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Citations for Quantifoil Micro Tools :
501 - 550 of 784 citations for 4 Bromo 3' 1 3 dioxolan 2 yl 2 fluorobenzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Primary cultured neurons were seeded onto holey gold EM grids (Quantifoil R 1/4 gold 200 mesh). The fiducial marker solution was prepared as for neocortical synaptosomes ...
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bioRxiv - Cell Biology 2024Quote: ... Quantifoil Holey SiO2 R 1/4 Au 200 mesh EM grids (Quantifoil Micro Tools #N1-s13nAi20-01) were glow discharged on both sides in a Pelco easyglow for 45 s at 0.37 mBar and 15 mA ...
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bioRxiv - Biophysics 2022Quote: Purified wt or mutant D263A or F55A MVMp capsid samples (5 μl) were applied onto R2/2 300 mesh copper grids (Quantifoil Micro Tools, Germany) and vitrified using a Leica EM CPC cryofixation unit ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 μL of urease samples at 1.3 mg/mL with 1 mM BME or with 10 mM SHA were applied to freshly glow-discharged Quantifoil R2/2 holey carbon grids (Quantifoil Micro Tools, Germany). The grids were blotted for 4 seconds ...
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bioRxiv - Biochemistry 2022Quote: ... and an aliquot of 2 μl of this sample was applied onto a plasma cleaned holey carbon grid (Quantifoil, R1.2/1.3, 200 mesh). The grid was blotted with Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: A 5 µl cell suspension containing 10 nm colloidal gold particles were deposited on the copper EM grids with holey carbon supported film (Quantifoil, 200mesh, R1/2). The EM grid were blotted with filter paper (Waterman ...
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bioRxiv - Cell Biology 2022Quote: ... A 3 µl volume of the Kif5B suspension was first deposited at the surface of a glow-discharged holey carbon grid (Quantifoil R2/2, Cu200) in the temperature (23 °C ...
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bioRxiv - Cell Biology 2024Quote: ... intact MVKCg1 yeasts cells resuspended in PBS were vitrified on Quantifoil Au R2/2 London Finder 300-mesh grids (Quantifoil Micro Tools GmbH) following a similar vitrification procedure as described above for crude membranes ...
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bioRxiv - Cell Biology 2024Quote: ... An aliquot of 3.5 μl of the crude membrane sample was deposited onto freshly glow-discharged Quantifoil R2/2 200-mesh grids (Quantifoil Micro Tools GmbH,) using the Leica EM GP (Leica Microsystems ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Grids were prepared by applying 3.5 μLs of the Z7757 complex or 2 μLs of the Lisuride complex to glow discharged UltrAuFoil holey gold grids (Quantifoil, Au300-R1.2/1.3). The girds containing Z7757 were then vitrified by plunge freezing into a 60/40 mixure of liquid ethane/propane using a Vitrobot mark IV (FEI ...
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bioRxiv - Cell Biology 2024Quote: ... An aliquot of 3.5 μl of sample was applied to freshly glow-discharged copper Quantifoil R2/2 200-mesh London Finder grids (Quantifoil Micro Tools GmbH) and then plunge frozen using a Leica EM GP plunger (Leica Microsystems ...
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bioRxiv - Biochemistry 2021Quote: ... 3 microlitres of undiluted eluate and of eluate which was diluted 1:2 or 1:4 in buffer A200 were blotted onto holey carbon R 1.2/1.3 copper 300 mesh grids (Quantifoil) and vitrified in liquid ethane using a Vitrobot MarkIV device (Thermofisher) ...
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bioRxiv - Biochemistry 2024Quote: ... 4 uL of the concentrated sample was applied to freshly glow-discharged R2/1 holey carbon grids (Quantifoil), blotted with blot force of 5 for 5 s using a Vitrobot Mark IV (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: 4 µl of the cultured yeast cells (OD600 of 0.4) were added to a glow-discharged (30 seconds at -30 mA) copper R2/2 holey carbon grid (Quantifoil Micro Tools GmbH, Jena, Germany), then the grid was rapidly plunge frozen in liquid ethane using a homemade plunge freezer and stored in liquid nitrogen until used ...
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bioRxiv - Biochemistry 2020Quote: ... 2.5 μL aliquots of purified Dnf1p–Lem3p or Dnf2p–Lem3p at a concentration of about 1.5 mg/mL were placed on glow-discharged holey carbon grids (Quantifoil Au R2/2, 300 mesh) and were flash-frozen in liquid ethane using a FEI Vitrobot Mark IV ...
