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Citations for Quantifoil Micro Tools :
451 - 500 of 719 citations for 1 2 Chloropyridin 3 yl 3 3 dimethylazetidin 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: A 3 µL aliquot of purified CDTbD623A/D734Aprotein sample at a concentration of 30 µM was applied to a glow discharged QUANTIFOIL® copper R 1.2/1.3 grid with a 2 nm thin extra carbon layer (QUANTIFOIL, GMBH). For calcium depleted CDTb (28 µM) ...
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bioRxiv - Biophysics 2023Quote: ... 3 or 3.5 μl of LRRK2RCKW:inhibitor:E11 DARPin complex were applied to a glow-discharged UltraAuFoil Holey Gold 200 mesh R2/2 grid (Quantifoil, Catalog # Q250AR2A) and incubated in a FEI Vitrobot IV chamber at 4°C and 95% humidity for 20 sec ...
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bioRxiv - Biochemistry 2023Quote: 2.4 µL of sample were applied to a freshly glow discharged 200-mesh Cu + 2 nm C R1.2/1.3 grid (Quantifoil Micro Tools), blotted for 6 s at 100% humidity ...
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bioRxiv - Biochemistry 2022Quote: ... 2–2.5 µl of protein solution was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3 Au 200 mesh). The endogenous samples were applied onto grids containing an additional thin layer of continuous carbon support (Quantifoil R1.2/1.3 Cu 200 mesh + 2 nm C ...
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bioRxiv - Cell Biology 2024Quote: HeLa WT cells or HeLa cells stably expressing Apaf1-GFP were plated on 200 mesh gold EM grids with a holey carbon film R2/2 (Quantifoil). HeLa WT cells were transfected with Apaf1-SNAP-tag ...
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bioRxiv - Microbiology 2024Quote: ... A 4.5 µL droplet of the culture was applied to glow discharged copper grids (Quantifoil R2/2, Quantifoil Micro Tools) and blotted for 2.5 s in a 95% humidity chamber at 20 °C ...
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bioRxiv - Microbiology 2024Quote: ... We initially prepared frozen-hydrated viruses deposited onto Quantifoil R2/2 or R1.2/1.3 grids (Quantifoil Micro tools GmbH, Germany) or glow discharged Lacey grid (Ted Pella Inc. ...
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bioRxiv - Microbiology 2024Quote: ... A 4.5 µL droplet of the culture was applied to glow discharged copper grids (Quantifoil R2/2, Quantifoil Micro Tools) and blotted for 2.5 s in a 95% humidity chamber at 20 °C ...
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bioRxiv - Biochemistry 2024Quote: ... was applied to glow-discharged (GloQube® Plus, Quorum, UK) Quantifoil R1.2/1.3 or R2/2 mesh 200 copper holey carbon grid (Quantifoil, Germany), blotted under 100% humidity at 4°C for 6–8 s ...
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bioRxiv - Microbiology 2024Quote: ... The mixture was added onto glow-discharged copper R2/2 Quantifoil holey carbon grids (Quantifoil Micro Tools GmbH, Jena, Germany). Grids were blotted for 6-8 seconds (under 100% humidity conditions ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µL of 10 µM 11-16Ub[K48]-GFP-35 was pipetted on a holey carbon R2/2 Cu200 grid (Quantifoil) that was plasma cleaned by a SCD005 sputter coater (Bal-Tec ...
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bioRxiv - Cell Biology 2024Quote: ... 3 µL of LRRK2RCKW:RN277:E11 DARPin complex were applied to a glow-discharged UltraAuFoil Holey Gold 200 mesh R2/2 grid (Quantifoil, Q250AR2A) and incubated in a FEI Vitrobot IV chamber at 4 °C and 95 % humidity for 20 sec ...
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bioRxiv - Biochemistry 2024Quote: HeLa cells stably expressing either HRS-mScarlet or HRS-mCherry were grown on 200 mesh gold EM grids with a holey carbon film R2/2 (Quantifoil). Cells were incubated with 50 ng/ml EGF- Alexa 647 ...
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bioRxiv - Plant Biology 2021Quote: ... 3 µl of suspended cells in culture medium were applied to the front side of glow-discharged R2/2 carbon-coated copper grids (Quantifoil MicroTools), with additional 1 µL cells added to the back side ...
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bioRxiv - Biochemistry 2021Quote: ... Three microliters of the protein solution were applied on a glow-discharged holey carbon grids (200 mesh Quantifoil R2/2 molybdenum), which had been treated with H2 and O2 mixtures in a Solarus plasma cleaner (Gatan ...
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bioRxiv - Biophysics 2023Quote: ... 3 to 3.5 μl of complex was applied to a glow-discharged UltrAuFoil Holey Gold 200 mesh R2/2 grid (Quantifoil, Catalog # Q250AR2A) and incubated in a FEI Vitrobot IV chamber at 4°C and 95% humidity for 20 sec ...
