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Citations for SouthernBiotech :
651 - 700 of 868 citations for Irisin Human Mouse Rat HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... were pre-coated with 2μg/mL of goat-α-mouse Ig(H+L) (Southern Biotech) diluted in PBS ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed and probed with either anti-mouse IgG (Southern Biotech, Cat. 2040-05) or anti-mouse IgA (Invitrogen Cat ...
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bioRxiv - Molecular Biology 2024Quote: ... The secondary antibody used was goat anti-mouse Ig HRP (Southern Biotech, Cat. #1010-05) diluted 1:2000 in blocking buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Plates were probed using alkaline phosphatase-coupled antibodies against mouse IgM and IgG1 (Southern Biotech). Phosphatase substrate (Sigma ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 5 times with PBS/0.05% Tween20 and IgG antibodies were detected using horseradish peroxidase (HRP)-conjugated goat anti-human IgG detection antibody (Southern Biotech, Birmingham, AL, USA) at a 1:4,000 dilution and incubated for 90 min at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Unbound antibodies were washed away with PBST and bound antibodies detected with 0.5 μg/mL HRP-conjugated goat anti-human constant kappa (2060-05, Southern Biotech, Birmingham, AL, USA) diluted in PBSTB ...
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bioRxiv - Microbiology 2021Quote: ... incubated with primary antibody (starting concentration 10 µg/ml) in PBS for 120 min and secondary antibody (anti-human IgG-HRP; Southern Biotech 2040-05) diluted 1:2500 in 1% BSA in PBS for 60 min at RT ...
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bioRxiv - Microbiology 2021Quote: ... samples were further statically incubated for 1 h at 4°C with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech, 1:300) and 6 μM Syto9 (Live/Dead BacLight Bacterial Viability Kit ...
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bioRxiv - Immunology 2020Quote: ... with excess of PBS-T before being incubated for one hour at RT with an alkaline phosphatase-conjugated goat anti-human IgG (Southern Biotech, Birmingham, AL), diluted 3000 × in PBS-T ...
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bioRxiv - Immunology 2020Quote: ... the plates were first washed 5 times with PBS-T and then incubated for 1 hour at RT with HRP-conjugated anti-human IgG (Fc-specific, clone JDC-10, Southern Biotech, Birmingham, AL) diluted 3000 × in PBS-T ...
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bioRxiv - Immunology 2020Quote: ... the plates were washed at least 5 times with PBS-T and then incubated for 1 hour at RT with HRP-conjugated anti-human IgG (Fc-specific, clone JDC-10, Southern Biotech, Birmingham, AL) diluted 3000 × in PBS-T ...
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bioRxiv - Immunology 2022Quote: ... and the cells were stained with 50 μL/well of a 1:1,000 dilution of goat anti-human IgG-PE (Southern Biotech, cat. # 2040-09) secondary antibodies for 30 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... and stained with a 1:1,000 solution of goat anti-human IgG antibodies conjugated to phycoerythrin (PE) (Southern Biotech, Cat# 2040-09; RRID:AB_2795648) at 4°C for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... cells were washed twice with FACS buffer and incubated for 30 min at 4°C with 1:1000 diluted PE-conjugated goat F(ab)’2 anti-human IgG (Southern Biotech 2042-09) in the dark ...
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bioRxiv - Immunology 2023Quote: ... the plates were incubated with HRP-conjugated goat anti-human IgG and IgA secondary Abs (Southern Biotech, IgG: 2044-05, IgA: 2053-05) and developed by the TMB substrate (ThermoFisher) ...
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bioRxiv - Immunology 2023Quote: ... and incubated with 0.75x serial dilutions of purified polyclonal human antibodies (IgG, SouthernBiotech, Cat # 0150-01; IgM, SouthernBiotech, Cat # 0158L-01; IgA, SouthernBiotech, Cat # 0155K-01) at known concentrations ...
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bioRxiv - Immunology 2023Quote: ... and the development of ADA in plasma samples were detected using a goat anti-human lambda IgG HRP-conjugated secondary antibody at 1:4000 dilution (Southern Biotech, Birmingham, AL). The plasma ADA levels were reported as endpoint titer ...
