Labshake search
Citations for Quantifoil Micro Tools :
551 - 600 of 658 citations for L β Homo β 2 hydroxyphenyl glycine; R 3 Amino 3 2 hydroxyphenyl propanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The endogenous samples were applied onto grids containing an additional thin layer of continuous carbon support (Quantifoil R1.2/1.3 Cu 200 mesh + 2 nm C) and incubated for 30 sec before blotting and freezing ...
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bioRxiv - Neuroscience 2024Quote: ... Supernatant after centrifugation mixed with 10-nm gold beads solution (Aurion, cat # 25486) at 1:1 ratio was added to the mounted grid (Quantifoil R2/2 Cu 200 EM). No other chemicals or stimulation were applied prior to plunge freezing ...
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bioRxiv - Biochemistry 2024Quote: ... or calpain-3-titin/I81-I83 (2mg/mL) from Superose 6 increase 10/300GL column chromatography was loaded onto glow-discharged holey carbon grids (Quantifoil 1.2/1.3 + 2 nm Carbon). The grids were blotted with filter paper for 2.5 secs at 22 °C and then immediately plunged into liquid ethane cooled by liquid nitrogen using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... 4 μl of the extracellular parasites were pipetted onto a glow-discharged (30 seconds at −30 mA) copper R2/2 holey carbon grid (Quantifoil Micro Tools GmbH, Jena, Germany). Samples were back-blotted with a Whatman filter paper (grade 1 ...
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bioRxiv - Microbiology 2024Quote: ... The co-culture-colloidal gold bead mixture was added to glow-discharged copper R2/2 Quantifoil holey carbon grids (Quantifoil Micro Tools GmbH, Jena, Germany). Grids were blotted (under 100% humidity conditions ...
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bioRxiv - Biophysics 2020Quote: ... Protein was frozen on R 1.2/1.3 grids with 300 Au mesh (Quantifoil, Germany). This dataset was collected at UCSF on the Titan Krios 2 ...
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bioRxiv - Biochemistry 2024Quote: ... The 300-mesh Quanti-foil R 1.2/1.3 copper EM grids (Quantifoil Micro Tools) were discharged at 15 mA for 60 seconds using a glow-discharger (PELCO) ...
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bioRxiv - Biochemistry 2022Quote: The samples were frozen on copper or gold grids with holey carbon film coated with a 2 nm continuous carbon film (Quantifoil R1.2/1.3 ultrathin carbon, 300 mesh) freshly glow discharged for 20 s at 15 mA (PELCO easiGlow) ...
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bioRxiv - Biochemistry 2023Quote: ... A sample of 3.0 μM of FH2 and 10.6 μM of actin monomers was applied to holey grids without a carbon film (Quantifoil Au 2/1 300 mesh from EMS). After diluting 4-fold ...
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bioRxiv - Neuroscience 2023Quote: ... 3.5 μL of CopiaGag capsid protein sample was applied to a 300-mesh copper grid with continuous 2 nm carbon film (Electron Microscopy Sciences, QUANTIFOIL® C2-C15nCu30-50) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
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bioRxiv - Biochemistry 2022Quote: ... a 3.0 µL drop was applied to glow discharged holey carbon (R 1.2/1.3; Quantifoil), then blotted for 3 s ...
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bioRxiv - Cell Biology 2022Quote: ... and UV sterilized R ¼ Carbon 200-mesh gold electron microscopy (EM) grids (Quantifoil Micro Tools). After 15-18 hours of culture ...
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bioRxiv - Biophysics 2022Quote: ... except for using copper grid coated with holey carbon (R 1.2/1.3; Quantifoil, Jena, Germany), applying 4 µl virus sample and not adding fiducial beads ...
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bioRxiv - Molecular Biology 2023Quote: ... and vitrification of samples on holey carbon R 1.2/1.3 copper 300 mesh grids (Quantifoil) using a Vitrobot MarkIV device (Thermofisher ...
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bioRxiv - Biophysics 2024Quote: ... 10 second hold) Holey Carbon 300 mesh R 1.2/1.3 gold grid (Quantifoil, Großlöbichau, Germany). After a ∼5 second manual wait time ...
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bioRxiv - Molecular Biology 2024Quote: ... about 0.9 mg/ml for DSS1–INTAC–PEC complex) was applied onto a glow-discharged holey carbon grid (Quantifoil Au, R1.2/1.3 or R2/2, 300 mesh). After blotting for 3 to 6 s ...
