Labshake search
Citations for Quantifoil Micro Tools :
501 - 550 of 730 citations for 7 Chloro 1 3 dihydro 5 phenyl 2H 1 4 benzodiazepin 2 thione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... 3 μL of the mixture was applied to a glow-discharge holey carbon grids (Quantifoil, Cu 200 mesh, R1.2/1.3). The grid was blotted for 4.5 s in 100% humidity before being flash frozen in liquid ethane by using a Vitrobot Mark IV (Thermo Fisher) ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... 3 μl of the samples were applied to glow-discharged holey carbon EM grids (Quantifoil 300 copper mesh, R1.2/1.3) in an EM-GP2 plunge freezer (Leica) ...
-
bioRxiv - Immunology 2023Quote: ... 3 μL of each LNP sample was deposited onto a holey carbon grid that was glow-discharged (Quantifoil R1.2/1.3) and vitrificated using a Vitrobot Mark IV System (FEI/Thermo Scientific ...
-
bioRxiv - Immunology 2023Quote: ... 3 μL of the purified complex were loaded onto a glow-discharged holey grid (Quantifoil Au 300 mesh R1.2/1.3), and subsequently were plunge-frozen in liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... of purified proteins of SARS-CoV2-ST or SARS-CoV2-STN were loaded onto glow-discharged (60 s at 20 mA) holey carbon Quantifoil grids (R2/1, 200 mesh, Quantifoil Micro Tools) using a Vitrobot Mark IV (ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2019Quote: Cells of strain Pla133T were mixed with the same volume of BSA-stabilized 15 nm colloidal gold solution (Aurion) and placed on holey carbon-coated 200 mesh copper grids (R2/1, Quantifoil, Jena, Germany) immediately before thin-film vitrification by plunge-freezing in liquid propane (63%)/ethane (37% ...
-
bioRxiv - Biochemistry 2022Quote: ... Protein-DNA complexes were applied to ultra-gold foil grids with holey gold support and 300 mesh size (R 0.6/1,Quantifoil Micro Tools GmbH); grids were flash-frozen as described [21] and were stored in liquid nitrogen until use.
-
bioRxiv - Cell Biology 2023Quote: ... 4 µL of cell suspension was applied on 200-mesh R ¼ SiO2-film covered gold grids or 200-mesh R2/1 carbon-film covered copper grids (Quantifoil Micro Tools) (for P ...
-
bioRxiv - Microbiology 2023Quote: ... 3µL of each sample was was pipetted onto a freshly glow-discharged Quantifoil R2/1 grid (Quantifoil Micro Tools GmbH, Großlöbicha, Germany), then blotted to remove excess sample and plunge-frozen into a mixture of liquid ethane and propane cooled in a bath of liquid nitrogen using a Vitrobot Mk 4 cryo-plunger (41) ...
-
bioRxiv - Biochemistry 2024Quote: ... Peak fractions were concentrated to ∼3.2 mg/mL and 3.5 μL of concentrated sample immediately applied to a Quantifoil R2/1 300 copper mesh grid (Quantifoil Micro Tools GmbH), which had been glow discharged in a GloQube Plus glow discharger for 45 seconds at 25 mA and coated with graphene oxide (Sigma Aldrich ...
-
bioRxiv - Biophysics 2019Quote: Samples for cryo-EM were prepared by applying 4 µl of purified TRPA1 to 1.2/1.3 holey carbon grids (Quantifoil) and blotting for 8-12 sec ...
-
bioRxiv - Biophysics 2021Quote: ... 4 µL of aldolase sample were applied on UltrAuFoil R1.2/1.3 300-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly plasma cleaned for 6 seconds at 15 Watts (75% nitrogen/25% oxygen atmosphere ...
-
bioRxiv - Biophysics 2021Quote: ... 4 µL of apoferritin sample were applied on UltrAuFoil R1.2/1.3 300-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly plasma cleaned for 7 seconds at 15 Watts (75% nitrogen/25% oxygen atmosphere ...
-
bioRxiv - Biophysics 2021Quote: The LlCsm RNP and RNP-target RNA samples at approximately 0.5 mg/ml were separately applied in 4 μL volume onto glow-discharged UltrAuFoil 300 mesh R1.2/1.3 grids (Quantifoil), blotted for 3 s at 88% humidity and flash-frozen in liquid ethane using FEI Vitrobot Mark IV ...
-
bioRxiv - Molecular Biology 2022Quote: ... for 15 min before 4 μL of sample was applied to a graphene oxide coated 1.2/1.3 UltrAuFoil grids (Quantifoil) at 4°C under 100% humidity86 ...
-
bioRxiv - Microbiology 2022Quote: ... Aliquots of 4 μL samples were applied to glow-discharged holey carbon girds (Cu, R1.2/1.3, 300 mesh, Quantifoil). The grids were blotted with force 2 for 3 seconds and plunged into liquid ethane using a Vitrobot (FEI Thermo Fisher) ...