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bioRxiv - Genetics 2021Quote: ... Wells were washed as described and biotin conjugated goat anti-human IgG (Jackson AffiniPure #109-065-098; 1:30,000 or Southern Biotech 2049-08; 1:1,000) in PBS/1.0% BSA detection antibody was added to the wells at 100ul/well and incubated for 2 hours at room temperature with shaking ...
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bioRxiv - Biochemistry 2020Quote: ... the culture media were tested for their ACE2-Fc contents using a custom ELISA (anti-ACE2 antibody [Abcam, Cambridge, MA] for capturing and anti-human IgG-Fc-HRP [SouthernBiotech, Birmingham, AL] for detection). Clones with the highest expression of recombinant ACE2-Fc variants were selected ...
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PD-1 checkpoint blockade disrupts CD4 T cell regulated adaptive B cell tolerance to foreign antigensbioRxiv - Immunology 2021Quote: ... wells were coated with 50 µl of 25 µg/mL unlabeled goat anti-mouse IgG (SouthernBiotech), anti-mouse IgG1 (RMG1-1 ...
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bioRxiv - Immunology 2021Quote: ... The plates were washed and HRP-conjugated goat anti-mouse IgG (Southern Biotech, Birmingham, AL, USA) was added to the wells for 1 h at 37°C ...
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bioRxiv - Immunology 2022Quote: ... the plate was incubated with goat anti-mouse secondary antibodies for IgG (Southern Biotech; 1030-05), IgG1 (Southern Biotech ...
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bioRxiv - Evolutionary Biology 2021Quote: ... IgA was detected using horseradish peroxidase (HRP)-conjugated goat anti-mouse IgA (Southern Biotech #1040-05), in PBS/1 %BSA/0.01% Tween-20 (100 μL ...
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bioRxiv - Immunology 2021Quote: ... yoelii recombinant MSP-142 and AMA-1 or 10 μg/ml anti-mouse Kappa (Southern Biotech). Cells were incubated for 16-24 h at 37°C ...
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bioRxiv - Immunology 2021Quote: ... were coated at 0.5µg/mL with goat anti-mouse IgG2b (Southern Biotech, catalog no 1090-05) overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... 100 μl of horseradish peroxidase (HRP)-conjugated goat anti-mouse secondary antibody (Southern Biotech #1036-05) was then added at a dilution of 1:5000 and incubated for 1 h at ambient temperature ...
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bioRxiv - Immunology 2022Quote: ... and IgG binding was detected using a PE-conjugated mouse anti-monkey IgG (SouthernBiotech, Birmingham, Alabama) at 2 µg/ml ...
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bioRxiv - Microbiology 2022Quote: ... or mouse anti-hamster-IgG2/3-HRP (Southern Biotech, Cat# 1935–05, 1:8000 in PBST) or mouse anti-hamster-IgG1-HRP (Southern Biotech ...
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bioRxiv - Immunology 2020Quote: ... Plates were incubated with serially diluted HRP-conjugated goat anti-mouse IgG (Southern Biotech #1030-05) in PBS/1%BSA/0.01% Tween-20 (100 µL ...
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bioRxiv - Immunology 2020Quote: ... Immunoglobulins were detected using horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG1 (Southern Biotech #1070-05), IgG2b (Southern Biotech #1090-05) ...
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bioRxiv - Microbiology 2019Quote: ... We used alkaline phosphatase-conjugated goat α-mouse IgG at 1:2000 (Southern Biotech, Birmingham, AL), and visualized using alkaline phosphatase substrate [Tris-base ...
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bioRxiv - Immunology 2019Quote: ... Specific antibodies were detected with alkaline phosphatase-conjugated goat anti-mouse IgG (Southern Biotech [1030-04]). The monoclonal antibodies Y2 ...
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bioRxiv - Immunology 2021Quote: ... or mouse anti-hamster-IgG2/3-HRP (Southern Biotech, Cat# 1935-05, 1:8000 in PBST). Optical density was measured at 490 nm and 630 nm using an ELX800 plate reader (Biotek Instruments) ...
