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Citations for Millipore Sigma :
51 - 100 of 10000+ citations for U3 Small Nucleolar RNA Associated Protein 4 Homolog CIRH1A Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Lgr5-EGFP-IRES-creERT2/RCL-tdT small intestinal organoids were treated in vitro with 100 nM of 4-Hydroxitamoxifen (4-HT) (Sigma) for 48 h to induce the expression of tdTomato in Lgr5+ cells ...
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bioRxiv - Biochemistry 2022Quote: ... protein induction was performed on a small scale (2mL) and cells were disrupted using BugBuster protein extraction reagent (Novagen).
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bioRxiv - Cell Biology 2022Quote: ... Small molecules/ proteins were added to extracts as follows: 1 µM MLN8237 (Sigma-Aldrich) (or 0.5% DMSO ...
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bioRxiv - Cancer Biology 2022Quote: ... The lentivirus containing small hairpin RNA (shRNA) (Sigma, Mission Human Genome shRNA Library) against the target gene was inoculated in the presence of 8μg/ml polybrene (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... Bound FLAG-HA-tagged KAT6B and associated proteins were eluted with 0.3 μg/μl 3x FLAG tag peptide (Sigma) in BC100 buffer for 1 h with gentle rotation at 4°C.
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bioRxiv - Developmental Biology 2019Quote: ... into single-cell suspension and re-suspended in E8 media containing 10 μM Rho-associated protein kinase inhibitor (Sigma). Approximately 100,000 cells were re-plated into Matrigel-coated 6-well plates ...
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bioRxiv - Cell Biology 2021Quote: ... The protein complexes associated with p53 were resolved by SDS-PAGE and transferred onto a PVDF membrane (#IPVH00010, Millipore). The membrane was blocked with 3% BSA in PBS for 1 h at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... RNA-YTHDC1-antibody complexes were pulled down using Dynabeads Protein A/G (Millipore) and washed 3 times with NETN100 buffers (20 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies were purified from hybridoma culture supernatant using protein G Sepharose 4 Fast Flow (Sigma). Injection of the respective antibody resulted in depletion of >90% of CD4+ or CD8+ T cells for at least 14 days ...
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bioRxiv - Neuroscience 2021Quote: ... myelin associated glycoprotein (#MAB1567; MAG; Sigma; 1:500), and the CSPG brevican (#SC-135849 ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... and purified small RNA was eluted from the beads in Tri-Reagent (Sigma-Aldrich) following the manufacturer’s instructions.
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bioRxiv - Genomics 2019Quote: ... RNA-protein complexes were incubated with antibody-bound Protein A Dynabeads (anti-FLAG [Sigma Aldrich F3165] or anti-IgG) at 4C for 2 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were stained overnight at 4°C with primary antibody (Mouse anti-γH2AX antibody; Millipore Sigma #05-636, 1:300) diluted in blocking solution ...
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bioRxiv - Developmental Biology 2022Quote: ... samples were suspended in small quantities of a 4 mg/ml solution of BSA (Sigma, USA) in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... 4 µg of antibodies and 20 µL of Magna Chip Protein A/G magnetic beads (Millipore) were added and samples were incubated overnight on a rotating wheel at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... A small fraction of m7G*ppp-RNA and of G*ppp-RNA were digested with 5 μg nuclease P1 (Sigma) in 50 mM NaOAc pH5.2 buffer to originate the m7G*ppp and G*pppG markers in the thin-layer chromatography (TLC ...
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bioRxiv - Immunology 2023Quote: ... were formed by dissociating and maintaining PSCs in ESC media supplemented with 10 μM Rho-associated protein kinase (ROCK) inhibitor (Sigma, Y0503) while being kept on an orbital shaker at 100 rpm for 6 days to allow for spontaneous formation of EBs with hematopoietic potential ...
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bioRxiv - Immunology 2021Quote: Small interfering RNA (siRNA) to human 3’ UTR PKC-θ was purchased from Sigma Aldrich. YTS-2DL1 cells were transfected with siRNA specific for the indicated genes ...
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bioRxiv - Plant Biology 2020Quote: ... Small RNA was extracted as per protocol using the mirPremier microRNA Isolation Kit (Sigma-Aldrich), then DNAse treated (Brouwer et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... Nrf2 endoribonuclease-prepared small interfering RNA (esiRNA) was purchased from Sigma-Aldrich (St. Louis, MO). Kits for RNA isolation and QIAshredder were purchased from Qiagen (Valencia ...
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bioRxiv - Cancer Biology 2019Quote: ... Mouse small hairpin RNAs (shRNAs) for Cep55 (pLKO plasmids, (Sigma Aldrich®, St Louis, USA)) clones were established using lentiviral packaging using PEI (Poly -ethyleneimine ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then incubated with primary antibody overnight at 4 °C [1:500 goat anti-RNA polymerase II antibody (PLA0292, Sigma) with 1:500 rabbit anti-PCNA antibody (PLA0079 ...
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bioRxiv - Molecular Biology 2021Quote: Protein-G (Protein G Sepharose® 4 Fast, cat# GE17-0618-01) or Protein-A (ProteinA-Sepharose® 4, cat#P9424 Millipore) beads were washed twice with 1x PBS and twice with IP100 buffer (25 mM Tris-HCl 7.9 ...
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bioRxiv - Microbiology 2020Quote: ... Resulting primary cells were first cultured in the selective media to proliferate in the presence of the Rho-associated protein kinase (ROCK) inhibitor (Y-27632, 10 µg/ml, Sigma-Aldrich, Poland) (95) ...
