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Citations for SouthernBiotech :
51 - 100 of 207 citations for Rat Orexin Receptor Type 2 HCRTR2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... Plates were washed using ELISA wash buffer and incubated with HRP-conjugated anti-Ig secondary antibodies (Southern Biotech) for 2 hours in humid storage at 4°C ...
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bioRxiv - Immunology 2023Quote: ... ELISA antibodies for measuring total and HDM-specific Igs in serum were purchased from Southern Biotech (Birmingham, AL). ELISA data were analyzed using SoftMax Pro software (Molecular Devices).
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bioRxiv - Molecular Biology 2021Quote: ... followed by incubation with secondary antibody (anti-rat IgG HRP-conjugated, Southern Biotech, 1:10,000) for 1 h at room temperature ...
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bioRxiv - Immunology 2021Quote: ... followed by a Rat anti-mouse IgG2a -Alexa Fluor 647 detection antibody (SB84a, Southern Biotech). Cells stained with the detection antibody alone were used as an isotype control ...
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bioRxiv - Immunology 2019Quote: ... the same ELISA procedure as described above was performed except using anti-human IgA and anti-human IgA-HRP antibodies (SouthernBiotech, AL). Corresponding immunoglobulin isotypes were used as standards after serial dilutions.
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bioRxiv - Immunology 2023Quote: ... The concentration of Ig in serum supernatants was measured using a standard sandwich ELISA with anti-human Ig primary (Cat#: 2048-01, Southern Biotech) and secondary antibodies (Cat# ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies for immunoblotting were: horseradish peroxidase (HRP)-conjugated goat anti-rat IgG (Southern Biotech 3010-05), HRP-conjugated goat anti-mouse IgG (Southern Biotech 1010-05) ...
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bioRxiv - Immunology 2022Quote: ... plates were washed 4× with ELISA wash buffer and a 1:4000 dilution of goat anti-mouse IgG (SouthernBiotech, catalog #1030-05) in ELISA blocking buffer was added to each well and incubated at 37°C for 1 hour followed by 4 washes with ELISA wash buffer ...
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bioRxiv - Cell Biology 2020Quote: ... goat anti rabbit Ig (4010-05) and goat anti rat Ig HRP (horseradish peroxidase, 3010-05, Southern Biotech), goat anti human Ig (109-035-003 ...
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bioRxiv - Immunology 2021Quote: ... and incubated with chicken IgY anti-DMPO (20μg/mL) and rat IgY anti-mouse CD8a (1:250, SouthernBiotech). DMPO adducts were detected with Alexa Fluor 488–conjugated goat anti-chicken IgY secondary antibody (1:400 ...
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bioRxiv - Microbiology 2022Quote: ... a rabbit anti-rat conjugated to horseradish peroxidase was used as a secondary Ab (1:1000; Southern Biotech) and incubated with TBST (5% BSA ...
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bioRxiv - Immunology 2020Quote: ... all serum samples were diluted to 1 total IgG ELISA unit and then detected with anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam). The results of the IgG subclass ELISA are presented using OD values instead of the ELISA units used for the total IgG ELISA ...
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bioRxiv - Genetics 2021Quote: ... Primary antibodies (1:250 Anti-Fibronectin-1, F3648, Sigma-Aldrich; 1:100 Goat Anti-Type I Collagen, 1310-01, Southern Biotech; 1:100 Goat Anti-Type V Collagen ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed with 1% BSA-PBS and labeled with APC-conjugated goat anti-rat immunoglobulin antibody (Southern Biotech) at 25°C for 20 min ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibodies used in this study were: horseradish peroxidase (HRP)-conjugated goat anti-rat IgG (Southern Biotech 3010-05), HRP-conjugated goat anti-mouse IgG (Southern Biotech 1010-05) ...
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bioRxiv - Cell Biology 2024Quote: Secondary antibodies for immunoblotting were horseradish peroxidase (HRP)-conjugated goat anti-rat immunoglobulin (Ig) (Southern BioTech Cat#3010-05), HRP-conjugated goat anti-rabbit Ig (Southern BioTech Cat#4010-05 ...
