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Citations for Millipore Sigma :
1 - 50 of 3692 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... hsa-miR22-3p (Sigma #HSTUD0393) or 100 nM of hsa-let7b-5p miRNA mimic (Sigma #HMI0007 ...
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bioRxiv - Genetics 2022Quote: ... or miR-27b-3p mimics (Sigma) for 48 h ...
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bioRxiv - Cancer Biology 2021Quote: ... Standard RT-PCR using independent sets of Predesigned KiCqStart® SYBR® Green Primers (Sigma, Table S11) was carried out for validation with independent biological replicates from 2D cultures (n = 2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Hsa-miR-203 mimics were purchased from Sigma Aldrich (MISSION microRNA mimics) and transient transfection was performed using Lipofectamine 2000 (Sigma) ...
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bioRxiv - Physiology 2020Quote: ... The mouse RT-PCR primers (Sigma-Aldrich) used are shown in Supplementary Tab ...
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bioRxiv - Microbiology 2023Quote: ... RT-PCR was performed utilizing the S-GAG set of primers and probe (Cline et al., 2005)(Sigma-Aldrich, Burlington, MA) with a 1-step Master mix (ThermoFisher ...
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bioRxiv - Immunology 2022Quote: ... Primer of miR-31 was ordered from Sigma (MIRAP00090) and RNU6-1 was used as the internal control (Sigma ...
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bioRxiv - Bioengineering 2020Quote: ... The nucleotide sequences for RT-PCR and real-time PCR primers (synthesized by Sigma-Aldrich) are shown in Table 1 ...
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bioRxiv - Neuroscience 2020Quote: ... HSA (Sigma), mouse transferrin receptor 2 ectodomain (TfR2-ECD ...
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bioRxiv - Bioengineering 2020Quote: HSA (Sigma) was directly labeled with AF647 ...
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bioRxiv - Immunology 2020Quote: ... Primers for Rnu6 and miR-21 were purchased from Sigma Aldrich. Primers for β-actin ...
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bioRxiv - Cancer Biology 2024Quote: ... Primer sets were purchased from Sigma-Aldrich’s KiCqStartTM line.
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bioRxiv - Cell Biology 2022Quote: ... 400nM RT primers (Sigma Aldrich)) and scRNA-seq primers ...
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bioRxiv - Bioengineering 2019Quote: ... Primer probe sets were custom oligos (Sigma-Adrich) (Table 4).
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bioRxiv - Bioengineering 2022Quote: ... HSA (Sigma, #A4327) was added to the serum-free growth media at the desired concentration ...
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bioRxiv - Genetics 2020Quote: ... HSA (Sigma A8763), Hemoglobin (Sigma H2500) ...
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bioRxiv - Immunology 2023Quote: HSA (Sigma Aldrich) was incubated in 50 mM Tris buffer (pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... The 3’UTR was determined by RT-PCR with an oligo-dT anchor primer (Sigma) as described58 with primers found in Table 4 ...
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bioRxiv - Microbiology 2020Quote: ... RT and PCR primers for the determination of the 3’UTR were obtained from Sigma. The remaining primers were purchased from TAG Copenhagen.
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bioRxiv - Cell Biology 2020Quote: ... miRNA-147-3p or miR-298-5p mimics (Dharmacon) and incubated for 72 hours in DMEM/OptiMEM medium (Sigma-Aldrich/Thermo Fisher Scientific) prior lysis.
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bioRxiv - Immunology 2022Quote: ... Real time RT-qPCR was performed using PerfeCTa SYBR Green FastMix Reaction Mix (Green Fastmix, ROX™) (QuantaBio) and KiCqStart primer sets (Sigma). For tissue conditioned media (TCM ...
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bioRxiv - Immunology 2022Quote: ... Real time RT-qPCR was performed using PerfeCTa SYBR Green FastMix Reaction Mix (Green Fastmix, ROX™) (QuantaBio) and KiCqStart primer sets (Sigma).
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bioRxiv - Systems Biology 2019Quote: A mouse IgG primer set from Novagen (#69831-3) was used to amplify the IgG VH and VL regions from selected hybridoma clones ...
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bioRxiv - Bioengineering 2020Quote: ... All primer sets have been synthesized by Sigma-Aldrich DNA oligos design tool ...
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bioRxiv - Biochemistry 2023Quote: HSA (Sigma-Aldrich Canada) was immobilized on UltraLink Biosupport (ThermoScientific ...
