Labshake search
Citations for Millipore Sigma :
101 - 150 of 2449 citations for hsa mir 320a RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... 1 g/L D-glucose with 1.25% human serum albumin (HSA) (Sigma) and either physiologic (0.1 nM ...
-
bioRxiv - Biochemistry 2023Quote: Ligand binding to commercial wild type HSA (non-defatted, Sigma-Aldrich A1653) and to HSA1 variant (His-tagged ...
-
bioRxiv - Plant Biology 2020Quote: ... The PCR conditions were set up to amplify only the short 670 bp product using REDExtract-N-Amp PCR ReadyMix (Sigma). PCR conditions ...
-
bioRxiv - Genomics 2019Quote: ... was used along with 0.36 μl of 50 μM custom-designed SYNE2 short and long isoform primer and 0.5 μl of 10 μM probe set (Table 1, obtained from Sigma-Aldrich) and 1 μl of the primer limited cyclophilin A (PPIA ...
-
bioRxiv - Immunology 2022Quote: ... qPCR was performed using PerfeCTa SYBR Green FastMix Reaction Mix (Green Fastmix, ROX™) (QuantaBio) and KiCqStart primer sets (Sigma). ELISAs were performed as per the manufacturer’s instructions (R&D Systems).
-
bioRxiv - Neuroscience 2021Quote: ... Overlapping PCR primers encoding the gRNA sequences were ordered from Sigma-Aldrich and Eurofins Genomics ...
-
bioRxiv - Genomics 2022Quote: ... PCR amplifications were set up in 25μl total volume reaction mixture containing water (Sigma-Aldrich) (18.4μl) ...
-
bioRxiv - Biochemistry 2020Quote: Fatty acid and globulin-free HSA was purchased from Sigma-Aldrich (Product # A3782). HSA was dissolved in a buffer containing 50 mM potassium phosphate (pH 7.0 ...
-
bioRxiv - Immunology 2022Quote: ... and stimulated with 50 ng/ml DNP-HSA (human serum albumin)(A6661, Sigma). For DN2 and DN3 cell culture ...
-
bioRxiv - Neuroscience 2023Quote: ... we were blocking the sections in 1 % human serum albumin (HSA; Sigma-Aldrich) in TBS for 1 h ...
-
bioRxiv - Immunology 2024Quote: ... for 30 min followed by stimulation with 0.2 μg/ml HSA-DNP (Sigma) for 30 min ...
-
bioRxiv - Cancer Biology 2022Quote: ... RT-PCR products were separated by gel electrophoresis in 1% agarose (Sigma). Gene-Ruler 100 bp DNA ladder (ThermoScientific ...
-
bioRxiv - Genetics 2021Quote: ... The induction of the top 10 HITS from DUSP16 and KSR1 were validated in these samples via qPCR (PCR primer sequences used are shown in Additional file 1: Table S3 primers were obtained from Millipore Sigma unless mentioned otherwise from Integrated DNA Technologies) ...
-
bioRxiv - Genetics 2021Quote: ... The induction of the top 10 HITS from IFIH1 were validated in these samples via qPCR (PCR primer sequences used are shown in Additional file 1: Table S3; primers were obtained from Millipore Sigma unless mentioned otherwise from Integrated DNA Technologies) ...
-
bioRxiv - Microbiology 2021Quote: Colonies that produced bands of the expected size with both primer sets were inoculated into 1 ml of LB containing 1% arabinose (Sigma-Aldrich) for 1 hour at 30°C to induce I-Sce expression to linearize the inserted KanSacB cassette ...
-
bioRxiv - Immunology 2022Quote: ... and the heavy and light chain sequences of the murine hybridomas were by obtained using the mouse Ig-primer set (69831; EMD Millipore) as described (83) ...
-
bioRxiv - Cancer Biology 2023Quote: miR-6883 mimic was purchased from Sigma-Aldrich (HMI2616). This double-stranded RNA molecule mimics endogenous mature miRNA when introduced into cells.
-
bioRxiv - Synthetic Biology 2021Quote: ... DNA fragments were either PCR-amplified with oligonucleotide primers (Sigma-Aldrich and IDT) or ordered as gBlocks from IDT and cloned into Gateway pENTR plasmids ...
-
Liver X Receptor activation regulates genes involved in lipid homeostasis in developing chondrocytesbioRxiv - Physiology 2019Quote: ... and all real-time PCR reagents and primers were obtained from Sigma-Aldrich. All organ culture and cell culture media components were obtained from Sigma-Aldrich and Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2019Quote: ... with PCR primers with overhangs containing restriction sites for SpeI and NotI (Sigma). pLVX-EF1α-IRES-ZsGreen1 (Cat# 631982 ...
-
bioRxiv - Immunology 2023Quote: ... and transcripts quantified by real-time semi-quantitative PCR using KiCqStart primers (Sigma) and iTaq SYBR green (Accuscience ...
-
bioRxiv - Microbiology 2023Quote: ... PCR assay was conducted by using gene specific primers procured from Sigma-Aldrich. The gene PA3617 (recA ...
-
bioRxiv - Neuroscience 2024Quote: ... Primers for SYBR Green-based PCR of mRNA were obtained from Sigma Aldrich, miRCURY LNA assays for qPCR of miRNA were purchased from Qiagen ...
-
bioRxiv - Biochemistry 2019Quote: Accessible amino groups in HSA were detected using fluorescamine (Sigma-Aldrich, St. Louis, USA) as described 54 ...
-
bioRxiv - Biophysics 2022Quote: ... De-fatted Human Serum Albumin (DE-HSA) lyophilized powder was purchased from Sigma-Aldrich (product no ...
