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Citations for SouthernBiotech :
751 - 800 of 868 citations for Integrin beta 1 binding protein 1 ITGB1BP1 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Antibody subtype identification was performed using the SBA ClonotypingTM System/HRP Kit (Southern Biotech, Birmingham, AL, USA). To minimize the background impurities observed during immunoelectron microscopy ...
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bioRxiv - Immunology 2022Quote: ... Nunc Immuno Plate MaxiSorp plates were coated with 5 μg/ml anti–Ig capture antibody (Southern biotech) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... plates were incubated with horseradish peroxidase (HRP)-conjugated anti-human IgG secondary antibody (Southern Biotech, 2042-05) diluted 1:4000 in PBS containing 0.05% Tween 20 and 1% BSA for 45 min at RT ...
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bioRxiv - Synthetic Biology 2024Quote: ... the cells were incubated with an anti-human IgG antibody conjugated to Alexa Fluor-488 (Southern Biotech Catalog# 2015-30 ...
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bioRxiv - Microbiology 2020Quote: ... Bound antibodies were detected with horseradish peroxidase (HRP)-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech) in 1% BSA in PBS-T and Tetramethylbenzidine (TMB ...
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bioRxiv - Immunology 2021Quote: ... To generate a standard curve a dilution series using the Monkey IgG-UNLB antibody (Southern Biotech, 0135-01) was coated on each plate and incubated overnight at 4°C ...
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bioRxiv - Biophysics 2020Quote: The anti-mouse-Igκ antibody for clustering BCRs was purchased from Southern Biotech (Birmingham, AL; catalogue #1050-01). AF647-conjugated anti-mouse-IgM Fab fragments (catalogue #115-607-020 ...
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bioRxiv - Biochemistry 2021Quote: ... 500 μL of a secondary antibody solution (5 μM Alexa Fluor® 488 anti-mouse (SouthernBiotech; 1031-30) and 10 μM Alexa Fluor® 647 anti-human (SouthernBiotech ...
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bioRxiv - Immunology 2020Quote: ... Detection was performed using an HRP-linked goat anti-rabbit or goat anti-mouse secondary antibody (Southern Biotech) and chemiluminescence detection (ECL Plus™ ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed three times and incubated with HRP-conjugated goat anti-human secondary antibody (ITK Southern Biotech) diluted 1:2000 in blocking buffer for 1 hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... the bound antibodies were detected with alkaline phosphatase-conjugated isotype-specific goat anti-mouse polyclonal serum (Southern Biotech).
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bioRxiv - Immunology 2021Quote: ... and then incubated with either goat anti-human IgG-HRP conjugated antibodies (Southern Biotech, catalog no. 2040-05), goat anti-human IgA-HRP conjugated antibodies (Southern Biotech ...
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bioRxiv - Immunology 2020Quote: ... Cells were then surface stained with S/RBD probes and the following antibodies: IgD AF488 (polyclonal; Southern Biotech), IgM BUV395 (G20-127) ...
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bioRxiv - Immunology 2023Quote: ... and incubated with 0.75x serial dilutions of purified polyclonal human antibodies (IgG, SouthernBiotech, Cat # 0150-01; IgM, SouthernBiotech, Cat # 0158L-01 ...
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bioRxiv - Biochemistry 2023Quote: ... incubated with an appropriate peroxidase-labeled secondary antibody (anti-mouse IgG1-HRP #1070-05, Southern Biotech, Birmingham, USA) for 30 min ...
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bioRxiv - Immunology 2024Quote: ... IgG3 and IgA levels were detected by using HRP-coupled goat anti-mouse subtype-specific secondary antibodies (SouthernBiotech).
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bioRxiv - Immunology 2023Quote: ... ELISA antibodies for measuring total and HDM-specific Igs in serum were purchased from Southern Biotech (Birmingham, AL). ELISA data were analyzed using SoftMax Pro software (Molecular Devices).
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bioRxiv - Immunology 2022Quote: ... Plates were washed 3x with PBS and treated with an AP conjugated anti-mouse IgG antibody (Southern Biotech) for 1.5 hours ...
