Labshake search
Citations for G-Biosciences :
251 - 300 of 321 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2022Quote: Purification of tagged-GRFT was achieved by using a zinc-chelating resin (binding capacity 20mg/mL) (G-Biosciences). A calculated amount of resin was added to a 0.8 mL Pierce centrifuge spin column based on the estimated total protein yield obtained by Western blot analysis ...
-
bioRxiv - Molecular Biology 2024Quote: ... The cells were lysed with Nonidet P-40 (NP40) buffer supplemented with PIC (Cat: 786-437, G-Bioscience). Importin-ß was precipitated by protein A/G beads conjugated with anti-Importinβ-1 or anti-p24 antibody while incubating for 12 h in 4°C ...
-
bioRxiv - Biochemistry 2024Quote: ... 6 M Urea) and dialyzed against 100 volume TP70 Urea buffer in Tube-O-Dialyzer™(G-Biosciences) at 4 °C overnight and then dialyzed against 100 volume TP70 buffer (TP70 Urea buffer without Urea ...
-
bioRxiv - Neuroscience 2024Quote: ... Equal amounts of protein lysates were denatured in SDS-polyacrylamide gel electrophoresis (PAGE) Sample Loading Buffer (G-Biosciences), separated on 4-12% Bolt Bis-Tris Plus Protein Gels (ThermoFisher) ...
-
bioRxiv - Physiology 2024Quote: ... with protease and phosphatase inhibitor cocktails (97063-010, VWR and 786-450, G-Biosciences, St, Louis, MO, USA) using a THb Handheld Tissue Homogenizer ...
-
bioRxiv - Bioengineering 2024Quote: ... The resultant post-insertion mixture was purified using Sepharose CL-6B gel filtration columns (G-Biosciences, 76361-752).
-
bioRxiv - Cell Biology 2021Quote: ... Total protein concentration in BALF was determined using a Micro Bicinchoninic Acid Protein Assay kit (G-Biosciences; #786-572).
-
bioRxiv - Microbiology 2022Quote: His-FhaAMtb was induced with 1mM IPTG at 18°C overnight and purified on Ni-NTA resin (G-Biosciences, 786-940 in 5 ml Bio-Scale Mini Cartridges ...
-
bioRxiv - Cell Biology 2022Quote: ... The plates were incubated for two days at 30 □C before the X-Gal (#RC-212, G-Biosciences, USA) overlay ...
-
bioRxiv - Microbiology 2021Quote: ... Supernatants were collected and total protein was normalized using the Non-Interfering Protein Assay (G-Biosciences, St. Louis, MO).
-
bioRxiv - Bioengineering 2022Quote: ... T7 RNA polymerase promoter was used for the overexpression of mutant protein by IPTG (G-Biosciences, St. Louis, USA) induction ...
-
bioRxiv - Microbiology 2022Quote: Detection of SA-β-Gal activity was performed using a colorimetric senescence β-Galactosidase histochemical staining kit (G-Biosciences) or a fluorescent Cell Meter™ Cellular Senescence Activity Assay Kit with Xite™ Red beta-D-galactopyranoside (AAT Bioquest ...
-
bioRxiv - Physiology 2022Quote: ... and GH (following 6 hrs-food withdrawal) were measured using commercially available ELISA kits (TSH ELISA kit, G-Biosciences, Cat No ...
-
bioRxiv - Microbiology 2023Quote: ... Genomic DNA was prepared from liquid cultures using the XIT Genomic DNA from Gram-Positive Bacteria kit (G-Biosciences), as instructed by the manufacturer ...
-
bioRxiv - Genomics 2022Quote: Genomic DNA was extracted from 30 mg of urediniospores per isolate using the Omniprep DNA isolation kit (G-Biosciences). DNA concentration was determined using Qubit 3.0 Fluorometer (LifeTechnologies ...
-
bioRxiv - Cancer Biology 2023Quote: Whole cell lysates were prepared by dissolving (10 million cells per treatment) cell pellets in RIPA buffer (G-Bioscience,) complete with 1X Protease and Phosphatase inhibitor cocktail (Thermo Scientific ...
-
bioRxiv - Plant Biology 2024Quote: ... yeast-competent cells of Y2H GOLD strain were prepared according to manufacturer’s instructions (Fast™ Yeast Transformation, G-Biosciences). The two respective constructs were co-transformed in Y2H GOLD competent cells ...
