Labshake search
Citations for Quantifoil Micro Tools :
451 - 500 of 677 citations for trans 2 2 4 Methoxyphenyl 2 oxoethyl cyclohexane 1 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: Neutrophils were stimulated with 100 nM LTB4 in mHBSS for 15 mins at 37 °C in 35 mm glass bottom dishes containing glow-discharged Au SiO2 R1/4 200 mesh grids (Quantifoil GmbH) precoated with 25 μg/ml fibrinogen in DPBS ...
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bioRxiv - Immunology 2024Quote: ... a 4 μL aliquot of protein complex was applied to glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3, 300-mesh) or graphene oxide (GO)-coated holey-carbon gold grids (EMR ...
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bioRxiv - Bioengineering 2022Quote: ... holey carbon grids (Quantifoil R2/1 grid: Cu 300mesh, M2951C-1-300) were hydrophilized by a JEC-3000FC Auto Fine Coater (JEOL ...
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bioRxiv - Molecular Biology 2021Quote: ... bremelanotide– or THIQ–bound MC4R–Gs complexes at ∼ 4 mg/mL were applied onto glow-discharged holey carbon grids (Quantifoil R1.2/1.3). Excess samples were blotted away and the grids were vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFischer Scientific).
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bioRxiv - Genomics 2020Quote: ... Vitrified specimens were prepared by adding 4 μl disome samples at the concentration of ~ 150 nM to a glow-discharged holey carbon grid (Quantifoil R1.2/1.3), which was covered with a freshly made thin carbon film ...
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bioRxiv - Cell Biology 2020Quote: ... The MEFs were applied onto glow-discharged EM grids with a silicon-oxide support film (R1/4, Au mesh; Quantifoil, Jena, Germany). After incubation ...
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bioRxiv - Cell Biology 2022Quote: The cryo-EM grid was prepared by applying 3 μl of the protein samples (4 – 6 mg/ml) to glow-discharged Quantifoil R1.2/1.3 300-mesh gold holey carbon grids (Quantifoil, Micro Tools GmbH, Germany). Grids were blotted for 4.0 seconds under 100% humidity at 4°C before being plunged into the liquid ethane using a Mark IV Vitrobot (FEI) ...
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bioRxiv - Cell Biology 2023Quote: ... Four µL of the cells were subsequently deposited onto Quantifoil R2/4 200 mesh copper grids (Quantifoil Micro Tools GmbH, Jena, Germany), which were then manually blotted from the back with Whatman® Grade 1 filter paper for approximately 3 – 5 seconds ...
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bioRxiv - Biophysics 2023Quote: ... 4 μl suspended cells were loaded onto the front side of glow-discharged holy-carbon copper grids (Quantifoil R1.2/1.3, 200 mesh), and 2 μl medium was subsequently added to the back side of the grids ...
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bioRxiv - Biochemistry 2021Quote: 3.5 µl protein solution containing 0.05 µM CUL4-NEDD8/ROC1/DDB1/DCAF1-CtD/Vprmus/SAMHD1 complex and 0.25 µM UBCH5C-ubiquitin conjugate (S4 A, B Fig) were applied to a 300 mesh Quantifoil R2/4 Cu/Rh holey carbon grid (Quantifoil Micro Tools GmbH) coated with an additional thin carbon film as sample support and stained with 2% uranyl acetate for initial characterisation ...
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bioRxiv - Biochemistry 2022Quote: ... Spike trimer - Fab complexes (4 μl) were applied to the pre-glow-discharged holey carbon grids (Quantifoil grid, Cu 300 mesh, R1.2/1.3). The grids were then blotted for 2 seconds with filter paper in 100% relative humidity and 8 °C and plunged into the liquid ethane to freeze samples using FEI Vitrobot system (FEI).
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bioRxiv - Molecular Biology 2022Quote: ... An aliquot of 4 μL protein sample of Spike protein was applied onto a glow-discharged 300 mesh grid (Quantifoil Au R1.2/1.3) and GraFuture™-RGO grid which was supported with a thin layer of RGO (reduced graphene oxide) ...
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bioRxiv - Biochemistry 2023Quote: 4 μL of Fcα–J–FcRL4 (0.3 mg/mL) were applied to glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3, 300 mesh) or Ni-Ti grids (CryoMatrix ...
