micro Tools). The grids were then flash frozen in liquid ethane using a Thermo Fisher Scientific Vitrobot IV (0-0.5 s waiting time ...
-
bioRxiv - Biochemistry 2019Quote: ... 2.5μl (~0.1 mg/ml) was applied onto amino-functionalized graphene oxide holy carbon grids (Quantifoil 300 mesh Au R1.2/1.3). The grids were blotted by Whatman No ...
-
bioRxiv - Biochemistry 2020Quote: ... at 4.0 mg/ml and INT-777–GPBAR–Gs complex (3.0 μL) at 4.5 mg/ml were applied onto a glow-discharged holey carbon grid (Quantifoil R1.2/1.3), and subsequently vitrified using a Vitrobot Mark IV (Thermo Fischer Scientific) ...
-
bioRxiv - Biophysics 2022Quote: 3.5 μl of protein samples at 4-6 mg/ml were applied to glow-discharged QF R1.2/1.3 300 mesh gold grids (Quantifoil, Großlöbichau, Germany). Grids were blotted for 3 s at 4°C and 100% humidity and plunge-frozen into liquid ethane using FEI Mark IV Vitrobot (FEI ...
-
bioRxiv - Microbiology 2022Quote: ... the purified full-length BA.2 spike protein at 4.6 mg/ml in DDM was applied to a 1.2/1.3 Quantifoil gold grid (Quantifoil Micro Tools GmbH), which had been glow discharged with a PELCO easiGlow™ Glow Discharge Cleaning system (Ted Pella ...
-
bioRxiv - Microbiology 2023Quote: Cryo-EM grids were prepared by applying 3.5 μL of concentrated sample at 1.2 mg/mL to glow discharged 400 mesh R1.2/1.3 UltrAuFoil grids (Quantifoil Micro Tools GmbH). The sample was adsorbed for 30 s on the grids followed by plunge freezing in liquid ethane using a Vitrobot Mark IV plunge freezer (Thermo Fischer Scientific) ...
-
bioRxiv - Biochemistry 2023Quote: ... 3 μl of protein at a concentration of approximately 10 mg ml−1 were loaded onto glow-discharged holey carbon grids (Quantifoil Au 300 mesh R1.2/1.3 or Quantifoil Cu/Rh 300 mesh R1.2/1.3) ...
-
bioRxiv - Biochemistry 2022Quote: ... the postfusion S-nanodisc sample at 1.5 mg/ml was applied to a 1.2/1.3 Quantifoil gold grid (Quantifoil Micro Tools GmbH), which had been glow discharged with a PELCO easiGlow™ Glow Discharge Cleaning system (Ted Pella ...
-
bioRxiv - Biophysics 2023Quote: ... 3.5 μL of labeled GltPh protein (4.5 mg/mL) was applied to a glow-discharged QuantiFoil R1.2/1.3 300-mesh gold grid (Quantifoil Micro Tools GmbH) and incubated for 2 min under 100 % humidity at the desired temperatures ...
-
bioRxiv - Molecular Biology 2023Quote: Four μL of 0.5 mg/ml CRY:TIM solution was applied to glow-discharged grids (300 mesh Quantifoil Au, R1.2/1.3) that were purchased from Electron Microscopy Sciences ...
-
bioRxiv - Biophysics 2023Quote: ... HeLa cells were trypsinized and seeded at a density of 0.5 – 0.7 X 105 cells/mL on carbon-coated gold Quantifoil grid (200 mesh, R 2/1, holey SiO2 film; Quantifoil Micro Tools GmbH ...
-
bioRxiv - Biophysics 2023Quote: ... 4 µL fresh sample (0.48 mg/mL) was applied on glow-discharged Au holey carbon grids (Quantifoil R 0.6/1) and the following operation were the same as describe above for preparing the sample of gMCM8/9 complex.
-
bioRxiv - Cell Biology 2023Quote: 3 µl of prepared complexes at a concentration of approximately 1 mg/mL was applied to UltrAuFoil 300 mesh gold R1.2/1.3 grids (Quantifoil Micro Tools), freshly glow discharged with an Edwards S150B glow discharger for 1 min 15 sec ...
