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Citations for SouthernBiotech :
601 - 650 of 868 citations for Irisin Human Mouse Rat HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... followed by goat anti-mouse IgGs coupled to horseradish peroxidase (HRP) (SouthernBiotech, Birmingham, AL). Background optical density (OD450 ...
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bioRxiv - Cell Biology 2022Quote: ... the following antibodies were employed: polyclonal goat anti-mouse lambda (1060-01, Southern Biotech); mouse monoclonal (C8.B6 ...
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bioRxiv - Microbiology 2019Quote: ... Horseradish peroxidase (HRP)-conjugated mouse anti-monkey IgG secondary antibody (Southern BioTech; Birmingham, AL) was used at a 1:4,000 dilution and incubated at 37°C for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... IgG was detected using HRP-conjugated goat anti-mouse IgG2b (Southern Biotech #1090-05) in PBS/1%BSA (100 µL ...
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bioRxiv - Immunology 2020Quote: ... IgG was detected using HRP-conjugated goat anti-mouse IgG (Southern Biotech #1030-05) in PBS/1%BSA/0.01% Tween-20 (100 µL ...
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bioRxiv - Microbiology 2020Quote: ... Horseradish peroxidase (HRP)-conjugated mouse anti-monkey IgG secondary antibody (Southern BioTech; Birmingham, AL) was used at a 1:4,000 dilution and incubated at 37°C for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... and mouse anti-chicken monocyte/macrophage-PE (Clone KUL01, Southern Biotech, Birmingham, AL, USA) antibodies in the dark for 20 min at RT ...
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bioRxiv - Immunology 2020Quote: ... a 1:50,000 dilution of biotinylated mouse anti-monkey IgG was used (Southern Biotech), followed by a 1 h incubation with a 1:3000 dilution of HRP-labeled streptavidin (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... Staining was detected using goat anti-mouse IgG FITC at 1:500 (Southern Biotech) ANAs were scored on intensity of nuclear and cytoplasmic staining on a scale of 0–3 and for the presence or absence of mitotic chromatin using wide-field fluorescence microscopy (Olympus IX83 ...
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bioRxiv - Immunology 2021Quote: ... goat anti-mouse IgM (4 μg/ml; Southern Biotech, Cat#1020-05; RRID: AB_2794201) or goat anti-mouse IgA (4 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight goat anti-mouse IgM (1mg/ml, Southern Biotech, cat# 1021-01) and blocked with coating buffer containing with 1% BSA (w/v ...
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bioRxiv - Immunology 2021Quote: ... Antibodies were detected with HRP-conjugated goat anti-mouse IgG (Southern Biotech, Birmingham, AL) after the enzymatic reaction was developed ...
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bioRxiv - Cell Biology 2022Quote: ... The HRP–conjugated secondary antibodies were purchased from SouthernBiotech (mouse, WB 1:20 000) and Protein A HRP–conjugated were purchased from Invitrogen (WB 1 ...
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bioRxiv - Immunology 2022Quote: ... followed by incubation with goat anti-mouse IgG1- or IgG2a-adsorbed HRP (Southern Biotech) for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... and goat anti-mouse IgG conjugated to HRP (Southern Biotech, 1031-05; 1:10,000). The membrane was again washed three times with TBST and the protein of interest was detected using a chemiluminescence reagent (ECL Prime ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated in Goat-Anti-Mouse HRP-conjugated secondary antibody (SouthernBiotech; 1:2000) for 1 h at room temperature and detected by ECL (PerkinElmer) ...
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bioRxiv - Immunology 2023Quote: ... and ELISAs were detected with HRP-conjugated goat-anti-mouse IgG1-HRP (Southern Biotech). To develop the enzymatic reaction TMB was used as described above ...
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bioRxiv - Immunology 2023Quote: ... plates were coated with capture antibody (goat anti-mouse IgG1, 1 μg/ml, SouthernBiotech) or cetuximab (20 µg/ml ...
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bioRxiv - Microbiology 2022Quote: ... the beads were incubated with 1 µg/mL Alexa Fluor647-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech, 2062-31) in PBS-TH for 30 min at 4 °C with shaking ...
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bioRxiv - Bioengineering 2022Quote: ... We washed with PBS three times to remove the unbound fractions and treated the mixtures with anti-human IgG secondary antibody (SouthernBiotech #2016-05). The plate was further incubated at room temperature for 30 min ...
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bioRxiv - Immunology 2022Quote: ... were coated overnight at 4°C with 2 μg/mL of monkey cross-adsorbed goat anti-human IgG (Southern Biotech #2049-01) diluted in phosphate-buffered saline (PBS) ...
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bioRxiv - Microbiology 2020Quote: ... washed 3 times in ice-cold PBSM and then bound antibody detected with goat anti-human kappa-A647 (SouthernBiotech, cat# 2060-31) After washing ...
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bioRxiv - Cell Biology 2023Quote: ... The presence of the sLAP proteins was verified by western blotting according to standard protocols and by staining with anti-human HRP antibodies (Southern Biotech, USA). Concentration of sLAP proteins was quantified by dot blotting and using purified human Fc-IgG (SigmaAldrich ...
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bioRxiv - Immunology 2023Quote: ... MAB273 was detected by adding a 1:5,000 dilution of monkey cross-adsorbed polyclonal goat anti-human IgG HRP antibody (Southern Biotech, 2049-05) and the signal was developed as described above.
