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Citations for SouthernBiotech :
101 - 150 of 186 citations for 5 Fluoro 2 piperidin 2 yl 1H benzimidazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The immunoreactivity was detected by incubation with Rabbit F(ab’)2 Anti-Goat IgG(H+L)-HRP (6020-05, Southern Biotech) diluted 3000 × in PBS-T.
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bioRxiv - Immunology 2024Quote: ... membranes were incubated with primary antibodies overnight at 4 °C and during 2 hours at room temperature with horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit IgG (SouthernBiotech, USA). Membranes were then revealed using G:BOX Chemi XL1.4 Fluorescent & Chemiluminescent Imaging System (Syngene ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed again and the nuclei were stained with DAPI (4’,6-diamidino-2-phenylindole) before mounting with Fluoromount-G (Southern Biotech). Primary antibodies against histone H3 methylation on lysine 4 ...
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bioRxiv - Cancer Biology 2024Quote: ... permeabilized with 0.1% Triton X-100 (Fisher Biotech, Hampton, NH, USA), and blocked with PBS supplemented with goat serum (2%, v/v) (Southern biotech) and 0.1% Tween-20 (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: To detect mouse Ig Nunc MaxiSorp ELISA plates were coated for 1h at room temperature with goat anti-mouse Ig (H+L) (4 μg/ml; Southern Biotech, Cat#1010-01; RRID: AB_2794121). Citrobacter rodentium-specific Ig was quantified by coating Nunc MaxiSorp ELISA plates with 108 bacteria/well heat-inactivated at 60°C for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... the wells were incubated for 2 h at 37°C with HRP-mouse anti-human IgG antibody (dilution 1:2,000) (Southern Biotech, Birmingham, AL) followed by the peroxidase substrate ...
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bioRxiv - Microbiology 2022Quote: ... the beads were incubated with 1 µg/mL Alexa Fluor647-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech, 2062-31) in PBS-TH for 30 min at 4 °C with shaking ...
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bioRxiv - Immunology 2022Quote: ... were coated overnight at 4°C with 2 μg/mL of monkey cross-adsorbed goat anti-human IgG (Southern Biotech #2049-01) diluted in phosphate-buffered saline (PBS) ...
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bioRxiv - Biochemistry 2022Quote: ... and then treated with alkaline phosphatase (AP)-conjugated goat anti-rabbit-IgG (Southern Biotech; 1:8000 in TBST with 2% (w/v) bovine serum albumin) ...
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bioRxiv - Physiology 2020Quote: ... The sections were processed for immunostaining of Pan-CK (--) as described above with 4’,6-diamidino-2-phenylindole (DAPI) containing Fluormount-G (SouthernBiotech, Birmingham, AL) to visualize nuclei.
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bioRxiv - Microbiology 2021Quote: ... samples were further statically incubated for 1 h at 4°C with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech, 1:300) and 6 μM Syto9 (Live/Dead BacLight Bacterial Viability Kit ...
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bioRxiv - Microbiology 2021Quote: ... secondary antibody at RT for 2h and stained with 4’,6-diamidino-2-phenylindole (DAPI) before mounting onto slides with Fluoromount-G (Southern Biotech, Birmingham, AL).
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bioRxiv - Molecular Biology 2020Quote: ... Worms were rinsed in PBS (3×10mins, 3 × 1hr) and equilibriated in mounting media with 4’,6-diamidino-2-phenylindole DAPI (Fluoromount G, Southern Biotech, Birmingham, AL) overnight before mounting and imaging.
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bioRxiv - Developmental Biology 2019Quote: ... A 1 μg/μl 4’6-diamidino-2-phenylindole (DAPI) solution was applied to the slides for nuclear staining prior to mounting in Fluoromount-G (Southern Biotech, 0100-01) with 34×60 mm coverslips (VWR ...