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bioRxiv - Cell Biology 2022Quote: ... 4 µL of the mixture were pipetted onto a copper EM grid with holey carbon film (R2/2, 200 mesh, Quantifoil Micro Tools GmbH, Q43486) that had been freshly glow-discharged for 30 s at -30 mAmp ...
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bioRxiv - Biophysics 2024Quote: A 3 μL of aliquot Msmeg MmpL3 ΔC NDs at a concentration of 0.6 mg/mL was applied to glow-discharged (Quantifoil Cu R2/2, 300 mesh) holey carbon grids for 30 sec and blotted for 2×5 sec at 4°C and 100% humidity ...
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bioRxiv - Molecular Biology 2021Quote: Purified mtLSU or 55S mitoribosome samples (4 μL) were applied to freshly glow discharged R 3.5/1 holey carbon grids (Quantifoil) that were precoated with a 2-3 nm carbon layer using a Leica EM ACE600 coater ...
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bioRxiv - Cell Biology 2024Quote: ... 3.5 µl of 4 mg/mL proteasomal complexes was applied to glow-discharged R0.6/1 UltrAuFoil 300 mesh grids (Quantifoil) and frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: RNA granule preparations were diluted with RNA Granule buffer to an optical density A260 = 2 U/mL aliquots of the dilution deposited on glow-discharged Quantifoil grids (Quantifoil 1.2/1.3, 300 mesh copper) pre-coated with a continuous layer of 3 nm carbon ...
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bioRxiv - Molecular Biology 2022Quote: 3 μL of ~120 nM mitoribosome was applied onto a glow-discharged (20 mA for 30 sec) holey-carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ~3 nm thickness ...
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bioRxiv - Biophysics 2020Quote: ... 2 μL of a 1 in 6 dilution of the batch crystallisation was applied to the carbon side of glow discharged R2/2 Quantifoil™ grids (Quantifoil Micro Tools, Jena, Germany). Excess liquid was removed by blotting for 4-6 s with a Vitrobot (Thermo Fisher ...
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bioRxiv - Biochemistry 2022Quote: ... The endogenous samples were applied onto grids containing an additional thin layer of continuous carbon support (Quantifoil R1.2/1.3 Cu 200 mesh + 2 nm C) and incubated for 30 sec before blotting and freezing ...
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bioRxiv - Biochemistry 2024Quote: ... or calpain-3-titin/I81-I83 (2mg/mL) from Superose 6 increase 10/300GL column chromatography was loaded onto glow-discharged holey carbon grids (Quantifoil 1.2/1.3 + 2 nm Carbon). The grids were blotted with filter paper for 2.5 secs at 22 °C and then immediately plunged into liquid ethane cooled by liquid nitrogen using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: 3 μL of ∼120 nM mitoribosome was applied onto a glow-discharged (20 mA for 30 sec) holey- carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ∼3 nm thickness ...
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bioRxiv - Biophysics 2023Quote: ... 4 μl of the extracellular parasites were pipetted onto a glow-discharged (30 seconds at −30 mA) copper R2/2 holey carbon grid (Quantifoil Micro Tools GmbH, Jena, Germany). Samples were back-blotted with a Whatman filter paper (grade 1 ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μL of ∼120 nM mitoribosome was applied onto a glow-discharged (20 mA for 30 sec) holey-carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ∼3 nm thickness ...
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bioRxiv - Microbiology 2024Quote: ... The co-culture-colloidal gold bead mixture was added to glow-discharged copper R2/2 Quantifoil holey carbon grids (Quantifoil Micro Tools GmbH, Jena, Germany). Grids were blotted (under 100% humidity conditions ...
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bioRxiv - Biophysics 2021Quote: ... Log phase cultures were centrifuged at 2000 g for 2 minutes to concentrate the cells 10 times and 4 μl was directly applied to a glow-discharged holey carbon R2/1 Cu 200 mesh grid (Quantifoil). Plunge-freezing was performed using a VitroBot Mark 4 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... trehalose solution to mitigate crystalline ice formation and 4 μL of this mixture immediately applied to a R2/1 Cu 200 grid (Quantifoil), which had been glow-discharged for 1 min at 0.19 mbar and 20 mA in a PELCO easiGlow device shortly before use ...
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bioRxiv - Biophysics 2023Quote: ... 4 µL fresh sample (0.48 mg/mL) was applied on glow-discharged Au holey carbon grids (Quantifoil R 0.6/1) and the following operation were the same as describe above for preparing the sample of gMCM8/9 complex.
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bioRxiv - Cell Biology 2024Quote: Cells were seeded and grown overnight on Quantifoil carbon film gold grids (Quantifoil R 1/4 on 200 gold mesh) coated with 10 µg/ml FN ...
