Labshake search
Citations for Quantifoil Micro Tools :
601 - 650 of 658 citations for L β Homo β 2 hydroxyphenyl glycine; R 3 Amino 3 2 hydroxyphenyl propanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: An aliquot (2.5 µL) of the purified tokyovirus particles was placed onto R 1.2/1.3 Quantifoil grids (Quantifoil Micro Tools) that were glow-discharged using a plasma ion bombarder (PIB-10 ...
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bioRxiv - Neuroscience 2024Quote: ... Double application of 3 μL protein (∼0.6 mg/mL) SYM2081 complex was carried out on glow-discharged gold grids (R 0.6/1, 300 mesh, Quantifoil). The grids were blotted for 4.5 s and 3.5 s ...
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bioRxiv - Microbiology 2024Quote: ... after applying 2.5 µl of sample to a cryoEM grid (Quantifoil Cu/Rh R3.5/1, UltrAuFoil R 1.2/1.3) as described previously56.
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bioRxiv - Microbiology 2021Quote: ... Aliquot (2.5 μL) of the sample was placed onto a holey-carbon copper grid (Quantifoil Micro Tools, R 1.2/1.3) previously glow-discharged in a plasma ion bombarder (PIB-10 ...
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High-resolution model of Arabidopsis Photosystem II reveals the consequences of digitonin-extractionbioRxiv - Plant Biology 2021Quote: ... 4 μl concentrated sample was applied to glow-discharged (30 s at 50 mA) Quantifoil R 1.2/1.3 Cu 300 grids (Quantifoil Micro Tools) and grids were plunge frozen in liquid ethane using an FEI Vitrobot MkIV (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Vitrified specimen was prepared by applying 3 μl of protein complexes onto a glow-discharged 300 mesh gold holey carbon Quantifoil R 1.2/1.3 grid (Quantifoil Micro Tools) and frozen in liquid ethane cooled by liquid nitrogen inside a Vitrobot Mark IV (FEI) ...
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bioRxiv - Biophysics 2023Quote: ... 4 µL fresh sample (0.48 mg/mL) was applied on glow-discharged Au holey carbon grids (Quantifoil R 0.6/1) and the following operation were the same as describe above for preparing the sample of gMCM8/9 complex.
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bioRxiv - Microbiology 2023Quote: ... 2 μL of sample was applied on each side of a freshly glow-discharged QUANTIFOIL R 1.2/1.3 Cu 200-mesh grid (Quantifoil Micro Tools). The grid was blotted at once for 15 s from the back side and plunged into liquid ethane using EM GP2 (Leica Microsystems ...
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bioRxiv - Cell Biology 2024Quote: Cells were seeded and grown overnight on Quantifoil carbon film gold grids (Quantifoil R 1/4 on 200 gold mesh) coated with 10 µg/ml FN ...
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bioRxiv - Biophysics 2023Quote: We applied 3 μl of purified MeFDH1 (0.5 mg/ml) to a 1.2/1.3 holey carbon 200 mesh grid (Quantifoil), blotted for 4 s in a 100% humidity environment at 12 °C and then froze the specimens by plunging them into liquid ethane using a Vitrobot Mark IV (FEI).
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bioRxiv - Biochemistry 2023Quote: ... 300-mesh R0.6/1.0 holey carbon copper grid (preprocessed with 0.1% poly L-lysine hydrobromide after glow-discharging) (Quantifoil), or an R1.2/1.3 amorphous nickel titanium alloy (ANTA ...
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bioRxiv - Biochemistry 2022Quote: ... with a surface-active additive (0.01% (w/v) glycyrrhizic acid) was added to a glow discharged (PELCO easiGlow) holey gold grid (Quantifoil UltrAuFoil grids (R0.6/1 ...
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bioRxiv - Plant Biology 2020Quote: ... 4 μL of cell culture was blotted onto R 1/4 SiO2-coated 200-mesh gold EM grids (Quantifoil Micro Tools) and plunge frozen in a liquid ethane/propane mixture ...
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bioRxiv - Microbiology 2021Quote: ... 3μl of protein solution at a concentration of 0.2 mg/ml were deposited on glow-discharged Quantifoil R 1.2/1.3 holey carbon grids (Quantifoil Micro Tools GmbH, Germany). Samples were manually blotted for four seconds at room temperature and vitrified by rapidly plunging into liquid ethane using a home-built plunging apparatus ...
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bioRxiv - Biochemistry 2021Quote: A 3 μL aliquot of mutant mTORC1 A1459P/DEPTOR complex was added onto UltrAuFoil R 1.2/1.3 Au 300 mesh (Quantifoil Micro Tools GmbH) after the grid was glow-discharged using an Edwards Sputter Coater S150B for 60 s at 40 mA ...