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bioRxiv - Microbiology 2024Quote: ... with commercial RT-qPCR primers (KiCqStart SYBR Green Primers, Millipore Sigma KSPQ12012) and was assayed in Applied Biosystems QuantStudio 3 Real Time PCR System with standard cycling conditions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... All PCR primers (Sigma) were reconstituted in molecular grade water (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... All PCR primers (Sigma) are provided in Supplementary Table 1.
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bioRxiv - Cell Biology 2023Quote: ... and primer sets ordered from Sigma-Aldrich (Supplementary Table 2). Each master mix contained a 1X concentration of TB Green® Premix Ex Taq II (TLi RNaseH Plus) ...
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bioRxiv - Physiology 2019Quote: ... for synthesizing the probes was amplified using the primers described in Table S3 as following: the PCR reaction was set with KOD hot start master mix (#71842, Novagen), according to the manufacturer’s instructions and subsequently purified (#28706 ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% HSA (Merck Millipore, 823022), 1% penicillin/streptomycin (Gibco ...
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bioRxiv - Synthetic Biology 2023Quote: ... which can be PCR amplified (Phanta Max Super-Fidelity DNA Polymerase, Vazyme) with a set of primers (ordered from Microsynth or Sigma-Aldrich) to add a unique variable linker ...
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bioRxiv - Neuroscience 2020Quote: ... and human serum albumin (HSA; Sigma) and incubated at 4°C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... Templates cDNA fragments for the synthesis of riboprobes were obtained by PCR using specific primer sets (Table 1) and subcloned into the plasmid pSTBlue-1 (Novagen, Madison, WI). Nucleotide sequencing revealed 100% homologies of these fragments to the reported sequences in Genbank ...
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bioRxiv - Microbiology 2020Quote: ... A set of recombinant RT standards (EMD Millipore, Molsheim, France; catalog# 382129) ranging from 5.12e3 nU/mL to 5.12e9 nU/mL was used to estimate the nU/mL of reverse transcriptase activity in the FIV stock.
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Significant control of Zika infection in macaques depends on the elapsing time after dengue exposurebioRxiv - Immunology 2019Quote: ... Real-time RT-PCR (TaqMan) assay-specific primers and probes for ZIKV were designed by Sigma-Aldrich (St Louis, MO) following the protocol developed by the Molecular Diagnostics and Research Laboratory Centers for Disease Control and Prevention (CDC) ...
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bioRxiv - Developmental Biology 2022Quote: ... A probe for Nr2f2 was prepared by amplifying a cDNA fragment by RT-PCR (primers provided below, Table 4) from total RNA isolated from E9.5 embryos with Tri Reagent (Sigma #93289) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... and universal PCR primer (Sigma-Aldrich) were used for RT-qPCR ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR primers (Sigma Aldrich, Taufkirchen, Germany) and probes (Roche Universal Probe Library ...
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bioRxiv - Immunology 2023Quote: ... and RT-PCR amplification data (Sigma). Samples were pooled and normalized to 10nM ...
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bioRxiv - Immunology 2024Quote: ... and 0.2 µg/ml HSA-DNP (Sigma), 20 nM PMA (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR primers were ordered as dried pellets from Sigma-Aldrich. The oligonucleotides were dissolved in 1X TE pH 8.0 to a final concentration of 100 μM and stored at 4 °C ...
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bioRxiv - Bioengineering 2019Quote: ... The antibodies used were mouse anti-human HSA (1:500; clone HSA-11, A6684; Sigma Aldrich; St. Louis, MO) and 10 nm colloidal gold-conjugated goat anti-mouse IgG (1:40 ...
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The conserved metalloprotease invadolysin is present in invertebrate haemolymph and vertebrate bloodbioRxiv - Cell Biology 2019Quote: ... PCR was performed using invadolysin primers (Sigma) designed to selectively amplify the invadolysin transgene ...
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bioRxiv - Cell Biology 2021Quote: ... PCR primers were purchased from Sigma-Aldrich. Zebrafish primer sequences have been previously published (Lim et al. ...
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bioRxiv - Microbiology 2019Quote: ... PCR primers were purchased from Sigma-Aldrich and are listed in S7 Table ...
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bioRxiv - Genomics 2021Quote: ... PCR primers were obtained from Sigma-Aldrich at 100 µM concentration in H2O ...
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bioRxiv - Immunology 2023Quote: ... and the following PCR primer sequences (Sigma): Gapdh (F ...
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bioRxiv - Cancer Biology 2024Quote: ... The PCR primers were purchased from Sigma, and the Biotin-TEG DNA antisense oligos were designed in Stellaris probe designer and purchased from IDT ...
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bioRxiv - Cancer Biology 2022Quote: ... An aliquot (20 - 50 ng) of cDNA and a Forward/Reverse primer set (Sigma) were used in each PCR reaction ...