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Citations for SouthernBiotech :
151 - 186 of 186 citations for 5 Fluoro 2 piperidin 2 yl 1H benzimidazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Plates were washed 5 times with PBS-T and incubated with goat anti-mouse IgG1 antibody conjugated with horseradish peroxidase (SouthernBiotech) for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... slides were washed 2 x for 5 minutes in PBS-tx and 3 x for 5 minutes in PBS and cover slipped with DAPI Fluoromount-G (SouthernBiotech).
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed with PBS-T for 5 mins at room temperature and then mounted with DAPI-Fluoromount-G (SouthernBiotech) to adhere coverslips ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed three times in 1X PBS for 5 min before being mounted on slides with Fluoromount-G (SouthernBiotech).
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bioRxiv - Immunology 2024Quote: ... Membranes were incubated in TBST containing 5% (w/v) skimmed milk with goat anti-mouse IgG conjugated to horseradish peroxidase (HRP) (1:2000, 1010-05, SouthernBiotech) for 3h at 4°C in a rotary platform and washed thrice with TBST for 10 min each time ...
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bioRxiv - Immunology 2019Quote: ... Sera were incubated 2 h at 37°C on wells pre-coated with 5 μg/mL of NP15-BSA or goat-anti-mouse IgM (Southern Biotech) and saturated with PBS 2% BSA ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were then rinsed again three to four times in PBS for 5-10 min and mounted on Fisherbrand microscope slides and cover slipped using Fluoromount-G (Southern Biotech) mounting medium.
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bioRxiv - Neuroscience 2020Quote: ... All coverslips were then washed three times (five minutes per wash) in 1xPBS followed by block and permeabilization with 5% (v/v) normal goat serum (NGS, Southern Biotech) and 0.25% (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were subsequently washed three times for 5 min each in PBS and mounted on microscope slides (Brain Research Laboratories) using Fluoromount-G mounting medium (Southern Biotech). Images were obtained with an EVO FL Imaging System equipped with 4x objective to visualize entire ACA with the adjacent cortical areas simultaneously ...
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bioRxiv - Physiology 2020Quote: ... Sections were again washed 3 times 5 minutes with 1x PBS followed by mounting with coverslips using Fluoromount-G mounting media containing DAPI nuclear stain (Southern Biotech).
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bioRxiv - Immunology 2020Quote: ... plates were washed 4 times with PBS-T and an anti-monkey IgG conjugated to horseradish peroxidase (Southern Biotech 4700-5) 1 hour at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... labeled protein was visualized using the AP substrate 5-bromo-4-chloro-indolyl-phosphate/nitro blue tetrazolium (BCIP/NBT; Southern Biotech).
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bioRxiv - Immunology 2022Quote: ... NP-OVA specific IgG1 was detected as previously described48 using plates were coated with TNP(5)-BSA and detected using goat anti-mouse IgG1-HRP (Southern Biotech).
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bioRxiv - Physiology 2023Quote: ... Non-specific antibody binding was blocked for 1 hour at room temperature with a blocking solution containing: 5% normal goat serum (006001, SouthernBiotech™), 0.3% Triton™ X-100 (X100 ...
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bioRxiv - Microbiology 2023Quote: ... Alexa Fluor® Plus 488 secondary antibodies were diluted 1:1,000 in the respective blocking solutions before mounting with 5 µL DAPI Fluoromount-G® (Southern Biotech) and imaging ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Slides were then washed in PBS + 0.1% Triton 3 × 10 min and PBS 1 × 5 min then coverslipped with Fluoromount G (Southern biotech Cat OB10001) under glass cover slips ...
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bioRxiv - Cancer Biology 2020Quote: ... All cells were maintained at 37 ◻C with 5% CO2 and were mycoplasma free as determined a PCR assay (Southern Biotech).
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bioRxiv - Biochemistry 2020Quote: ... for 1 min, washed with PBS again (3X, 5 min) and slides were mounted with Fluoromount-G mounting medium (SouthernBiotech, Birmingham, AL). Images were acquired on a Leica SP5 confocal microscope and analyzed by Fiji ImageJ.