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bioRxiv - Biochemistry 2022Quote: Freshly prepared ribosome–Sec61–KZR-8445 complex at 300–500 nM concentration was applied on glow discharged holey-carbon grids (Quantifoil, R1.2/1.3 with a 2-nm carbon film) and flash frozen with an automated plunge freezer (Leica microsystems) ...
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bioRxiv - Plant Biology 2024Quote: 4 μL AtEDS1-AtPAD4-AtADR1 complex purified from Nb leaves was applied to glow-discharged Quantifoil holey carbon grids (Quantifoil Cu R1.2/1.3+2 nm C, 300 mesh). The protein was concentrated to 0.9 mg/mL prior to freezing ...
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bioRxiv - Biophysics 2022Quote: ... the extra carbon coated gold Quantifoil grids (200 mesh, R 1.2/20, holey carbon film; Quantifoil Micro Tools GmbH ...
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bioRxiv - Biochemistry 2020Quote: ... 4 µL of sample was applied to Quantifoil Cu R 1.2/1.3 grids (Quantifoil Microtools GmbH) which has undergone a plasma discharge and were vitrified in liquid ethane using a Vitrobot MkIV plunge freezer (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2020Quote: ... A 2.5-μL aliquot was placed on an R 1.2/1.3 Quantifoil grid (Quantifoil Micro Tools) pre-treated by a glow discharge ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was then applied to a gold Quantifoil R 1.2/1.3 holey carbon grid (Quantifoil) that was glow discharged for 35 sec using PELCO easiGlow glow discharge cleaner ...
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bioRxiv - Bioengineering 2022Quote: ... applied to a holey carbon grid (Ultrafoil 25 nM Au R 1.2/1,3 300 mesh; Quantifoil) pretreated with SAM solution ...
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bioRxiv - Molecular Biology 2023Quote: ... to prepare thin vitreous samples on Quantifoil type R 1.2/1.3 holey-carbon grids (Quantifoil Microtools). The grids were made hydrophilic ...
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bioRxiv - Biochemistry 2021Quote: 2.5 μl of ACE2-RBD-62 complex at 3.5 mg/ml concentration was transferred to glow discharged UltrAuFoil R 1.2/1.3 300 mesh grids (Quantifoil), blotted for 2.5 seconds at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... 1.5 μl of THO*•Sub2 was applied to a glow-discharged UltrAuFoil R 1.2/1.3 grids (Quantifoil). Grids were blotted for 3 s with a blotting force of 3 and 100% humidity at 22 °C and plunged into liquid ethane using an FEI Vitrobot Mark IV (Thermo Fisher).
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bioRxiv - Microbiology 2021Quote: ... 3μL of protein was deposited on a holey copper grids (QUANTIFOIL R 1.2/1.3, 200 mesh, EMS) which had been glow-discharged for 30s at 15 mA (Tedpella Inc) ...
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bioRxiv - Immunology 2021Quote: ... Two types of grids were used: UltrAuFoil R 1.2/1.3 (Au, 300-mesh; Quantifoil Micro Tools GmbH) and Quantifoil R 2/1 (Cu ...
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bioRxiv - Biophysics 2020Quote: 3 ml of apoferritin at 3.8 mg/ml were applied to freshly glow discharged R 1.2/1.3 holey carbon grids (Quantifoil) at 4°C and 100% relative humidity followed by plunge-freezing in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... proper dilutions were made to a final concentration of ∼.7 mg/mL and a 3.0 µL drop was applied to glow discharged holey carbon (R 1.2/1.3; Quantifoil), then blotted for 3 s ...
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bioRxiv - Biochemistry 2021Quote: ... for the BsClpP pH 4.2 sample and negatively glow-discharged holey carbon grid (R 1.2/1.3 Quantifoil) for the BsClpP pH 6.5 sample ...
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bioRxiv - Cell Biology 2022Quote: Vero-E6 were seeded on holey carbon film R 5/20 gold 200 mesh EM grids (Quantifoil Micro Tools GmbH ...
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bioRxiv - Neuroscience 2022Quote: Primary cultured neurons were seeded onto holey gold EM grids (Quantifoil R 1/4 gold 200 mesh). The fiducial marker solution was prepared as for neocortical synaptosomes ...
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bioRxiv - Cell Biology 2024Quote: ... Quantifoil Holey SiO2 R 1/4 Au 200 mesh EM grids (Quantifoil Micro Tools #N1-s13nAi20-01) were glow discharged on both sides in a Pelco easyglow for 45 s at 0.37 mBar and 15 mA ...
