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Citations for SouthernBiotech :
951 - 1000 of 1233 citations for Goat Anti Mouse IgG Fc Alkaline Phosphatase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... The sections were washed in phosphate buffered saline containing 0.05% Tween 20 (PBST) and incubated with 10% rabbitserum and then goat anti-type V collagen antibody (Southern Biotech, Birmingham, AL) at 1:1000 for 1.5 hours ...
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bioRxiv - Microbiology 2020Quote: ... washed 3 times in ice-cold PBSM and then bound antibody detected with goat anti-human kappa-A647 (SouthernBiotech, cat# 2060-31) After washing ...
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bioRxiv - Immunology 2020Quote: ... or F(ab)2 polyclonal goat anti-human IgM (Jackson Immuno Research) as capture antibody and detection with HRP-conjugated mouse μ-specific anti-human IgM (Southern Biotech, clone SA-DA4).
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bioRxiv - Microbiology 2022Quote: ... Normal rabbit IgG (SouthernBiotech, Cat# 0111-01, 1:100) was used as negative controls ...
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bioRxiv - Immunology 2021Quote: ... HRP- conjugated IgG or IgM (Southern Biotech, Birmingham, AL) was incubated on the plate for 1 h at 37°C ...
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bioRxiv - Immunology 2020Quote: ... IgG and/or IgM (Southern Biotech; Birmingham, AL, USA), was added at 3 μg/mL ...
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bioRxiv - Microbiology 2020Quote: ... IgG and/or IgM (Southern Biotech; Birmingham, AL, USA). After 30 minutes of incubation at 800 rpm in the dark ...
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bioRxiv - Microbiology 2022Quote: ... Normal rabbit IgG (SouthernBiotech, Cat# 0111-01, 1:100) was used as negative controls ...
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bioRxiv - Immunology 2020Quote: ... Biotinylated rabbit control IgG (Southern Biotech, Birmingham, AL, USA) was used as a negative control ...
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bioRxiv - Synthetic Biology 2023Quote: ... an IgG isotype control (Southern Biotech Catalog# 0109-30) to correct the MFI of AF488 for non-specific staining.
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bioRxiv - Microbiology 2023Quote: ... Normal rabbit IgG (SouthernBiotech, Cat# 0111-01, 1:100) was used as negative control ...
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bioRxiv - Biophysics 2020Quote: The anti-mouse-Igκ antibody for clustering BCRs was purchased from Southern Biotech (Birmingham, AL; catalogue #1050-01). AF647-conjugated anti-mouse-IgM Fab fragments (catalogue #115-607-020 ...
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bioRxiv - Biochemistry 2021Quote: ... 500 μL of a secondary antibody solution (5 μM Alexa Fluor® 488 anti-mouse (SouthernBiotech; 1031-30) and 10 μM Alexa Fluor® 647 anti-human (SouthernBiotech ...
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bioRxiv - Molecular Biology 2020Quote: ... the following secondary antibodies were used diluted 1:1000 in 5%-milk: anti-mouse (Southern Biotech, 1031–05) and a 1:1 mix of anti-rabbit (Southern Biotech ...
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bioRxiv - Immunology 2022Quote: ... Plates were then washed and incubated with 50μL of anti-mouse IgA-HRP (SouthernBiotech, 1:1000 in PBT), washed and developed using 50 μL of TMB substrate (BioLegend) ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed and incubated with 50μL of anti-mouse IgA-HRP (1:1000) in assay buffer (SouthernBiotech). Plates were washed and developed using TMB substrate (BioLegend) ...
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bioRxiv - Biochemistry 2023Quote: ... incubated with an appropriate peroxidase-labeled secondary antibody (anti-mouse IgG1-HRP #1070-05, Southern Biotech, Birmingham, USA) for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... Then samples were washed three times with 0.1% Triton X-100 in PBS for 5 minutes and were incubated 30 minutes at RT with the following secondary antibody diluted 1:200: goat anti-rabbit Alexa Fluor 488 (Southern Biotech 4050-30), goat anti-mouse Alexa Fluor 488 (Southern Biotech 1030-30 ...
