Labshake search
Citations for Quantifoil Micro Tools :
601 - 650 of 677 citations for 4 2 Hydroxyimino 2 4 methoxy phenyl ethyl piperazine 1 carboxylic acid tert butyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... 200 mesh gold Quantifoil R2/1 grids with a SiO2 support layer (Quantifoil, Cat No N1-S15nAu20-01) were glow discharged and treated with PLL-g-PEG (SuSoS ...
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bioRxiv - Microbiology 2020Quote: ... mobilis Zm4 or Zm6 cells were applied to glow-discharged holey carbon grids (Quantifoil R3.5/1 Cu 200-mesh) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... A volume of 4 μL cell suspension at a concentration of 3.07-3.5×107 cells/ml was directly applied to glow-discharged holey carbon R2/1 Cu 200 mesh grids (Quantifoil). After 5 s back-side blotting ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μL of sample were pipetted onto a freshly glow discharged (PELCO easiGlow, 25 mA, 45 sec) 300 mesh Cu 0.6/1 grid (Quantifoil) and blotted for 4 s at strength 4 ...
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bioRxiv - Microbiology 2024Quote: ... the sample was applied to a holey carbon-coated copper grid for electron microscopy (R2/1, mesh 200; Quantifoil), blotted and vitrified by plunging into liquid ethane using a Vitrobot Mark IV (FEI) ...
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bioRxiv - Immunology 2024Quote: ... The complex (1 mg/mL) was then applied onto the glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3) using an FEI Vitrobot IV ...
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bioRxiv - Neuroscience 2024Quote: ... Double application of 3 μL protein (∼0.6 mg/mL) SYM2081 complex was carried out on glow-discharged gold grids (R 0.6/1, 300 mesh, Quantifoil). The grids were blotted for 4.5 s and 3.5 s ...
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bioRxiv - Microbiology 2024Quote: ... after applying 2.5 µl of sample to a cryoEM grid (Quantifoil Cu/Rh R3.5/1, UltrAuFoil R 1.2/1.3) as described previously56.
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bioRxiv - Biophysics 2024Quote: ... 3.5 µL sample was applied to a freshly glow-discharged (PELCO easiGlow, 30 mA for 120s) 0.6/1 UltrAuFoil grid (Quantifoil) (Russo and Passmore ...
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bioRxiv - Biochemistry 2022Quote: ... An aliquot of 3.5 μl of sample was placed on the glow-discharged cryo-EM grid (Quantifoil R1.2/1.3 or Quantifoil R2/1), blotted and plunge-frozen in liquid ethane using a Mark VI Vitrobot instrument maintained at 100% humidity with blot force 20 and blot time of 3 seconds ...
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bioRxiv - Molecular Biology 2020Quote: ... at 13 °C and 100% humidity: 3 μl of sample were applied onto a freshly glow-discharged copper R2/1 300 mesh grid (Quantifoil), blotted for 8 s on both sides with blotting force −20 and plunge-frozen in liquid ethane.
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bioRxiv - Cell Biology 2021Quote: MEFs expressing emerald-Vimentin are cultured on glow-discharged holey carbon coated EM grids (Au R2/1, 200 mesh, Quantifoil) for 16 h at 37°C in a humidified CO2 incubator ...
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bioRxiv - Biochemistry 2020Quote: ... A volume of 3 μL of the sample was applied to a glow-discharged grid (Quantifoil R2/1 300 mesh) at 25 °C and 90 % humidity ...
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bioRxiv - Cell Biology 2021Quote: ... 3 µl of the samples at 1 µg/µl was applied to UltrAUfoil R1.2/1.3 grids (Quantifoil Micro Tools GmbH). Grids were glow-discharged for 30 sec at 15 mA prior to sample application (PELCO easiGlow™ ...
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bioRxiv - Biochemistry 2022Quote: 3 µl of prepared complexes at a concentration of approximately 1 mg/ml was applied to a glow-discharged Quantifoil 300 mesh copper R1.2/1.3 grids (Quantifoil Micro Tools). The grids were then flash frozen in liquid ethane using a Thermo Fisher Scientific Vitrobot IV (0-0.5 s waiting time ...
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bioRxiv - Biochemistry 2022Quote: 2.8 µl of F-actin sample (3 – 16 µM) was pipetted onto a glow-discharged R2/1 Cu 300 mesh holey-carbon grid (Quantifoil). After incubating for 1 – 2 seconds ...
