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Citations for SouthernBiotech :
151 - 200 of 207 citations for Rat Orexin Receptor Type 2 HCRTR2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... wells were washed six times with PBS supplemented with 0.1 % Tween20 and incubated for 2 h with 1 μg/mL biotinylated goat α-mouse IgM or IgG1 (SouthernBiotech) at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... Antibody isotyping kit was purchased from Southern Biotech (Cat#5300-05). FITC-conjugated streptavidin was purchased from BD Biosciences (Cat#554060) ...
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bioRxiv - Microbiology 2020Quote: ... IgG-bound DNP-beads were next incubated with 1 µg/mL Alexa Fluor647-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech) in PBS-TH.
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bioRxiv - Microbiology 2020Quote: ... Antibody binding was detected using directly labeled IgG or Alexa Fluor647-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech). Samples were measured using flow cytometry (BD FACSVerse ...
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bioRxiv - Immunology 2019Quote: ... culture supernatants were incubated 2 h at 37°C on wells pre-coated with 5 μg/mL of goat-anti-mouse IgM (Southern Biotech) and saturated with PBS 2% BSA ...
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bioRxiv - Immunology 2021Quote: ... cells were resuspended in calcium-containing HBSS and incubated at room temperature for 5 minutes before activation by anti-IgM F(ab′)2 (Southern Biotech) or VLPs ...
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bioRxiv - Developmental Biology 2022Quote: ... They were washed in blocking buffer 2X for 2 hours and imaged in situ or mounted on microscope slides using Fluoromount-G (Southern Biotech). Immunostaining Analysis ...
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bioRxiv - Immunology 2022Quote: ... IgG bound to the rSARS-CoV-2 S protein was detected using a horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibody (Southern Biotech) incubated for 1 hour ± 10 minutes at 24°C ± 2°C ...
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bioRxiv - Immunology 2022Quote: ... a standard curve for mouse isotype was established for each plate by coating 2 columns with 50 μL goat anti-mouse Ig (1 μg/mL in PBS; Southern Biotech) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... plates were coated with 2 µg/mL of goat anti-human kappa or goat anti-human lambda antibodies cross-absorbed against mouse IgG (Southern Biotech) at 4°C overnight ...
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bioRxiv - Microbiology 2022Quote: ... bacteria were incubated with a Goat F(ab’)2 anti-human Kappa-Alexa Fluor 647 (pre-diluted in PBS 0.1%BSA; 2.5 μg/mL, Southern Biotech #2062-31) to detect bound Fab fragments ...
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bioRxiv - Immunology 2019Quote: ... the membrane was re-blocked with 5% BSA in 1x TBST for 2 hours at room temperature before probing with 1:5000 dilution of anti-human IgG-HRP (Southern Biotech) in 5% BSA/1x TBST for 1 hour at room temperature ...
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bioRxiv - Immunology 2020Quote: ... or IgG purified from Ggta1+/+ or Ggta1-/- mouse serum (60 µg/mL, PBS 2% BSA, wt/vol, 30 min.) followed by anti-mouse IgG-PE (Southern Biotech #1030-09 ...
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bioRxiv - Immunology 2020Quote: ... 500 ng of F(ab’)2 fragments of α-μ (clone JDC-15; Dako [α-human]; OB1022; Southern Biotech [α-mouse]) and α-CD19 (clone HD37 ...
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bioRxiv - Immunology 2020Quote: ... for detection of αGal and IgG purified from Ggta1+/+ or Ggta1-/- mouse serum (60 µg/mL, PBS 2% BSA, wt/vol, 30 min.) followed by anti-mouse IgG-PE (Southern Biotech #1030-09 ...
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bioRxiv - Microbiology 2021Quote: ... Fab fragments were detected with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech, 1:500). After washing ...
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bioRxiv - Neuroscience 2020Quote: ... Nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) with the use of DAPI Fluoromount-G (0100-20; Southern Biotech). Sections were also stained for β-galactosidase (β-gal ...
