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Citations for Millipore Sigma :
101 - 150 of 10000+ citations for Zika Virus VLP E prM M Proteins since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... E-64 (Sigma-Aldrich; #324890), doxycycline (Sigma-Aldrich ...
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bioRxiv - Genomics 2020Quote: ... and 4.5 × 10−4 M monothioglycerol (Sigma M-6145). After 48 hr in culture ...
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bioRxiv - Microbiology 2020Quote: ... VLP samples were separated by 10% or 14% SDS-PAGE later blotted to PVDF membrane (Immobilon®-FL, Merck Millipore) and blocked overnight with ODYSSEY® blocking buffer ...
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bioRxiv - Microbiology 2024Quote: ... The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012, Sigma, 1:1,000). Loading control for cell lysate was detected with anti-α-tubulin (rabbit polyclonal ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were extracted either in 100 μl 3 M guanidinium hy-drochloride (Gnd-HCl, Sigma-Aldrich, G3272) in 0.1 M Tris ...
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bioRxiv - Molecular Biology 2020Quote: ... 3 mg total protein were mixed with 2 mL Urea Buffer (UB) (8 M urea (Sigma, U5128) in 0.1 M Tris/HCl pH 8.0 and 50mM ammonium bicarbonate) ...
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bioRxiv - Cancer Biology 2023Quote: ... 30 μL of protein extract was mixed with 200 μL of UA (8 M urea (Sigma, U5128) in 0.1 M Tris/HCl pH 8.5 ...
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bioRxiv - Molecular Biology 2021Quote: Retroviral infection was conducted in 6-well plates using either 1 mL of unconcentrated virus or 200 μL of concentrated virus in 20 mM HEPES pH 7.4 and 8 μg/mL polybrene (Sigma-Aldrich). If selection was desired ...
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bioRxiv - Cancer Biology 2020Quote: ... Virus infection was carried out by incubating cells with cell medium containing indicated virus and 5 μg/ml polybrene (Sigma) for 8-12 hours ...
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bioRxiv - Synthetic Biology 2023Quote: ... 6 mL of 25% m/m potassium hydroxide (Sigma, USA) in methanol (Sigma ...
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bioRxiv - Biochemistry 2022Quote: Purified full-length E-cadherin was concentrated to 8-10 μM and mixed with the nanodisc scaffolding protein MSP1D1 (Sigma) at a 5 fold molar excess ...
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bioRxiv - Molecular Biology 2022Quote: Recombinant proteins used for biochemical assays were expressed from pMal-RT plasmids (see above and Table S3. For each protein preparation, E. coli Rossetta2 CapR cells (Novagen) containing freshly transformed expression constructs were plated on LB agar containing ampicillin (100 µg/mL ...
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bioRxiv - Neuroscience 2020Quote: Virus was produced by polyethylenimine (PEI; Sigma-Aldrich) transfection of 89-90% confluent HEK293T cells with the viral vector together with the lentiviral packaging plasmids ...
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... or an empty vector control virus (Sigma SHC001V) followed by puromycin (1 μg/mL ...
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bioRxiv - Molecular Biology 2020Quote: ... CK-M (Sigma), α-actinin (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... 0.13 M citric acid [Golden Bell], 0.08 M sodium citrate [Golden Bell] buffer [pH 4], 0.15 M hydroquinone [Sigma-Aldrich], 0.005 M silver nitrate [JT Baker]) for 90 min in the dark at room temperature ...
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bioRxiv - Immunology 2020Quote: ... all CIS43 VLPs and wild-type (WT) Qβ VLPs were depleted of endotoxin by three rounds of phase separation using Triton X-114 (Sigma-Aldrich), as described in reference 48 ...
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bioRxiv - Immunology 2022Quote: ... or MDM (106) were treated for 1h at 37°C with 1 ml of VLPs-VPX+ containing 5 μg/ml polybrene (Sigma, #H9268) for SAMHD1 degradation16,37 before the addition of 20 µg CAp24 of each control or/and encoding-gene lentiviral vectors with 5 μg/ml polybrene for 2h transduction ...
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bioRxiv - Synthetic Biology 2019Quote: ... plates were coated with indicated concentrations of VLP (4°C, over-night) and subsequently blocked with 1% casein in PBS (Sigma-Aldrich) for 2h at 37°C before primary antibodies were added (polyclonal mouse anti-Pfs230 or monoclonal 1B3 antibody ...
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bioRxiv - Biophysics 2019Quote: ... Purified PCV2 VLPs were concentrated and buffer exchanged to phosphate buffered saline (PBS) using Amicon Utra-4 centrifugal filter devices (Merck Millipore, MA). PCV2 VLP samples were stored in 50-100 µl aliquots at −80°C.
