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Citations for Millipore Sigma :
1 - 50 of 10000+ citations for Zika Virus VLP E prM M Proteins since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... ZIKA E (Millipore Sigma, cat# MABF2046), or GAPDH (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2021Quote: ... E2 (CHIK-102 and CHIK-263) [42] or Zika Virus E as a control (ZV-2, Sigma-Aldrich) (kindly provided by Dr ...
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bioRxiv - Microbiology 2020Quote: ... Cells were incubated with mouse anti-Zika antibody 4G2 (Millipore) at 1:500 dilution for 60 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... VLPs were lysed by the addition of 100 μl of Phage Lysis Buffer (4.5 M guanidinium isothiocyanate (Sigma-Aldrich Cat#50983), 44 mM sodium citrate pH 7.0 (Sigma-Aldrich Cat#C8532) ...
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bioRxiv - Microbiology 2023Quote: ... were left untreated or treated with 18.4 μM puromycin (PRM; Sigma, Merck) for 30 secs ...
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bioRxiv - Immunology 2021Quote: The naturally packaged ssRNA in Qβ-VLPs was digested as following: Qβ(ssRNA)-VLPs were washed 3x with 20mM HEPES (Sigma-Aldrich) using Amicon ultra 0.5ml tubes 100kDa MWCO ...
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bioRxiv - Microbiology 2023Quote: ... VLP and cells were stained with SYBRGreen I (Sigma) for 10 min in the dark at 80°C and let cool down for 5 min at room temperature [46] ...
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bioRxiv - Microbiology 2021Quote: ... pneumoniae-PRM were cultured on Luria-Bertani agar (LB) (Sigma-Aldrich, St. Louis, USA) containing 100 μg mL−1 of ampicillin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... Supernatants containing VLPs were filtered (Millex HV, 0.45 μm; Millipore) and frozen at −80°C until use ...
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bioRxiv - Cancer Biology 2023Quote: ... The M-protein solutions were diluted 1:1 with 8 M Urea (Sigma Aldrich) in Tris.HCl (pH=7 ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with the DENV PrM-reactive monoclonal 2H2 primary antibody (Millipore Sigma, MAB8705) at 1µg/mL ...
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bioRxiv - Genetics 2021Quote: ... Proteins were then transfered to an Immobilon-E PVDF membrane (Millipore), which was then washed with 1× PBS containing 0.05% Tween 20 (three washes ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were transferred Immobilon-E PVDF membrane (Merck Millipore, Tullagreen, Ireland) and AGAL was detected by monoclonal anti-Galactosidase alpha antibody (# ab168341 ...
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bioRxiv - Neuroscience 2022Quote: ... Pelleted proteins were resuspended in 0.1 M NaOH (Sigma) and assayed for protein quantification using the Bradford assay (Biorad).
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bioRxiv - Systems Biology 2021Quote: ... virus protein-bound host proteins were enriched by adding 50 µl anti-HA-agarose slurry (Sigma-Aldrich, A2095) with constant agitation for 3 hours at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... M (Cat No: 1115-70-4) and E (Cat No: 989-51-5) were obtained from Sigma Chemical Co (St ...
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bioRxiv - Microbiology 2023Quote: ... The VLPs were concentrated with Polyethylene glycol 8000 (Sigma-Aldrich, Cat#P2139), overnight precipitation and purification by chloroform treatment ...
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bioRxiv - Microbiology 2019Quote: ... cDNA was generated from 1 μg of RNA using Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... Proteins were resuspended in concentrated urea buffer (8 M Urea, 2 M Thiourea, 150 mM NaCl (Sigma)) ...
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bioRxiv - Immunology 2022Quote: ... Commercially available flavivirus E protein-specific mAb 4G2 (EMD Millipore, Darmstadt, Germany), rabbit anti-ZIKV prM and C antibodies (GeneTex ...
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bioRxiv - Immunology 2023Quote: ... proteins were transferred to a hydrophilic PVDF membrane (Imobilon E, Millipore-Sigma). Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000 ...
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bioRxiv - Immunology 2023Quote: ... proteins were transferred to a hydrophilic PVDF membrane (Imobilon E, Millipore-Sigma). Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000 ...
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bioRxiv - Cell Biology 2022Quote: Proteins were reduced with 2.5 μl 0.2 M dithiothreitol (Sigma) for one hour at 57 °C at pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... E-A14 and M-A14 cells and 10µg.mL−1 of Insulin-transferrin-sodium selenite media supplement (Sigma-Aldrich) for NEU15.
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bioRxiv - Microbiology 2023Quote: ... Enriched proteins were denatured in U/T buffer (6 M urea, 2 M thiourea, 1 mM DTT (Sigma), 10 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... Cleaved E protein was concentrated using a 10 kDa MWCO concentrator (Millipore Sigma) to ~ 1 mL and further purified on a Superdex 200 10/300 Increase (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... and protein concentration was measured with BCA assay (Sigma 71285-M). Then ...