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bioRxiv - Genetics 2022Quote: ... Plates were washed using ELISA wash buffer and incubated with HRP-conjugated anti-Ig secondary antibodies (Southern Biotech) for 2 hours in humid storage at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... After secondary antibody incubation slides were washed in PBS and mounted with FluoromountG with DAPI (SouthernBiotech 0100-20). For immunolabeling of NaVs the following primary antibodies were used ...
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bioRxiv - Immunology 2021Quote: ... Tests were performed in triplicates and with a positive control (anti-CD3 monoclonal antibody (0,05 μg/ml; Southern Biotech). PVDF backed microplate plates pre-coated with IFNγ-specific antibodies (R&D systems ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed three times with PBS-T and secondary goat anti-mouse IgG-HRP antibody (Southern Biotech), diluted 1:4000 in ELISA buffer ...
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bioRxiv - Immunology 2021Quote: ... and permeabilized prior to staining with a FITC-conjugated goat anti-rhesus IgG (H+L) polyclonal antibody (Southern Biotech). Data represent the frequency of cells positive for IgG-binding to Env for post-vaccination samples compared to the pre-vaccination sample ...
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bioRxiv - Immunology 2022Quote: ... Plates were then washed three times with PBS-T and secondary goat anti-mouse IgG-HRP antibody (Southern Biotech), diluted 1:4000 in ELISA buffer ...
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bioRxiv - Immunology 2022Quote: ... cryopreserved PBMC were thawed and stained with the following antibodies (monoclonal unless indicated): IgD FITC (goat polyclonal, Southern Biotech), IgM PerCP-Cy5.5 (clone G20-127 ...
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bioRxiv - Immunology 2021Quote: ... For the three-step protocol sections were stained using a goat anti-mouse IgG1-HRP-conjugated antibody (Southern Biotech) for CXCL12 and goat anti-mouse IgG2b-HRP-conjugated antibody (Southern Biotech ...
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bioRxiv - Immunology 2021Quote: ... 96 well plates (Costar) were incubated with anti-IgM or anti-IgG capturing antibody at 1ug/ml (Southern Biotech) and incubated at 4°C for 18h ...
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bioRxiv - Immunology 2021Quote: ... The cells were washed with FACS buffer and incubated with secondary goat anti-human IgG PE antibody (Southern Biotech) for 1 hour at 4°C ...
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bioRxiv - Immunology 2021Quote: The IgG subclass profile in serum samples was analyzed using IgG subclass-specific secondary antibodies (Southern Biotech, Birmingham, AL), and the ratios of IgG2a/IgG1 and IgG2b/IgG1 were calculated using the MFI readouts at the serum dilution (1:2000 ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibodies used in this study were: horseradish peroxidase (HRP)-conjugated goat anti-rat IgG (Southern Biotech 3010-05), HRP-conjugated goat anti-mouse IgG (Southern Biotech 1010-05) ...
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bioRxiv - Immunology 2023Quote: ... The IgG assay contained a goat anti-mouse IgG Fc antibody (SouthernBiotech, 1033-31, labeled with Alexa Fluor 488) and the IgM assay a donkey anti-mouse IgM F(ab’)2 (Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were stained again for 30 min with 0.5 µg of FITC-conjugated anti-mouse IgA antibody (Southern Biotech) in 100 µl of staining buffer to refresh and harmonize the staining between batches ...
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bioRxiv - Cell Biology 2024Quote: Secondary antibodies for immunoblotting were horseradish peroxidase (HRP)-conjugated goat anti-rat immunoglobulin (Ig) (Southern BioTech Cat#3010-05), HRP-conjugated goat anti-rabbit Ig (Southern BioTech Cat#4010-05 ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were washed three times with PBSTw and incubated for three hours with appropriate HRP-conjugated secondary antibody (SouthernBiotech) at room temperature ...
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bioRxiv - Immunology 2023Quote: ... cryopreserved PBMC were thawed and stained with the following antibodies (monoclonal unless indicated): IgD FITC (goat polyclonal, Southern Biotech), IgM PerCP-Cy5.5 (clone G20-127 ...
