Labshake search
Citations for Quantifoil Micro Tools :
251 - 300 of 784 citations for 4 Bromo 3' 1 3 dioxolan 2 yl 2 fluorobenzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... 3 ul sample was applied onto glow-discharged R1.2/1.3 300 mesh UltrAuFoil gold grids (Quantifoil) (Russo and Passmore ...
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bioRxiv - Biophysics 2022Quote: Cryo-EM grids were prepared by applying 3 μL of sample (at the native 1 nM concentration) on glow discharged holey gold grids (Quantifoil Cu 1.2/1.3 400 mesh). Excess sample was removed by blotting with filter paper for 4 sec prior to plunge-freezing in liquid ethane using a FEI Vitrobot Mark IV at 100% humidity and 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: Cryo-EM grids were prepared by applying 3 µL sample (at a protein concentration of 1 mg/ml) on glow-discharged holey gold grids (Quantifoil Au 1.2/1.3 300 mesh). Excess sample was removed by blotting with filter paper for 2-3 s before plunge-freezing in liquid ethane (cooled to −181 °C ...
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bioRxiv - Cell Biology 2024Quote: Vitrified grids were prepared by applying 3 µl of 1 uM complex sample to a freshly glow- discharged holey carbon grids (Quantifoil R1.2/1.3 Au 200 mesh). The sample was blotted for 3-5 s at 4 ℃ with 100% humidity and plunged-frozen in liquid nitrogen cooled ethane (FEI TM ...
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bioRxiv - Biochemistry 2020Quote: ... 3 μL of the concentrated RdRp-RNA complex were mixed with 0.5 μl of octyl ß-D-glucopyranoside (0.003% final concentration) and applied to freshly glow discharged R 2/1 holey carbon grids (Quantifoil). The grids were blotted for 5 seconds using a Vitrobot MarkIV (Thermo Fischer Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... 3 µL of the concentrated RdRp-RNA complex were mixed with 0.5 µl of octyl ß-D-glucopyranoside (0.003% final concentration) and applied to freshly glow discharged R 2/1 holey carbon grids (Quantifoil). The grids were blotted for 7 seconds with blot force 5 using a Vitrobot MarkIV (Thermo Fischer Scientific ...
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bioRxiv - Biophysics 2020Quote: ... 3 μL of the concentrated RdRp-RNA complex were mixed with 0.5 μl of octyl ß-D-glucopyranoside (0.003% final concentration) and applied to freshly glow discharged R 2/1 holey carbon grids (Quantifoil). Prior to flash freezing in liquid ethane ...
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bioRxiv - Molecular Biology 2020Quote: ... a 2.2-µL aliquot of this sample was applied to a plasma cleaned holey carbon grid (R2/1, 200 mesh; Quantifoil) or Graphene Oxide-Lacey Carbon grid (300 mesh ...
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bioRxiv - Biophysics 2020Quote: ... Mycoplasma pneumoniae strain M129(ATCC 29342)cells were grown on 200 mesh gold grids coated with a holey carbon support (R 2/1, Quantifoil). Cells were cultivated at 37 °C in modified Hayflick medium ...
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bioRxiv - Microbiology 2022Quote: ... and 4 µl of the mixture was applied to a glow-discharged holey-carbon copper EM grid (R2/1 or R2/2, Quantifoil). The grid was automatically blotted from the backside for 4-6 s in a Mark IV Vitrobot by using a Teflon sheet on the front pad ...
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bioRxiv - Microbiology 2022Quote: ... mixed with 10 nm protein A-coated colloidal gold (Aurion) and applied onto a glow-discharged EM grid (200 mesh, R 2/1, Quantifoil) prior to plunge-freezing with a Leica EM GP2 automatic plunge-freezer.
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bioRxiv - Cell Biology 2023Quote: Cells were blotted on EM grids (R 2/1 holey carbon 200 films on Cu 200 mesh, Quantifoil Micro Tools). The grids were first glow-discharged and 4 µl of trypanosome wild-type culture (2-4 · 107 cells/ml ...
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bioRxiv - Microbiology 2023Quote: ... and 4 µl of the mixture was applied to a glow-discharged holey-carbon copper EM grid (R2/1 or R2/2, Quantifoil). The grid was automatically backside blotted for 4-6s in a Mark IV Vitrobot (Thermo Fischer Scientific ...
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bioRxiv - Biophysics 2023Quote: ... HeLa cells were trypsinized and seeded at a density of 0.5 – 0.7 X 105 cells/mL on carbon-coated gold Quantifoil grid (200 mesh, R 2/1, holey SiO2 film; Quantifoil Micro Tools GmbH ...
