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Citations for Millipore Sigma :
151 - 200 of 8449 citations for Yellow Fever virus Envelope protein sheep Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... 5% FCS (Sigma-Aldrich), 20 μg/ml gentamycin (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... 12.5% FCS (Sigma-Aldrich), 1% PenStrep and 100IU/ml interleukin-2 (ThermoFisher) ...
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bioRxiv - Cell Biology 2023Quote: ... Fc control (Millipore, #401104), ephrinB1-Fc (R&D ...
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bioRxiv - Cancer Biology 2021Quote: ... Flag-tag (Sigma, F3165-.2MG), Veriblot IgG (Abcam ...
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bioRxiv - Genomics 2019Quote: ... Flag-tag M2 (F1804, Sigma), goat IgG control (sc-2028 ...
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bioRxiv - Microbiology 2019Quote: ... Flag-tag (Sigma-Aldrich, 1;1000) and β-actin (Sigma-Aldrich ...
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bioRxiv - Genetics 2022Quote: ... Anti-FLAG tag (Sigma, #F1365), anti-PGK1 (Thermo # PA5-28612) ...
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bioRxiv - Molecular Biology 2020Quote: ... HIS-tag (Sigma-Aldrich H1029), and Streptactin-HRP (ThermoFisher Scientific #21130) ...
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bioRxiv - Cell Biology 2021Quote: ... S tag (EMD Millipore; MAC112), IRDye 680RD Streptavidin (LI-COR ...
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bioRxiv - Cell Biology 2022Quote: ... anti-S-tag from Novagen, anti-V5 from GenScript and anti-GFP from Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2022Quote: ... HA-Tag (Sigma H3663-200UL), ACF1 (Abcam ab187670) ...
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bioRxiv - Microbiology 2023Quote: The His-tag system (Novagen) was used for Sif purification ...
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bioRxiv - Biochemistry 2023Quote: ... and FLAG tag (Sigma, F3165).
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bioRxiv - Developmental Biology 2021Quote: ... then blocked in PBT plus 5% normal sheep serum (Sigma) for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... was diluted 1:400 in 2.5% sheep serum (Sigma-Aldrich). Incubations were done for at least 36h at 4° C shaking and after each incubation time ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were subsequently blocked using 5% sheep serum (Sigma-Aldrich) (r.t. ...
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bioRxiv - Neuroscience 2021Quote: ... and sheep anti-TH (1:500, ref. # AB1542, Merck Millipore). Primary antibodies were detected after 2 hours of incubation at room temperature with appropriate secondary antibodies coupled to Alexa-Fluor 488 ...
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bioRxiv - Neuroscience 2020Quote: ... and then were blocked with 10% sheep serum (Sigma-Aldrich) in solution I containing 100 mM pH 7.5 Tris-Cl with 0.15 M NaCl at room temperature for 1 hr ...
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bioRxiv - Biochemistry 2021Quote: ... affinity purified Cul2 or IgG from sheep serum (15131, Sigma) using BS3 crosslinker (S5799 ...
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bioRxiv - Neuroscience 2021Quote: ... and sheep anti-rabbit Cy3 (Sigma–Aldrich C2306, 1:100).
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bioRxiv - Cell Biology 2023Quote: ... 5 μg/mL prolactin from sheep pituitary (Sigma; cat. #L6520), and 10 ng/mL epidermal growth factor from murine submaxillary gland (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... sheep anti-TH (Millipore Cat. No AB1542; dil. 1:1000), rabbit anti-HA (Sigma-Aldrich Cat ...
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bioRxiv - Genetics 2022Quote: ... or sheep anti-neuropeptide Y antibody (Millipore, AB1583, 1:500) at 4°C for 0.5–2.5 days ...
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bioRxiv - Molecular Biology 2021Quote: ... Bound FLAG-HA-tagged KAT6B and associated proteins were eluted with 0.3 μg/μl 3x FLAG tag peptide (Sigma) in BC100 buffer for 1 h with gentle rotation at 4°C.
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bioRxiv - Immunology 2022Quote: Antibodies against the following proteins were obtained from commercial sources and used for immunoblots: anti-flag-tag (F3165, Sigma), anti-V5-tag (R960-25 ...
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bioRxiv - Molecular Biology 2020Quote: ... the His6-tag-fusion proteins were purified from the supernatant by a HIS-Select® Nickel Affinity Gel (Sigma) column ...
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bioRxiv - Plant Biology 2019Quote: ... a C-terminal polypeptide encompassing the last 284 amino acids of the PKL protein has been expressed in fusion with a hexahistidine tag in the E.coli strain BL21DE3 Rosetta (Novagen). The protein was purified under denaturing conditions on Ni2+-nitrilotriacetic acid matrix (Ni-NTA Agarose ...