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bioRxiv - Immunology 2022Quote: ... cells were washed twice with FACS buffer and incubated for 30 min at 4°C with 1:1000 diluted PE-conjugated goat F(ab)’2 anti-human IgG (Southern Biotech 2042-09) in the dark ...
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bioRxiv - Immunology 2022Quote: ... ELISA plates were coated with 1 μg/ml recombinant SARS-CoV-2 S protein (43) and serially diluted serum samples were added and detected via anti-monkey IgG-HRP (Southern Biotech, Birmingham, AL). Plates were developed using a TMB substrate (source ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Plates were incubated with serially diluted serum or gut content (50 μL, PBS 1% BSA, wt/vol, 2 h, RT) and standard mouse IgA (Southern Biotech #0106-01, prepared in duplicates) and washed (5x ...
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bioRxiv - Immunology 2020Quote: ... Plates were incubated with serially diluted mouse sera (50 µL, PBS 1% BSA, wt/vol, 2 h, RT) and standard mouse IgG (Southern Biotech #0107-10, prepared in duplicates) and washed (5x ...
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bioRxiv - Bioengineering 2022Quote: ... 5 µL of blocking antibody (anti-IgG, Southern Biotech) was added to the 50 µL cell solution to yield a final concentration of 25 µg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... 50 µm mouse brain sections were rinsed in PBS for 5 minutes and blocked with PBS/5% normal goat serum (Southern Biotech, 0060-01) for 2 hours at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... we washed the samples 5 times for 5 min with PBS and added one drop of fluoromount-G mounting medium (SouthernBiotech, Cat. No.: 0100-01) to each well prior to applying a cover slip.
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bioRxiv - Cell Biology 2023Quote: ... 5 min each and mounted in Fluoromount-G (Southern Biotech). Confocal analysis was performed on a Zeiss LSM 780 inverted microscope ...
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bioRxiv - Systems Biology 2023Quote: ... followed by 5 min Hoechst incubation and mounting with Fluoromount (Southern Biotech). Images were taken using an Olympus BX63 microscope and analyzed in ImageJ.
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bioRxiv - Immunology 2023Quote: ... or 5 µg/mL of goat anti-mouse IgG/IgM (Southern Biotech) overnight at 4℃ ...
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bioRxiv - Cancer Biology 2021Quote: ... before counterstaining with DAPI (5 min) and mounting using Fluoromount G (SouthernBiotech, Cambridge, UK). Slides were imaged with an Axioscan.Z1 (Zeiss ...
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bioRxiv - Microbiology 2020Quote: ... followed by goat anti-human kappa-Alexa Fluor 647 (5 μg/ml, Southern biotech) for another 20 minutes at 4°C to allow discrimination between cell adherent (FITC+/Alexa fluor 647+ ...
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bioRxiv - Cell Biology 2021Quote: ... wells were washed then incubated for 5 mins in DAPI Fluoromount G (Southern Biotech) to stain nuclear and kinetoplastid DNA ...
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bioRxiv - Biochemistry 2023Quote: ... in PBS for 5 min and mounted the cells with Fluoromount-G (Southern Biotech). For negative control ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed again 3x 5 minutes in 1xPBS and mounted with Fluoromount-G (SouthernBiotech). Tissue or cells lacking the SOG-Y transgene were used as a negative control ...
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bioRxiv - Neuroscience 2021Quote: ... further washed 3×5 min in PBS and mounted in Fluoromount-G (SouthernBiotech, 0100-01). Images were acquired with a Zeiss LSM700 confocal laser scanning microscope and assembly was performed with ImageJ/Fiji ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed and incubated for 5 min in ice with streptavidin (60 μg/ml, SouthernBiotech) for cross-linking and incubated at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were rinsed three times (5 minutes each) and coverslipped using mounting medium containing DAPI (SouthernBiotech). Images were taken using a Leica TCS SP8 confocal microscope.
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bioRxiv - Cell Biology 2020Quote: ... for 5 min for nuclei staining and coverslipped using Fluoromount G (Southern Biotech, Birmingham, AL, USA). For hyaluronic acid staining ...