-
bioRxiv - Cell Biology 2024Quote: Isolated brush border was homogenized at 4 °C for 30min in RIPA lysis buffer (G-Biosciences catalog #786-489) with cOmplete Protease Inhibitor Cocktail followed by centrifugation at 14,000g for 15 min ...
-
bioRxiv - Cell Biology 2020Quote: ... Seeds obtained from the T0 parental lines were sown on MS medium supplemented with 30 mg/L Kanamycin (G-Biosciences) for selection of successfully transformed transgenics ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... We assessed overall protein concentration in the upper fraction of the solution using RED 660™ protein assay (G-Biosciences) measured in triplicates and then diluted the sample to 0.45 mg/mL ...
-
bioRxiv - Cell Biology 2021Quote: ... and the protein concentration of the samples was measured by Micro Bicinchoninic Acid Protein Assay kit (G-Biosciences; #786-572). 2-mercaptoethanol was added to a final concentration of 1% (v/v) ...
-
bioRxiv - Microbiology 2021Quote: ... monolayers were overlaid with 2 ml of a solution consisting of equal parts of 1.4% (w/v) Low Melt Agarose (G-BioSciences) and 2X MEM (Gibco ...
-
An engineered receptor-binding domain improves the immunogenicity of multivalent SARS-CoV-2 vaccinesbioRxiv - Microbiology 2020Quote: ... supernatant pH was adjusted to 8.5 by addition of 1/20 volume 1M Tris pH=9.0 (G-Biosciences, 786-476), and the supernatant was filtered by 0.45 μm filter prior to purification on CaptureSelect™ C-tagXL Columns ...
-
bioRxiv - Cancer Biology 2021Quote: The F(ab′)2 fragments were generated using trastuzumab and the F(ab′)2 fragmentation kit following the manufacturer’s instructions (G-Biosciences).
-
bioRxiv - Biochemistry 2023Quote: ... The concentration of the protein was done using Bradford assay (Puregene PG-035-500mL) or BCA assay (G-Biosciences-786570). For western blotting 25 ug protein was loaded in each well ...
-
bioRxiv - Neuroscience 2023Quote: Media samples were collected from individual hCO wells and run using the Cytoscan LDH Cytotoxicity Assay (G-Biosciences, 786-210) per manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... frozen tumor tissues were crushed to powder and lysed with urea lysis buffer containing 8 M urea (G-Biosciences #BC89), 75 mM NaCl ...
-
bioRxiv - Genomics 2024Quote: High molecule weight (HMW) DNA of Ectocarpus male (Ec32) and female (Ec25) were isolated using OmniPrep™ kit (G-Biosciences) with slight modifications ...
-
bioRxiv - Cell Biology 2024Quote: Mitochondrial ferritin in HCEC-B4G12 and F35T cells were quantified using Immunotag™ Mitochondrial Ferritin ELISA kit (G-Biosciences, USA). Protein was extracted following the same protocol used for the cytosolic ferritin ELISA ...
-
bioRxiv - Genomics 2024Quote: ... we extracted DNA from a single adult female using a modified OmniPrep extraction protocol (G-Biosciences, St. Louis, Mo, USA); we followed the manufacturer’s protocol for solid tissue ...
-
bioRxiv - Bioengineering 2021Quote: ... were reacted for 2 h with 150 μL/cm2 of a 3 mg/mL suspension of sulfo-succinimidyl-4-(p-maleimidophenyl)-butyrate (S-SMPB, #BC24, G-Biosciences) in phosphate buffered saline solution (PBS ...
-
bioRxiv - Biochemistry 2021Quote: ... Cells were then sonicated and cell lysate containing GST tagged anti-GFP nanobody was incubated with glutathione resin (G-Biosciences, U.S.A.) at 4°C for 1 h ...
-
bioRxiv - Plant Biology 2020Quote: ... All Ulva gene and promoter sequences were cloned from genomic DNA, isolated via CTAB (Clarke, 2009) method or Omniprep for plant (G-Biosciences).
-
bioRxiv - Molecular Biology 2021Quote: ... Beads were washed three times in 1X TESH buffer with 0.15 M NaCl and resuspended in 40 µl of RIPA buffer (G-Biosciences, MO) on ice for 1 hour ...
-
bioRxiv - Microbiology 2022Quote: ... Particle pellets were resuspended in 1× STE buffer (100 mM NaCl, 10 mM Tris pH 8.0, and 1 mM EDTA) (G-Biosciences, MO). The 293T/17 cells were collected and lysed with RIPA lysis buffer and clarified via centrifugation (1800 x g for 10 min) ...