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bioRxiv - Biophysics 2024Quote: ... Microtubules (4 µl drop of 40 µM dilution) were placed onto a glow-discharged holey carbon grid (R2/1; Quantifoil) prepared on a JEC-3000FC (JEOL) ...
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bioRxiv - Cell Biology 2024Quote: ... A total of 4 µL of cell suspension (1500–2000 cells/µL) were applied to holey carbon R2/1 or R1/4 copper TEM grids (Quantifoil), and cells were plunge frozen on a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: The samples were frozen on copper or gold grids with holey carbon film coated with a 2 nm continuous carbon film (Quantifoil R1.2/1.3 ultrathin carbon, 300 mesh) freshly glow discharged for 20 s at 15 mA (PELCO easiGlow) ...
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bioRxiv - Neuroscience 2023Quote: ... 3.5 μL of CopiaGag capsid protein sample was applied to a 300-mesh copper grid with continuous 2 nm carbon film (Electron Microscopy Sciences, QUANTIFOIL® C2-C15nCu30-50) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
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bioRxiv - Molecular Biology 2021Quote: ... cells in suspension (4 μl of ~1,000 cells per μl) were blotted onto R2/1 carbon-coated 200-mesh copper grids (Quantifoil Micro Tools) and plunge frozen in a liquid ethane/propane mixture ...
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bioRxiv - Molecular Biology 2024Quote: ... about 0.9 mg/ml for DSS1–INTAC–PEC complex) was applied onto a glow-discharged holey carbon grid (Quantifoil Au, R1.2/1.3 or R2/2, 300 mesh). After blotting for 3 to 6 s ...
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bioRxiv - Cell Biology 2024Quote: ... the sample was gently resuspended and 4 µl was transferred to a glow-discharged holey carbon R2/1 copper grid (Quantifoil Micro Tools) suspended in the chamber of a Vitrobot (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: Freshly prepared ribosome–Sec61–KZR-8445 complex at 300–500 nM concentration was applied on glow discharged holey-carbon grids (Quantifoil, R1.2/1.3 with a 2-nm carbon film) and flash frozen with an automated plunge freezer (Leica microsystems) ...
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bioRxiv - Cell Biology 2020Quote: ... or holey SiO2 R1/4 TEM grids (Quantifoil) to allow for more robust correlation and cells were plunge frozen on a Vitrobot Mark IV (FEI ...
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bioRxiv - Cell Biology 2022Quote: ... Grids (200 Mesh Cu SiO2 R1/4, Quantifoil) were plasma cleaned for 30 s before plunging ...
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bioRxiv - Molecular Biology 2023Quote: ... a 4 µl sample of purified complex was applied to glow-discharged R2/1 300 mesh holey carbon copper grids (Quantifoil Micro Tools GmbH), using a conc ...
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bioRxiv - Cell Biology 2023Quote: 4 ul of Nannochloropsis suspensions (4 x 107 cells mL−1) were pipetted onto a 300 mesh gold Quantifoil grid with 2um holes (Quantifoil Micro Tools, GmbH) and manually blotted from the opposite side in a Vitrobot Mark IV (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... We glow-discharge-treated 200 mesh R1/4 or R2/4 Quantifoil holey carbon gold grids (Quantifoil®, Grossloebichau/Jena Germany) in a Pelco easiGlow (Ted Pella ...
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bioRxiv - Biochemistry 2022Quote: 4 µl aliquots of purified samples at A280 = 1 were applied onto glow-discharged holey carbon-coated grids (Quantifoil 300 mesh, Au R1.2/1.3), then adsorbed for 10 s ...
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bioRxiv - Cell Biology 2024Quote: ... and then 4 μL of the sample was applied to the glow-discharged R1/4 Carbon 200-mesh gold EM grid (Quantifoil Micro Tools). The EM grid was incubated in the chamber of Vitrobot (Vitrobot Mark 4 ...
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bioRxiv - Biophysics 2021Quote: Gold EM grids with R1/4 holey SiO2 film (Quantifoil) were glow-discharged in a EasiGlow (Pelco ...
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bioRxiv - Biophysics 2023Quote: 200 mesh Au grids with R1/4 SiO2 foil (Quantifoil) were glow-discharged for 90 sec at 0.38 mBar and 15 mA using a Pelco easiGlow device ...
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bioRxiv - Neuroscience 2022Quote: ... Gold Quantifoil grids (R1/4, Au 200 mesh, Quantifoil Micro Tools) were additionally coated with a 20-25 nm carbon layer (MED 020 ...