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bioRxiv - Immunology 2021Quote: ... Unbound antibodies were washed away with PBST and bound antibodies detected with 0.5 μg/mL HRP-conjugated goat anti-human constant kappa (2060-05, Southern Biotech, Birmingham, AL, USA) diluted in PBSTB ...
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bioRxiv - Microbiology 2021Quote: ... samples were further statically incubated for 1 h at 4°C with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech, 1:300) and 6 μM Syto9 (Live/Dead BacLight Bacterial Viability Kit ...
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bioRxiv - Bioengineering 2021Quote: ... The slides were subsequently incubated at room temperature with secondary antibodies: goat anti-rabbit Ig (1:100 dilution for 15 minutes, 4010-05, Southern Biotech, Birmingham, AL) for CD4 and goat anti-rabbit (1:50 dilution for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... standards (IgM and IgG from Southern Biotech; IgE from Biolegend) and samples were applied to the coated plate for 1 hr at room temperature (RT) ...
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bioRxiv - Microbiology 2022Quote: ... Normal rabbit IgG (Southern Biotech, Cat# 0111-01, 1:100) was used as a negative control ...
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bioRxiv - Microbiology 2022Quote: ... Normal rabbit IgG (Southern Biotech, Cat# 0111-01, 1:100) was used as a negative control ...
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bioRxiv - Microbiology 2023Quote: ... Normal rabbit IgG (Southern Biotech, Cat# 0111-01, 1:100) was used as a negative control ...
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bioRxiv - Microbiology 2023Quote: ... Normal rabbit IgG (Southern Biotech, Cat# 0111-01, 1:100) was used as a negative control ...
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bioRxiv - Microbiology 2021Quote: ... samples were diluted at 1:50 and antigen-coupled microspheres were incubated with secondary antibody diluted at 1:100 each: mouse anti-human IgG1 hinge (0.1 mg/ mL, clone 4E3, SouthernBiotech); IgG2 Fc (0.1 mg/ mL ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then surface stained with mouse anti-chicken Bu-1-FITC (Clone AV20; Southern Biotech, Birmingham, AL, USA) and mouse anti-chicken monocyte/macrophage-PE (Clone KUL01 ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were stained again for 30 min with 0.5 µg of FITC-conjugated anti-mouse IgA antibody (Southern Biotech) in 100 µl of staining buffer to refresh and harmonize the staining between batches ...
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bioRxiv - Molecular Biology 2020Quote: ... and goat α-rabbit-HRP (Southern Biotech, 1:5000).
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bioRxiv - Immunology 2019Quote: ... A secondary polyclonal goat antibody (Southern Biotech, Birmingham, AL) directed to the human kappa light chain and conjugated with horseradish peroxidase (HRP ...
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bioRxiv - Microbiology 2020Quote: ... 1:250 ColIV (polyclonal goat, 1340-01, Southern Biotech), 1:250 CD31 (monoclonal mouse ...
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bioRxiv - Immunology 2023Quote: ... After blocking with 10% goat serum (SouthernBiotech; Birmingham, AL), samples were incubated with mouse anti-PI(3,4)P2 mAB or mouse anti-PI(3,4,5)P3 mAB conjugated with FITC for 1 hour at 37°C [24] ...
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bioRxiv - Bioengineering 2023Quote: ... (2) block sections in 5% normal goat serum (SouthernBiotech) and 0.3% Triton-X solution in PBS for 1h at room temperature ...
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bioRxiv - Microbiology 2021Quote: For the detection of antibody titers in sera and fecal supernatants 96-well plates were coated with goat anti-human Ig (H+L chain) antibody (Southern Biotech, Cat. No. 2010-01) or goat anti-human IgA Fab (Southern Biotech ...