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bioRxiv - Immunology 2021Quote: ... B cells were then cultured for a further 2 days in RPMI (as described above) or supplemented with 500ng/ml Igκ (Southern Biotech). Where indicated the following reagents were added into in vitro cultures ...
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bioRxiv - Bioengineering 2021Quote: ... arrays were incubated for an additional 2 h at RT and 60 rpm with goat anti-mouse IgG conjugated to HRP (Southern Biotech) diluted 1:5000 in blocking solution ...
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bioRxiv - Microbiology 2020Quote: ... Guts were washed 5 times in PBS-T and then mounted on slides in Flouromount plus 4’,6-diamidino-2-phenylindole nuclear stain (DAPI, Southern Biotech) and sealed with a cover slip ...
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bioRxiv - Cell Biology 2020Quote: ... localization of antibody labeling was detected after a 1 hr incubation with secondary antibody diluted in PBS containing 2% normal goat serum and mounting under a coverslip with DAPI Fluoromount-G (Southern Biotech). PPCs were visualized by fluorescent microscopy and their numbers were assessed qualitatively by visual examination ...
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bioRxiv - Biochemistry 2020Quote: ... The immunoreactivity was detected by incubation with Rabbit F(ab’)2 Anti-Goat IgG(H+L)-HRP (6020-05, Southern Biotech) diluted 3000 × in PBS-T.
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed again and the nuclei were stained with DAPI (4’,6-diamidino-2-phenylindole) before mounting with Fluoromount-G (Southern Biotech). Primary antibodies against histone H3 methylation on lysine 4 ...
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bioRxiv - Cancer Biology 2024Quote: ... permeabilized with 0.1% Triton X-100 (Fisher Biotech, Hampton, NH, USA), and blocked with PBS supplemented with goat serum (2%, v/v) (Southern biotech) and 0.1% Tween-20 (Thermo Fisher) ...
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bioRxiv - Immunology 2024Quote: ... membranes were incubated with primary antibodies overnight at 4 °C and during 2 hours at room temperature with horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit IgG (SouthernBiotech, USA). Membranes were then revealed using G:BOX Chemi XL1.4 Fluorescent & Chemiluminescent Imaging System (Syngene ...
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bioRxiv - Immunology 2022Quote: ... kit following manufacturer’s protocol for intracellular staining of FITC IgA (Southern Biotech), IgG2b ...
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bioRxiv - Microbiology 2020Quote: ... the wells were incubated for 2 h at 37°C with HRP-mouse anti-human IgG antibody (dilution 1:2,000) (Southern Biotech, Birmingham, AL) followed by the peroxidase substrate ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips with stained cells were washed 2 x 5 min in PBS and then mounted with Fluoromount-G (Southern Biotech 0100-01) for image acquisition ...
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bioRxiv - Microbiology 2022Quote: ... the beads were incubated with 1 µg/mL Alexa Fluor647-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech, 2062-31) in PBS-TH for 30 min at 4 °C with shaking ...
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bioRxiv - Immunology 2022Quote: ... were coated overnight at 4°C with 2 μg/mL of monkey cross-adsorbed goat anti-human IgG (Southern Biotech #2049-01) diluted in phosphate-buffered saline (PBS) ...
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bioRxiv - Biochemistry 2022Quote: ... and then treated with alkaline phosphatase (AP)-conjugated goat anti-rabbit-IgG (Southern Biotech; 1:8000 in TBST with 2% (w/v) bovine serum albumin) ...
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bioRxiv - Physiology 2020Quote: ... The sections were processed for immunostaining of Pan-CK (--) as described above with 4’,6-diamidino-2-phenylindole (DAPI) containing Fluormount-G (SouthernBiotech, Birmingham, AL) to visualize nuclei.
