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Citations for Millipore Sigma :
1 - 50 of 8449 citations for Yellow Fever virus Envelope protein sheep Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Primary antibodies used are as follows: Anti-poly-ADP-ribose binding reagent/ PAR reagent (recombinant protein fused to rabbit Fc tag; Millipore, MABE1031), Rabbit monoclonal anti-Poly/Mono-ADP Ribose (anti-PAR/MAR ...
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bioRxiv - Microbiology 2021Quote: ... the Fc tag of all APN orthologs was removed using thrombin (Millipore Sigma) in a reaction mixture containing ...
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bioRxiv - Immunology 2022Quote: ... pan flavivirus envelope (Millipore MAB10216 ...
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bioRxiv - Cell Biology 2021Quote: Lucifer Yellow Carbohydrazide (Sigma L0259) was prepared as a 1mg.mL-1 stock solution in HBSS with calcium and magnesium (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... yellow pigment monascin (Sigma-Aldrich), and orange pigment rubropuctatin (1717 CheMall Corporation ...
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bioRxiv - Cell Biology 2019Quote: ... 1% Lucifer Yellow (LY; Sigma). Sterile distilled water served as control ...
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bioRxiv - Neuroscience 2023Quote: ... 0.1% Lucifer-Yellow (LY, Sigma), pH 7.3 adjusted with KOH ...
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bioRxiv - Neuroscience 2022Quote: ... The Flag-tag protein affinity sequence (DYKDDDDK; Sigma-Aldrich) was added to the N-terminal to facilitate confirmation of expression ...
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bioRxiv - Molecular Biology 2019Quote: ... Secreted knottin-Fc fusion proteins were purified using Protein A Sepharose (Sigma) followed by size exclusion chromatography (Superdex 75 column ...
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bioRxiv - Bioengineering 2020Quote: ... 0.15% and 0.2% Quinoline yellow (Sigma), 0.1% Tinuvin Carboprotect (a gift from BASF) ...
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bioRxiv - Plant Biology 2019Quote: ... The recombinant proteins with either FLAG-tag or His-tag were purified by corresponding immune affinity agarose beads (Sigma).
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bioRxiv - Immunology 2021Quote: ... Envelope supernatants were immobilized onto ConA (Sigma, catalog no C2272 ...
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bioRxiv - Immunology 2020Quote: ... except anti-flaviviral envelope 4G2 (Merck Millipore), β-Tubulin ...
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bioRxiv - Developmental Biology 2023Quote: ... strep tag was removed from the eluted proteins by thrombin (Sigma) digestion overnight at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... protein tags were removed by tobaccoetchvirus (TEV) protease (#T4455, Sigma-Aldrich) (1:25 w/w ...
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bioRxiv - Immunology 2020Quote: ... and sheep serum (Sigma) was used ...
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bioRxiv - Bioengineering 2021Quote: ... quinoline yellow (QY) were purchased from Sigma-Aldridge (St ...
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bioRxiv - Cell Biology 2022Quote: ... fluorescent yellow‐green latex beads (SIGMA-Aldrich) at a concentration of 8×106 beads/well (∼100 beads/cell ...
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bioRxiv - Immunology 2022Quote: ... alkaline phosphatase yellow (pNPP) liquid substrate (Sigma) and TMB Substrate Reagent Set (BD Pharmingen ...
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bioRxiv - Plant Biology 2022Quote: ... and Fluorol Yellow 088 (FY 088, Sigma). For sequential staining of Nile Red and Calcofluor White ...
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bioRxiv - Developmental Biology 2023Quote: Lucifer Yellow CH dipotassium salt (Sigma, L0144) and Rhodamin Dextran (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Lucifer Yellow (10 µg/ml; Sigma-Aldrich) was added to the pipette solution and intracellular access of the solution was confirmed by excitation at 495 nm and visualization at an emission wavelength of 510 ± 10 nm ...
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bioRxiv - Neuroscience 2024Quote: ... fluorescent yellow-green latex beads (Sigma-Aldrich) were added to iMicroglia cultures treated with or without EVs ...
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bioRxiv - Microbiology 2023Quote: ... latex yellow–green 2 μm beads (Sigma) were coated with human IgM (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... The flow-through from the His-tag cleavage containing the tag free protein was concentrated to 2 mL using Amicon Ultra-4 30k MWCO (Millipore). The concentrated protein was applied to a HiLoad 16/600 S75pg column (GE Life Sciences) ...
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bioRxiv - Molecular Biology 2019Quote: ... was fused with an N-terminal His(6)-tag followed by maltose binding protein (MBP) tag and TEV cleavage site in pET21 vector (Novagen).