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bioRxiv - Cancer Biology 2019Quote: Small interfering RNAs (siRNA) targeting ATP7B and ATP7A were purchased from Sigma-Aldrich (St. Louis, USA). Scrambled siRNAs were used as a negative control ...
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bioRxiv - Microbiology 2021Quote: ... All PEG and associated chemical formulations were obtained from Sigma and purchased as chemical compounds ...
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β-catenin programs a tissue-specific epigenetic vulnerability in aggressive adrenocortical carcinomabioRxiv - Cancer Biology 2022Quote: ... and associated wash buffers (Sigma-Aldrich, Cat. No. DUO82047-4L) and PLA probes according to manufacturer protocol with modifications to blocking and primary antibody incubation steps ...
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bioRxiv - Neuroscience 2023Quote: ... small-worldness (indicated by Sigma) showed marked variation over the complete range of thresholds (1% – 90% connection density ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA-protein complexes were immunoprecipitated with 12 μg anti-FLAG M2 antibody (Sigma-Aldrich, Cat #F1804) or 8 μg anti-U2AF2 antibody (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... mediated knockdown of Zfp804a (the rat homolog of ZNF804A) in rat primary cortical neurons was conducted using the N-Ter Nanoparticle siRNA Transfection System (Sigma: N2788) at DIV15 per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Dendrites were identified using a staining against the microtubule-associated-protein-2 with Rabbit-anti-MAP2 PAB (Milipore Sigma, cat. AB5622, 1:1000) and GAR-STAR488 SAB (Abberior ...
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bioRxiv - Pathology 2021Quote: ... The DNP signal associated with RyR was determined using a specific anti-DNP antibody according to the manufacturer’s instructions (Millipore, Billerica, MA). All immunoblots were developed and quantified using an Odyssey system (LI-COR Biosciences ...
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bioRxiv - Genetics 2019Quote: Small-interfering RNAs (siRNA) targeting human C/EBPβ were purchased from Sigma Aldrich (St Louis, MO, USA). siRNA ID ...
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bioRxiv - Cell Biology 2022Quote: ... C2C12 myoblasts were transfected with plasmids encoding small hairpin (sh)-RNA targeting Adamtsl2 (Mission shRNA, Sigma Aldrich) using PEI ...
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bioRxiv - Neuroscience 2019Quote: ... minced into small pieces and homogenized in 4 ml ice-cold nuclei EZ lysis buffer (#NUC-101, Millipore Sigma) supplemented with 1X HaltTM protease inhibitor cocktail (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... the small intestine samples were placed into either 4% PFA in PBS (Sigma-Aldrich Corporation, St. Louis, MO, USA) or Carnoy’s fixative ...
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bioRxiv - Developmental Biology 2021Quote: ... LIF has been omitted and derived EpiSCs can be passaged 1:4–1:10 every by triturating the colonies into small clumps using 0.5 mg ml–1 collagenase IV (Sigma). To derive mesodermal progenitors utilizing the T-eGFP FoxA2-tagRFP double knock-in reporter cell lines ...
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bioRxiv - Microbiology 2019Quote: ... 4 µg of MBP (Myelin Basic Protein) (Sigma) and 4 µg of CstK were used ...
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bioRxiv - Bioengineering 2020Quote: ... 3) Rho-associated kinase (ROCK) inhibitor Y27632 (10 μM; Sigma-Aldrich), 4 ...
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bioRxiv - Cell Biology 2021Quote: ... the Rho-associated kinase (ROCK) inhibitor Y27632 (10 μM; Sigma-Aldrich), the actin de-polymerizer latrunculin B (10 μM ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we employed antibodies against RNA-Binding Protein RBFOX3 (aka NeuN; mouse anti-NeuN, Alexa488-conjugated, MAB377X, Millipore) that is expressed in all neuronal nuclei ...
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bioRxiv - Molecular Biology 2021Quote: ... The blots were incubated with primary antibodies to the following proteins overnight at 4 °C: (1) CTCF (1:1,000; 07-729, Merck Millipore), (2 ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with the primary antibodies of interested proteins at 4°C overnight (Nestin, 1:1000, Sigma; Tuj1 ...
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bioRxiv - Cell Biology 2023Quote: The small molecule MDL-800 (Sigma) was used at a concentration of 20 μM to activate SIRT6 ...
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Deep Plasma Proteome Profiling by Modulating Single Nanoparticle Protein Corona with Small MoleculesbioRxiv - Bioengineering 2024Quote: ... Small molecules were ordered from Sigma, Abcam ...
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bioRxiv - Pathology 2022Quote: ... The cells were transfected with different types of CYP24A1-specific small-hairpin RNA (shRNA)-expressing lentivirus plasmids (Sigma) using FuGENE6 (Roche ...
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bioRxiv - Immunology 2023Quote: ... small interfering RNAs (siRNAs) and scrambled controls (scRNAs) designed for astikine and hemocytin were synthesized by Millipore Sigma with UU overhangs ...
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bioRxiv - Neuroscience 2021Quote: ... Larva motion was recorded at 300 Hz with an IR-sensitive monochrome camera (Flir, GS3-U3-41C6NIR-C) equipped with a long-pass filter and a photo objective (Sigma Zoom, 18-200 mm). Each larva received 10 minutes of stationary grating followed by 5 s moving grating and 5 s stationary grating for 10 minutes ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... Bound proteins were captured with Protein G agarose for 4 h (Sigma-Aldrich, #17061801), spun at low speed ...
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bioRxiv - Neuroscience 2022Quote: Previous research has indicated that a differential microtubule-associated protein 2 (MAP2) immunolabeling pattern can distinguish dense-core amyloid plaques from diffuse amyloid plaques using DAB (Sigma-Aldrich, St. Louis, MO, USA) as a chromogen (74).