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Citations for G-Biosciences :
301 - 321 of 321 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... Fixed cells were lysed in 3X volume of lysis buffer (1% SDS, 10mM EDTA, 50mM Tris-HCl pH 8.0, 1X protease inhibitor cocktail (G-Biosciences, 786-433). Cells were sonicated for 35 minutes using a Diagenode Bioruptor to shear chromatin to 200-500bp ...
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bioRxiv - Molecular Biology 2023Quote: ... at a ratio of 15:1 (see Supplementary Information, 2. for more details) and 10 µL silica magnetic beads (G-Biosciences, USA). The mixture was briefly vortexed and incubated for 20 minutes at room temperature while shaking at 1000 rpm to facilitate DNA binding ...
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bioRxiv - Biochemistry 2024Quote: Colonies showing evidence of both LacZ and His3 expression were cultured and lysed using Zymolyase solution (50:49:1, glycerol:Zymolyase [G-Biosciences]: 1M Tris) and prey plasmids were purified by alkaline lysis miniprep (Joly ...
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bioRxiv - Systems Biology 2024Quote: ... Supernatant lysate was collected and SmEn was purified in the gravity flow mode using Ni-NTA resin (G-bioscience, Cat. # 786-1547) following the manufacturer’s recommended procedure with slight modifications ...
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bioRxiv - Physiology 2021Quote: ... gels were rinsed in diH2O for 15 minutes and immersed in Coomassie stain (Lab Safe GEL Blue; G-Biosciences; St. Louis, MO, USA) for 2 hours ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the cytotoxicity of extracts and pure compounds was determined according to the manufacturer's instructions using the CytoScan LDH cytotoxicity assay (G-Biosciences, St. Louis, MO). Briefly ...
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bioRxiv - Genomics 2021Quote: ... and pellets frozen at -80°C. DNA was extracted from pellets (approx. 100 mg) using an OmniPrep kit (G-Biosciences, St. Louis, MO) in 1 ml lysis buffer (G-Biosciences ...
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bioRxiv - Genomics 2021Quote: ... filter cuttings contained in the 2 ml Lysing Matrix E tubes were submerged in 294 µl 10X Tris-EDTA (100 mM Tris, 10 mM EDTA, pH 8.0, G-Biosciences, Cat. No: 501035446) and 6 μl lysozyme solution (50 mg.ml-1 ...
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bioRxiv - Molecular Biology 2024Quote: ... Particle pellets were resuspended in 1× STE buffer (100 mM NaCl, 10 mM Tris pH 8.0, and 1 mM EDTA) (G-Biosciences, St. Louis, MO). The 293T/17 cells were collected and lysed with RIPA lysis buffer and clarified via centrifugation (1800 × g for 10 min) ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were then spun for 10 min at 6000 rpm at 4 °C and pre-cleared by incubating them under rotation with 25 μl of Sepharose CL-4B beads (G-Biosciences, #786-1560) for further 30 min at 4 °C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... stored in an envelope in a -80 freezer since collection) and dried, pinned specimens (i.e., traditional museum preservation method) using an OmniPrep Genomic DNA Extraction Kit (G-Biosciences, St. Louis, MO) and evaluated DNA quality using agarose gel electrophoresis and quantity using Qubit 2.0 fluorometer (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... MBP-fused proteins were purified using MBP resin and the GST-tagged proteins were purified with glutathione agarose beads (G-biosciences®; USA). For in vitro pull-down assays ...
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bioRxiv - Bioengineering 2020Quote: Actinobacillus succinogenes 130Z (ATCC 55618) was obtained from the American Type Culture Collection (Manassas, VA) and maintained in tryptic soy broth media (G-Biosciences, St. Louis, MO) with 10 g/L glucose (Fisher Chemical ...
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bioRxiv - Neuroscience 2023Quote: ... by homogenizing the tissue with a 2 cm3 glass-Teflon homogenizer at 800 rpm in lysis buffer (0.32 M sucrose, 5 mM Hepes, and 10 ml ddH2O) supplemented with 1:100 diluted proteases inhibitors (G-Biosciences, Cat. no: 786-433) and 1:100 diluted phosphatases inhibitors (Sigma-Adrich ...
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bioRxiv - Biochemistry 2022Quote: ... A final concentration of 2 mM CaCl2 was added to the protein eluate prior to incubation with Calmodulin agarose beads (G-Biosciences; Cat# 786-282) for 90 min at 4 °C ...
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bioRxiv - Bioengineering 2023Quote: ... Protein concentration of EV samples was determined using a bicinchoninic acid (BCA) assay using the manufacturer’s protocol (785-571; G-Biosciences, St. Louis, MO, USA). Equal amounts of protein of EV or lysate samples were then subjected to western blot analysis for ALIX (ab186429 ...
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bioRxiv - Biophysics 2024Quote: ... biotin was conjugated to the λ-phage DNA using either photobiotin (PHOTOPROBE®, Vector) or psoralen-biotin (HOOK™-Psoralen-PEO-Biotin, G-biosciences). The biotin reagent solution was added to the purified λ-phage DNA solution in a 50:1 volume ratio for photobiotin and a 100:1 for psoralen-biotin ...
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bioRxiv - Evolutionary Biology 2020Quote: Genomic DNA from molecular collection specimens was extracted from the thorax or leg(s) using OmniPrep Genomic DNA Extraction Kits (G-Biosciences, St. Louis, MO, USA) or DNeasy Blood and Tissue Kits (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: ... All surfaces for tissue collection and processing were sanitized using 70% ethanol and then treated with an RNAse inhibitor (RNAse out, G-Biosciences, St. Louis, MO, USA) to maintain integrity of isolated RNA ...
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bioRxiv - Cancer Biology 2024Quote: ... The reaction was allowed to proceed for 20 min at 56°C and alkylated for 30 min in the dark with 10 mM iodoacetamide at room temperature (G-Biosciences, St. Louis, MO, USA). Proteins were precipitated at –20°C with ethanol ...
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bioRxiv - Microbiology 2020Quote: ... and PDGF-D by enzyme-linked immunosorbent assay (ELISA, TGF-β: Thermo Fisher Scientific, Waltham, MA; CTGF and PDGF-D: G-Biosciences, St. Louis, MO; Abclonal, Woburn, MA) as previously reported and the data from these prior experiments was used in the current investigations.22 Each experiment was performed with two technical replicates.