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Citations for SouthernBiotech :
501 - 550 of 868 citations for Integrin beta 1 binding protein 1 ITGB1BP1 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... wells were washed six times with PBS supplemented with 0.1 % Tween20 and incubated for 2 h with 1 μg/mL biotinylated goat α-mouse IgM or IgG1 (SouthernBiotech) at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... Membranes were incubated in TBST containing 5% (w/v) skimmed milk with goat anti-mouse IgG conjugated to horseradish peroxidase (HRP) (1:2000, 1010-05, SouthernBiotech) for 3h at 4°C in a rotary platform and washed thrice with TBST for 10 min each time ...
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bioRxiv - Cell Biology 2023Quote: ... All coverslips were then washed 3 times in PBS before being mounted on a drop of mounting medium (DAPI fluoromount – G 1:1000; SouthernBiotech)) on labeled glass coverslips ...
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bioRxiv - Molecular Biology 2023Quote: ... 0,1 % BSA and washed three times with 1× PBS before mounting on microscope slides using Fluoromount-G (Cat# 0100-01, SouthernBiotech,). Hoechst 33342 dye (Cat# B2261 ...
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bioRxiv - Bioengineering 2023Quote: ... The staining procedure involved the following steps: (1) The sections were blocked in a solution of 10% normal goat serum (SouthernBiotech) and 0.3% Triton-X in PBS for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 × 105 cells in 100 μL per well were plated in a 96-well plate pre-coated with anti-mouse CD3ε (1 μg/mL, SouthernBiotech). Tumors were harvested from mice 10 days post inoculation and dissociated using gentleMACS (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... 25 μl of undiluted supernatant was added to wells coated with 1 μg/ml goat anti-mouse Ig (Southern Biotech) or HAPR8 and for serum samples 3-fold serial dilutions of serum samples starting at 1/100 were added to wells coated with NP4-BSA (10 μg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then stained with iamidino-2-phenylindole (DAPI; 1:10000) to label nuclei and mounted in Fluoromount-G Mounting Medium (SouthernBiotech). Images of whole sections were acquired with a Zeiss Axio ScanZ1 slide scanner using a 20x objective lens and Zen Blue 3.1 software (Carl Zeiss ...
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bioRxiv - Neuroscience 2024Quote: ... the tissue was allowed to dry on the slides at 4 °C and then briefly re-wet with 1 M PBS before being covered with DAPI Fluoromount-G (SouthernBiotech) and coverslipped ...
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bioRxiv - Immunology 2021Quote: ... The secondary antibody used was horseradish-peroxidase (HRP)-conjugated goat polyclonal antibody against mouse IgG1 (1070-05; Southern Biotech, Cambridge, United Kingdom), HRP-conjugated goat polyclonal antibody against rabbit IgG (4050-05 ...
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bioRxiv - Immunology 2021Quote: ... An HRP-conjugated goat anti-mouse IgE antibody (SouthernBiotech) was used for detection ...
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bioRxiv - Biochemistry 2021Quote: ... Secondary antibodies (Alexa Fluor® 488 (SouthernBiotech; 1031-30); Alexa Fluor® 647(SouthernBiotech ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies [either anti-mouse (Southern Biotech 1031-05) or a 1:1 mix of anti-rabbit (Southern Biotech ...
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bioRxiv - Microbiology 2020Quote: ... or goat anti-mouse-HRP antibody (1010-05, SouthernBiotech). Then the membrane was washed three more times for 15 minutes in TBST buffer ...
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bioRxiv - Systems Biology 2021Quote: ... and a horseradish peroxidase-conjugated secondary antibody (Southern Biotech) were used to probe membranes ...
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bioRxiv - Immunology 2022Quote: ... and IgA-HRP detection antibodies (Southern Biotech, AL, USA) were added for another hour ...
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bioRxiv - Immunology 2022Quote: ... Appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies (Southern Biotech) were added and incubated for 1 hr followed by a colorimetric detection assay utilizing 3,3′,5′,5-tetramethylbenzidine (TMB ...
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bioRxiv - Microbiology 2022Quote: ... goat anti-rabbit and rabbit anti-goat antibodies (SouthernBiotech) were used as secondary antibodies with a dilution of 1:2,000.
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bioRxiv - Cancer Biology 2020Quote: ... and an anti-mouse/rabbit secondary antibody (Southern Biotech). Protein bands were visualised by Western HRP substrate (Millipore ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by peroxidase-labeled secondary antibody (SouthernBiotech, 1010-05). Membranes were then stripped and probed with anti-beta actin (Abcam) ...
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bioRxiv - Immunology 2021Quote: ... goat anti-human IgA-HRP conjugated antibodies (Southern Biotech), or goat anti-human IgM-HRP conjugated antibodies (Southern Biotech ...
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bioRxiv - Immunology 2021Quote: ... PE-coupled mouse anti-human detection antibodies (Southern Biotech) were used to detect antigen-specific antibody binding ...
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bioRxiv - Immunology 2020Quote: Anti-Human IgG Polyclonal Antibody (Southern Biotech 2040-01) was immobilized via amine group on two flow cells of a CM5 sensor chip ...
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bioRxiv - Bioengineering 2022Quote: ... 5 µL of blocking antibody (anti-IgG, Southern Biotech) was added to the 50 µL cell solution to yield a final concentration of 25 µg/mL ...
