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Citations for Millipore Sigma :
251 - 300 of 2403 citations for rno mir 155 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Ant-miR-218 or Ant-scrambled was dissolved in sterile PBS (Sigma-Aldrich, Oakville, ON, Canada) at a final concentration of 0.3nM and infused with a total volume of 0.5μl of Ant-miR-218 or Ant-scrambled over a 7-min period as in(31 ...
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bioRxiv - Neuroscience 2019Quote: ... Ant-miR-218 or Ant-scrambled were dissolved in sterile PBS (Sigma-Aldrich, Oakville, ON, Canada) at a final concentration of 0.3 mM ...
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bioRxiv - Cancer Biology 2020Quote: ... Genomic regions containing guide sequences are PCR amplified using paired primers with 25 uL 2x JumpStart Taq Polymerase Ready Mix (Sigma-Aldrich), 1.5 uL 10 uM primer pair (CropSeq_NGS_P7 ...
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bioRxiv - Microbiology 2022Quote: ... a first PCR was performed using the templates and primers shown in Table 1 with KOD hot start DNA polymerase (Sigma-Aldrich), with annealing time of 30 seconds at the indicated temperature ...
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bioRxiv - Plant Biology 2022Quote: Conserved regions were amplified in a standard Polymerase Chain Reaction (PCR) using genome-specific primers (Linares et al., 1998; Liu et al., 2019) synthesized commercially (Sigma-Aldrich). These probes were labelled with digoxigenin-11-dUTP ...
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bioRxiv - Microbiology 2023Quote: ... The cassette corresponding the DHFR-pSAG1-TgAPT1-GFP cassette was amplified by PCR from the plasmid of the same name using primers ML3088/ML3089 with the KOD DNA polymerase (Sigma-Aldrich). This donor sequence was then used to transfected to Pru background ...
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bioRxiv - Genetics 2023Quote: ... Our method of genotyping involved PCR amplification across mutation sites using the primers described in Table 1 (purchased from Sigma Aldrich). Then ...
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bioRxiv - Biochemistry 2024Quote: ... R-domain phosphorylation sites and interacting residues mutants were generated by site-directed mutagenesis using a Q5-New England Biolabs PCR mutagenesis kit together with primers synthesized from Millipore sigma and verified by sequencing (Elim Biopharmaceuticals ...
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bioRxiv - Plant Biology 2019Quote: ... The allele-specific forward primers and common reverse primers were synthesised by Sigma-Aldrich. Allele-specific primers were synthesised with standard FAM or VIC compatible tails at their 5’ ends (FAM tail ...
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bioRxiv - Microbiology 2021Quote: ... Top2β: Forward primer 5’CAGCCCGAAAGACCTAAATAC and reverse primer 5’ATCTAACCCATCTGAAGGAAC obtained from Sigma-Aldrich.
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bioRxiv - Plant Biology 2023Quote: ... 8 pmol M13-labeled forward primer and 32 pmol reverse primer (Sigma Life Science), standard Taq buffer (New England Biolabs) ...
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bioRxiv - Developmental Biology 2024Quote: ... Primers were designed using Primer-BLAST (National Institutes of Health) and obtained from Sigma. For each primer pair ...
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bioRxiv - Cell Biology 2020Quote: ... Antibodies were diluted in TBSN (10 mM Tris-Cl pH 7.4, 155 mM NaCl, 1% IGEPAL CA-630 (Sigma-Aldrich #I8896), 1% BSA ...
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bioRxiv - Biochemistry 2024Quote: ... The following antibodies were used in the experiment: phospho(p)-IR tyr972 (1/800, 155 kDa; 07-838, Sigma-Aldrich, USA), p-IRS1 r307 (1/800 ...
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bioRxiv - Microbiology 2021Quote: ... and oligonucleotide primers (Sigma-Aldrich) used for experimental work in this study are listed in Table 1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Primers were synthesized by Sigma and IDT.
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bioRxiv - Neuroscience 2019Quote: ... The following primers (Sigma-Aldrich), mapping outside the ssODN sequence were used to amplify a region of ≈ 700 bps containing the edited region of the Pde6b gene ...
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bioRxiv - Microbiology 2019Quote: ... Primers were purchased from Sigma and their sequences listed in Table 3 ...
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bioRxiv - Neuroscience 2020Quote: ... Primers used (Sigma, Dublin, Ireland) are listed in Table S4.
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bioRxiv - Neuroscience 2021Quote: ... The DNA primers (Sigma-Aldrich) used for genotyping are listed in Table S7.
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bioRxiv - Bioengineering 2020Quote: ... Custom designed primers (Sigma-Aldrich) with the sequences presented in Table 1 were used as myogenic and immunogenic markers ...
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bioRxiv - Neuroscience 2020Quote: ... Primers were ordered from Sigma as lyophilized and reconstituted in Milli-Q water to a stock concentration of 100 μM and further diluted to a 10 μM working stock ...
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bioRxiv - Physiology 2022Quote: ... with Random Primers (Sigma-Aldrich) and incubated for 3 min at 70°C followed by addition of dNTPs ...