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bioRxiv - Cancer Biology 2021Quote: ... Binding was detected using an HRP-conjugated goat anti-mouse IgG (Southern Biotech, cat # 1031-05) secondary antibody added at a 1 ...
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bioRxiv - Microbiology 2021Quote: ... or mouse anti-hamster-IgG2/3-HRP (Southern Biotech, Cat# 1935-05, 1:8000 in PBST) or mouse anti-hamster-IgG1-HRP (Southern Biotech ...
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bioRxiv - Immunology 2020Quote: ... and 100µl of a goat anti-Mouse IgG horseradish peroxidase (HRP) conjugated secondary antibody (Southern Biotech) diluted 1:5,000 in PBS-T was added to each well ...
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bioRxiv - Immunology 2021Quote: ... IgH μ heavy chains were detected with goat anti-mouse μ conjugated to alkaline phosphatase (SouthernBiotech) and p-nitrophenyl phosphate substrate.
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bioRxiv - Immunology 2022Quote: ... Anti-commensal Ig antibodies were detected with horseradish peroxidase-conjugated antibodies against mouse Ig (Southern Biotech). Plates were developed in the dark with 100 ul of tetramethylenbenzidine per well (KPL) ...
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bioRxiv - Immunology 2022Quote: ... and RBD binding was revealed using secondary goat anti-mouse IgG-AP (Southern Biotech, 1032-04). After washing ...
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bioRxiv - Neuroscience 2022Quote: ... incubated with primary antibody overnight and then anti-mouse IgG1 (Southern Biotech 1071-08, 1:1000) in 20% Superblock (Fisher PI-37535 ...
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bioRxiv - Immunology 2022Quote: ... The secondary antibody for the rabbit plasmais PE-conjugated polyclonal mouse anti-rabbit IgG (Southern Biotech), and that for the CytoGam is mouse anti-human IgG (Southern Biotech) ...
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bioRxiv - Biochemistry 2023Quote: ... followed by the addition of 1/10,000 goat anti-mouse IgA-biotin (Southern Biotech, #1040-08) or 1/10,000 goat anti-IgG-biotin (Southern Biotech #1030-08 ...
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bioRxiv - Immunology 2022Quote: ... beads were incubated for 1 hour with PE-conjugated goat anti-mouse IgG Abs (Southern Biotech). After three washes ...
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bioRxiv - Cancer Biology 2023Quote: ... NP-specific LLPC spots were visualized using AP-conjugated goat anti-mouse IgG1 (SouthernBiotech; 1071-04) with enzyme substrates.
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bioRxiv - Immunology 2023Quote: ... and RBD binding was revealed using secondary goat anti-mouse IgG-AP (Southern Biotech, 1032-04). After washing ...
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bioRxiv - Immunology 2023Quote: ... Bound antibody was detected using Alkaline Phosphatase-conjugated goat anti-mouse IgG (Southern Biotech, Birmingham, AL). The reaction was revealed with a p-Nitrophenyl phosphate disodium salt (Sigma ...
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bioRxiv - Immunology 2024Quote: ... goat anti-mouse IgG-HRP (horseradish peroxidase) (cat. no. 1030-05, Southern Biotech, Birmingham, AL, USA) was added at 1:4,000 in PBST plus BSA ...
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bioRxiv - Immunology 2023Quote: ... plates were washed and alkaline phosphatase (AP)-conjugated goat polyclonal anti-mouse IgG/IgM (Southern Biotech) diluted with 0.2% BSA/PBS (1:1000 ...
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bioRxiv - Microbiology 2021Quote: For the detection of antibody titers in sera and fecal supernatants 96-well plates were coated with goat anti-human Ig (H+L chain) antibody (Southern Biotech, Cat. No. 2010-01) or goat anti-human IgA Fab (Southern Biotech ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed twice with Fc stain buffer and stained with Alexa Fluor 647 goat anti-human IgG (Southern Biotech [Birmingham, AL], cat. 2016-31) in FACS buffer for 30 minutes at 4°C followed by 2 wash steps with FC stain buffer and resuspended in Fluorofix (BioLegend ...