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Citations for SouthernBiotech :
801 - 832 of 832 citations for Son of sevenless homolog 1 SOS1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... anti-IgG2 Fc-Alexa Fluor 488 and mouse anti-IgG3 hinge-Alexa Fluor 647 (1 µg/ml, all from Southern biotech) for an additional 20 minutes at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... diluted 1:1000 in PBST in order to detect the primary antibody for 1 h at room temperature before being mounted on glass slides and coverslipped with Fluoromount-G (0100-01, Southern Biotech). Images of cresyl violet stained sections were obtained using an Axio Observer Carl Zeiss light microscope whereas fluorescent images from immunolabelled sections were collected using an Axio Observer Carl Zeiss fluorescent microscope ...
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bioRxiv - Immunology 2022Quote: ... at 37 °C for 1 hr followed by the multi-species SP ads-HRP-conjugated goat anti-mouse IgG (H+L) antiserum (Southern Biotech) diluted at 1:20.000 and incubated at 37 °C for 1 hr ...
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bioRxiv - Physiology 2023Quote: ... Non-specific antibody binding was blocked for 1 hour at room temperature with a blocking solution containing: 5% normal goat serum (006001, SouthernBiotech™), 0.3% Triton™ X-100 (X100 ...
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bioRxiv - Biochemistry 2023Quote: ... then washed and secondary antibody added at 1:2000 or 1:4000 dilution in blocking buffer (100□μl/well) using either anti-mouse IgG-HRP (Catalog #1030-05, Southern Biotech), or anti-mouse IgA-biotin (Catalog #1040-80 ...
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bioRxiv - Immunology 2024Quote: ... diluted to 1 μg/ml in T-PBS followed by incubation for 1 hour with goat-anti mouse IgG conjugated with HRP (Southern Biotech) diluted 2000 × in T-PBS.
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bioRxiv - Immunology 2024Quote: ... diluted to 1 μg/ml in T-PBS followed by incubation for 1 hour with goat-anti mouse IgG conjugated with HRP (Southern Biotech) diluted 2000 × in T-PBS ...
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bioRxiv - Immunology 2024Quote: ... Specific IgG was detected for 1 hour at room temperature with biotin rat anti-mouse IgG (Southern Biotech, Birmingham, AL, USA) biotin rat anti-mouse IgG1 (Southern Biotech) ...
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bioRxiv - Immunology 2024Quote: ... samples were incubated with 100 μl of a 1:500 diluted phycoerythrin (PE)-tagged goat-anti mouse IgG-Fc (Southern Biotech) in the dark at RT with shaking at 600 rpm for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... Wells then were incubated for 1 hour with horseradish peroxidase (HRP)-conjugated goat anti-human IgG (monkey pre-adsorbed) (Southern Biotech) at a 1:4,000 dilution ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed with ELISA wash buffer then incubated for 1 h at RT with an HRP conjugated goat anti-mouse (Southern Biotech). Plates were washed with ELISA wash buffer and developed using 1-Step Ultra TMB-ELISA substrate solution (Thermo Fisher) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed 3x with DPBS and coverslips were mounted on 1 mm glass slides using Fluoromount-G (SouthernBiotech, 0100-01).
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bioRxiv - Molecular Biology 2022Quote: ... once with 0.01X Wash Buffer B for 1 min at room temperature and coverslips were mounted on the slide with DAPI Fluoromount-G mounting medium (SouthernBiotech, Birmingham, USA).
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bioRxiv - Immunology 2022Quote: ... plates were washed 4× with ELISA wash buffer and a 1:4000 dilution of goat anti-mouse IgG (SouthernBiotech, catalog #1030-05) in ELISA blocking buffer was added to each well and incubated at 37°C for 1 hour followed by 4 washes with ELISA wash buffer ...
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bioRxiv - Microbiology 2022Quote: ... the beads were incubated with 1 µg/mL Alexa Fluor647-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech, 2062-31) in PBS-TH for 30 min at 4 °C with shaking ...
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bioRxiv - Biochemistry 2022Quote: ... and then treated with alkaline phosphatase (AP)-conjugated goat anti-rabbit-IgG (Southern Biotech; 1:8000 in TBST with 2% (w/v) bovine serum albumin) ...
