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Citations for SouthernBiotech :
851 - 900 of 1233 citations for Goat Anti Mouse IgG Fc Alkaline Phosphatase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... with HRP-conjugated anti-mouse secondary at 1:5,000 dilution (Southern Biotech). The protein antibody complex was visualized using SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... IgG or IgA (Southern Biotech) diluted in PBS 1% BSA ...
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bioRxiv - Immunology 2021Quote: ... IgM and IgG (Southern Biotech). Plates were incubated with corresponding anti-mouse HRP antibodies and TMB substrate was neutralized after 10 min with 0.2 N HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... or rabbit IgG (Southern Biotech) overnight ...
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bioRxiv - Immunology 2022Quote: ... IgG or IgA (Southern Biotech), while TNP-BSA (load 5 ...
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bioRxiv - Immunology 2021Quote: ... and incubated with chicken IgY anti-DMPO (20μg/mL) and rat IgY anti-mouse CD8a (1:250, SouthernBiotech). DMPO adducts were detected with Alexa Fluor 488–conjugated goat anti-chicken IgY secondary antibody (1:400 ...
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bioRxiv - Microbiology 2022Quote: ... The membranes were washed and incubated with the secondary HRP-conjugated anti-rabbit (1:10,000) or anti-mouse (1:5,000; SouthernBiotech) antibodies for 1 h at room temperature ...
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bioRxiv - Immunology 2021Quote: ... 25 μl of 1:5000 diluted horse radish peroxidase (HRP) conjugated anti-Human IgG secondary antibody (Southern Biotech) was added and incubated for 1h at RT ...
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bioRxiv - Immunology 2021Quote: ... The secondary antibody used was horseradish-peroxidase (HRP)-conjugated goat polyclonal antibody against mouse IgG1 (1070-05; Southern Biotech, Cambridge, United Kingdom), HRP-conjugated goat polyclonal antibody against rabbit IgG (4050-05 ...
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bioRxiv - Microbiology 2022Quote: ... and incubated with 0.5 µg/mL Alexa Fluor647-conjugated goat F(ab’)₂ anti-human kappa (Southern Biotech) in RPMI-H for 30 min at 4 °C under shaking conditions ...
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bioRxiv - Microbiology 2022Quote: ... Bound antibodies were detected with horseradish peroxidase (HRP)-conjugated goat F(ab’)₂ anti-human kappa (Southern Biotech) in 1% BSA in PBS-T and Tetramethylbenzidine as substrate ...
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bioRxiv - Immunology 2021Quote: ... after which the intensity was enhanced using a tertiary rabbit anti-goat HRP-conjugated antibody (Southern Biotech). AEC (3-Amino-9-EthylCarbazole ...
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bioRxiv - Plant Biology 2024Quote: ... Washed membranes were incubated in a 1:6000 dilution of goat anti-rabbit secondary antibody (Southern Biotech) conjugated to alkaline phosphatase in 3% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... followed by phycoerythrin (PE)-conjugated anti-mouse antibody (1:200) (Cat#1031309, SouthernBiotech) or anti-caspase-3-phycoerythrin antibody (Clone#C92-605 ...
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bioRxiv - Microbiology 2021Quote: ... in PBST or 1:1000 of anti-mouse IgA-HRP in PBSTBA (SouthernBiotech). Plates were incubated at room temperature for 1 h ...
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bioRxiv - Immunology 2022Quote: ... cells secreting anti-NP antibody (ASC) were visualized with HRP-conjugated anti-mouse IgM or IgG3 antibodies (Southern Biotech) followed by AEC staining Kit (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed three times and then subsequently incubated for 1 h with 0.1 µg ml− 1 of anti-hamster IgG HRP (SouthernBiotech) in casein block at room temperature in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... 50 µm mouse brain sections were rinsed in PBS for 5 minutes and blocked with PBS/5% normal goat serum (Southern Biotech, 0060-01) for 2 hours at room temperature ...
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bioRxiv - Immunology 2021Quote: ... and the total IgG and IgA were determined using the Rhesus Monkey IgG-UNLB (Southern Biotech), and the Monkey IgA ELISA development kit (HRP ...
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bioRxiv - Microbiology 2021Quote: ... IgG and/or IgM (Southern Biotech) was applied ...
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bioRxiv - Immunology 2020Quote: ... or human IgG-HRP (Southern Biotech) at 37°C for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... Bound antibodies were detected with horseradish peroxidase (HRP)-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech) in 1% BSA in PBS-T and Tetramethylbenzidine (TMB ...
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bioRxiv - Bioengineering 2021Quote: ... for CD4 and goat anti-rabbit (1:50 dilution for 30 minutes, 4010-05, Southern Biotech, Birmingham, AL) for CD8 ...
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bioRxiv - Immunology 2019Quote: ... Captured antibodies were detected with secondary R-phycoerythrin-labeled goat-anti-human IgA F(ab’)2 (Southern Biotech) or IgG F(ab’)2 (Jackson Immunoresearch Laboratories) ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed three times and incubated with HRP-conjugated goat anti-human secondary antibody (ITK Southern Biotech) diluted 1:2000 in blocking buffer for 1 hour at room temperature ...
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bioRxiv - Genomics 2021Quote: ... probed again by a goat anti-rabbit secondary antibody (Southern Biotech, catalog # 4030-05, and 1:200 dilution), and developed using a DAB kit (Abcam ...