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bioRxiv - Immunology 2022Quote: ... Goat anti-human IgG Fc secondary antibody (Southern Biotech) diluted in block buffer (1:4000 ...
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bioRxiv - Immunology 2024Quote: ... mouse monoclonal anti-matrix antibody (Southern Biotech, Birmingham, AL), was diluted 1:500 and added to the cells in 1% BSA for 1 hour at RT ...
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bioRxiv - Immunology 2024Quote: ... goat anti-mouse IgG2b HRP antibody (#1091-05, SouthernBiotech), goat anti-mouse IgG2c HRP antibody (#1078-05 ...
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bioRxiv - Immunology 2024Quote: ... goat anti-mouse IgG2c HRP antibody (#1078-05, SouthernBiotech), and goat anti-mouse IgG3 HRP antibody (#1101-05 ...
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bioRxiv - Immunology 2024Quote: ... goat anti-mouse IgG2a HRP antibody (#1081-05, SouthernBiotech), goat anti-mouse IgG2b HRP antibody (#1091-05 ...
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bioRxiv - Bioengineering 2024Quote: ... Peroxidase-labelled anti-IgE antibody (#9160-05, Southern Biotech) was incubated (1/1000 ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies [either anti-mouse (Southern Biotech; 1031-05), anti-sheep (Novus NBP1-73267 ...
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bioRxiv - Immunology 2023Quote: ... anti-human HRP-conjugated goat secondary antibody (Southern Biotech) was added at 20,000x at 50 μl per well and incubated for 30 minutes.
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies Goat anti-mouse IgG1-HRP conjugate (SouthernBiotech) diluted 1:2500 ...
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bioRxiv - Cell Biology 2023Quote: ... Antibodies against Type I collagen (Southern Biotech, as above), MRC2 (R&D ...
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bioRxiv - Microbiology 2024Quote: ... Secondary antibodies conjugated to horseradish peroxidase (HRP) (Southern biotech) were used at a 1:5000 dilution and a chemiluminescent signal was generated using the Pierce ECL Western Blotting Substrate (Thermo Fisher) ...
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bioRxiv - Immunology 2024Quote: ... goat anti-mouse IgG antibody (Southern Biotech, Birmingham, AL). Plates were washed four times ...
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bioRxiv - Cancer Biology 2024Quote: ... an anti-human IgG1 secondary antibody (SouthernBiotech, #9054-09) was employed to detect Dara-and Isa-bound cells ...
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bioRxiv - Cell Biology 2024Quote: ... and anti-rabbit IgG antibody (4030-05; Southern Biotech).
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bioRxiv - Immunology 2021Quote: ... Plates were washed 5 times with PBS/0.05% Tween20 and IgG antibodies were detected using horseradish peroxidase (HRP)-conjugated goat anti-human IgG detection antibody (Southern Biotech, Birmingham, AL, USA) at a 1:4,000 dilution and incubated for 90 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... incubated with primary antibody (starting concentration 10 µg/ml) in PBS for 120 min and secondary antibody (anti-human IgG-HRP; Southern Biotech 2040-05) diluted 1:2500 in 1% BSA in PBS for 60 min at RT ...
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bioRxiv - Immunology 2024Quote: ... Antibody secreting cells were detected with alkaline phosphatase (AP)-conjugated antibodies goat anti-mouse IgM (0.5 µg/mL; SouthernBiotech Cat#1020-04, RRID:AB_2794200) or goat anti-mouse IgG (0.5 µg/mL ...
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bioRxiv - Microbiology 2020Quote: ... IgG-bound DNP-beads were next incubated with 1 µg/mL Alexa Fluor647-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech) in PBS-TH.
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bioRxiv - Immunology 2021Quote: ... were coated with 2.5 µg/ml of mouse anti-human c-Myc 9E10 mAb (produced in house) or goat anti-human IgG (1:2000, Southern Biotech) for the standard ...
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bioRxiv - Biochemistry 2020Quote: ... and then incubation for 30 mins at room temperature with anti-human IgG HRP at 1:4000 (Southern Biotech #2040-05). 5 washes of TBST were done followed by development using 1-Step Ultra TMB-ELISA Substrate Solution (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were washed in 1 x PBS three times and mounted and then cover-slipped with Fluoromount-G with DAPI (Southern Biotech).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... before adding 50 μL/well of a 1:5000 dilution of HRP-conjugated goat anti-mouse IgG (Southern Biotech, #1030-05) in TBSF+MnCl2 ...
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bioRxiv - Microbiology 2022Quote: ... the beads were incubated with 1 µg/mL Alexa Fluor647-conjugated goat F(ab’)₂ anti-human kappa (Southern Biotech, 2062-31) in PBS-TH for 30 min at 4 °C with shaking ...
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bioRxiv - Immunology 2022Quote: ... a standard curve for mouse isotype was established for each plate by coating 2 columns with 50 μL goat anti-mouse Ig (1 μg/mL in PBS; Southern Biotech) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... 3 columns on each plate were coated with 1:1000 dilution each of goat anti-mouse Kappa and Lambda light chains (Southern Biotech). After overnight incubation at 4ºC ...
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bioRxiv - Microbiology 2020Quote: ... in PBST or PBSTF or 1:1000 anti-hamster-IgG2/IgG3-HRP in PBST or PBSTF (Southern Biotech Cat. #1935-05). Plates were incubated at room temperature for 1-2 h ...