-
bioRxiv - Genomics 2022Quote: ... DNA was also extracted from 20 mg of isolate Pca203 spores using the Omniprep™ DNA isolation kit from G-Biosciences for library preparation with the Illumina TruSeq Nano DNA protocol and Illumina NovaSeq sequencing in the S2 flow cell at UMGC to produce 150 bp paired-end reads ...
-
bioRxiv - Physiology 2022Quote: ... Levels of TSH and GH were determined by enzyme-linked immunoassay (ELISA) using commercially available ELISA kits (TSH ELISA kit, G-Biosciences, Cat No ...
-
bioRxiv - Cell Biology 2020Quote: ... Cellular lysates were prepared by using lysis buffer (supplied in RnD Systems Proteome Profiler Human Phosphokinase Array Kit supplemented with 1X “Protease Arrest” and 2X “Phosphatase Arrest” (G-Biosciences)) ...
-
bioRxiv - Microbiology 2021Quote: ... sanguinis genomic DNA was prepared from overnight (O/N) liquid cultures using the kit XIT Genomic DNA from Gram-Positive Bacteria (G-Biosciences). Strain 2908 ...
-
bioRxiv - Biochemistry 2022Quote: ... The combined supernatants were dialyzed twice against 100 volumes of buffer M containing 1 M KCl (for 6 hours and overnight) using a 1000 DaMWCO Tube-O-Dialyzers (G-Biosciences). The concentration of TP 70 was determined by measuring A230 (1unit A230 = 240pmol TP70) ...
-
bioRxiv - Microbiology 2023Quote: ... transcripts were detected by gene-specific 32P-labeled oligonucleotides (Supplementary Table S1) in hybridization buffer (G-Biosciences, Saint Louis, MI, USA) and exposed to a phosphor screen overnight ...
-
bioRxiv - Biochemistry 2024Quote: ... The gel was fixed in 50% ethanol and 10% acetic acid for 30min and was stained with Coomassie Blue R250 (G-Biosciences). The portions of the gel containing the samples were excised and the gel pieces were subjected to in-gel trypsin digestion [30].
-
bioRxiv - Molecular Biology 2024Quote: ... All Ulva gene and promoter sequences were cloned from genomic DNA, isolated via CTAB (Clarke, 2009) or OmniprepTM for Plant (G-Biosciences).
-
bioRxiv - Synthetic Biology 2024Quote: ... The supernatant was transferred to a new tube and mixed with 2 mL of His-Binding/Wash Buffer (786-542, G-Biosciences) and 5 μL of HisTag Dynabeads (10104D ...
-
bioRxiv - Immunology 2021Quote: ... the cells were induced with IPTG overnight at 18°C followed by sonication and purification using Ni-NTA resin (Cat. No # 786-940, G-Biosciences, USA). The purity was confirmed by SDS-PAGE and the quantity was measured using NanoDrop 2000 (Thermo Fischer Scientific ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... we first added 200 µL hexane containing 300 ng/µL of each mbCHC in a 2-mL glass vial and then used a nitrogen evaporator (BT1603 G-Biosciences®) to evaporate the hexane with only mbCHCs precipitate at the bottom of the vial ...
-
bioRxiv - Biochemistry 2021Quote: ... The detergent present in the incubation mixture with BPS was removed using a DetergentOUT™ spin column (G-Biosciences, St Louis, MO). The protein was concentrated to 25-30 µM with the use of a Centrisart I MWCO 100 kDa concentrator (Sartorius AG ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 1.6 µL 1X TE Buffer (pH 8.0, Low EDTA (Tris-EDTA; 10 mM Tris base, 0.1 mM EDTA)) (PN 786-150, G-Biosciences, St. Louis, MO). Each dPCR reaction mix was then vortexed three times for five second pulses ...
-
bioRxiv - Cell Biology 2023Quote: ... The blots were blocked with 5% BSA in TBST and blotted according to manufacturer’s recommendations for the indicated primary and secondary antibodies (Table S2) followed by development with the femtoLUCENT™ PLUS-HRP Chemiluminescent kit (G-Biosciences) for the anti-GDF3 blot and the SuperSignal™ West Pico Chemiluminescent kit (Thermo Fisher ...
-
bioRxiv - Molecular Biology 2023Quote: ... Membranes were blocked for >60 min at room temperature with gentle shaking in 5% BLOT-QuickBlocker (w/v) (G-Bioscience 786-011) in phosphate buffered saline with 0.05% Tween-20 v/v (PBS-T).