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bioRxiv - Microbiology 2023Quote: ... cells were attached to a poly-L-lysine treated slide before 5 μl of Fluoromount G with DAPI (Cambridge Bioscience, Southern Biotech) was added and coverslip applied ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... at the level of injection (L3-5) as well as slices from corresponding DRGs (20 µm thickness) were directly mounted with DAPI Fluoromount-G® (SouthernBiotech™) and imaged using Axio Scan Z1 ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed with PBS (3 x 5 minutes) and mounted on standard microscope glass slides using DAPI-Fluoromount G (SouthernBiotech, 0100-20). Antibodies and dilutions used were as follows ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were then washed with PBS-BSA and stained with secondary antibodies (5 μM Alexa Fluor® 488 anti-mouse (SouthernBiotech; 1031-30) and 10 μM Alexa Fluor® 647 anti-human (SouthernBiotech ...
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bioRxiv - Neuroscience 2021Quote: ... sections were washed three times with PBS containing 0.3% Tween-20 (PBST) for 10 min and treated with 5% normal donkey serum (NDS; Southern Biotech, cat#0030-01) in PBST for 1 h at room temperature for blocking ...
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bioRxiv - Neuroscience 2022Quote: ... A final wash of 3 × 5 minutes was performed prior to mounting using DAPI Fluoromount-G® (Southern Biotech, Cat No. 0100-20). Slices were imaged on a Zeiss LSM 800 laser confocal scan head mounted on a Zeiss Z1Axio Observer inverted microsope frame (Carl Zeiss ...
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bioRxiv - Biochemistry 2020Quote: ... the plate was washed extensively (5×) with PBS-T and incubated with a HRP-conjugated mouse anti-human IgG (9040-05, clone JDC-10, Southern Biotech, Birmingham, AL) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were then washed a final time with PBST 5 times for 10 min per wash and then mounted with Fluoromount-G (SouthernBiotech, catlog # OB100-01).
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bioRxiv - Neuroscience 2023Quote: ... Cells were then fixed in 4% paraformaldehyde in Hank’s balanced salt solution (HBSS) for 30 min at room temperature and washed 5 times in HBSS before mounting using Fluoromount with DAPI (Southern Biotech, Birmingham, AL).
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bioRxiv - Microbiology 2023Quote: ... Coverslips were then incubated in secondary antibody in blocking buffer for 1 h at RT followed by 3×5 min washes in PBS and then mounted in Fluoromount G with DAPI (Southern Biotech, Birmingham, AL).
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bioRxiv - Immunology 2023Quote: ... primary B cells (107/ml) were stained for 5 min on ice with 10 μg/ml of biotinylated anti-IgM (Southern Biotech, 1021-08) and washed with Imaging Buffer ...
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bioRxiv - Immunology 2023Quote: ... sonicated to disrupt bead:bead aggregates (Bioruptor®: HIGH, 5 cycles, 30” on/off) and coated with different ratios of biotinylated anti-IgM (Southern Biotech, #1021-08) and biotinylated Eα52-68 peptide (Biotin-GSGFAKFASFEAQGALANIAVDKA-COOH ...
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bioRxiv - Neuroscience 2023Quote: ... Tissues were washed three more times for 5 minutes each in PBST and then mounted using DAPI Fluoromount-G (0100-20, Southern Biotech, Birmingham, AL). Slides were imaged using a 20x objective on a Zeiss laser scanning confocal microscope (LSM710 ...
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bioRxiv - Neuroscience 2024Quote: ... The specimens were subjected to a triple wash with PBS and treated with a 5% normal donkey serum (NDS; Southern Biotech, #0030-01) solution in PBST (PBS with 0.1% Triton X ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then fixed in 3.7% paraformaldehyde in Hank’s balanced salt solution (HBSS) for 30 min at room temperature and washed 5 times in HBSS before mounting using Fluoromount with DAPI (Southern Biotech, Birmingham, AL). Images were acquired using a Zeiss LSM 880 inverted confocal microscope (Carl Zeiss ...
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bioRxiv - Immunology 2019Quote: ... 96-well Immulon 4 HBX plates were coated overnight at 4°C with 5 mg/ml polyclonal goat-anti mouse κ (Southern Biotech; 1050-01). Nonspecific binding was blocked with 1% bovine serum albumin in PBS and samples were incubated at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... They were washed six times in washing buffer (PBS with 0.1% Triton X-100) for 20 min and blocked for 1 h in washing buffer containing 5% heat-inactivated goat serum (SouthernBiotech, Cat. No. 0060-01), followed by incubation with the primary antibodies (rabbit anti–GFP ...
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bioRxiv - Immunology 2023Quote: ... Membranes were washed with TBST (1x, 5 min., RT) and incubated with goat anti-rabbit IgG- HRP (Southern Biotech #1021-05, 10 mL, 1:5,000, 1 h, RT). Membranes were washed with TBST (3x ...