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bioRxiv - Molecular Biology 2021Quote: ... Cu R2/1 grids (Quantifoil) were plasma cleaned using a NanoClean plasma cleaner (Fischione Instruments ...
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bioRxiv - Microbiology 2024Quote: ... cupper R2/1 grids (Quantifoil) were glow discharged ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 μl of protein sample (about 1.5 mg/ml) was applied onto a glow-discharged holey carbon grid (Quantifoil Au, R1.2/1.3, 300 mesh). At a temperature of 4°C and under a humidity of 100% ...
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bioRxiv - Biophysics 2020Quote: ... An aliquot of 4 μl pY39 α-syn fibril solution was applied to a glow-discharged carbon electron microscope grid (Quantifoil R1.2/1.3, 300 mesh). After 10 s of incubation ...
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bioRxiv - Molecular Biology 2021Quote: The purified GIP1-42–GIPR–Gs–Nb35 complex at a concentration of 6-7 mg/mL was mixed with 100 μM GIP1-42 at 4 C and applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, Au 300 mesh) that were subsequently vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: The purified tirzepatide–GIPR–Gs–Nb35 complex at a concentration of 18-20 mg/mL was mixed with 100 μM tirzepatide at 4°C and applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, Au 300 mesh) that were subsequently vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: The purified non-acylated tirzepatide–GIPR–mini-Gs–Nb35 complex at a concentration of 14-16 mg/mL was mixed with 100 μM non-acylated tirzepatide at 4°C and applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, Au 300 mesh) that were subsequently vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: The purified peptide 20–GIPR–Gs–Nb35 complex at a concentration of 5-6 mg/mL was mixed with 100 μM peptide 20 at 4°C and applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, Au 300 mesh) that were subsequently vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: The purified peptide 20–GCGR–Gs–Nb35 complex at a concentration of 12-14 mg/mL was mixed with 100 μM peptide 20 at 4°C and applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, Au 300 mesh) that were subsequently vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... Four microliters of purified Cx36 proteins (4-6 mg/ml) were applied onto a negatively glow-discharged holey carbon grid (Quantifoil R1.2/1.3 Cu 300 mesh). For samples involving Cx36-WT ...
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bioRxiv - Biochemistry 2022Quote: ... R3.5/1 Cu 200 grids (Quantifoil) were glow-discharged on a glass clover slide for 60 seconds at 25 mA and plunging into liquid ethane was performed using an EM GP (Leica Microsystems) ...
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bioRxiv - Neuroscience 2020Quote: Holey carbon grids (Quantifoil R2/1) covered with either 2 nm amorphous carbon (Quantifoil ...
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bioRxiv - Cell Biology 2023Quote: Quantifoil R3.5/1 Au200 grids (Quantifoil) were glow discharged for 30 s in an Auto306 Edwards Turbo Coater at power 6 (20 mA ...
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bioRxiv - Biochemistry 2024Quote: ... Grids (Quantifoil UltrAuFoil R 0.6/1) were glow-discharged (20 mA ...
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bioRxiv - Biophysics 2020Quote: ... The WT and Y222F MTs were applied to lacey carbon grids (Agar Scientific AGS166-4 400 mesh copper grids) and holey carbon grids (Quantifoil R1.2/1.3 300 mesh copper grids) immediately after application of glow discharge to the grids ...
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bioRxiv - Biophysics 2023Quote: ... Micropatterns covered an area of 8 × 8 squares around the center of the cryo-TEM grids (Quantifoil R1/4 or R1/20, Au 200 mesh, SiO2 film) as previously described31 ...
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bioRxiv - Biochemistry 2021Quote: UltrAuFoil 0.6/1 300 mesh grids (Quantifoil) were glow discharged in residual air at 25 mA for 3 min using a Pelco EasiGLOWTM ...
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bioRxiv - Biochemistry 2022Quote: UltrAuFoil® gold grids (0.6/1, Quantifoil) (Russo and Passmore ...
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bioRxiv - Biochemistry 2023Quote: ... UltrAuFoil 0.6/1 300-mesh grids (Quantifoil) were glow discharged in residual air at 20LmA for 60 s each side using a Pelco EasiGLOW ...
