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Citations for SouthernBiotech :
551 - 600 of 832 citations for Son of sevenless homolog 1 SOS1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Isotype analysis was perfomed by incubating the immune complexes with secondary goat anti-mouse-PE antibody (IgG1 1070-09, IgG2a 1080-09S, IgG2b 1090-09S, IgG3 1100-09, IgM 1020-09, IgA 1040-09 Southern Biotech) for each isotype ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed 4X in wash buffer and the secondary antibody Goat anti-Human Ig-HRP (Southern Biotech, Cat# 2010-05), Peroxidase-conjugated AffiniPure Donkey Anti-Human IgG ...
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bioRxiv - Immunology 2020Quote: ... Serum was subsequently added and antibodies were detected using goat anti-mouse IgG1 or goat anti-mouse IgG2c conjugated to HRP (Southern Biotech) and 1xTMB (Thermofisher ...
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bioRxiv - Immunology 2021Quote: ... Bound IgA or IgM antibody was detected by incubation with anti-IgA-alkaline phosphatase (goat anti-mouse IgA (α chain-specific) (SouthernBiotech) or anti-IgM-alkaline phosphatase goat anti-mouse IgM (μ chain-specific ...
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bioRxiv - Immunology 2021Quote: ... buffer (PBS with 0.1% bovine serum albumin and 0.1% sodium azide) containing the PE-labeled tetrameric pBF2*1501 complex and fluorescein isothiocyanate (FITC)-labeled anti-CD8 monoclonal antibody (SouthernBiotech, USA) for 30 min at 4°C ...
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bioRxiv - Immunology 2020Quote: An ELISA to detect anti-CCHFV Ig was performed as previously described (Hawman et al., 2019) with an anti-mouse Ig detection antibody (Southern Biotech) to detect all immunoglobulin isotypes or anti-mouse IgG or IgM (Southern Biotech ...
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bioRxiv - Immunology 2020Quote: ... followed by addition of HRP-conjugated goat anti-mouse IgG second antibody (catalog number 1030-05, Southern Biotech, Birmingham, AL, USA). TMB substrate was added and the plates read at OD 450 nm with a plate reader ...
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bioRxiv - Immunology 2021Quote: ... and antigen-bound antibodies were detected by using a PE-coupled detection antibody for each subclass and isotype (IgG1, IgG2, IgG3, IgG4, IgA1, and IgM; Southern Biotech), and Fcγ-receptors were fluorescently labeled with PE before addition to immune complexes (FcγR2A ...
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bioRxiv - Cell Biology 2020Quote: ... localization of antibody labeling was detected after a 1 hr incubation with secondary antibody diluted in PBS containing 2% normal goat serum and mounting under a coverslip with DAPI Fluoromount-G (Southern Biotech). PPCs were visualized by fluorescent microscopy and their numbers were assessed qualitatively by visual examination ...
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bioRxiv - Neuroscience 2020Quote: ... After thorough washing fluorescent secondary antibodies were applied at room temperature for 3 hours before counterstaining with DAPI and mounting with FluoroMount G (Southern Biotech). Slides were imaged using a Leica SP8 using 20x (NA 0.75 ...
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bioRxiv - Immunology 2023Quote: ... Sections were washed in PBS before incubation with secondary antibodies or labelled streptavidin (Supplementary Table 4) for 1hr at room temperature followed by mounting with DAPI containing fluoromount (Southern Biotech). Small to medium sized airways in lung sections were captured by an investigator blind to sample identity with an EVOS FL imaging system (Thermo) ...
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bioRxiv - Immunology 2023Quote: ... was added to the wells for 1h followed by washing and incubation with horseradish peroxidase-linked goat anti-mouse IgG secondary antibody (Southern Biotech). The avidity index was calculated as ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were washed with TBS-T 0.1% three times and incubated for one hr at room temperature with goat anti-mouse Ig horseradish peroxidase (HRP)-conjugated antibodies (Southern Biotech, anti-IgM secondary 1:1000 (cat# 1020-05) ...
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bioRxiv - Immunology 2023Quote: ... The plates were washed and incubated with a peroxidase-conjugated goat anti-mouse anti-IgM or anti-IgG antibody (Southern Biotech). After washing ...