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bioRxiv - Biochemistry 2021Quote: ... Samples of ΦKZ nvRNAP were adsorbed to a thin film of graphene oxide deposited upon the surface of holey carbon copper grids (R2/1, 300 mesh, Quantifoil). Grids were blotted for 1-2 seconds before plunge freezing in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: An aliquot of 2.5 microliters of purified drCALHM1 sample was applied onto a glow discharged holey carbon film grid (200 mesh, R2/1, Quantifoil). The grid was blotted and flash-frozen in liquid ethane with FEI Vitrobot Mark IV ...
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bioRxiv - Cell Biology 2020Quote: ... Isolated centrioles were then deposited onto 200-mesh copper EM grids coated with holey carbon (R3.5/1, Quantifoil Micro Tools) and plunge-frozen in a liquid ethane/propane mixture ...
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bioRxiv - Cell Biology 2021Quote: K5/K14_1 cells were seeded on glow-discharged Collagen I coated holey carbon gold EM grids (Au R2/1, 200 mesh, Quantifoil) and incubated overnight at 32 °C and in 5% CO2 ...
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bioRxiv - Cell Biology 2020Quote: Samples of concentrated TSCCFL were adsorbed to a thin film of graphene oxide deposited upon the surface of holey carbon copper grids (R2/1, 300 mesh, Quantifoil). Grids were blotted for 2-3 seconds before plunge freezing in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: 3 µl of prepared complexes at a concentration of approximately 1 mg/mL was applied to UltrAuFoil 300 mesh gold R1.2/1.3 grids (Quantifoil Micro Tools), freshly glow discharged with an Edwards S150B glow discharger for 1 min 15 sec ...
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bioRxiv - Biochemistry 2022Quote: ... Three μL of sample at 5 μM was adsorbed onto glow-discharged copper Quantifoil R2/1 holey carbon grids (Quantifoil). Grids were glow discharged 45 seconds at 25 mA using an EMITECH K950 apparatus ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μl of protein at a concentration of approximately 10 mg ml−1 were loaded onto glow-discharged holey carbon grids (Quantifoil Au 300 mesh R1.2/1.3 or Quantifoil Cu/Rh 300 mesh R1.2/1.3) ...
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bioRxiv - Biochemistry 2023Quote: ... 3 µl of the VIF-ΔT solution was applied onto glow-discharged holey carbon EM grids (Cu R2/1, 200 mesh, Quantifoil), which were subsequently blotted manually and plunge frozen in liquid ethane.
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bioRxiv - Biochemistry 2023Quote: ... 2.8 µl of F-actin sample was applied to a glow-discharged R2/1 Cu 300 mesh holey-carbon grid (Quantifoil). Excess solution was blotted away and the grids were plunge frozen in liquid ethane or a liquid ethane/propane mixture using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... 4 µL of chloroplasts solution was placed onto R2/1 or R1.2/1.3 carbon-coated 200-mesh copper EM grids (Quantifoil Micro Tools), blotted for 7-8 seconds from the back side (front side pad covered with a Teflon sheet ...
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bioRxiv - Biophysics 2024Quote: ... 3 µL of purified AE1 at 5 mg/mL was added to a glow discharged (PELCO easiGlow) 0.6/1 µm holey gold grid (Quantifoil UltrAuFoil) and blotted for 4-6 s at 4°C and 100% humidity using the Vitrobot Mark IV system (Thermofisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... gold EM grids with Quantifoil holey carbon film (Quantifoil, R2/1, 300 mesh, gold; Electron Microscopy Sciences, Q3100AR1; or Quantifoil, R3.5/1 ...
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bioRxiv - Bioengineering 2022Quote: ... A total of 3.5 μL of TP–AG treated MT samples was applied to a glow discharged holey carbon grid (Quantifoil R2/1). The grids were blotted ...
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bioRxiv - Cell Biology 2021Quote: ... a thin layer of homemade continuous carbon was floated on top of individual Quantifoil R3.5/1 200 mesh gold grids (Quantifoil Micro Tools). After drying ...
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bioRxiv - Cell Biology 2020Quote: ... 3 μl of samples were applied to glow-discharged Quantifoil R 0.6/1 and R 1.2/1.3 grids (Quantifoil Micro tools GmbH, Germany), blotted for 3 s and then flash frozen in liquid ethane using the automatic plunge freezer EM GP2 at 100% relative humidity ...
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bioRxiv - Cell Biology 2021Quote: MEFs were grown to ~80% confluency on glow-discharged holey carbon EM grids (R2/1, Au 200 mesh; Quantifoil, Jena, Germany) prior to preparation for cryo-ET analysis ...
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bioRxiv - Biochemistry 2023Quote: ... at a concentration of around 2.5 mg ml−1 were applied to glow-discharged 300-mesh Au grids (Quantifoil, R1.2/1.3). Excessive sample was removed by blotting with filter paper for 3.5 s before plunge-freezing in liquid ethane using a FEI Vitrobot Mark IV at 100% humidity and 8 °C.
