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Citations for Millipore Sigma :
101 - 150 of 10000+ citations for U3 Small Nucleolar RNA Associated Protein 4 Homolog CIRH1A Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 500 µg of protein was incubated overnight at 4°C with primary antibody as indicated (anti-AHCY [MBL RN126PW], anti-HA [Millipore 05-904] ...
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bioRxiv - Genetics 2020Quote: ... Samples were immunoprecipitated with antibodies (5-8 μg) overnight at 4°C followed by incubation for 3 hours with protein G-sepharose beads (Millipore) and washed sequentially ...
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bioRxiv - Genetics 2020Quote: ... and precipitated with antibodies overnight at 4°C followed by incubation for 3 h with protein G Sepharose beads (Millipore). Normal mouse ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mg of proteins was used to performed incubation overnight at 4°C with 5 μg of anti-Flag mouse antibody (Sigma) in 250 μL of complemented non-denaturing lysis buffer ...
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bioRxiv - Cell Biology 2021Quote: ... 18 to 24 hrs after transfection the cells were lysed and incubated with CycB1 antibody overnight at 4°C and Protein G Sepharose beads (Sigma) were added to each sample for 3.5 hours at 4°C after the overnight incubation with antibody ...
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bioRxiv - Neuroscience 2019Quote: ... lysates of tissues or tells were incubated with antibodies overnight (4°C) and subsequently with protein G-agarose beads (Millipore) for 5h (4 °C) ...
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bioRxiv - Molecular Biology 2019Quote: ... Sonicated lysates were pelleted for 5 min at 14000 rpm in a microcentrifuge and the entire supernatant was transferred to a new microfuge tube and incubated overnight at 4°C with 5 µg of anti-Myc antibody (9E10) and 20 µl of protein G magnetic beads (Millipore). Following IP ...
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bioRxiv - Genetics 2020Quote: ... and precipitated with antibodies overnight at 4°C followed by incubation for 2 h with protein G Sepharose beads (Millipore). Normal mouse or rabbit IgG (Santa Cruz ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with the primary antibodies of interested proteins at 4°C overnight (Nestin, 1:1000, Sigma; Tuj1, 1:1000, Sigma; GFAP ...
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bioRxiv - Cell Biology 2019Quote: ... Protein G Sepharose 4 Fast Flow (P3296 Sigma-Aldrich) were incubated with 2 µg of GFP antibody raised in Goat for 2h at 4°C and were then washed with lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Colitis-associated CRC was induced by injecting AOM (10 mg/kg, Sigma), followed by three cycles of 2% DSS treatments.
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bioRxiv - Bioengineering 2022Quote: MSC derived small EVs were characterized for protein concentration using the bicinchoninic acid (BCA) assay (Sigma Aldrich, Oakville, ON, Canada). Nanoparticle tracking analysis was performed using ZetaView (Particle Metrix ...
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bioRxiv - Microbiology 2021Quote: ... the rNA concentration and a 4-methylumbelliferone (Sigma) standard curve.
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bioRxiv - Cell Biology 2022Quote: ... thereafter differentiations were kept in RB- without small molecule inhibitors until day 3 (d3) before the WNT inhibitor IWR-1 (4 μM, Sigma, #I0161) was added for 48 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were trypsinized into small cell clusters and incubated with the concentrated virus particles supplemented with 4 μg/mL Polybrene (Sigma Aldrich), for 60 minutes while centrifuging at 600 rpm at RT followed by 4 hr incubation at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... hippocampi were minced into small pieces and homogenized in 4 ml ice-cold nuclei EZ lysis buffer (#NUC-101, Millipore Sigma) supplemented with 1×Halt protease inhibitor cocktail (ThermoFisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... A small piece of the gonad was immediately stored in an Eppendorf with 1 ml of RNA later (Sigma) at 4°C for 24 hours and then ...
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bioRxiv - Molecular Biology 2019Quote: ... or chemically crosslinked (small RNA blots) by incubation with 0.16 M l-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, Sigma) in 0.13 M 1-methylimidazole (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... pLKO.1-nontarget small hairpin RNA (shRNA) control vector (SHC002) and pLKO.1-shSOCS6 (TRCN0000284351) were purchased (Sigma Aldrich).
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bioRxiv - Microbiology 2024Quote: Total RNA from small intestine was isolated from liquid nitrogen frozen and ground tissues with TRIZOL regent (Sigma, USA). Equal amounts of total RNA were used to synthesize cDNA with the PrimeScript™ RT reagent Kit with gDNA Eraser (Takara RR047A ...
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bioRxiv - Genomics 2021Quote: ... 4 µg H3K4me1 antibody (Millipore 07-436), 2 µg H3K4me3 antibody (Active Motif #39915) ...
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bioRxiv - Molecular Biology 2023Quote: Antibodies: 4 μg COREST (Millipore, 07-455), 4 μg USP22 (Santa Cruz ...