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bioRxiv - Biophysics 2022Quote: A 3 µl aliquot of freshly concentrated or thawed protein at ∼1 mg/ml was deposited on an UltraAuFoil 300 mesh R 0.6/1 grid (Quantifoil Inc., UK) at 4 °C and 100% humidity in a Vitrobot Mark IV robot (Thermo Fisher) ...
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bioRxiv - Biophysics 2023Quote: ... 3.5 μl of concentrated purified protein was applied to glow-discharged copper holey carbon grids (Quantifoil R 1.2/1.3, 300 mesh). In all cases ...
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bioRxiv - Biophysics 2020Quote: ... and 3 μl of the diluted sample was applied onto glow-discharged R1.2/1.3 300 mesh UltrAuFoil gold grids (Quantifoil) for 30 s and then blotted for 5 s before plunge-freezing the grids into liquid ethane cooled by liquid nitrogen ...
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bioRxiv - Biophysics 2021Quote: ... 3.5 μL of the mixture was applied onto a glow-discharged Quantifoil R1.2/1.3 400 mesh Au grid (Quantifoil), blotted for 4 s at room temperature (RT ...
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bioRxiv - Biophysics 2020Quote: ... 3.5 µL of the assembled filament were applied to glow-discharged R1.2/1.3 Quantifoil 200 Au mesh grids (Quantifoil) in a Mark IV Vitrobot (FEI) ...
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bioRxiv - Biophysics 2022Quote: ... 4 µL of the freshly prepared mixture were applied to glow-discharged (Quorum GloQube) R1.2/1.4 Cu 200 (Quantifoil) grids ...
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bioRxiv - Microbiology 2022Quote: ... A 4 μL droplet of the sample was applied onto a R1.2/1.3 300-mesh copper holey carbon grid (Quantifoil), previously glow discharged for 30 s at 30 mA using a GloQube system (Quorum Technologies) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 3 μl of the protein complex was applied onto 300 mesh R1.2/1.3 UltrAuFoil Holey gold support films (Quantifoil). For cryo-EM grids preparation of DHA-FFA4-miniGq complex ...
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bioRxiv - Microbiology 2022Quote: ... 3.5 μl sample were adsorbed for 45 s on glow-discharged 400 mesh R1.2/R1.3 holey carbon grids (Quantifoil) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... 3 μl of parasites were applied onto a freshly plasma-cleaned UltrAufoil R1.2/1.3 300 mesh EM grid (Quantifoil) in a humidity controlled facility ...
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bioRxiv - Biophysics 2024Quote: ... and 3 µl of the diluted sample was applied onto glow-discharged R1.2/1.3 300 mesh UltrAuFoil gold grids (Quantifoil) (Russo and Passmore ...
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bioRxiv - Biophysics 2024Quote: ... 3 μL of the mixture was applied to a glow-discharged grid (R1.2/1.3 300 mesh Au grid, Quantifoil), blotted for 2 s with force 0 and plunged into liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2024Quote: ... 3 µL of sample was rapidly applied to a freshly glow-discharged UltrAuFoil R1.2/1.3 300 mesh grids (Quantifoil), blotted with Whatman No ...
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bioRxiv - Biochemistry 2020Quote: ... 3 μL of purified AVP-V2R-Gs-Nb35 at 0.75 mg/ml concentration were applied on glow-discharged Quantifoil R 1.2/1.3 300 mesh copper holey-carbon grids (Quantifoil Micro tools GmbH, Germany), blotted for 4.5s and then flash-frozen in liquid ethane using the semi-automated plunge freezing device Vitrobot Mark IV (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... 4 µL of cell suspension was applied on 200-mesh R ¼ SiO2-film covered gold grids or 200-mesh R2/1 carbon-film covered copper grids (Quantifoil Micro Tools) (for P ...
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bioRxiv - Cell Biology 2022Quote: Tfam+/− MEFs (TFAM Het MEF) and SV40 mouse embryonic fibroblasts (SV40 MEF) were cultured on R ¼ Carbon 200-mesh gold electron microscopy grids (Quantifoil Micro Tools) and plunge frozen in a liquid ethane/propane mixture using a Vitrobot Mark 4 (Thermo Fischer Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... Protein-DNA complexes were applied to ultra-gold foil grids with holey gold support and 300 mesh size (R 0.6/1,Quantifoil Micro Tools GmbH); grids were flash-frozen as described [21] and were stored in liquid nitrogen until use.
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bioRxiv - Biochemistry 2024Quote: ... 3 µL of proteins / proteins-phospholipids mix samples were gently deposited on glow discharged Quantifoil R 1.2/1.3 holey carbon grids (Quantifoil Micro Tools GmbH, Germany). Samples were manually blotted for four seconds at room temperature and vitrified by rapidly plunging into liquid ethane using a home-built plunging apparatus ...