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bioRxiv - Biochemistry 2020Quote: ... the culture media were tested for their ACE2-Fc contents using a custom ELISA (anti-ACE2 antibody [Abcam, Cambridge, MA] for capturing and anti-human IgG-Fc-HRP [SouthernBiotech, Birmingham, AL] for detection). Clones with the highest expression of recombinant ACE2-Fc variants were selected ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were washed in phosphate buffered saline containing 0.05% Tween 20 (PBST) and incubated with 10% rabbitserum and then goat anti-type V collagen antibody (Southern Biotech, Birmingham, AL) at 1:1000 for 1.5 hours ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed and the samples were incubated with 50 μl of streptavidin-HRP diluted in blocking solution and standards with 50 μl of 0.175 μg/ml rat anti-mouse IgE-HRP (1130-05, Southern Biotech) diluted in blocking solution for 1 hour at RT ...
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bioRxiv - Immunology 2021Quote: ... IgA (Clone 11-44-2, Southern Biotech), and IgM (Clone RMM-1 ...
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bioRxiv - Microbiology 2019Quote: ... with a goat-derived polyclonal anti-rat antibody conjugated to HRP at a dilution of 1:2000 in blocking buffer (Cat. Nr. 3050-05, Southern Biotech). After further washing ...
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bioRxiv - Immunology 2022Quote: ... Hp-specific IgG1 was detected as previously described47 using plates coated with Hp extract and detected using rat anti-mouse IgG1-HRP antibody (Southern Biotech). NP-OVA specific IgG1 was detected as previously described48 using plates were coated with TNP(5)-BSA and detected using goat anti-mouse IgG1-HRP (Southern Biotech).
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bioRxiv - Cell Biology 2023Quote: ... Labeling of primary antibodies was visualized with anti-mouse PE and anti-rat PE (both Southern Biotech, USA, 1:1000 diluted). Actin in living cells was visualized with SiR actin (Spirochrome ...
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bioRxiv - Immunology 2021Quote: To detect mouse Ig Nunc MaxiSorp ELISA plates were coated for 1h at room temperature with goat anti-mouse Ig (H+L) (4 μg/ml; Southern Biotech, Cat#1010-01; RRID: AB_2794121). Citrobacter rodentium-specific Ig was quantified by coating Nunc MaxiSorp ELISA plates with 108 bacteria/well heat-inactivated at 60°C for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... Paraffin sections of kidneys were used to stain for collagen type IV using a goat polyclonal collagen IV antibody (Southern Biotech, Birmingham, AL, USA) (Thallas-Bonke et al. ...
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bioRxiv - Immunology 2020Quote: ... plates were washed with PBS-T and HRP-conjugated goat anti-mouse IgG or anti-rat IgG (Southern Biotech, Birmingham, AL, USA) added for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... followed by two rounds of fluorescence-activated cell sorting (FACS) of cells stained with FITC-Conjugated rat anti-mouse IgG1 (Southern Biotech 1144-02), and rounds of soft-agar cloning in SeaPlaque agarose ...
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bioRxiv - Microbiology 2021Quote: ... The plates were washed a second time and incubated for 2 h with Streptavidin-alkaline phosphatase from Southern Biotech (2 µg/mL). The final wash was followed by the addition of Nitro-blue Tetrazolium Chloride/5-bromo-4-chloro 3 ‘indolyphosphate p-toludine salt (NBT/BCIP chromagen ...
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bioRxiv - Immunology 2024Quote: ... goat F(ab′)2 anti-human kappa FITC and goat F(ab′)2 anti-human lambda FITC (SouthernBiotech; to detect antibody expression), and PI (Invitrogen ...