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bioRxiv - Molecular Biology 2020Quote: ... The secondary antibodies used were the following: goat α-mouse-HRP (Southern Biotech, 1:5000) and goat α-rabbit-HRP (Southern Biotech ...
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bioRxiv - Immunology 2021Quote: ... As positive control cells were stimulated with anti-mouse CD3 [0,05 μg/ml] (Southern Biotech) and anti-mouse CD28 antibody [0,5 μg/ml] (Southern Biotech) ...
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bioRxiv - Immunology 2021Quote: ... washed and detected with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (SouthernBiotech, Birmingham, AL). The ELISA plates were developed using SureBlue 3,3′,5,5′-tetramethylbenzidine (TMB ...
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FOXO dictate initiation of B cell development and myeloid restriction in common lymphoid progenitorsbioRxiv - Immunology 2022Quote: ... were coated with 10-20µg/ml of anti-mouse immunoglobulin (Ig) (H+L) (Southern Biotech) to capture total Ig in the serum ...
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bioRxiv - Immunology 2019Quote: ... Plates were then washed and incubated with peroxidase-conjugated goat anti-mouse IgM (Southern Biotech). Spots were revealed with AEC (Sigma-Aldrich) ...
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bioRxiv - Immunology 2019Quote: ... Plates were then washed and incubated with peroxidase-conjugated goat anti-mouse IgM (Southern Biotech). Detection was done with TMB Substrate Reagent Set (BD OptEIA ...
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bioRxiv - Immunology 2021Quote: ... Bound antibodies were detected using peroxidase-conjugated goat anti-mouse IgM heavy chain (Southern Biotech) or IgG (H+L ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed and goat anti-mouse IgG Fc-HRP (Southern Biotech, 1013-05) was added to the plate at 1:2000 in PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... the tissues were washed with PBS and incubated with rabbit-anti-mouse antibody (Southern Biotech) for 30 minutes at room temperature followed by poly-HRP-anti-rabbit IgG (DPVR110HRP ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibody binding was detected with rabbit anti-mouse IgG1-APC (Southern Biotech, Birmingham, USA). To assess binding of recombinant CXCL12γ ...
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bioRxiv - Immunology 2022Quote: ... 100 µL of goat anti-mouse IgG -HRP (1:4,000, Southern Biotech, Birmingham, AL, USA) or goat anti-hamster IgG-HRP (1:32,000 ...
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bioRxiv - Microbiology 2022Quote: ... or mouse anti-hamster-IgG1-HRP (Southern Biotech, Cat# 1940–05, 1:8000 in PBST) for 2 h at RT ...
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bioRxiv - Immunology 2021Quote: ... then incubation with HRP-conjugated goat anti-mouse IgG (catalog number 1030-05, Southern Biotech). Plates were then developed with 3,3’,5,5’-tetramethylbenzidine (TMB ...
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bioRxiv - Microbiology 2021Quote: ... or mouse anti-hamster-IgG1-HRP (Southern Biotech, Cat# 1940-05, 1:8000 in PBST) for 2 h at RT ...
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bioRxiv - Immunology 2019Quote: ... then IgG binding was detected using a PE-conjugated mouse anti-monkey IgG (Southern Biotech) at 4 μg/mL ...
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bioRxiv - Molecular Biology 2019Quote: ... The secondary antibodies used were the following: goat anti-mouse-HRP (1:5000, Southern Biotech) or goat anti-rabbit-HRP (1:5000 ...
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bioRxiv - Microbiology 2020Quote: ... HRP-conjugated goat anti-mouse IgG (Southern Biotech, cat no:1030-05, 1:5000 dilution), HRP-conjuated goat anti-horse IgG (Bellancom life H15034,1:2000 dilution ...
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bioRxiv - Immunology 2021Quote: ... As positive control cells were stimulated with anti-mouse CD3 [0,05 μg/ml] (Southern Biotech). For intracellular staining ...
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bioRxiv - Immunology 2020Quote: ... Detection antibodies were subclass specific goat anti-mouse polyclonal R-PE-conjugated antibodies (Southern Biotech) used at a 1:200 dilution ...
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bioRxiv - Immunology 2021Quote: ... or goat anti-mouse IgA (4 μg/ml; Southern Biotech, Cat#1040-05; RRID: AB_2714213). The reaction was visualized by subsequent addition of 2,2′-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid ...
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bioRxiv - Immunology 2022Quote: ... with 0.5 μg Cy3B-labelled goat IgG1 anti-mouse Igκ (Southern Biotech; labelled in-house), 0.375 μg donkey anti-goat IgG (H+L ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were stained using mouse anti-chicken mABs CD3-PE (clone CT-3; Southern Biotech) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed and incubated at 4°C with goat anti-mouse Ig-PE (SouthernBiotech). Cells were washed and mean fluorescence intensity (MFI ...
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bioRxiv - Immunology 2023Quote: ... Anti-Vi antibody was detected by AP-conjugated goat anti-mouse secondary antibodies (Southern Biotech) at 1:4000 for IgM ...
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bioRxiv - Immunology 2022Quote: ... while biotin-labeled goat anti-mouse IgA Ab (1:5000, Southern Biotech, Cat#1040-08) plus HRP-conjugated streptavidin (1:7000 ...
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bioRxiv - Immunology 2023Quote: ... Captured antibodies were detected using biotinylated goat anti-mouse IgG (Southern Biotech, CAT: 1036-08) and streptavidin-HPR (BioLegend ...