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bioRxiv - Microbiology 2024Quote: ... and 4 µl of the mixture was applied to a glow-discharged holey-carbon copper EM grid (R2/1 or R2/2, [Quantifoil]). The grid was automatically blotted from the backside for 4-6 s in a Mark IV Vitrobot by using a Teflon sheet on the front pad ...
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bioRxiv - Immunology 2023Quote: ... respectively) and 3 μl were kept 5 min at RT until their application onto glow-discharged holey carbon grids (Quantifoil, Au 300 mesh, R 0.6/1). The grids were blotted and then plunged into liquid ethane using a FEI Vitrobot Mark IV at 20°C and 95% relative humidity ...
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bioRxiv - Biophysics 2020Quote: Quantifoil grids (200 mesh Au R2/2 and Au Finder; Quantifoil Micro Tools GmbH ...
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bioRxiv - Microbiology 2022Quote: ... EM grids (R2/2, Cu 200 mesh; Quantifoil Micro Tools GmbH) were glow-discharged for 45 sec at 25 mA by PELCO easiGlow discharger ...
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bioRxiv - Molecular Biology 2021Quote: ... UltrAuFoil (Russo and Passmore, 2014) R2/2 200 mesh grids (Quantifoil) were glow discharged for 15 s at 30 mA in a Quorum SC7620 ...
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bioRxiv - Biochemistry 2023Quote: ... Holey carbon R2/2 300 mesh grids (Quantifoil Micro Tools GmbH) were glow-discharged for 90 sec at 25 mA using a PELCO easiGlow glow discharge cleaning system ...
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bioRxiv - Microbiology 2022Quote: ... Gold quantifoil R2/2 200 mesh grids (Quantifoil Micro Tools GmbH) were sterilised with 70% ethanol and placed in glass bottomed MatTek dishes (MatTek Corporation ...
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bioRxiv - Microbiology 2024Quote: Cryo-EM gold grids (Quantifoil R2/2 Au 200 mesh, Quantifoil) were placed in the slots of a custom-made PDMS stencil (Alvéole ...
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bioRxiv - Microbiology 2024Quote: Cryo-EM gold grids (Quantifoil R2/2 Au 200 mesh, Quantifoil) were placed in the slots of a custom-made PDMS stencil (Alvéole ...
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bioRxiv - Biochemistry 2024Quote: Quantifiol R 1.2/1.3 Au 400 mesh 2 nm Carbon (Quantifoil) were glow discharged at 0.2 atm for 30 s and were loaded onto a FEI vitrobot (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2020Quote: 3 μL of sample was applied onto a holey carbon grid (Quantifoil, Cu, R1.2/1.3, 300 mesh) rendered hydrophilic by a 30 s glow-discharge in air (11 mA current with PIB-10) ...
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bioRxiv - Plant Biology 2021Quote: Concentrated PSII solution (3 µl) was applied on glow-discharged holey carbon grids (Cu Quantifoil R1.2/1.3) that were vitrified for cryo-EM structural determination using a Vitrobot FEI (3 s blot at 4°C and 100% humidity) ...
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bioRxiv - Molecular Biology 2022Quote: ... The sample (3 μL) was applied to freshly glow-discharged Au 300 mesh R1.2/1.3 grids (Quantifoil) in a Vitrobot Mark IV (FEI ...
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bioRxiv - Biophysics 2021Quote: ... 3 μL was applied to Ultrafoil grids (R1.2/1.3, Au/Au, 300 mesh, Quantifoil Micro Tools GmbH) that had been glow-discharged for 2 min using a Pelco easiGlow (Ted Pella ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Samples (3 µL) were applied to glow-discharged Quantifoil R1.2/1.3 Cu/Rh 200 mesh grids (Quantifoil) (M4R-Gi1-Ipx and M4R-Gi1-Ipx-LY298 ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μl diluted protein sample was put on freshly glow-discharged Quantifoil R1.2/1.3 Cu grid (Quantifoil Micro Tools GmbH ...
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bioRxiv - Biophysics 2023Quote: ... 3 µL sample was applied to a freshly glow-discharged UltrAuFoil R1.2/1.3 300 mesh grids (Quantifoil), blotted with Whatman No ...
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bioRxiv - Biochemistry 2024Quote: Ternary complex sEGFR-Ex3HL/LH-CD3γε (3 µL) was applied to Quantifoil R1.2/1.3 Cu girds (Quantifoil) that had been glow discharged at 10 mA for 40 seconds with a sputter coater (JEOL JEC-3000FC) ...
