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Citations for Millipore Sigma :
201 - 250 of 287 citations for Toxocara Canis Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2022Quote: ... Enzymatic antigen retrieval was performed for 10 minutes in a 37°C water bath using pepsin (Sigma-Aldrich, P7000). After rinses ...
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bioRxiv - Developmental Biology 2023Quote: ... 5μm E14.5 paraffine sections underwent deparaffinization and microwave antigen retrieval in pH 6.0 sodium citrate with 0.05% Tween-20 solution (Sigma-Aldrich), followed by gradual chilling ...
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bioRxiv - Microbiology 2023Quote: Sections were deparaffinized in xylene and rehydrated in a descending ethanol series prior to heat-induced antigen unmasking in Citrate Buffer pH 6.0 (Sigma). After blocking of nonspecific binding in PBS-T containing 10% goat serum and 1% BSA ...
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bioRxiv - Developmental Biology 2023Quote: ... Tissue cross-sections were deparaffinized and rehydrated and subjected to antigen retrieval using citrate buffer (0.0126 M citric acid, Cat. No. C-0759, Sigma; 0.0874 M sodium citrate ...
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bioRxiv - Bioengineering 2023Quote: ... Sections (5 μm) were deparaffinized and rehydrated and subjected to antigen retrieval using citrate buffer (0.0126 M citric acid, Sigma cat ...
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bioRxiv - Bioengineering 2023Quote: ... The antigen-retrieval buffer used for live-cell lysis was 2× Tris-glycine buffer (Sigma Aldrich, St. Louis, MO) supplemented with 1% SDS and 12 M urea (Sigma Aldrich ...
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bioRxiv - Genetics 2023Quote: Rehydrated histological sections underwent antigen retrieval by incubation with 1 mg/mL pepsin in 0.5 M acetic acid (Millipore) at 37 °C for 30 min ...
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bioRxiv - Immunology 2021Quote: ... MAb was reacted with antigen coated on the plates and then treated with serially diluted C1q from human serum (Sigma). C1q binding was detected with horseradish peroxidase-conjugated anti-C1q antibody ...
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bioRxiv - Microbiology 2022Quote: ... immunization was continued in bi-weekly intervals by subcutaneous and intraperitoneal injection of 20-30 μg of antigen preparations formulated with Ribi Adjuvant System (RAS, Sigma) according to manufacturer instructions ...
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bioRxiv - Microbiology 2020Quote: ... Mice were injected intraperitoneally with 100 µL chyle containing 10-50 µg antigen prepared with TiterMax Gold adjuvant (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... except that the primary antibody for viral detection was a monoclonal antibody against the coronavirus group antigen (Milipore Sigma #MAB9013) and the secondary was a horseradish-peroxidase-conjugated goat-anti-mouse antibody (enQuire BioReagents # Q2AB1).
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bioRxiv - Microbiology 2022Quote: ... the Milliplex SARS-CoV-2 Antigen Panel 1 IgG was used according to the manufacturer’s instructions (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Developmental Biology 2021Quote: ... sections were subject to 0.1M citrate antigen retrieval at 95°C for 10 mins before the primary antibody for type-II collagen (Millipore, MAB8887), ACAN [30] or lubricin (Abcam ...
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bioRxiv - Immunology 2021Quote: ... Cocalico Biologics administered vaccines by first priming with 50µg of either the single-antigen cocktail of nanoparticles or mosaic nanoparticles in TiterMax Gold (Sigma-Aldrich) and boosting with 25µg of either for-mu-lation every 3 weeks for a total of 3 boosts after prime ...
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bioRxiv - Immunology 2021Quote: ... with 1% milk and incubated for 2 hours with the mixture of antigen-coupled microsphere beads in 96-well filter bottom plates (Millipore). After washing with assay buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... Cryostat sections or whole embryos were stained using standard protocols and antibodies against the following antigens: HA (1:250, Sigma), β-catenin (1:300 ...
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bioRxiv - Immunology 2022Quote: ... with 1% milk and incubated for 2 hr with the mixture of antigen-coupled microsphere beads in 96-well filter bottom plates (Millipore). After washing three times with assay buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... Blocking of unspecific antigens was carried out by incubation in 3% bovine serum albumin (BSA; Sigma-Aldrich; dissolved in PBST) for 1 h at RT ...
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bioRxiv - Synthetic Biology 2022Quote: ... The resulting cells were used to prepare O78 LPS antigen in house by hot phenol water extraction after DNase I (Sigma) and proteinase K (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... and antigen retrieval at 98°C for 15 minutes (KOS microwave tissue processor, Milestone) in citrate buffer pH6.0 (Sigma, C999). Prior to staining ...
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bioRxiv - Biochemistry 2022Quote: ... Then each membrane was incubated with the corresponding primary antibody (mouse IgG anti-Lewis antigen, Sigma–Aldrich, 1:500 dilution) for 1h at 4°C ...
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bioRxiv - Immunology 2022Quote: ... with 1% milk and incubated for 2 hours with the mixture of antigen-coupled microsphere beads in 96-well filter bottom plates (Millipore). After washing with assay buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... For the BrdU staining protocol on tissue section a DNA hydrolysis step directly after antigen retrieval was performed by the addition of 2M HCL (Sigma) in PBS for 30 minutes at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were subsequently rinsed with cold running tap water for 5 min followed by antigen retrieval using citrate buffer at pH 6.0 (Sigma Aldrich). Afterwards ...