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bioRxiv - Bioengineering 2021Quote: ... and then for 1 hour at room temperature with a 1:4000 diluted rabbit anti-mouse HRP secondary antibody (SouthernBiotech Cat# 6170-05 ...
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bioRxiv - Immunology 2022Quote: ... A volume of 25 μL of a 1:1,000 dilution of Mouse Anti-Biotin-AP (Southern Biotech, cat. #6404-04) in blocking buffer was added to each of the wells of the HN plates and incubated for 1 hr at RT ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed and the samples were incubated with 50 μl of streptavidin-HRP diluted in blocking solution and standards with 50 μl of 0.175 μg/ml rat anti-mouse IgE-HRP (1130-05, Southern Biotech) diluted in blocking solution for 1 hour at RT ...
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bioRxiv - Microbiology 2023Quote: ... was used except that plates were coated with anti-human kappa and lambda light chain specific mouse antibodies (Southern Biotech) at 1:1 ratio diluted to 1 μg/ml in PBS overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 × 105 cells in 100 μL per well were plated in a 96-well plate pre-coated with anti-mouse CD3ε (1 μg/mL, SouthernBiotech). Tumors were harvested from mice 10 days post inoculation and dissociated using gentleMACS (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2023Quote: ... bound S8V1-157 was detected by incubating the microspheres with 2 μg/ml PE-conjugated rat anti-mouse IgG1 (SB77e, SouthernBiotech) for 1 hr at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... PVDF membranes containing blotted proteins were washed in excess PBS-Tween 20 for 1h at room temperature before application of a 1:2000 dilution of primary mouse Anti-Human IgA1 antibodies conjugated to biotin (SouthernBiotech). Blots were washed 10 min 3 x in PBS followed by treatment with a 1:2000 dilution of ExtrAvidin®−Peroxidase conjugate (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed with 1% BSA in PBS twice before incubating with secondary anti-rat IgG antibody conjugated with APC (1:500; Southern Biotech, AL, USA, Catalog no. 3010-11L). Cells were analyzed in BD FACSVerse™ flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: Antigen-specific IgG (Cat. No. 1015-05, Southern Biotech, AL, USA), IgA (Cat ...
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bioRxiv - Bioengineering 2021Quote: ... Wells were incubated with 100μL IgG-biotin HU adsorbed (Southern Biotech) for 2hr at RT ...
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bioRxiv - Immunology 2022Quote: ... the hACE2 receptor bound to the S protein was then detected using a mouse anti-His-Tag horseradish peroxidase conjugate (Southern Biotech) and a colorimetric signal generated by the addition of 3,3′,5,5′-TMB substrate ...
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bioRxiv - Immunology 2022Quote: ... Hp-specific IgG1 was detected as previously described47 using plates coated with Hp extract and detected using rat anti-mouse IgG1-HRP antibody (Southern Biotech). NP-OVA specific IgG1 was detected as previously described48 using plates were coated with TNP(5)-BSA and detected using goat anti-mouse IgG1-HRP (Southern Biotech).
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bioRxiv - Immunology 2022Quote: ... NP-specific IgM and IgG3 antibodies were detected using biotinylated anti-mouse IgM- or IgG3-specific immunoglobulins followed by streptavidin-HRP (Southern Biotech) and developed with SIGMAFAST OPD tablets (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... 1×109 bacteria were washed with 1 ml ice-cold PBS and stained for 30 min at 4°C with 4 µg of FITC-conjugated anti-mouse IgA antibody (Southern Biotech) in 100 µl of staining buffer ...
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bioRxiv - Immunology 2024Quote: ... The secondary antibodies used for western blot analysis were goat anti-rabbit conjugated to horseradish peroxidase-conjugated (HRP) (#4050-05, diluted at 1:1000) and anti-mouse-HRP (#1031-05, diluted at 1:1000) from SouthernBiotech (USA).