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bioRxiv - Molecular Biology 2023Quote: ... applied to a glow-discharged R 1.2/1.3 300 mesh UltrAuFoil gold grid (Quantifoil Micro Tools GmbH) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Scientific ...
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bioRxiv - Biophysics 2023Quote: 3 μl purified MRS2 at approximately 0.5 mg/ml was applied to a glow-discharged 400-mesh R 1.2/1.3 Cu grid (Quantifoil). The cryo grids were blotted for 6 s at 4°C and 95% ...
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bioRxiv - Biophysics 2023Quote: ... 4 µL fresh sample was applied on glow-discharged Cu holey carbon grids (Quantifoil R 1.2/1.3) and incubated for 1 min at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... An aliquot of the incubated mixture (3.5 μl) was applied onto glow-discharged UltrAuFoil 300 mesh R 1.2/1.3 grids (Quantifoil) at ∼4 °C ...
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bioRxiv - Bioengineering 2023Quote: ... These samples were applied onto UltrAuFoil R 1.2/1.3 grids (Au, 300-mesh; Quantifoil Micro Tools GmbH). Prior to sample application the grids were glow-discharged using Ar/O2 plasma for 10 s on a Solarus 950 plasma cleaner (Gatan) ...
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bioRxiv - Biochemistry 2021Quote: ... 3 microlitres of undiluted eluate and of eluate which was diluted 1:2 or 1:4 in buffer A200 were blotted onto holey carbon R 1.2/1.3 copper 300 mesh grids (Quantifoil) and vitrified in liquid ethane using a Vitrobot MarkIV device (Thermofisher) ...
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bioRxiv - Molecular Biology 2022Quote: Purified SARS-CoV-2 spike samples in 10mM Hepes pH7.2 and 150mM NaCl (3 μL at 0.62-0.75mg/ml) were kept at 4C until their application onto QUANTIFOIL R 1.2/1.3 Cu:300-mesh grids (QUANTIFOIL). The grids were vitrified using a Leica GP automatic vitrification robot (Leica) ...
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bioRxiv - Biochemistry 2022Quote: ... 3 μl of sample was applied to a plasma treated gold coated R 1.2/1.3 300 mesh holey carbon grid (Quantifoil), with a blot force of 0 ...
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bioRxiv - Immunology 2022Quote: ... 3 μL of the DCFHP proteins were applied onto a glow-discharged Quantifoil R 1.2/1.3 Cu 300-mesh grid (Quantifoil). The grids were blotted for 2 sec at 20°C and 100% humidity and rapidly cryocooled in liquid ethane using a Vitrobot Mark IV instrument (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... 3μL of protein was deposited on holey carbon copper grids (QUANTIFOIL R 1.2/1.3-2nm, 300 mesh, EMS) which had been glow-discharged for 20s at 15 mA using PELCO easiGlow (TedPella Inc) ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μl of sample was applied to a plasma treated gold coated R 1.2/1.3 300 mesh holey carbon grid (Quantifoil), with a blot force of 0 ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 ul aliquot of the mixture was adsorbed onto a glow-discharged Quantifoil R 1.2/1.3 300 mesh grid (Quantifoil) and vitrified in liquid ethane with a Leica EM GP2 cryoplunger (Leica ...
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bioRxiv - Biochemistry 2024Quote: ... 3.2 μl samples of the solution were applied onto EM grids (Quantifoil, R 1.2/1.3 Cu 300 mesh) that had been glow discharged with PELCO easiGlow for 60 seconds in Vitrobot Mark IV chamber at 4°C and 90% humidity ...
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bioRxiv - Microbiology 2024Quote: ... was applied to previously glow discharged R 1.2/1.3 Molybdenum 200 grids with a holey carbon support film (Quantifoil). Grids were quickly transferred to an EM GP Leica for blotting for 1.5-2.5 seconds at 90% humidity and flash frozen in liquid ethane cooled by liquid nitrogen ...
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bioRxiv - Biophysics 2024Quote: ... 10 mM DTT was incubated for 3 days at 30 °C and 2.5 μL of the solution was applied onto a Quantifoil R 1.2/1.3 200 mesh Cu grid (Quantifoil) and plunge-frozen in liquid ethane by a Vitrobot Mark IV (FEI) ...
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bioRxiv - Molecular Biology 2021Quote: Purified mtLSU or 55S mitoribosome samples (4 μL) were applied to freshly glow discharged R 3.5/1 holey carbon grids (Quantifoil) that were precoated with a 2-3 nm carbon layer using a Leica EM ACE600 coater ...