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bioRxiv - Cell Biology 2022Quote: ... The IL-11Δ10/IL-11RαD1-D3/gp130D1-D3 complex (0.5 mg/mL) was blotted onto UltrAuFoil grids (R2/2, Quantifoil Micro Tools GmbH) that had been subjected to glow discharge (15 mA for 30 s) ...
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bioRxiv - Biochemistry 2024Quote: ... 3 µL of suspended cells in culture medium were applied to the front side of glow-discharged R2/2 carbon-coated copper grids (Quantifoil MicroTools), with additional 1 µL cells added to the back side ...
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bioRxiv - Cancer Biology 2024Quote: ... or 0.5 mg/ml of RanGTP•selinexor-XPO1•ASB8ΔN16•ELOB/C supplemented with 2-fold excess ASB8ΔN16-ELOB/C and selinexor) was applied to a 400-mesh copper grid (Quantifoil R1.2/1.3). For unliganded XPO1 ...
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bioRxiv - Biophysics 2021Quote: 3 μl of the human sperm sample were applied to glow discharged copper R2/2 200-mesh holey carbon grid (Quantifoil Micro Tools) and plunge frozen in liquid ethane using an automatic plunge freezer (Vitrobot ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated at 37°C and 5% CO2 for 16h prior to trypsinization and seeding onto 200 mesh gold holey carbon grids (R2/2-2C; Quantifoil Micro Tools), which were placed in 3D printed grid holders [39] ...
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bioRxiv - Molecular Biology 2021Quote: ... After another 3 min at 37 °C the termination complexes were placed on ice for 15 min and subsequently applied to Quantifoil R2/2 holey carbon grids (Quantifoil Micro Tools) either coated with a thin continuous carbon film or with graphene oxide flakes (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... 4 µl of the solution was applied to a glow-discharged (30s at 35 mA) copper R2/2 holey carbon grid (Quantifoil Micro Tools). After removing excess liquid by blotting the grid from the back side with Whatman filter paper for 2 s ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were applied to freshly glow-discharged (Edwards S150B, 45 sec, 30-50 mA, 1,2kV, 10-2 mbar) copper holey carbon grids (Quantifoil, Cu-R1.2/1.3) under 100% humidity ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μl of the mixture was deposited on glow discharged R2/2 Copper 300 mesh holey carbon-coated support grids (Quantifoil, Jena, Germany) along with 1 μl of HEPES pH 10 and 1 μl of hydrogen peroxide 0.9 % ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: A 4 μL solution of co-isolated small particles were applied to glow-discharged copper grids (Quantifoil R2/2, Quantifoil Micro Tools), blotting for 3-seconds at 4 °C with 90% humidity and plunging into liquid ethane using a Lecia EM GP device (Leica Microsystems ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: A 4 μL solution of co-isolated small particles were applied to glow-discharged copper grids (Quantifoil R2/2, Quantifoil Micro Tools), blotting for 3-seconds at 4 °C with 90% humidity and plunging into liquid ethane using a Lecia EM GP device (Leica Microsystems ...
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bioRxiv - Neuroscience 2021Quote: ... 400 mesh Quantifoil R1.2/1.3 grids with empty holes and 400 mesh Quantifoil R1.2/1.3 grids coated with a 2 nm thin carbon layer (Quantifoil Micro Tools GmbH, Germany). The grids were rendered hydrophilic by glow discharge using a Pelco easiGlow glow discharger (Ted Pella Inc. ...
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bioRxiv - Microbiology 2020Quote: ... Cells were concentrated by centrifugation and 3 μL aliquots of the cell suspension are applied to glow-discharged R2/2 200 mesh copper Quanti-foil grids (Quantifoil Micro Tools), the sample was pre-blotted for 30 seconds ...
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bioRxiv - Cell Biology 2020Quote: ... A 5 µl drop of the chemically fixed cell culture was deposited on a glow-discharged Quantifoil 200 mesh R2/2 electron microscopy grid (Quantifoil, Großlöbichau, Germany) pre-coated with a gold bead solution ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µL aliquots of 0.1 mg/ml cross-linked ternary complexes were applied to freshly glow-discharged R2/2 Quantifoil 200 mesh copper grids (Quantifoil Micro Tools). Grids were blotted in a climate chamber set to 20 °C and at 95% humidity before plunge frozen in liquid ethane set at −183 °C using a Leica EM GP system (Leica Microsystems) ...
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Computational toolbox for ultrastructural quantitative analysis of filament networks in cryo-ET databioRxiv - Cell Biology 2021Quote: B16-F1 cells were cultured as described above and seeded onto 200 mesh gold holey carbon grids (R2/2-2C; Quantifoil Micro Tools). Prior to cell seeding ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μl of the resulting mixture was spotted onto glow-discharged copper EM grids (Quantifoil 300 Au mesh, R2/2 carbon film (Quantifoil Micro Tools). The grids were then blotted for 2 or 4 seconds (blot force −10 ...