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bioRxiv - Immunology 2021Quote: ... Efficiency of the expression and purification of recombinant proteins were determined by 15% SDS-PAGE and Western blotting by using anti-his tag antibodies (1:10,000) (Sigma). Protein concentrations were quantified by comparison with a curve of BSA resolved by SDS-PAGE.
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bioRxiv - Microbiology 2023Quote: All recombinantly produced proteins were enriched via affinity chromatography using Roche cOmplete™ His-Tag Purification Resin (Millipore-Sigma) and chromatography on an AKTA Start (Cytiva) ...
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bioRxiv - Microbiology 2023Quote: All recombinantly produced proteins were enriched via affinity chromatography using Roche cOmplete™ His-Tag Purification Resin (Millipore-Sigma) and chromatography on an AKTA Start (Cytiva) ...
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bioRxiv - Biochemistry 2023Quote: ... Y730F)41 was cloned into pET28a vector incorporating a His6-tag and a SUMO tag at the amino terminus of the expressed protein and expressed in E.coli BL21 gold strain (Novagen). Cells were grown at 37°C in TB medium containing 0.1 mg/ml kanamycin and 0.050 mg/ml tetracyline to an OD600 of ∼3.0 ...
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bioRxiv - Biochemistry 2023Quote: ... the protein (with N-terminal His6 tag) was concentrated using a centrifugal filter (Amicon Ultra, MWCO 30 kDa, Sigma) to 8.11 mg/ml ...
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bioRxiv - Microbiology 2019Quote: ... Anti-influenza A virus NP antibodies (Millipore), Anti-TNK2 (A12 ...
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bioRxiv - Microbiology 2022Quote: ... Virus propagation was performed in DMEM (Sigma) supplemented with 2% fetal bovine serum (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... Virus propagation was performed in DMEM (Sigma) supplemented with 2% fetal bovine serum (Gibco) ...
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bioRxiv - Plant Biology 2023Quote: ... vertically grown 5-day old seedlings were incubated solution of Fluorol Yellow 088 (Sigma) (0.01% ...
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bioRxiv - Biochemistry 2021Quote: SUMO-peptide fusions were cloned into a pDW363 vector that appends a BAP sequence and 6x-His tag to the N-terminus of the protein and transformed into Rosetta2(DE3) cells (Novagen). Rosetta2(DE3 ...
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bioRxiv - Microbiology 2020Quote: ... The analyzed proteins contained an SHH-tag (Sarkari et al., 2014) and were detected using anti-HA (Sigma-Aldrich, USA) antibodies and anti-mouse IgG-HRP (Promega ...
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bioRxiv - Cancer Biology 2021Quote: ... The protein pellet was resuspended in digest buffer (0.1 M triethylammonium bicarbonate, pH 8.5, Sigma-Aldrich, tandem mass tag (TMT) labeling using a 6-plex TMT kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... was cloned into the protein expression vector pET28a (+) containing an IPTG-inducible promoter and a C-terminal 6x-His tag (Novagen). Expression of CosR-His was then induced in E ...
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bioRxiv - Developmental Biology 2020Quote: Coding regions for all protein fragments used for EMSA were cloned in-frame with an N-terminal 6xHis-tag in the pET14b vector (Novagen) and expressed in BL21 cells ...
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bioRxiv - Plant Biology 2024Quote: ... Phosphorylation of NopTC93S proteins was analyzed by 50μM Zn2+-Phos-tag SDS-PAGE and detected with anti–FLAG (Sigma, F1804) antibodies by immunoblot analysis.
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bioRxiv - Cell Biology 2024Quote: Antibodies that were used in this study to detect viral and cellular proteins were the monoclonal antibody (MAb) recognizing the FLAG-tag epitope anti-Flag (clone M2, Sigma), the MAb against HCMV pp28 ...
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bioRxiv - Neuroscience 2022Quote: ... After blocking for 90 min with 5% sheep serum (Sigma-Aldrich) at room temperature ...
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bioRxiv - Physiology 2020Quote: ... biotinylated donkey anti-sheep antibody (1:200, Sigma, cat no. AP184B), streptavidin-peroxidase conjugate (1:200) ...
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bioRxiv - Genetics 2019Quote: ... embryos were incubated in working strength sheep anti-digoxigenin rhodamine (Millipore) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... or sheep’s blood (Hemostat DSB100) supplemented with 1mM ATP (Sigma A6419). Meals were warmed to 45°C before being used in the assay ...
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bioRxiv - Cell Biology 2021Quote: ... for sheep antibodies or 3% (w/w) BSA (Sigma-Aldrich, A3059) for other antibodies ...
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bioRxiv - Microbiology 2021Quote: ... A 1:3000 dilution of sheep anti-rabbit HRP conjugate (Sigma) was then added for 1 h ...
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bioRxiv - Biochemistry 2021Quote: ... affinity purified Ubxn7 antibodies or IgG from sheep serum (15131, Sigma);