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bioRxiv - Microbiology 2023Quote: ... 1×109 bacteria were washed with 1 ml ice-cold PBS and stained for 30 min at 4°C with 4 µg of FITC-conjugated anti-mouse IgA antibody (Southern Biotech) in 100 µl of staining buffer ...
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bioRxiv - Microbiology 2024Quote: ... Concentrations were evaluated by linear regression analysis built by potting OD405 values against a standard curve generated by titration of a human IgG-unlabeled antibody (Southern Biotech).
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bioRxiv - Immunology 2022Quote: ... Hp-specific IgG1 was detected as previously described47 using plates coated with Hp extract and detected using rat anti-mouse IgG1-HRP antibody (Southern Biotech). NP-OVA specific IgG1 was detected as previously described48 using plates were coated with TNP(5)-BSA and detected using goat anti-mouse IgG1-HRP (Southern Biotech).
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bioRxiv - Immunology 2022Quote: ... The microtiter plates were developed with horseradish peroxidase (HRP)-labeled goat anti-human IgG or IgM polyclonal antibodies (SouthernBiotech, Birmingham, AL). The plates were developed with 3,3 ...
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bioRxiv - Immunology 2022Quote: ... The plates were then washed three times with water and 200 μl of goat anti-human IgG HRP secondary antibody (Southern Biotech) diluted to a ratio of 1:2000 in block buffer was added and incubated for 1 hr at room temperature followed by an hour of incubation ...
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bioRxiv - Cell Biology 2023Quote: ... Surface-bound sLAP was detected with anti-human PE antibodies that bound directly to the Fc-IgG part of the chimeric sLAP proteins (SouthernBiotech, USA). PE signals were analyzed with FlowJo software (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... NP-specific IgM and IgG3 antibodies were detected using biotinylated anti-mouse IgM- or IgG3-specific immunoglobulins followed by streptavidin-HRP (Southern Biotech) and developed with SIGMAFAST OPD tablets (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... and antigen-bound antibodies were detected by using a PE-coupled detection antibody for each subclass and isotype (IgG1, IgG2, IgG3, IgA1, IgA2 and IgM; Southern Biotech), and Fcγ-receptors were fluorescently labeled with PE before addition to immune complexes (FcγR2A ...
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bioRxiv - Microbiology 2024Quote: ... IgG3 and IgA antibodies from serum and vaginal lavage were measured by standard ELISA(25) using a BioMek i7 liquid handler and isotype-specific detection antibodies from Southern Biotech. Results were reported as endpoint titer or EC50 of a 4PL curve fit using GraphPad Prism software (GraphPad Software ...
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bioRxiv - Immunology 2024Quote: ... and antigen-bound antibodies were detected by using a PE-coupled detection antibody for each subclass and isotype (IgG1, IgG2, IgG3, IgA1, IgA2 and IgM; Southern Biotech), and Fc-receptors were fluorescently labeled with PE before addition to immune complexes (FcγR2A ...
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bioRxiv - Immunology 2024Quote: ... IgG and IgA classes were detected using the anti-human isotype specific alkaline phosphatase (AP)-conjugated goat anti-human secondary antibodies (Southern Biotech). Following extensive washing ...
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bioRxiv - Immunology 2024Quote: ... followed by 2x washes with PBS + 0.1% BSA and by incubation with a mouse anti-human IgG Fc-PE secondary antibody (SouthernBiotech 9040-09) at 1 µg/mL for 20-30 minutes at 4°C with shaking at 900 rpm ...
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bioRxiv - Immunology 2024Quote: ... Secondary antibody staining was performed with goat a-rabbit (4050–05) or goat a-mouse-HRP (1031-05, both Southern Biotech).
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bioRxiv - Immunology 2024Quote: ... The ELISPOT plate was washed five times with PBS and anti-mouse IgG1 or anti-mouse IgG2c antibodies conjugated with alkaline phosphatase (Southern Biotech) at 1:1000 in PBS were added and incubated at room temperature for 2 hours ...
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bioRxiv - Genetics 2021Quote: ... Wells were washed as described and biotin conjugated goat anti-human IgG (Jackson AffiniPure #109-065-098; 1:30,000 or Southern Biotech 2049-08; 1:1,000) in PBS/1.0% BSA detection antibody was added to the wells at 100ul/well and incubated for 2 hours at room temperature with shaking ...