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bioRxiv - Bioengineering 2024Quote: ... plates were washed with PBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG1 antibodies (Southern Biotech) or HRP-conjugated goat anti-mouse IgG2a-HRP antibodies (Southern Biotech ...
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bioRxiv - Synthetic Biology 2024Quote: ... The blot was then incubated for 1 h at room temperature with 0.5 µg/mL of goat anti-human kappa antibody conjugated to HRP (31460, SouthernBiotech) if hSC44.20 ...
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bioRxiv - Genetics 2021Quote: ... Primary antibodies (Goat Anti-Type I Collagen, 1310-01, Southern Biotech; Goat Anti-Type V Collagen, 1350-01, Southern Biotech) were diluted 1:100 in 4% DS in TBST and incubated overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 × 105 dendritic cells or B cells were incubated with different concentrations of C03 with or without goat anti-human Fc antibody (SouthernBiotech) for 48 hours ...
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bioRxiv - Immunology 2021Quote: ... the same ELISA was performed but using the secondary antibodies from SBA Clonotyping System-HRP kit (SouthernBiotech, 5300-05, RRID:AB_2796080) diluted accordingly to manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... at 1×106 cells/well and stimulated for 48 hours with 5 μg/mL of RBD-His or hCD81-His or media alone (RPMI + 5% FCS) in the presence of anti-mouse CD28 antibody [0,5 μg/ml] (Southern Biotech). As positive control cells were stimulated with anti-mouse CD3 [0,05 μg/ml] (Southern Biotech) ...
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bioRxiv - Immunology 2020Quote: ... plates were washed 5 times with washing buffer and incubated with goat-anti-human IgG or goat-anti-human IgG(H+L) secondary antibody conjugated to horseradish peroxidase (HRP) (SouthernBiotech) in blocking buffer at a 1:4000 or 1:2000 dilution ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed three times and incubated with horseradish peroxidase (HRP)-conjugated goat anti-human secondary antibody (ITK Southern Biotech) diluted 1:2000 in blocking buffer for 1 hour at RT ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 5 times with PBS-T and incubated with goat anti-mouse IgG1 antibody conjugated with horseradish peroxidase (SouthernBiotech) for 1 hour at room temperature ...
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bioRxiv - Immunology 2023Quote: ... antibody response to individual peptides was detected with different fluorescently labeled secondary antibodies (Goat anti-human IgM-AF488, SouthernBiotech, Cat # 2020-30; Goat anti-human IgG-PE, SouthernBiotech, Cat # 2040-09 ...
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bioRxiv - Immunology 2023Quote: Biotinylated Goat F(ab)2 anti-human isotype antibodies (anti-human IgM, SouthernBiotech, Cat # 2022-01; anti-human IgA, SouthernBiotech, Cat # 2052-01 ...
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bioRxiv - Immunology 2023Quote: ... plates were washed in wash buffer (PBS-0.05%Tween 20) and incubated with AP-conjugated secondary antibodies (anti-IgM, anti-IgG1, Southern Biotech). Plates were washed and followed by adding alkaline phosphatase (AP) ...
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bioRxiv - Microbiology 2024Quote: ... Antibodies used for flow cytometry analysis performed in this study were: FITC-conjugated anti-human IgM (Southern Biotech #2020-02), goat anti-human IgG-UNLB (Southern Biotech #2040-01) ...
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bioRxiv - Microbiology 2023Quote: ... Bound antibodies were detected by using goat anti-mouse IgG conjugated to horseradish peroxidase (HRP; Southern Biotech, Birmingham, AL, USA) diluted 1:2000 in PBST and developed using 3,3’,5,5’-tetramethylbenzidine substrate (Thermo Scientific Pierce ...
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bioRxiv - Microbiology 2023Quote: ... was used except that plates were coated with anti-human kappa and lambda light chain specific mouse antibodies (Southern Biotech) at 1:1 ratio diluted to 1 μg/ml in PBS overnight ...