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bioRxiv - Immunology 2021Quote: ... Wells were washed 5 times and secondary goat anti-IgM-HRP (Southern Biotech, cat# 1021-08) at 1:5000 dilution was incubated for 1 hr in the wells ...
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bioRxiv - Neuroscience 2023Quote: ... The slides were washed 3 × 5 mins and mounted with Fluoromount-G (SouthernBiotech, Cat # 0100-01) on microscope slides with Secure-Seal spacer (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Slides were washed 1X PBS before mounting with 5 µL DAPI Fluoromount-G® (Southern Biotech) and imaging ...
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bioRxiv - Microbiology 2023Quote: ... followed by detection with goat-anti human kappa-Alexa Fluor 647 (5 µg/ml, Southern Biotech) and analysis by flow cytometry (BD FACSVerse).
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... slides were counterstained with DAPI for 5 min and enclosed in Fluoromount-G (0100-01; Southern Biotech).
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bioRxiv - Microbiology 2021Quote: ... Plates were then washed 5 times and incubated with goat anti-mouse Ig conjugated to HRP (SouthernBiotech) for 1 hour at room temp ...
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bioRxiv - Cell Biology 2022Quote: ... incubated in Hoechst for 5 min at RT and mounted with coverslips using Fluoromount-G (Southern Biotech). For wax preservation ...
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bioRxiv - Immunology 2022Quote: ... Nunc Immuno Plate MaxiSorp plates were coated with 5 μg/ml anti–Ig capture antibody (Southern biotech) and incubated overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... 500 μL of a secondary antibody solution (5 μM Alexa Fluor® 488 anti-mouse (SouthernBiotech; 1031-30) and 10 μM Alexa Fluor® 647 anti-human (SouthernBiotech ...
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bioRxiv - Cell Biology 2021Quote: ... FB lobes of approximately 5 larvae were mounted in 20 µl DAPI fluoromount-G (Southern Biotech 0100-20) per slide and imaged on an ApoTome.2 system using an Axiovert 200 equipped with a 63x 1.4 NA oil lens (Carl-Zeiss ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were washed four times for 5 min in PBS and embedded in DAPI Fluoromount-G (Southern Biotech).
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bioRxiv - Molecular Biology 2020Quote: ... the following secondary antibodies were used diluted 1:1000 in 5%-milk: anti-mouse (Southern Biotech, 1031–05) and a 1:1 mix of anti-rabbit (Southern Biotech ...
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bioRxiv - Biochemistry 2023Quote: ... or a 5-fold dilution series starting at 200ng/ml of purified IgG (Catalog #0107-01, Southern Biotech) or IgA (Catalog #0106-01 ...
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bioRxiv - Immunology 2020Quote: ... coated with 100 µL/well of goat anti-mouse IgA (5 µg/mL Bic 0.1M, pH 9.6; Southern Biotech) were incubated with serial 3-fold dilutions of either fecal supernatant (100 µL ...
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bioRxiv - Developmental Biology 2021Quote: ... The sections were then washed three times for 5 min with PBST and slides were mounted with Fluoromount-G containing DAPI and photobleaching inhibitors (SouthernBiotech). All steps were performed in a dark humid chamber to prevent evaporation and photobleaching ...
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bioRxiv - Cell Biology 2021Quote: ... coverslips were washed in PEM supplemented with 0.01% Triton-X-100 for 5 minutes before mounting on clean slides using DAPI Fluoromount G (Southern Biotech) or Prolong Gold.
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bioRxiv - Immunology 2021Quote: ... at 1×106 cells/well and stimulated for 48 hours with 5 μg/mL of RBD-His or hCD81-His or media alone (RPMI + 5% FCS) in the presence of anti-mouse CD28 antibody [0,5 μg/ml] (Southern Biotech). As positive control cells were stimulated with anti-mouse CD3 [0,05 μg/ml] (Southern Biotech) ...