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bioRxiv - Biophysics 2024Quote: Approximately 3□μL of the sample solution were put on newly glow-discharged TEM grids (Quantifoil R2/1, Cu, mesh 200) and plunge-frozen in liquid ethane using the Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: A 3.5 μL sample of concentrated pNO-decorated CLPs was applied to negatively glow discharged (25 mA, 40 s) R3.5/1 quantifoil copper grids (Quantifoil Micro Tools). The excess of solution was blotted using a Vitrobot Mark IV (FEI ...
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bioRxiv - Biochemistry 2023Quote: MEFs were grown to ∼80% confluency on glow-discharged holey carbon EM grids (R2/1, Au 200 mesh; Quantifoil, Jena, Germany) prior to preparation for cryo-ET analysis ...
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bioRxiv - Neuroscience 2024Quote: Extracted DS13 tau filaments were applied to 300-mesh copper R2/1 holey carbon grids (Quantifoil Micro Tools GmbH, Jena, Germany) that were glow-discharged for 30 s at a current of −30 mA on a PELCO easiGlowTM Glow Discharge Cleaning System ...
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bioRxiv - Microbiology 2024Quote: ... 3.5 uL of the resulting sample were added to glow discharged Quantifoil Ultrathin carbon R2/1 200 mesh EM Grids (Ted Pella, Redding, CA, USA; Quantifoil Micro Tools GmbH ...
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bioRxiv - Microbiology 2020Quote: ... of purified proteins of SARS-CoV2-ST or SARS-CoV2-STN were loaded onto glow-discharged (60 s at 20 mA) holey carbon Quantifoil grids (R2/1, 200 mesh, Quantifoil Micro Tools) using a Vitrobot Mark IV (ThermoFisher Scientific ...
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... A 3.5 µL virus sample solution was applied onto a glow discharged copper grid coated with holey carbon (R2/1; Quantifoil, Jena, Germany) prior to plunge-freezing ...
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bioRxiv - Biophysics 2023Quote: ... 3 μl of medium containing one isolated muscle fibre was placed on the glow-discharged grid (Quantifoil R2/1 Au 200 mesh). After incubation for 60 seconds at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... Peak fractions were concentrated to ∼3.2 mg/mL and 3.5 μL of concentrated sample immediately applied to a Quantifoil R2/1 300 copper mesh grid (Quantifoil Micro Tools GmbH), which had been glow discharged in a GloQube Plus glow discharger for 45 seconds at 25 mA and coated with graphene oxide (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... 4 µL of cell suspension was applied on 200-mesh R ¼ SiO2-film covered gold grids or 200-mesh R2/1 carbon-film covered copper grids (Quantifoil Micro Tools) (for P ...
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bioRxiv - Microbiology 2023Quote: ... 3µL of each sample was was pipetted onto a freshly glow-discharged Quantifoil R2/1 grid (Quantifoil Micro Tools GmbH, Großlöbicha, Germany), then blotted to remove excess sample and plunge-frozen into a mixture of liquid ethane and propane cooled in a bath of liquid nitrogen using a Vitrobot Mk 4 cryo-plunger (41) ...
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bioRxiv - Biochemistry 2022Quote: ... Protein-DNA complexes were applied to ultra-gold foil grids with holey gold support and 300 mesh size (R 0.6/1,Quantifoil Micro Tools GmbH); grids were flash-frozen as described [21] and were stored in liquid nitrogen until use.
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bioRxiv - Biochemistry 2024Quote: Samples were prepared by applying the polymerized R-β-Actin actin to freshly glow-discharged Quantifoil 3.5/1 on 200 mesh copper grids (Quantifoil GmbH, Germany), using a Leica GP2 (Leica Microsystems GmbH ...
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bioRxiv - Biochemistry 2022Quote: ... An aliquot of 3.5 μl of sample was placed on the glow-discharged cryo-EM grid (Quantifoil R1.2/1.3 or Quantifoil R2/1), blotted and plunge-frozen in liquid ethane using a Mark VI Vitrobot instrument maintained at 100% humidity with blot force 20 and blot time of 3 seconds ...
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bioRxiv - Molecular Biology 2021Quote: ... DRD1-SKF81297-LY3154207-miniGs-Nb35 complex at the concentration of 13 mg mL−1 were applied individually onto glow-discharged holey carbon grids (Quantifoil, Au200 R1.2/1.3) in a Vitrobot chamber (FEI Vitrobot Mark IV) ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL of the sample was applied to a glow-discharged R2/1 300 mesh holey carbon copper grid (Quantifoil Micro Tools GmbH) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...