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bioRxiv - Immunology 2020Quote: ... plates were coated with 1:2000 (for capture of polyclonal macaque IgG) or 1:1000 (for capture of polyclonal human IgG) of goat anti-Human IgG λ and K (Southern Biotech). The next day ...
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bioRxiv - Biochemistry 2020Quote: ... for 1 min, washed with PBS again (3X, 5 min) and slides were mounted with Fluoromount-G mounting medium (SouthernBiotech, Birmingham, AL). Images were acquired on a Leica SP5 confocal microscope and analyzed by Fiji ImageJ.
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bioRxiv - Immunology 2024Quote: ... Plates were then washed 3x with wash buffer and incubated with a 1:4,000 dilution (in EZ block) of Goat Anti-Mouse IgG Fc-HRP secondary (Southern Biotech, 1033-05) for 1 hr at RT ...
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bioRxiv - Immunology 2024Quote: ... Plates were subsequently washed three times prior to the addition of streptavidin-alkaline phosphatase (1:2000, Southern Biotech, Birmingham, AL, United States) to all wells for 1 hour at 37°C ...
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bioRxiv - Immunology 2020Quote: ... the plates were first washed 5 times with PBS-T and then incubated for 1 hour at RT with HRP-conjugated anti-human IgG (Fc-specific, clone JDC-10, Southern Biotech, Birmingham, AL) diluted 3000 × in PBS-T ...
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bioRxiv - Immunology 2020Quote: ... the plates were washed at least 5 times with PBS-T and then incubated for 1 hour at RT with HRP-conjugated anti-human IgG (Fc-specific, clone JDC-10, Southern Biotech, Birmingham, AL) diluted 3000 × in PBS-T ...
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bioRxiv - Immunology 2023Quote: ... serum samples were applied in a 1:100 dilution and concentrations were determined from an IgG1 or IgG2a standard curve (Southern Biotech, Birmingham, USA). Horseradish peroxidase-linked goat anti-mouse IgG ...
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bioRxiv - Microbiology 2022Quote: ... PrPSc on membranes was detected using mAb PRC5 at a dilution of 1:5000 followed by alkaline phosphatase conjugated goat anti mouse IgG (Southern Biotech, 1030-04) at a dilution of 1:5000 ...
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bioRxiv - Immunology 2022Quote: ... cells were washed twice with FACS buffer and incubated for 30 min at 4°C with 1:1000 diluted PE-conjugated goat F(ab)’2 anti-human IgG (Southern Biotech 2042-09) in the dark ...
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bioRxiv - Immunology 2022Quote: ... and the cells were stained with 50 μL/well of a 1:1,000 dilution of goat anti-human IgG-PE (Southern Biotech, cat. # 2040-09) secondary antibodies for 30 min at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Plates were then incubated with a 1:1000 dilution of mouse serum raised against ZE3-6H followed by goat anti-mouse IgG HRP conjugate (Southern Biotech, Birmingham, AL, USA) for 1 h at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... They were washed six times in washing buffer (PBS with 0.1% Triton X-100) for 20 min and blocked for 1 h in washing buffer containing 5% heat-inactivated goat serum (SouthernBiotech, Cat. No. 0060-01), followed by incubation with the primary antibodies (rabbit anti–GFP ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were incubated for 60 min in blocking buffer (TBS-LI-COR, Biosciences #927-60001; supplemented with 1% Goat Serum, Southern Biotech #00060-01). Fixed cells were then incubated with primary (ON ...
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bioRxiv - Immunology 2024Quote: ... plates were subsequently washed and incubated with 0.1 mL per well of goat anti-human IgG horseradish peroxidase (HRP; Southern Biotech; 2030-05, 1:5000), for 1 hour at 37°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Plates were incubated with serially diluted serum or gut content (50 μL, PBS 1% BSA, wt/vol, 2 h, RT) and standard mouse IgA (Southern Biotech #0106-01, prepared in duplicates) and washed (5x ...
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bioRxiv - Immunology 2020Quote: ... Plates were incubated with serially diluted mouse sera (50 µL, PBS 1% BSA, wt/vol, 2 h, RT) and standard mouse IgG (Southern Biotech #0107-10, prepared in duplicates) and washed (5x ...