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bioRxiv - Immunology 2024Quote: ... Human B cell stimulation used 10ng/mL Goat F(ab’)2 Anti-human IgM (Southern Biotech #2022-01), 2ug/mL of recombinant human sCD40 (invitrogen PHP0025) ...
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bioRxiv - Immunology 2020Quote: ... The membrane was washed in PBS containing Tween-20 (0.05%) and was incubated for 1 h with horseradish peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (Southern Biotech) diluted 1:20,000 accordingly ...
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FOXO dictate initiation of B cell development and myeloid restriction in common lymphoid progenitorsbioRxiv - Immunology 2022Quote: ... Serum Ig was detected using a secondary HRP-conjugated anti-mouse IgM (Southern Biotech) or IgG (Southern Biotech ...
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bioRxiv - Microbiology 2021Quote: ... and mouse anti-chicken monocyte/macrophage-PE (Clone KUL01, Southern Biotech, Birmingham, AL, USA) antibodies in the dark for 20 min at RT ...
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bioRxiv - Immunology 2021Quote: ... plates were washed and probed at room temperature for 1 hour with 1μg/ml of biotinylated anti-IgG (Southern Biotech) in PBS 2.5%FCS ...
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bioRxiv - Microbiology 2020Quote: ... followed by addition of 50 μL of 1:1000 anti-hamster-IgG(H+L)-HRP (Southern Biotech Cat. #6061-05) in PBST or PBSTF or 1:1000 anti-hamster-IgG2/IgG3-HRP in PBST or PBSTF (Southern Biotech Cat ...
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bioRxiv - Immunology 2020Quote: An ELISA to detect anti-CCHFV Ig was performed as previously described (Hawman et al., 2019) with an anti-mouse Ig detection antibody (Southern Biotech) to detect all immunoglobulin isotypes or anti-mouse IgG or IgM (Southern Biotech ...
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bioRxiv - Cell Biology 2023Quote: ... Labeling of primary antibodies was visualized with anti-mouse PE and anti-rat PE (both Southern Biotech, USA, 1:1000 diluted). Actin in living cells was visualized with SiR actin (Spirochrome ...
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bioRxiv - Microbiology 2021Quote: ... but the secondary antibody used was an anti-human IgM Peroxidase-labelled antibody produced in goats (SouthernBiotech, Birmingham, USA).
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bioRxiv - Microbiology 2022Quote: ... then incubated in 0.1% TBST containing 1:4000 goat anti-rabbit antibody conjugated to HRP for 1 h at RT (SouthernBiotech).
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bioRxiv - Microbiology 2020Quote: ... followed by staining with goat F(ab’)2 anti-human kappa-Alexa Fluor 647 (5 μg/ml, Southern Biotech) (Supporting figure 1A).
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bioRxiv - Immunology 2020Quote: ... Cells were washed with 1% BSA-PBS and labeled with APC-conjugated goat anti-rat immunoglobulin antibody (Southern Biotech) at 25°C for 20 min ...
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bioRxiv - Microbiology 2023Quote: ... followed by staining with goat F(ab’)2 anti-human kappa-Alexa Fluor 647 (5 μg/mL, Southern Biotech), fixation in PBS 1% formaldehyde and analysis by flow cytometry (BD FACSVerse).
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bioRxiv - Immunology 2023Quote: ... antibody response to individual peptides was detected with different fluorescently labeled secondary antibodies (Goat anti-human IgM-AF488, SouthernBiotech, Cat # 2020-30 ...
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bioRxiv - Microbiology 2024Quote: ... Antibodies used for immunoblot analysis in this study were: anti-Human IgM goat polyclonal antibody (Southern Biotech #2020-01), anti-Human IgG goat polyclonal antibody (Southern Biotech #2040-01) ...
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bioRxiv - Cell Biology 2024Quote: Secondary antibodies for immunoblotting were horseradish peroxidase (HRP)-conjugated goat anti-rat immunoglobulin (Ig) (Southern BioTech Cat#3010-05), HRP-conjugated goat anti-rabbit Ig (Southern BioTech Cat#4010-05 ...
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bioRxiv - Immunology 2021Quote: ... or IgG-AP (SouthernBiotech cat # 1030-04) conjugated Abs were then incubated for 1 hour at 37°C in 5% FBS in PBS ...
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bioRxiv - Immunology 2021Quote: ... HRP-conjugated IgM or IgG (Southern Biotech) was incubated at 37 °C for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... APS conjugated IgM and IgG (Southern Biotech) were added and incubated for 1 hour at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Normal rabbit IgG (SouthernBiotech, Cat# 0111-01) was used as a negative control ...
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bioRxiv - Molecular Biology 2019Quote: ... or 10 μg rabbit IgG (Southern Biotech) overnight ...
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bioRxiv - Immunology 2023Quote: ... Human IgG (cat# 0150-01, Southern Biotech) + Human IgM-L (cat# 0158L-01 ...
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bioRxiv - Microbiology 2023Quote: ... and individual IgG subclasses 1-4 (SouthernBiotech) were diluted to manufacturer recommended concentrations and incubated for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... and then incubation for 30 mins at room temperature with anti-human IgG HRP at 1:4000 (Southern Biotech #2040-05). 5 washes of TBST were done followed by development using 1-Step Ultra TMB-ELISA Substrate Solution (ThermoFisher Scientific ...