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bioRxiv - Immunology 2023Quote: ... 4 μg of anti-Flag antibody (Sigma) and 50 μl of A/G beads (Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... 4 µg of Flag M2 antibody (Sigma) and 1 µg of Spike-in antibody (Active Motif) ...
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bioRxiv - Biochemistry 2020Quote: ... A small amount of glass beads (Sigma) were added and cells were lysed by three rapid freeze-thaw-vortex cycles ...
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bioRxiv - Microbiology 2022Quote: ... small molecule SOX18 inhibitor SM4 (Sigma-Aldrich / or a kind gift from Gertrude Biomedical Pty Ltd. ...
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bioRxiv - Biophysics 2023Quote: ... Small molecules (L-DOPA (Sigma-Aldrich, D9628), L-Tyr (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... Small molecule: Serotonin (H9523, Sigma-Aldrich, UK). Ampure XP magnetic beads (for purification of DNA barcoded probes ...
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bioRxiv - Cell Biology 2019Quote: ... The lysates were incubated with primary antibodies for overnight at 4°C and then protein A/G magnetic beads (Millipore, USA) were added for 2 h at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were adjusted to 1 mg/ml in lysis buffer and subject to overnight immunoprecipitation at 4°C with either USP7 or USP11 antibody and protein A agarose beads (Sigma-Aldrich), or with GFP-NanoTrap beads (prepared in house ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Genetics 2020Quote: ... Samples were immunoprecipitated with S9.6 antibodies (8 μ antibody/ 5μ DNA) overnight at 4°C followed by incubation for 3 hours with protein G-Sepharose beads (Millipore) and washed sequentially ...
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bioRxiv - Immunology 2023Quote: ... All antibodies were incubated separately at a concentration of 10 μg/ml in tubes together with 330 ng/ml HIS-tagged CD40 recombinant protein and 4 μg/ml peroxidase-coupled anti-HIS detection antibody (Sigma-Aldrich) for 60 minutes in ELISA buffer (PBS ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were snap-frozen in small aliquots after concentrating them in this buffer using a Centricon 50 (EMD Millipore, Billerica, MA). Protein concentrations were determined using BSA standards on a Coomassie-Blue-stained SDS-PAGE gel.
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bioRxiv - Neuroscience 2022Quote: Fixation was performed following 24 hrs of recombinant protein treatment using 4 % PFA / 4% Sucrose (Sigma) in 1x PBS at RT for 15 min ...
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bioRxiv - Molecular Biology 2019Quote: ... the proteins were concentrated with Amicon Ultra 4 MWCO10kDa (Millipore) to approximately 3 – 4 mL ...
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bioRxiv - Molecular Biology 2019Quote: The protein was concentrated with Amicon Ultra 4 MWCO30kDa (Millipore) at 20 °C at 1650 × g ...
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bioRxiv - Cell Biology 2021Quote: ... 50 µl of Protein G Sepharose 4 Fast Flow (Sigma) slurry were washed ...
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bioRxiv - Microbiology 2020Quote: ... Protein was concentrated using Amicon Ultra-4 30k MWCO (Millipore). 3C protease was added to purified protein in a 1:50 (w:w ...
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bioRxiv - Molecular Biology 2022Quote: ... Purified proteins were concentrated by ultrafiltration (Amicon Ultra-4, Millipore) and diluted in PBS.
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bioRxiv - Biochemistry 2023Quote: ... The proteins were reduced with 4 mM DTT (Sigma-Aldrich) for 60 min at room temperature and alkylated in the dark using 8 mM iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2019Quote: ... and senescence by senescence-associated β-galactosidase (SA-β-gal) staining (Sigma-Aldrich). Immunofluorescence was performed according to standard protocols ...
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bioRxiv - Microbiology 2021Quote: ... in CK+DCI with 10 μM Rho-associated kinase inhibitor (Sigma Y-27632). At 48h after plating ...
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bioRxiv - Molecular Biology 2019Quote: ... Small RNAs were crosslinked to the membrane by chemical crosslink using N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC, Sigma-Aldrich). Membrane was pre-hybridized for 20 min with PerfectHyb(tm ...
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bioRxiv - Microbiology 2021Quote: The following small molecule inhibitors and antibodies were used for TEER and EGL inhibition assays: ATN-161 (Sigma, SML2079), SB 431542 hydrate (Sigma ...
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bioRxiv - Biophysics 2022Quote: ... and mouse monoclonal primary antibody against myelin basic protein antibody (Millipore) at 1:200 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... Cenexin rabbit antibody (Protein tech) and centrin mouse antibody (EMD Millipore) diluted 1:1000 for centrin and 1:200 for other centrosomal proteins in 1% BSA in PBS for 1.5 hours at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue sections were incubated overnight at 4°C with primary antibodies diluted in antibody solution containing 4% NDS (Sigma-Aldrich) and 0.1% Triton-X (Sigma- Aldrich ...
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bioRxiv - Plant Biology 2020Quote: ... antibodies and Protein A agarose beads (Sigma). After elution of immune complexes ...