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bioRxiv - Biochemistry 2021Quote: ... were applied to glow discharged (2.2×10-1 mbar for 20 sec) Quantifoil holey carbon grids (R2/1, 200 mesh, Quantifoil). The grids were plunge vitrified into a liquid ethane/propane mix using a Vitrobot Mark IV at 4 °C and 95% humidity ...
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bioRxiv - Biochemistry 2020Quote: ... Quantifoil 300 mesh gold grids (either 0.6/1 carbon film, 0.6/1 or 1.2/1.3 UltrAUfoil, Quantifoil Micro Tools GMBH) were glow discharged for 30 seconds prior to use ...
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bioRxiv - Biochemistry 2024Quote: ... 3 μL of the crosslinked sample at 1 mg/mL was applied to glow-discharged 0.6/1 UltrAuFoil 300 mesh grids (Quantifoil) and frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: 3 μL of purified PSI complex at 1 mg Chl mL−1 were applied to glow-discharged (GloQube Quorum, 40 sec, 20mA) holey carbon grids (Quantifoil 300 Au R1.2/R1.3 ...
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bioRxiv - Biophysics 2020Quote: Holey carbon grids (Quantifoil R2/1, 200 mesh) were plasma treated using a Solarus plasma cleaner (Gatan) ...
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bioRxiv - Plant Biology 2021Quote: Holey carbon grids (Quantifoil R2/1, 200 mesh) were plasma cleaned using a Solarus plasma cleaner (Gatan) ...
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bioRxiv - Molecular Biology 2020Quote: ... transferred onto allgold UltrAuFoil R0.6/1 grids (QuantiFoil), and partially hydrogenated following a previously described procedure [17] ...
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bioRxiv - Molecular Biology 2022Quote: ... The no carbon sample (Quantifoil Cu200 R2/1) was blotted for 1.5 s from the back side right after sample application and immediately plunge frozen in liquid ethane ...
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bioRxiv - Molecular Biology 2022Quote: R3.5/1 Holey carbon copper grids (QUANTIFOIL, Germany) were glow-discharged by UV light ...
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bioRxiv - Cell Biology 2023Quote: ... 200 mesh R2/1 Au EM grids (Quantifoil) were coated with laminin (1:10 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... Cu supported 3.5/1 Holey Carbon grids (Quantifoil) were glow discharged on both sides for six seconds each using atmospheric air in order to render them more hydrophilic ...
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bioRxiv - Cell Biology 2024Quote: ... Cu supported 3.5/1 Holey Carbon grids (Quantifoil) were glow discharged on both sides for six seconds each using atmospheric air to render them more hydrophilic ...
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bioRxiv - Biochemistry 2024Quote: ... 0.6/1-mm holey gold grid (Quantifoil UltrAuFoil). After a 30 second incubation ...
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bioRxiv - Cell Biology 2024Quote: ... EM grids (Quantifoil R3.5/1 Au 200 mesh) were glow-discharged using a plasma cleaner (PLASMAFLO PDC-FMG-2 ...
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bioRxiv - Biochemistry 2024Quote: ... 0.6/1-mm holey gold grid (Quantifoil UltrAuFoil). After a 30 s incubation ...
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bioRxiv - Biophysics 2022Quote: A 3 µl aliquot of freshly concentrated or thawed protein at ∼1 mg/ml was deposited on an UltraAuFoil 300 mesh R 0.6/1 grid (Quantifoil Inc., UK) at 4 °C and 100% humidity in a Vitrobot Mark IV robot (Thermo Fisher) ...
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bioRxiv - Pathology 2023Quote: ... bombycis spores (1×108 spores/mL) were placed on grids (Cu R2/1, 200 mesh) (Quantifoil Micro Tools GmbH, Jena, Germany) for 5 min and germinated in 0.1 M KOH for 15 min at 30°C ...
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bioRxiv - Biochemistry 2023Quote: 4 µL of protein solution (total protein concentration = 1 mg/mL) were applied to freshly glow-discharged QUANTIFOIL® R2/1 300 mesh grids (Quantifoil Micro Tools GmbH ...