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bioRxiv - Neuroscience 2022Quote: ... Forward and reverse primers (Sigma) targeting the telomere region and the 36B4 gene (single copy gene ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... All primer probes (Sigma-Aldrich) were designed to span exon boundaries ensuring amplification of only mRNA ...
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bioRxiv - Cell Biology 2019Quote: ... Primers were synthetized by Sigma. qPCR reactions were performed on a LightCycler® 480 Real Time PCR System (Roche ...
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bioRxiv - Cancer Biology 2019Quote: ... Primers were designed (Sigma Aldrich) and listed in Supplementary Table S3 ...
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bioRxiv - Physiology 2020Quote: ... with Random Primers (Sigma-Aldrich) and incubated for 3 min at 70°C followed by addition of dNTPs ...
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bioRxiv - Microbiology 2021Quote: ... plasmids and primers (Sigma, Singapore) used in this study are listed in S6 and S7 Tables ...
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bioRxiv - Microbiology 2021Quote: ... and desalted primers (Sigma-Aldrich). The primers used in this study are shown in Table S4 ...
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bioRxiv - Molecular Biology 2020Quote: ... Sequences of primers (Genomed/Sigma) and annealing temperatures are listed in Table S2 in Supplementary Material ...
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bioRxiv - Cell Biology 2022Quote: Primers were purchased from Sigma/Merck or IDT ...
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bioRxiv - Microbiology 2023Quote: ... The primers (Sigma-Aldrich, Bangalore) for q-PCR are listed in Table S4 ...
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bioRxiv - Neuroscience 2022Quote: KiCqStart SYBR green primers (Sigma) were used for quantitating the mRNA levels (Table-2 shows the primers used for quantitating miRNAs and mRNAs in ...
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bioRxiv - Molecular Biology 2022Quote: ... The primer sequences (Genomed/Sigma) and annealing temperatures are listed in Table S1 in the Supplementary Material.
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bioRxiv - Neuroscience 2022Quote: ... specific oligonucleotide primers (Sigma Aldrich) of the target genes and SYBR Green (KAPA Syber1 FAST PCR Master Mix (2X ...
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bioRxiv - Microbiology 2023Quote: ... The primers (Sigma-Aldrich, Bangalore) for q-PCR are listed in supplementary tables S4 and S5 ...
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bioRxiv - Physiology 2023Quote: ... with Random Primers (Sigma-Aldrich) and incubated for 3 min at 70°C followed by addition of dNTPs ...
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bioRxiv - Developmental Biology 2023Quote: ... forward and reverse primers (Sigma), and nuclease free water to determine mRNA expression of MMPs 2,9 ...
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bioRxiv - Developmental Biology 2023Quote: ... forward and reverse primers (Sigma), and nuclease free water to determine mRNA expression of MMPs 2,9 ...
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bioRxiv - Biochemistry 2023Quote: ... Primers were obtained from Sigma. For primer sequences ...
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bioRxiv - Physiology 2024Quote: ... Commercially available KiCqStart primers (Sigma) were used to measure mRNA expression of guinea pig Igf2 ...
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bioRxiv - Molecular Biology 2023Quote: FLAG-tagged or HA-tagged cDNA fragment of DDX43 was amplified by RT-PCR from total RNA of silkworm ovaries and cloned into pIEx-1 vector (Merck Millipore/Novagen) or pIExZ vector by In-fusion cloning kit (Takara).
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bioRxiv - Cancer Biology 2022Quote: ... Lysis buffer supplemented with freshly prepared protease inhibitors (Protease Inhibitor Cocktail Set I #539131 and Protease Inhibitor Cocktail Set III #539134, Millipore Sigma-Aldrich) and phosphatase inhibitors (Phosphatase Inhibitor Cocktail Set I #524624 and Phosphatase Inhibitor Cocktail Set II #524625 ...
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bioRxiv - Cell Biology 2020Quote: ... and RT-qPCR was performed as described previously [48] using QuantiTect SYBR Green PCR kit and KiCqStart SYBR Green optimized primers from Sigma-Aldrich (KSPQ12012).
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bioRxiv - Cell Biology 2021Quote: ... Primers were designed using the NCBI Primer designing tool and custom-synthesized by Sigma-Aldrich. The sequences of primers were listed in Supplementary Table ...
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bioRxiv - Developmental Biology 2022Quote: ... with 200 nM of forward and reverse primers (Sigma-Aldrich; primers listed in table 2) on a QuantStudio 5 (Applied Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: ... The primers were predesigned and synthesized by Sigma-Aldrich (KiCqStart® SYBR® Green Primers). 20 ng cDNA was added to each well of a 96-well PCR plate with 1 μM forward and reverse primer for each nucleator ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 µl protease inhibitor cocktail set (EDM Millipore 539134), and 10 µl murine RNase inhibitor (200U ...
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bioRxiv - Neuroscience 2021Quote: ... and sterilized using stericup and steritop filtration set (Millipore). Dentate gyrus and SVZ were microdissected using SteREO Discovery V8 microscope (Zeiss ...