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bioRxiv - Immunology 2021Quote: Goat F(ab’)2 anti-mouse Igκ (Southern Biotech) was biotinylated with 20-fold molar excess of NHS-LC-LC-biotin (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... (2) block sections in 5% normal goat serum (SouthernBiotech) and 0.3% Triton-X solution in PBS for 1h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... goat F(ab’)2 anti-mouse IgM (αIgM, SouthernBiotech) or rat anti-mouse CD40 (αCD40 ...
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bioRxiv - Cancer Biology 2020Quote: Primary CLL samples were treated with 20 µg/ml anti-IgM F(ab’)2 or control F(ab’)2 (both Southern Biotech, Cambridge Biosciences, UK) for 5 minutes at 37°C/5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... F(ab’)2 fragments or human IgG1 (control) (Southern Biotech) for 1hr at 37°C in a 96-well plate ...
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bioRxiv - Biochemistry 2020Quote: ... 6-diamidino-2-phenylindole (DAPI; Southern Biotech, Birmingham, AL, USA). Images were made using the Zeiss LSM880 (Oberkochen ...
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bioRxiv - Immunology 2022Quote: ... anti-IgG F(ab’)2 (5 μg/mL; Southern Biotech) or anti-IgM F(ab’)2 (5 μg/mL ...
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bioRxiv - Microbiology 2022Quote: ... α-IgA (mAb 11-44-2, FITC, SouthernBiotech catalog no. 1165-02). Cells were stained with primary antibodies (1:50 dilution ...
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bioRxiv - Immunology 2022Quote: ... or anti-IgM F(ab’)2 (5 μg/mL; Southern Biotech). Cells were immediately fixed with pre-warmed paraformaldehyde (4% final concentration) ...
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bioRxiv - Bioengineering 2019Quote: ... for 2 hr and mounted with Fluoromount-G (SouthernBiotech; 0100-20). Imaging was performed on a Nikon A1Rsi confocal microscope.
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed with 1% BSA in PBS twice before incubating with secondary anti-rat IgG antibody conjugated with APC (1:500; Southern Biotech, AL, USA, Catalog no. 3010-11L). Cells were analyzed in BD FACSVerse™ flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... followed by goat F(ab’)2 anti-mouse IgG1-FITC (Southern Biotech). The percentage of living HUCCT1 cells was estimated by flow cytometry ...
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bioRxiv - Immunology 2020Quote: ... Cells were stimulated with anti-mouse IgM-F(ab)2 (Southern Biotech), ionomycin (4μM ...
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bioRxiv - Microbiology 2022Quote: ... or 2 μg/ml goat anti-hu-IgM-FITC (2020-02, SouthernBiotech) for 30 minutes at 4 °C while shaking ...
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bioRxiv - Immunology 2023Quote: ... 20 µg/mL of F(ab’)2 anti-human IgM (Southern Biotech) was added to the cell medium and incubated for the indicated length of time ...
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bioRxiv - Cell Biology 2023Quote: ... goat F(ab’)2 anti-mouse IgG-PE (Southern Biotech, Birmingham, AL); and anti-phospho-AKT (Ser473) ...
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bioRxiv - Immunology 2024Quote: ... goat F(ab′)2 anti-human IgG PE (SouthernBiotech, dilution 1:100), and PI (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... Slides were mounted with DAPI Fluoromount-G (DAPI, 4,6-diamidino-2-phenylindole) (SouthernBiotech). Stained slides were imaged using Leica Application Suite X software on a Leica Sp8 (Leica) ...
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bioRxiv - Neuroscience 2021Quote: ... The slides were mounted using 4,6-diamidino-2-phenylindole (DAPI) Fluoromount-G (SouthernBiotech) and sealed with a coverslip ...
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bioRxiv - Neuroscience 2022Quote: ... then 2 times with 1X PBS before mounting with Fluoromount-G (Southern Biotech) on slides with a 0.5mm spacer between the slide and coverslip ...
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bioRxiv - Immunology 2023Quote: Biotinylated Goat F(ab)2 anti-human isotype antibodies (anti-human IgM, SouthernBiotech, Cat # 2022-01 ...