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bioRxiv - Microbiology 2021Quote: ... samples were further statically incubated for 1 h at 4°C with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech, 1:300) and 6 μM Syto9 (Live/Dead BacLight Bacterial Viability Kit ...
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bioRxiv - Microbiology 2021Quote: ... secondary antibody at RT for 2h and stained with 4’,6-diamidino-2-phenylindole (DAPI) before mounting onto slides with Fluoromount-G (Southern Biotech, Birmingham, AL).
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bioRxiv - Molecular Biology 2020Quote: ... Worms were rinsed in PBS (3×10mins, 3 × 1hr) and equilibriated in mounting media with 4’,6-diamidino-2-phenylindole DAPI (Fluoromount G, Southern Biotech, Birmingham, AL) overnight before mounting and imaging.
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bioRxiv - Developmental Biology 2019Quote: ... A 1 μg/μl 4’6-diamidino-2-phenylindole (DAPI) solution was applied to the slides for nuclear staining prior to mounting in Fluoromount-G (Southern Biotech, 0100-01) with 34×60 mm coverslips (VWR ...
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bioRxiv - Immunology 2022Quote: ... cells were washed twice with FACS buffer and incubated for 30 min at 4°C with 1:1000 diluted PE-conjugated goat F(ab)’2 anti-human IgG (Southern Biotech 2042-09) in the dark ...
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bioRxiv - Immunology 2022Quote: ... ELISA plates were coated with 1 μg/ml recombinant SARS-CoV-2 S protein (43) and serially diluted serum samples were added and detected via anti-monkey IgG-HRP (Southern Biotech, Birmingham, AL). Plates were developed using a TMB substrate (source ...
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bioRxiv - Molecular Biology 2022Quote: ... Mycoplasma test was performed using Mycoplasma detection kit by Southern Biotech (13100-01), according to manufacturer’s instructions.
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bioRxiv - Biophysics 2021Quote: ... Cell lines were tested for mycoplasma contamination via the Mycoplasma Detection Kit (Southern Biotech). Transient transfections were performed with Lipofectamine 2000 (Life Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... and were regularly checked for mycoplasma contamination using a Mycoplasma Detection Kit (SouthernBiotech; 131001). Cells were transfected with 3 μg of DNA using FuGENE HD (Promega ...
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bioRxiv - Immunology 2020Quote: ... Total serum immunoglobulin levels were detected using SBA Clonotyping System HRP kit (Southern Biotech).
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bioRxiv - Immunology 2022Quote: ... Class-switched serum immunoglobulin levels were detected using SBA Clonotyping System HRP kit (Southern Biotech).
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bioRxiv - Evolutionary Biology 2021Quote: ... Plates were incubated with serially diluted serum or gut content (50 μL, PBS 1% BSA, wt/vol, 2 h, RT) and standard mouse IgA (Southern Biotech #0106-01, prepared in duplicates) and washed (5x ...
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bioRxiv - Immunology 2020Quote: ... Plates were incubated with serially diluted mouse sera (50 µL, PBS 1% BSA, wt/vol, 2 h, RT) and standard mouse IgG (Southern Biotech #0107-10, prepared in duplicates) and washed (5x ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by quantification of IgG1 abundance using the SBA Clonotyping System-HRP kit (SouthernBiotech; 5300-05) following the manufacturer’s instruction ...
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bioRxiv - Genomics 2023Quote: ... Cells were checked for mycoplasma contamination using the Mycoplasma detection kit (Southern Biotech, catalog #13100-01) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were regularly validated as mycoplasma free by a PCR-based mycoplasma detection kit (Southern Biotech).
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bioRxiv - Cell Biology 2020Quote: The apoptosis level of PC12 cells was determined by ApoScreen Annexin V kit (SouthernBiotech, Birmingham, AL, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Antibody subtype identification was performed using the SBA ClonotypingTM System/HRP Kit (Southern Biotech, Birmingham, AL, USA). To minimize the background impurities observed during immunoelectron microscopy ...