-
bioRxiv - Molecular Biology 2024Quote: ... The peak fraction was then diluted with buffer N to 0.8 mg ml-1 and cryoEM grids prepared by applying 4 µl of the sample to glow-discharged Cu R1.2/1.3 300-mesh holey carbon grids (Quantifoil). Grids were prepared blotted at 8 °C and 90% humidity and plunged into liquid ethane using a Leica EM GP2.
-
bioRxiv - Biochemistry 2020Quote: Quantifoil R2/2 300 mesh holey carbon copper grids (Quantifoil Micro Tools, Großlöbichau, Germany) were glow-discharged (ELMO Glow Discharge System ...
-
bioRxiv - Cell Biology 2022Quote: MEF were seeded on R2/2 holey carbon on gold grids (Quantifoil or Protochips) coated with fibronectin in a glass bottom dish (Mattek or Ibidi ...
-
bioRxiv - Cell Biology 2022Quote: ... The cells were seeded on EM grids (Quantifoil SiO2 R2/2, 200 mesh, gold) in a 3D printed specimen holder [43] and grown overnight.
-
bioRxiv - Biochemistry 2023Quote: ... Quantifoil R2/2 Cu 400 mesh holey carbon grids (Quantifoil Micro Tools, Jena, Germany) coated with a 0.4 mg/ml graphene oxide suspension (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2023Quote: ... Quantifoil R1.2/1.3 400-square mesh Cu grids with 2 nm continuous carbon (Quantifoil) were glow-discharged for 8 s at 10 mA using a PELCO easiGlow glow discharging system (Ted Pella ...
-
bioRxiv - Biochemistry 2024Quote: ... Three microliters of sample were applied to glow-discharged R2/2 carbon grids (Quantifoil), which were blored for 5 s and at a blot force of 25 and plunge-frozen in liquid ethane with a Vitrobot Mark IV (ThermoFisher ...
-
A distinctive ligand recognition mechanism by the human vasoactive intestinal polypeptide receptor 2bioRxiv - Molecular Biology 2021Quote: The purified PACAP27–VIP2R–Gs complex (3 μL at 3.7 mg per mL) was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3). Vitrification was performed using a Vitrobot Mark IV (ThermoFisher Scientific ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: Samples (3 µL) were applied to a glow-discharged UltrAuFoil R1.2/1.3 Au 300 gold foil grids (Quantifoil GmbH, Großlöbichau, Germany) and were flash frozen in liquid ethane using a Vitrobot mark IV (Thermo Fisher Scientific ...
-
Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... 3 µL samples were applied to freshly glow-discharged Cu 200 Holey Carbon R1.2/1.3 grids (Quantifoil, for samples without FFosC), or Cu 200 Holey Carbon R2/1 grids (Quantifoil ...
-
bioRxiv - Biochemistry 2021Quote: The purified TIP39–PTH2R–Gs complex (3 μL at 8.5 mg per mL) was applied on a glow-discharged holey carbon grid (Quantifoil R1.2/1.3). Vitrification was performed using a Vitrobot Mark IV (ThermoFisher Scientific ...
-
bioRxiv - Molecular Biology 2022Quote: ... The complex (3 μL) was quickly applied on a glow-discharged UltraAuFoil R1.2/1.3 300 mesh holey Au grids (Quantifoil Micro Tools GmbH), blotted with Vitrobot Mark IV (FEI) ...
-
bioRxiv - Biochemistry 2022Quote: ... stock solution was mixed with 3.5 µL of the KWOCA 4 sample and 3 µl were immediately loaded onto the UltrAuFoil R 1.2/1.3 grids (Au, 300-mesh; Quantifoil Micro Tools GmbH). Prior to sample loading the grids were subjected to Ar/O2 plasma cleaning for 10 s on a Solarus 950 plasma cleaner (Gatan) ...
-
bioRxiv - Neuroscience 2023Quote: ... each time was sequentially blotted 3 times to 300-mesh holey carbon Au R1.2/1.3 grids treated with 25s of glow discharge (Quantifoil, Micro Tools GmbH). Fluorinated Fos-choline-8 (0.65 mM ...
-
bioRxiv - Biochemistry 2024Quote: ... Cryo-EM grids were prepared by applying 3 µl of concentrated sample onto 400-mesh R1.2/1.3 UltrAuFoil grids (Quantifoil Micro Tools GmbH), which had rendered hydrophilic by glow discharging at 15mA for 60seconds with a PELCO EasyGlow device (TED PELLA ...
-
bioRxiv - Biochemistry 2022Quote: ... An aliquot of 3.5 μl of sample was placed on the glow-discharged cryo-EM grid (Quantifoil R1.2/1.3 or Quantifoil R2/1), blotted and plunge-frozen in liquid ethane using a Mark VI Vitrobot instrument maintained at 100% humidity with blot force 20 and blot time of 3 seconds ...