-
bioRxiv - Immunology 2023Quote: ... 3 μL of the purified complex (8 mg/ml) was deposited onto a glow-discharged holey grid (Quantifoil Au 300 mesh R1.2/1.3, Quantifoil, Großlöbichau, Germany). It was then plunge-frozen in liquid ethane using the Vitrobot Mark IV (Thermo Fischer Scientific) ...
-
A distinctive ligand recognition mechanism by the human vasoactive intestinal polypeptide receptor 2bioRxiv - Molecular Biology 2021Quote: The purified PACAP27–VIP2R–Gs complex (3 μL at 3.7 mg per mL) was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3). Vitrification was performed using a Vitrobot Mark IV (ThermoFisher Scientific ...
-
bioRxiv - Biochemistry 2019Quote: ... Samples of the freshly prepared GFLV-Nb23 complex (2.5 µl of a 0.5 mg/mL solution) were applied to 300 mesh holey carbon Quantifoil R1.2/1.3 grids (Quantifoil Micro Tools, Jena, Germany), blotted with filter paper from both sides in the temperature- and humidity-controlled Vitrobot apparatus (FEI ...
-
bioRxiv - Microbiology 2021Quote: ... 3μl of protein solution at a concentration of 0.2 mg/ml were deposited on glow-discharged Quantifoil R 1.2/1.3 holey carbon grids (Quantifoil Micro Tools GmbH, Germany). Samples were manually blotted for four seconds at room temperature and vitrified by rapidly plunging into liquid ethane using a home-built plunging apparatus ...
-
bioRxiv - Biophysics 2019Quote: 3.5 ul of sample of doublets (∼4 mg/ml) was applied to a glow-discharged holey carbon grid (Quantifoil R2/2), blotted and plunged into liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2019Quote: We applied 3.2 μL gp160:PG16 Fab complex (at 0.3 mg/mL) to Quantifoil R 1.2/1.3 holey carbon 200-mesh copper grids (Quantifoil Micro Tools, Großlöbichau, Germany) or (at ∼0.015 mg/mL ...
-
bioRxiv - Biochemistry 2021Quote: The purified TIP39–PTH2R–Gs complex (3 μL at 8.5 mg per mL) was applied on a glow-discharged holey carbon grid (Quantifoil R1.2/1.3). Vitrification was performed using a Vitrobot Mark IV (ThermoFisher Scientific ...
-
bioRxiv - Biochemistry 2021Quote: ... 3.5 µl of the freshly purified sample from the peak fraction in DDM at ∼2.5 mg/ml for the Delta variant or ∼2.0 mg/ml for the Gamma and Kappa variants was applied to a 1.2/1.3 Quantifoil grid (Quantifoil Micro Tools GmbH), which had been glow discharged with a PELCO easiGlowTM Glow Discharge Cleaning system (Ted Pella ...
-
bioRxiv - Microbiology 2020Quote: Three μl of purified HKU1 HE (4 mg/ml) was dispensed on Quantifoil R1.2/1.3 200-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly glow discharged for 30 seconds at 20 mA using GloQube Glow Discharge system (Quorum Technologies) ...
-
bioRxiv - Molecular Biology 2020Quote: ... 3 µl of the purified antagonist bound GABAB heterodimer or baclofen/BHFF bound–GABAB–Gi1 complex at ∼2.0 mg/ml was applied onto a glow-discharged 200 mesh holey carbon grid (Quantifoil R1.2/1.3). The grids were blotted and then plunge-frozen in liquid ethane using Vitrobot Mark IV (Thermo Fischer Scientific) ...
-
bioRxiv - Biophysics 2022Quote: A 3 µl aliquot of freshly concentrated or thawed protein at ∼1 mg/ml was deposited on an UltraAuFoil 300 mesh R 0.6/1 grid (Quantifoil Inc., UK) at 4 °C and 100% humidity in a Vitrobot Mark IV robot (Thermo Fisher) ...