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bioRxiv - Cell Biology 2024Quote: ... An aliquot of a 5 μl sample was applied to a glow-discharged holey carbon grid (Quantifoil R2/2, Au, 400 mesh). After waiting for 60 s ...
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Mycobacteria form viable cell wall-deficient cells that are undetectable by conventional diagnosticsbioRxiv - Microbiology 2022Quote: ... of which 3-4 µL was applied on glow-discharged 200 mesh copper grids with an extra thick R2/2 carbon film (Quantifoil Micro Tools). Vitrification was performed using an automated Leica EM GP plunge-freezer ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were applied to freshly glow-discharged (Edwards S150B, 45 s, 30−50 mA, 1.2 kV, 10−2 mbar) copper holey carbon grids (Quantifoil, Cu-R1.2/1.3) under 100% humidity at 4°C.
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bioRxiv - Biochemistry 2023Quote: 4.5 µL of freshly mixed Nup98 condensate and capsid mixture with protein A-gold (10 nm) were applied onto a glow-discharged Quantifoil R2/2 copper grid (Quantifoil Micro Tools). The grid was blotted in the front for 2.5 s at 15 °C with 90% relative humidity and then plunged into liquid ethane using a Lecia EM GP device (Lecia Microsystem) ...
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bioRxiv - Biophysics 2022Quote: Purified wt or mutant D263A or F55A MVMp capsid samples (5 μl) were applied onto R2/2 300 mesh copper grids (Quantifoil Micro Tools, Germany) and vitrified using a Leica EM CPC cryofixation unit ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 μL of urease samples at 1.3 mg/mL with 1 mM BME or with 10 mM SHA were applied to freshly glow-discharged Quantifoil R2/2 holey carbon grids (Quantifoil Micro Tools, Germany). The grids were blotted for 4 seconds ...
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bioRxiv - Cell Biology 2022Quote: ... A 3 µl volume of the Kif5B suspension was first deposited at the surface of a glow-discharged holey carbon grid (Quantifoil R2/2, Cu200) in the temperature (23 °C ...
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bioRxiv - Biochemistry 2022Quote: ... and an aliquot of 2 μl of this sample was applied onto a plasma cleaned holey carbon grid (Quantifoil, R1.2/1.3, 200 mesh). The grid was blotted with Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: A 5 µl cell suspension containing 10 nm colloidal gold particles were deposited on the copper EM grids with holey carbon supported film (Quantifoil, 200mesh, R1/2). The EM grid were blotted with filter paper (Waterman ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Grids were prepared by applying 3.5 μLs of the Z7757 complex or 2 μLs of the Lisuride complex to glow discharged UltrAuFoil holey gold grids (Quantifoil, Au300-R1.2/1.3). The girds containing Z7757 were then vitrified by plunge freezing into a 60/40 mixure of liquid ethane/propane using a Vitrobot mark IV (FEI ...
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bioRxiv - Cell Biology 2024Quote: ... intact MVKCg1 yeasts cells resuspended in PBS were vitrified on Quantifoil Au R2/2 London Finder 300-mesh grids (Quantifoil Micro Tools GmbH) following a similar vitrification procedure as described above for crude membranes ...
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bioRxiv - Cell Biology 2024Quote: ... An aliquot of 3.5 μl of the crude membrane sample was deposited onto freshly glow-discharged Quantifoil R2/2 200-mesh grids (Quantifoil Micro Tools GmbH,) using the Leica EM GP (Leica Microsystems ...
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bioRxiv - Cell Biology 2024Quote: ... An aliquot of 3.5 μl of sample was applied to freshly glow-discharged copper Quantifoil R2/2 200-mesh London Finder grids (Quantifoil Micro Tools GmbH) and then plunge frozen using a Leica EM GP plunger (Leica Microsystems ...
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bioRxiv - Cell Biology 2020Quote: 4 µl of the cultured yeast cells (OD600 of 0.4) were added to a glow-discharged (30 seconds at -30 mA) copper R2/2 holey carbon grid (Quantifoil Micro Tools GmbH, Jena, Germany), then the grid was rapidly plunge frozen in liquid ethane using a homemade plunge freezer and stored in liquid nitrogen until used ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 μl of protein sample (about 0.73 mg/ml) was applied onto a glow-discharged holey carbon grid (Quantifoil Au, R2/2, 300 mesh). After blotting for 3 s ...
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bioRxiv - Biochemistry 2020Quote: ... 2.5 μL aliquots of purified Dnf1p–Lem3p or Dnf2p–Lem3p at a concentration of about 1.5 mg/mL were placed on glow-discharged holey carbon grids (Quantifoil Au R2/2, 300 mesh) and were flash-frozen in liquid ethane using a FEI Vitrobot Mark IV ...
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bioRxiv - Cell Biology 2022Quote: ... 4 µL of the mixture were pipetted onto a copper EM grid with holey carbon film (R2/2, 200 mesh, Quantifoil Micro Tools GmbH, Q43486) that had been freshly glow-discharged for 30 s at -30 mAmp ...