-
bioRxiv - Molecular Biology 2021Quote: ... DRD1-SKF81297-LY3154207-miniGs-Nb35 complex at the concentration of 13 mg mL−1 were applied individually onto glow-discharged holey carbon grids (Quantifoil, Au200 R1.2/1.3) in a Vitrobot chamber (FEI Vitrobot Mark IV) ...
-
bioRxiv - Biochemistry 2022Quote: Three microliters of Ct80S-RAC pull-out sample at 200 nM concentration was applied on holey carbon grids (Quantifoil R2/1 grid, Quantifoil Micro Tools, GmbH) and plunged-frozen into liquid ethane using a Vitrobot (FEI) ...
-
bioRxiv - Microbiology 2020Quote: Usually 5 μL of liquid culture (OD680 ~ 0.3) was pipetted onto holey carbon-coated copper grids (R2/1, 200 mesh, Quantifoil Micro Tools, Jena, Germany). The grids were blotted from the reverse side and immediately plunged into the liquid ethane at liquid nitrogen temperature atmosphere using a Vitrobot ...
-
bioRxiv - Microbiology 2020Quote: ... 3.5 microliters of the purified virus sample was pipetted onto a glow-discharged R2/1 Quantifoil grid (Quantifoil Micro Tools GmbH, Jena, Germany), blotted for 2.5 seconds ...
-
bioRxiv - Microbiology 2022Quote: ... 5 µl of bacteria diluted to OD = 0.15 in PBS were deposited on a glow-discharged Quantifoil R2/1 holey carbon grids (Quantifoil Micro Tools GmbH, Germany) and 3 µl gold particles (10 nm BSA tracer ...
-
bioRxiv - Biochemistry 2022Quote: ... three microliters of concentrated phage particles were added to a freshly glow-discharged Quantifoil R2/1 grid (Quantifoil Micro Tools GmbH, Großlöbicha, Germany) and plunge-frozen with a Vitrobot Mk IV into a 50:50 mixture of liquid ethane:propane52
-
bioRxiv - Microbiology 2022Quote: Three-microliter aliquots of all AdSDV capsid populations (~1 mg/mL) were applied to glow-discharged Quantifoil holey carbon grids with a thin layer of carbon (Quantifoil Micro Tools GmbH) and vitrified using a Vitrobot Mark IV (FEI ...
-
bioRxiv - Microbiology 2023Quote: 3 µL of the purified FL or truncated RSV NC sample were applied to a glow-discharged R2/1 300 mesh holey carbon copper grid (Quantifoil Micro Tools GmbH) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
-
bioRxiv - Immunology 2021Quote: ... by shaking at 1200 rpm for 5 minutes at 37°C using a plate shaker (Quantifoil Instruments) after stimulating with collagen at a range of concentrations ...
-
bioRxiv - Cell Biology 2022Quote: Vero-E6 were seeded on holey carbon film R 5/20 gold 200 mesh EM grids (Quantifoil Micro Tools GmbH ...
-
bioRxiv - Microbiology 2022Quote: ... 2.7 μl of freshly purified sample was applied onto glow-discharged (30 s, 5 mA) grids (Quantifoil R 0.6/1 300 mesh Cu or Ultrafoil 0.6/1 300 mesh Au ...
-
bioRxiv - Biochemistry 2023Quote: 3.5 μL of LRRK2RCKW:E11 complex was applied to glow-discharged UltraFoil 300 mesh R1.2/1.3 grids (Quantifoil) and incubated in a FEI Vitrobot IV at 4°C and 95% humidity for 20 sec ...
-
bioRxiv - Biochemistry 2020Quote: ... KBP was diluted to 0.3 mg/ml in KBP dilution buffer and 4 μl were applied to glow-discharged 1.2/1.3 AuFoil gold grids (Quantifoil®). For the third approach ...
-
bioRxiv - Molecular Biology 2019Quote: The concentration of the core BBSome as derived from chemical crosslinking was adjusted to 0.05-0.08 mg/ml and 4 μl of sample was immediately applied to a glow-discharged UltrAuFoil® R1.2/1.3 holey gold grid (Quantifoil). After two minutes incubation at 13 °C and 100% relative humidity (RH) ...
-
bioRxiv - Microbiology 2022Quote: ... A 4□μl volume of SsoCsm complex was applied to holey carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh) covered by a graphene oxide layer (27) ...
-
bioRxiv - Molecular Biology 2021Quote: ... and applied onto freshly glow-discharged 200 square mesh Ultrafoil R2/2 gold grids (Quantifoil). The samples were plunge-frozen with a FEI Vitrobot Mark IV (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2021Quote: ... Freshly glow-discharged finder gold quantifoil grids (200 mesh R2/2 – Quantifoil MicroTools GmbH, Germany) were sterilized in 70% EtOH and placed in a glass-bottomed MatTek dish (MatTek Corporation ...
-
bioRxiv - Microbiology 2021Quote: ... were applied to freshly washed and glow-discharged R2/2 holey carbon copper grids (Quantifoil). The grids were manually back blotted for 2 to 3s with filter paper ...