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bioRxiv - Microbiology 2020Quote: ... Duplicate cDNA preparations from each sample were generated from 1 μg of RNA using Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Duplicate cDNA preparations from each sample were generated from 1 μg of RNA using Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Sigma−Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Duplicate cDNA preparations from each sample were generated from 1 μg of RNA using Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and 0.5 M NaCl) before eluting bound proteins with buffer A containing 0.5 M Methyl-α-D mannose (Sigma). Fractions containing NA activity were pooled ...
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bioRxiv - Microbiology 2024Quote: ... The protein pellet was washed in protein wash buffer (0.3 M guanidine hydrochloride (Sigma Aldrich, USA) in 95% ethanol ...
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bioRxiv - Immunology 2022Quote: ... Virus was concentrated from media using PEG Virus Precipitation Kit (Sigma). Viral titer was determined by QuickTiter™ Lentivirus Associated HIV p24 Titer Kit (Cell Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... All lentivectors and VLPs were DNAse treated (2 h at 37°C, DNaseI, Sigma) before they were concentrated by ultracentrifugation in a Sorvall Discovery (Hitachi ...
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bioRxiv - Microbiology 2023Quote: ... We collected VLP bands and buffer exchanged using a 100-kDa Amicon filter (Sigma). VLPs were buffer exchanged into DPBS (Thermo Fisher Scientific Cat # ...
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bioRxiv - Cancer Biology 2023Quote: ... The eluted M-protein fractions were collected in Eppendorf tubes prefilled with 5 μl 2 M TRIS.HCl (pH=7, Sigma Aldrich), to neutralize the solution ...
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bioRxiv - Cancer Biology 2024Quote: ... 80 μM aprotinin, 4 mM bestatin, 1.4 mM E-64, 2 mM leupeptin and 1.5 mM pepstatin A; Sigma-Aldrich, P8340) and 50 nM calyculin A (LC Laboratories ...
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bioRxiv - Biophysics 2020Quote: Proteins were buffer exchanged into 1 M aqueous ammonium acetate (Sigma-Aldrich) using P6 Biospin columns (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Whole-cell protein lysates were prepared in CelLytic M lysis buffer (Sigma) with 1% protease/phosphatase inhibitors (Sigma ...
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bioRxiv - Microbiology 2019Quote: The genes of either E protein or E DIII protein (residues 301-403) of ZIKV (GZ01, KU820898) without tag were cloned into pET28a vectors (Novagen) and expressed by IPTG-induction in BL21 (RIL ...
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bioRxiv - Developmental Biology 2021Quote: ... Total proteins were extracted from cells with the M-PER Mammalian Protein Extraction Reagent (Sigma, GE28-9412-79) supplemented with protease and phosphatase inhibitors (Roche) ...
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bioRxiv - Developmental Biology 2024Quote: ... the broad-spectrum hydroxamate metalloproteinases inhibitor CT1746 (100 µM) and the protease inhibitor cocktail (a mixture of aprotinin, bestatin, E-64, leupeptin and pepstatin A, 1/1000)(SIGMA, P1860) with or without 20 nM Wnt5a in the presence or absence of 500 nM RAP for 3 h at 37 °C ...
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bioRxiv - Cancer Biology 2020Quote: Vesicular stomatitis virus (VSV)-G protein pseudotyped lentiviruses and retroviruses were prepared by using polyethylenimine (PEI; Sigma) to co-transfect 293T cells with a lentiviral or retroviral plasmid and pantropic VSVg (Addgene ...
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bioRxiv - Microbiology 2022Quote: Coding sequences for M proteins were cloned into a modified pET28b vector (Novagen) that contained sequences encoding an N-terminal His6-tag followed by a PreScission protease cleavage site (5) ...
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bioRxiv - Neuroscience 2022Quote: ... Protein was extracted using 100 µL of lysis buffer (7 M urea (Sigma), 2 M thiourea (Sigma ...
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bioRxiv - Cell Biology 2021Quote: Protein was extracted from cells using lysis buffer (CelLytic™ M, Sigma-Aldrich) supplemented with Halt™ protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... protein was extracted from SC cells using lysis buffer (CelLyticTM M, Sigma-Aldrich) supplemented with Halt™ protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were solubilised in solubilising buffer containing 1 M hydroxylamine hydrochloride (159417, Sigma), 1 mM HPDP-biotin (A8008 ...
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bioRxiv - Microbiology 2022Quote: ... varicella-zoster virus (Light Diagnostics, Varicella-Zoster Virus DFA kit, EMD Millipore, Temecula, CA), adenovirus (D3Ultra Respiratory Virus Screening & Identification kit ...
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bioRxiv - Genetics 2023Quote: ... The blocks were cut into 4 μm sections and stained with hematoxylin and eosin (H&E) to visualize morphology and with potassium hexacyanoferrate(II) trihydrate (Sigma cat# P3289) to visualize ferric iron deposits and counterstained with Neutral Red to visualize cell morphology (Sigma-Aldrich cat#72210).