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bioRxiv - Biophysics 2024Quote: ... 3 μL were adsorbed to glow-discharged holey carbon-coated grids (Quantifoil 300 mesh, Cu R1.2/1.3). were then blotted for 3 to 6 s at 100% humidity (4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... 3 μL of alpha synuclein fibrils were applied to the glow-discharged holey carbon Cu grids (Quantifoil) and blotted with filter paper using Vitrobot Mark IV (FEI Company) ...
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bioRxiv - Neuroscience 2024Quote: ... Supernatant after centrifugation mixed with 10-nm gold beads solution (Aurion, cat # 25486) at 1:1 ratio was added to the mounted grid (Quantifoil R2/2 Cu 200 EM). No other chemicals or stimulation were applied prior to plunge freezing ...
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bioRxiv - Plant Biology 2024Quote: 4 μL AtEDS1-AtPAD4-AtADR1 complex purified from Nb leaves was applied to glow-discharged Quantifoil holey carbon grids (Quantifoil Cu R1.2/1.3+2 nm C, 300 mesh). The protein was concentrated to 0.9 mg/mL prior to freezing ...
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bioRxiv - Biochemistry 2024Quote: 4 μl of the inhibition reaction mixture was applied onto plasma cleaned holey carbon grids coated with 2nm thick amorphous carbon (Quantifoil R 2/2, UT, 300 Mesh, Copper). Following 30 sec of incubation inside the chamber of FEI Vitrobot MK IV (FEI ...
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bioRxiv - Biochemistry 2024Quote: ... Those nucleosomes that passed the quality control were then loaded into a freshly glow discharged holey carbon golden grid (Quantifoil™ R 2/2 on 300 gold mesh) (2.5 µg of nucleosome diluted in 40 mM NaCl reconstitution buffer in 3 μl was loaded in the grid) ...
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bioRxiv - Microbiology 2021Quote: ... 3 μl of each sample was dispensed on Quantifoil R1.2/1.3 200-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly glow discharged for 30 seconds at 20 mA using a PELCO easyGLow™ Glow Discharge Cleaning System (Tedpella) ...
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bioRxiv - Immunology 2022Quote: ... 3 μl of sample was then dispensed on Quantifoil R1.2/1.3 200-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly glow discharged for 30 s at 20 mA ...
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bioRxiv - Biochemistry 2022Quote: ... 3 µl of TIR-SAVED complex was applied to Holey Carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh), glow discharged for 45 s at current of 45 mA in a EMITECH K100X glow discharger ...
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bioRxiv - Molecular Biology 2020Quote: A 3 μL sample solution was applied to a Quantifoil holey carbon grid R1.2/1.3 Mo 200 mesh (Quantifoil Micro Tools GmbH ...
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bioRxiv - Neuroscience 2021Quote: ... Aliquots of 3 μl were applied to glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3, 300 mesh) and plunge-frozen in liquid ethane using a Thermo Fisher Vitrobot Mark IV ...
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bioRxiv - Biochemistry 2021Quote: ... A 3-μl aliquot was pipetted on a holey carbon cryo-EM grid (1.3/1.2 Cu 200 mesh, Quantifoil) and vitrified by plunge-freezing in liquid ethane (Vitrobot Mark IV ...
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bioRxiv - Immunology 2021Quote: ... 3 μl of sample was then dispensed on Quantifoil R1.2/1.3 200-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly glow discharged for 30 s at 20 mA ...
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bioRxiv - Biophysics 2021Quote: ... 3 μl sample was applied to each side of the UltrAuFoil grid (300 mesh, R 1.2/1.3, Quantifoil), which had been glow discharged on both sides (25 mA ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 µl of DELE WT CTD sample at 3 mg/ml was applied onto 300 mesh R1.2/1.3 UltraAuFoil holey gold grids (Quantifoil) that were plasma cleaned for 15 s using Pelco glow discharge cleaning system (Ted Pella ...
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bioRxiv - Biophysics 2022Quote: ... A total of 3 µl of the solution was applied to freshly glow discharged R2.2 Cu300 grids (Quantifoil), blotted from the back side for 1.2s at 20°C and 99% humidity and immediately plunge frozen in liquid ethane using a Leica EM GP ...
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bioRxiv - Immunology 2023Quote: ... 3 μL of the purified complex (8 mg/ml) was deposited onto a glow-discharged holey grid (Quantifoil Au 300 mesh R1.2/1.3 ...
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bioRxiv - Biochemistry 2023Quote: ... A sample volume of 3 μL sample was applied to a freshly glow-discharged UltrAuFoil R1.2/1.3 300 mesh (Quantifoil); the sample was incubated for 1min and blotted with Whatman No ...