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bioRxiv - Molecular Biology 2023Quote: ... ChAC-VLPs was further concentrated to 5 times of its initial volume using 100 kDa cut-off concentrator (Merck, Millipore, USA) followed by analysis on 10% Tris-Glycine SDS-PAGE and successful RBD-E2 fusion protein is validated by western blot using anti-RBD antibody.
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bioRxiv - Microbiology 2023Quote: ... The VLPs were subsequently concentrated down to 180 µL using Amicon Ultra-15 100K Centrifugal Filter Units (Milipore Sigma, MO, U.S.A). To remove any free contaminating nucleic acids ...
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bioRxiv - Cancer Biology 2021Quote: ... To transduce cells with virus cells were treated with both virus (MOI 0.5-1.0) and 5 μg / mL Polybrene infection reaction (EMD Millipore, TR-1003). For pLKO.1 vectors ...
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bioRxiv - Molecular Biology 2020Quote: ... pronase E 1 mg/ml (Sigma), and DNAse I 50 U/μl (Applichem ...
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bioRxiv - Cancer Biology 2019Quote: ... Cyclin E (1:1000, Sigma Aldrich), V5 (1/500 ...
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bioRxiv - Cell Biology 2019Quote: ... rabbit anti-CENP-E (C7488, Sigma), anti-H2A-pT120 (61195 ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM EGTA (Sigma E-4378), 1 mM MgCl2 (Mallinckrodt H590) ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM EGTA (Sigma E-4378), 1 mM MgCl2 (Mallinckrodt H590) ...
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bioRxiv - Biochemistry 2021Quote: ... Vitamin E (α-Tocopherol, Sigma-Aldrich) was dissolved in ethanol to 50mM before use in cell culture ...
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bioRxiv - Neuroscience 2022Quote: ... 20μΜ vitamin E (a-tocopherol) (Sigma) and 200mM ascorbic acid (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... and 0.1 μM Compound E (Sigma). Cells were cultured for additional 12 to 14 days with medium change every 2 days before experimental analysis.
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bioRxiv - Systems Biology 2022Quote: ... Antioxidants: Vitamin E (Sigma-Aldrich, T3251), N- acetyl-L-cystein (NAC ...
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bioRxiv - Neuroscience 2019Quote: ... and 0.1 µM Compound E (Millipore) for the first seven days ...
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bioRxiv - Immunology 2020Quote: ... 100 µL of TMB/E (Millipore) was added to each well and the absorbance at 650 nm was read after 20 min ...
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bioRxiv - Immunology 2020Quote: ... 100 µL of TMB/E (Millipore) was added to each well and the absorbance at 650 nm was read after 20 min (BioTek 800 TS) ...
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bioRxiv - Molecular Biology 2021Quote: ... or 10 μM E-64d (Sigma) or 5 mM 3-MA (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... and 1% eosin (H&E) (Sigma). For immunostaining ...
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bioRxiv - Developmental Biology 2022Quote: ... 2-mercaptoethanol (Millipore, ES-007-E), 1x penicillin/streptomycin (Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... 20ml 0.5M EDTA (Sigma, #E-5134), ddH2O to 1L) ...
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bioRxiv - Cancer Biology 2023Quote: ... (E/Z)-BCI hydrochloride (Sigma-B4313) was added to both the medium and the matrix ...
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bioRxiv - Microbiology 2023Quote: ... or E-64 (Sigma-Aldrich, E3132) at 100 µM ...
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bioRxiv - Systems Biology 2023Quote: ... hematoxylin and eosin (H&E; Sigma) for routine histological analysis ...
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bioRxiv - Biochemistry 2023Quote: ... LPS (E. coli O111:B4, Sigma) was dissolved in PBS to make a stock solution then sonicated in a water bath for 10 min prior to addition to cell suspensions ...
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bioRxiv - Cell Biology 2023Quote: ... with William’s E medium (Sigma-Aldrich) containing 2.0 g/L glucose ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.075 mg/ml pronase E (Sigma) and one drop of antifoam 204 (Sigma) ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.075 mg/ml pronase E (Sigma) and one drop of antifoam 204 (Sigma) ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.075 mg/ml pronase E (Sigma) and one drop of antifoam 204 (Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.075 mg/mL pronase E (Sigma) and one drop of antifoam 204 (Sigma) ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.075 mg/ml pronase E (Sigma) and one drop of antifoam 204 (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: Total protein from BFP expressing iNeurons was extracted using CelLytic™ M Cell Lysis Reagent (Sigma-Aldrich, #C2978) with PhosSTOP™ (Roche ...