-
bioRxiv - Developmental Biology 2023Quote: ... The digestion was terminated by α-MEM media with 10% HSA (Merck Millipore, 823022). After digestion ...
-
bioRxiv - Immunology 2023Quote: ... cells were stimulated as indicated with different concentrations of Ag (DNP-HSA, Sigma, #A6661). The degree of degranulation was determined by quantification of β-hexosaminidase release as described previously (40).
-
bioRxiv - Developmental Biology 2021Quote: ... qRT-PCR was performed with SYBR Green Quantitative RT-qPCR Kit (QR0100; Sigma) or Kicqstart One-Step Probe RT-qPCR ReadyMix (KCQS07 ...
-
bioRxiv - Cell Biology 2022Quote: Individual cells were sorted into 96-well plates containing 4μL of lysis buffer and index RT primers (0,2% Triton (Sigma Aldrich), 0,4 U/μL RNaseOUT (Thermofisher Scientific) ...
-
bioRxiv - Neuroscience 2022Quote: Genotyping for the R1.40 transgene locus was done with the recommended PCR primers using the PCR Ready Mix kit (E3004; Sigma-Aldrich). The sequences of the genotyping primers were as follows ...
-
bioRxiv - Biochemistry 2022Quote: ... The sequence was verified by colony PCR using standard primers (Sigma-Aldrich, Taufkirchen, Germany) and Taq polymerase (In house ...
-
bioRxiv - Genetics 2023Quote: ... specific primers were designed and PCR was performed using KOD DNA polymerase (Sigma Aldrich). The amplification of DNA fragments was done following manufacturer’s instructions into a Bioer GeneExplorer thermal Cycler ...
-
bioRxiv - Cell Biology 2023Quote: ... cDNAs were mixed with gene specific primers and SYBR green PCR MasterMix (Sigma Aldrich) and run on an Applied Biosystems 7900HT Fast Real-Time PCR system.
-
bioRxiv - Microbiology 2024Quote: ... amplified with the primers P2 and P3 (S2 Table) by KOD PCR (Sigma-Aldrich). Transfected parasites were selected in medium containing mycophenolic acid (25 μg/ml ...
-
bioRxiv - Cancer Biology 2019Quote: ... miR-325 and control miRNA were purchased from Sigma-Aldrich.
-
bioRxiv - Bioengineering 2021Quote: ... Fluorescein isothiocynate human serum albumin (FITC-HSA) was purchased from Sigma Aldrich (Oakville, ON, Canada). Milrinone was purchased from Selleck Chemicals (Burlington ...
-
bioRxiv - Immunology 2019Quote: ... Coverslips were washed with PBS and coated with 3% human serum albumin (HSA; A1653, Sigma) o.n ...
-
bioRxiv - Molecular Biology 2019Quote: ... Primers are from Sigma (KiCqStartTM Primers).
-
bioRxiv - Plant Biology 2022Quote: All primers (Sigma Aldrich UK. Primers) for gene targets (Supplementary Table S1) ...
-
bioRxiv - Cell Biology 2021Quote: ... qRT-PCR was performed with Kicqstart One-Step Probe RT-qPCR ReadyMix (KCQS07; Sigma). Inventoried TaqMan probes for qRT-PCR from Applied Biosystems were used for human TULP3 and GAPDH ...
-
bioRxiv - Plant Biology 2022Quote: ... Quantitative RT-PCR was carried out using SYBR Green (Jumpstart Taq ReadyMix: Sigma-Aldrich) in 10 μl volumes for 3 biological and 3 technical replicates with 1:10 diluted cDNA ...
-
bioRxiv - Cell Biology 2023Quote: ... Quantitative RT-PCR was performed using FastStart Universal SYBR Green Master (Rox) (Sigma, 4913850001) on a Bio-Rad CFX Connect Real-Time PCR detection system ...
-
bioRxiv - Immunology 2023Quote: ... IL10 and normalization control GAPDH were examined by RT-qPCR using gene specific primers (Sigma Aldrich, St. Louis, MO, USA) as previously described 11 ...
-
bioRxiv - Biochemistry 2021Quote: ... the Msm guaB1 gene was amplified by PCR by primer pair 17/18 and inserted into the PCR-linearized pTriex-4 vector (Novagen, USA) with primers 15/16 by the In-Fusion approach ...
-
bioRxiv - Genomics 2020Quote: ... Sequences were PCR amplified from zebrafish genomic DNA using the primers in SupplementaryTable16 (Sigma-Aldrich), with the proof-reading iMax ™ II DNA polymerase (INtRON Biotechnology ...
-
bioRxiv - Molecular Biology 2019Quote: ... using PCR primers Ad1_noMX and Ad2_Barcode (Sigma-Aldrich, see Table 2, (Buenrostro et al., 2013) for 5 initial cycles (1 cycle ...
-
bioRxiv - Immunology 2022Quote: ... cDNAs were mixed with indicated gene-specific primers and SYBR green PCR Master Mix (Sigma), and qRT-PCR was performed on an Applied Biosystems 7900HT Fast Real-Time PCR system.
-
bioRxiv - Physiology 2023Quote: ... and included predesigned primers (Sigma KiQStart Primers). Gene expression was normalized using housekeeping genes β-ACTIN ...
-
bioRxiv - Neuroscience 2021Quote: ... This was followed by blocking for 1 hour in 1% human serum albumin (HSA; Sigma-Aldrich) and 0.03-0.1% Triton X-100 dissolved in TBS and 200 ug/ml Digitonin (D141-100MG ...
-
bioRxiv - Physiology 2019Quote: ... human tubal fluid (HTF) medium and human serum albumin (HSA) (Merck Millipore Corporation, Billerica, MA, USA), Fluo-4 AM and Pluronic F-127 (Molecular Probes ...