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bioRxiv - Neuroscience 2021Quote: ... Fc-tagged and non-Fc-tagged proteins were concentrated using Amicon Ultra 10 kDa MWCO centrifugal filter units (Millipore), dialyzed against PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Germany) and incubated overnight with a polyclonal antibody against Lucifer Yellow (anti-Lucifer Yellow, rabbit polyclonal, Millipore, 1:3000) at 4° C in PBS containing 0.3% Triton X-100 and 5% NGS ...
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bioRxiv - Systems Biology 2021Quote: ... virus protein-bound host proteins were enriched by adding 50 µl anti-HA-agarose slurry (Sigma-Aldrich, A2095) with constant agitation for 3 hours at 4°C ...
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bioRxiv - Genetics 2021Quote: ... Each ORF cloned into the pLVX-EF1a-IRES-PURO/eGFP lentiviral expression vector was added to lentiviral packaging and envelope vectors (Sigma-MISSION-gag-pol and the envelope Sigma-MISSION-VSV-G (Cat ...
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bioRxiv - Biochemistry 2023Quote: ... The His-tag of the eluted protein was cleaved by thrombin (Sigma), incubated at 4 °C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... sheep anti-mouse (Sigma #A5906) or goat anti-rat (Sigma #AP136P ...
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bioRxiv - Developmental Biology 2023Quote: ... and 5% sheep serum (Sigma) diluted in MABT ...
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bioRxiv - Developmental Biology 2023Quote: ... and 5% sheep serum (Sigma) diluted in maleic acid buffer with 0.1% tween-20 (MABT) ...
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bioRxiv - Biochemistry 2023Quote: ... the Siglec-7-Spy Tag-TEV-Fc was concentrated using an AMICON (Molecular Weight Cut Off 30 kDa – Merck Millipore) to approximately 0.15 mg/ml measured via NanoDrop as described above ...
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bioRxiv - Cell Biology 2019Quote: ... S-protein-agarose beads and S-tag antibody were purchased from EMD Millipore. Endo H ...
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bioRxiv - Biophysics 2021Quote: ... Protein was expressed as a His6-tag fusion in BL21(DE3) trxB (Novagen) using autoinduction24 ...
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bioRxiv - Molecular Biology 2020Quote: ... but with a 10% glucose solution containing a yellow dye (1 mg mL−1 Acid Yellow 17; Sigma-Aldrich, St Louis, Missouri) (Ignell et al. ...
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bioRxiv - Bioengineering 2022Quote: ... and tartrazine food dye (Acid Yellow 23, Sigma-Aldrich) at different w/v ratios and combinations using either Hank’s Balanced Salt Solution (HBSS ...
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bioRxiv - Neuroscience 2020Quote: ... 10 mg Lucifer yellow CH di-Lithium salt (Sigma) was dissolved in 1 ml 5 mM KCl solution and filtered prior to use ...
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bioRxiv - Neuroscience 2022Quote: ... sheep anti-TH (Chemicon/Sigma, AB1542), rabbit anti-AVP (19).
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bioRxiv - Neuroscience 2019Quote: ... sheep anti-tyrosine hydroxylase (Millipore AB1542)) 1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Sheep anti-mouse Cy3 (Sigma C2181) and goat anti-rat Alexa Fluor 488 (Invitrogen A11006 ...
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bioRxiv - Neuroscience 2023Quote: ... sheep anti-tyrosine hydroxylase (Millipore AB1542)) 1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... BioLegend),Tyrosine Hydroxylase (sheep, 1:200, Millipore). Secondaries and dilutions are as follows ...
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bioRxiv - Microbiology 2019Quote: ... Fc chimeric proteins were purified via affinity based Fast Protein Liquid chromatography using Prosep® Ultra resin (Millipore). Fc conjugated proteins were eluted with 0.1 M glycine (pH 2.5 ...
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bioRxiv - Genetics 2021Quote: ... Each ORF cloned into the pLVX-EF1a-IRES-PURO/eGFP lentiviral expression vector was added to lentiviral packaging and envelope vectors (Sigma-MISSION-gag-pol and the envelope Sigma-MISSION-VSV-G (Cat: CST-DNA Sigma, St-Louis, MO)) in a 2:2:1 ratio and co-transfected into HEK-293T packaging cells via the calcium phosphate precipitation method according to the Open-Biosystem protocol (as described in (23)) ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was eluted with elution buffer until resin no longer appeared yellow and concentrated to 1 mL in a 3K protein concentrator (Millipore Sigma). Concentrated protein was purified by SEC as described above.
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bioRxiv - Immunology 2022Quote: ... Virus was concentrated from media using PEG Virus Precipitation Kit (Sigma). Viral titer was determined by QuickTiter™ Lentivirus Associated HIV p24 Titer Kit (Cell Biolabs ...
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bioRxiv - Cell Biology 2022Quote: ... and the GST tag was cleaved by incubating the protein with Thrombin (Sigma Aldrich) overnight at 4 °C ...