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bioRxiv - Plant Biology 2021Quote: 4 μl of GmQUAC1malate proteins (purified in 75 mM L-malate) were applied to newly glow-discharged holy carbon film grids (Au R1.2/1.3, 300 meshes, Quantifoil, Germany). The grids were blotted with force 3 and blotting time of 9.0 s at 100% humidity and 4°C and were vitrified by plunge freezing into liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 μl of purified PA7LF3 at a concentration of ∼0.06 mg/ml was applied onto freshly glow discharged grids (Quantifoil R 1.2/1.3 holey carbon with a 2 nm additional carbon support ...
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bioRxiv - Cell Biology 2024Quote: ... 3.5 µl of 4 mg/mL proteasomal complexes was applied to glow-discharged R0.6/1 UltrAuFoil 300 mesh grids (Quantifoil) and frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... were applied to glow-discharged (25 mA, 10 s) QuantiFoil Gold R 0.6/1 300-mesh holey carbon grids (QuantiFoil, Micro Tools GmbH, Germany), blotted for 4.5 s ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were diluted twofold with Buffer F and 3.5 µl of sample was immediately applied onto a glow-discharged 300-mesh gold Quantifoil R1.2/1.3 grid (Quantifoil Micro Tools). Grids were blotted for 2-4 s and plunge-frozen in liquid ethane cooled by liquid nitrogen with a Vitrobot MarkIV (ThermoFisher ...
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bioRxiv - Biophysics 2022Quote: ... 3 µl of DeCLIC sample was applied to a glow-discharged quantifoil 1.2/1.3 Cu 300 mesh grid (Quantifoil Micro Tools), which was then blotted for 1.5 s and plunge-frozen into liquid ethane using a FEI Vitrobot Mark IV ...
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bioRxiv - Microbiology 2022Quote: ... 3.0 μL of above-described samples or non-treated CVA9 virions as a control were applied to glow-discharged grids (Quantifoil R1.2/1.3 holey carbon on 300 copper mesh ...
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bioRxiv - Biochemistry 2021Quote: ... 2.5 μL of selected elution fractions were applied to a 300-mesh holey carbon grid (Au 1.2/1.3, Quantifoil Micro Tools), blotted 2 s at 75 % humidity ...
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bioRxiv - Cell Biology 2024Quote: ... 4 μl of the cell suspension was applied onto a glow-discharged holey SiO2 grid (R1/4, Au, 200 mesh, Quantifoil) mounted on in the plunger chamber ...
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bioRxiv - Biochemistry 2023Quote: ... 2.8 µl of F-actin sample was applied to a glow-discharged R2/1 Cu 300 mesh holey-carbon grid (Quantifoil). Excess solution was blotted away and the grids were plunge frozen in liquid ethane or a liquid ethane/propane mixture using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were diluted twofold with the same buffer and 3.5 µl of sample was immediately applied onto a glow-discharged 300-mesh gold Quantifoil R1.2/1.3 grid (Quantifoil Micro Tools). Grids were glow discharged with an Edwards 12E6/531 glow discharger at 30 mA for 60 s ...
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bioRxiv - Biochemistry 2021Quote: ... 3 μL of the ATP13A2 sample were applied to a glow-discharged (PELCO easiGlow; 0.39 mBar, 25–30 mA, 40–45 s) gold holey carbon grid (Quantifoil R 1.2/1.3, 400 mesh). The grid was blotted for 3–4 s and plunge-frozen in liquid-nitrogen-cooled liquid ethane using Vitrobot Mark IV (FEI ...
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bioRxiv - Biophysics 2020Quote: Samples were prepared for CryoEM by applying 5 μl freshly purified Cx46/50-lipid nanodisc complex (∼2.5 mg mL-1) to a glow-discharged holey carbon grid (Quantifoil R 1.2/1.3, 400 mesh) for 10 seconds ...
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bioRxiv - Biochemistry 2022Quote: ... or AE1-Niflumic acid at 5 mg/ml were applied to glow-discharged holey carbon EM grids (Quantifoil 300 copper mesh, R1.2/1.3) in an EM-GP2 plunge freezer (Leica) ...
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bioRxiv - Microbiology 2021Quote: ... 3 μL of sample solution was applied to a Quantifoil holey carbon grid (R1.2/1.3, Mo 200 mesh, Quantifoil Micro Tools GmbH) at 4 °C with 100% humidity ...
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bioRxiv - Microbiology 2020Quote: Three μl of purified HKU1 HE (4 mg/ml) was dispensed on Quantifoil R1.2/1.3 200-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly glow discharged for 30 seconds at 20 mA using GloQube Glow Discharge system (Quorum Technologies) ...
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bioRxiv - Microbiology 2023Quote: A 1.4 μl sample solution was applied onto a glow-discharged holey carbon grid (Quantifoil R1.2/1.3 Mo grid, Quantifoil Micro Tools, Germany), and the grid was plunge-frozen into liquid ethane by Vitrobot mark IV (Thermo Fisher Science ...