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bioRxiv - Immunology 2022Quote: ... To detect S-specific hamster IgG, goat anti-hamster IgG (1:4000, Novus, Cat# NB1207141) plus HRP-conjugated rabbit anti-goat IgG (1:5000, Southern Biotech, Cat# 61-6065) were used ...
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bioRxiv - Immunology 2022Quote: ... or 1:1000 diluted goat anti-human IgM-HRP (SouthernBiotech) was added to each well and plates were incubated for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... HRP goat anti-mouse IgG (1:10,000 SouthernBiotech 1031-05) and HRP goat anti-rabbit IgG (1:10,000 SouthernBiotech 4030-05).
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bioRxiv - Immunology 2022Quote: ... or goat anti-hamster IgG-HRP (1:32,000, Southern Biotech) was added for 1 hour at 37 °C ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 min and Fluoromount-G (Southern Biotech, 0100-01).
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bioRxiv - Immunology 2021Quote: ... or 1:1000 diluted goat anti-human IgM-HRP (SouthernBiotech) was added to each well and plates were incubated for 1 hour ...
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bioRxiv - Cancer Biology 2021Quote: ... goat anti-rabbit (Southern Biotech; 4030-05, RRID:AB_2687483, 1:10000). Deltarasin (CAS #1440898-82-7 ...
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bioRxiv - Microbiology 2022Quote: ... Normal rabbit IgG (Southern Biotech, Cat# 0111-01, 1:100) was used as a negative control ...
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bioRxiv - Microbiology 2023Quote: ... Normal rabbit IgG (Southern Biotech, Cat# 0111-01, 1:100) was used as a negative control ...
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bioRxiv - Immunology 2023Quote: ... anti-mouse IgG (SouthernBiotech, Catalog 1080-05, used 1/5000) antibody was added and incubated at 37°C for 1 h ...
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bioRxiv - Microbiology 2024Quote: ... and goat-anti rabbit IgG-HRP (SouthernBiotech; dilution: 1:10‘000)
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bioRxiv - Microbiology 2023Quote: ... Normal rabbit IgG (Southern Biotech, Cat# 0111-01, 1:100) was used as a negative control ...
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bioRxiv - Developmental Biology 2023Quote: ... or anti-mouse (SouthernBiotech, 1036-05, 1:2000, RRID: AB_2794348) secondary antibodies and visualized using a MicroChemi (DNR Bio Imaging Systems ...
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bioRxiv - Microbiology 2022Quote: ... Normal rabbit IgG (Southern Biotech, Cat# 0111-01, 1:100) was used as a negative control ...
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bioRxiv - Microbiology 2024Quote: ... or goat-anti-mouse IgG (PO447, Southern Biotech, 1:100) in PBS/0,1% BSA for 1 hour at RT ...
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bioRxiv - Cell Biology 2024Quote: ... donkey anti-rabbit IgG AF-488 1:1000 (Southern Biotech), donkey anti-mouse IgG AF-568 1:1000 (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... donkey anti-mouse IgG AF-488 1:1000 (Southern Biotech), donkey anti-rabbit IgG AF-488 1:1000 (Southern Biotech) ...
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bioRxiv - Microbiology 2020Quote: ... Samples were stained with primary antibodies specific to dsRNA (see Table S8) as well as DAPI (DAPI Fluoromount-G, SouthernBiotech, 0100-20) to visualize the nuclei using a Nikon Eclipse Ti microscope (Nikon ...
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bioRxiv - Bioengineering 2022Quote: ... We washed with PBS three times to remove the unbound fractions and treated the mixtures with anti-human IgG secondary antibody (SouthernBiotech #2016-05). The plate was further incubated at room temperature for 30 min ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed with 0.05% Tween-20 in PBS and further incubated with HRP-conjugated goat antibodies directed against mouse IgG (Southern Biotech, Birmingham, AL) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... washed 3 times in ice-cold PBSM and then bound antibody detected with goat anti-human kappa-A647 (SouthernBiotech, cat# 2060-31) After washing ...