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bioRxiv - Immunology 2023Quote: ... parietal-cell antigens were prepared from murine gastric corpus which was digested with 0.05 mg/mL Liberase DH (LIBTM-RO; Sigma-Aldrich) and 0.1 mg/mL DNase I (Roche) ...
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bioRxiv - Cell Biology 2023Quote: ... washed in PBS/0.1 % Triton X-100 and treated with antigen retrieval solution [proteinase K 20 mg/ml (Sigma-Aldrich) dissolved in 10 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... with 1% milk and incubated for 2 hours at room temperature with the mixture of antigen-coupled microsphere beads in 96-well filter bottom plates (Millipore). After washing three times with assay buffer ...
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bioRxiv - Neuroscience 2023Quote: ... spinal cord sections underwent antigen retrieval at 85 °C for 10 min with 1X citrate buffer pH 6.0 (Sigma-Aldrich). Tissue sections were washed with PBS and permeabilised with 1% Triton-X-100 in PBS for 30 min at RT ...
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bioRxiv - Physiology 2024Quote: ... followed by rehydration with different % of ethanol (From 100 % to 70 %, 111000190, Pharmco) and the antigen retrieval step with citric buffer (pH 6, C9999, Sigma) in heat for 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... hydrated sections were subjected to antigen retrieval by pronase (1 mg/ml in PBS containing 5 mM calcium chloride; Sigma-Aldrich) incubation for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: Soluble egg antigen (SEA) of LE was extracted by mechanical lysis (Funakoshi motorized pestle; Diagnocine, Hackensack, NJ) in RIPA buffer (Millipore Sigma) supplemented with protease inhibitors (Protease Inhibitor Cocktail I ...
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bioRxiv - Immunology 2022Quote: ... Sections were washed 3 times in PBS followed by antigen retrieval for 20 minutes with steam using 1X Citrate buffer (Millipore, 21545), pH=6.0 ...
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bioRxiv - Immunology 2022Quote: ... Slides were subsequently boiled with antigen retrieval buffer (Tris-EDTA, pH9, made in house or citrate buffer, pH6, Sigma C999-1000mL) and blocked for one hour with PBS containing 1% bovine serum albumin (BSA) ...
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bioRxiv - Cancer Biology 2019Quote: ... the TMA slide was subjected to Cell Conditioning 1 (CC1, Ventana) antigen retrieval and stained with a prediluted anti-MCT antibody (Mouse, Clone G3, Millipore #MAB1222) using an automated immunostainer ...
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bioRxiv - Immunology 2022Quote: ... Endogenous peroxidase activity was blocked with 3% hydrogen peroxide for 10 min and antigen retrieved using proteinase K (Sigma-Aldrich, P6556) in Tris-EDTA pH8 buffer for 4 min at room temp ...
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bioRxiv - Developmental Biology 2021Quote: ... boiled in citric acid (pH 2.0) for antigen retrieval and stained with an α-MHC antibody (Sigma My-32, 1:1000) or α-GFP antibody (Abcam ab13970 ...
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bioRxiv - Microbiology 2020Quote: Infected cells grown on coverslips were fixed with 4% paraformaldehyde and permeabilized/blocked with a Triton-X100 (0.2%)/BSA (3%) solution and then incubated with mouse anti-Flavivirus Group Antigen 4G2 (Millipore, Burlington, MA), mouse anti-alphavirus capsid (kindly provided by Dr ...
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bioRxiv - Physiology 2020Quote: ... 5 μm tissue sections were subject to antigen retrieval (0.01 M citric acid and 0.1% Triton X) (Sigma, St. Louis, MO), blocked with the avidin/biotin blocking kit (Vector Laboratories) ...
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bioRxiv - Molecular Biology 2021Quote: ... Anti-flavivirus group antigen antibody that probes ZIKV E protein and anti-SARS-CoV-1/2 NP 1C7C7 antibody was purchased from Sigma-Aldrich. Anti-influenza A virus nucleoprotein (NP ...
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bioRxiv - Cancer Biology 2020Quote: ... Tissue sections were subjected to a heat-induced antigen retrieval step prior to exposure to primary OSM antibody (1:50, HPA029814, Sigma-Aldrich). Immunodetection was performed using the Roche Ventana BenchMark ULTRA IHC/ISH staining system ...
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bioRxiv - Molecular Biology 2020Quote: ... pH 8 and the α-NOCT antibody was antigen affinity purified with NOCT (64-431) immobilized on Immobilon-P PVDF membrane (Millipore). Guinea pig α-NOCT was eluted from the membrane with 0.1 M glycine and 20% glycerol ...
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bioRxiv - Genetics 2022Quote: Longitudinal paraffin-embedded sections were processed as described (Gomez et al., 2022) following 1-hour antigen retrieval with 2mg/ml Hyaluronidase (Sigma-Aldrich) at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (clone PAb416, Sigma Aldrich - Saint-Quentin-Fallavier France) diluted 1 ...
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bioRxiv - Developmental Biology 2023Quote: ... rat anti-PDGFRβ (eBiosciences 14-1402-81), rabbit anti-AQP3 (Alomone Labs, AQP-003, after antigen retrieval) rabbit anti-Runx1 (Sigma, HPA004176), rabbit anti-IRF9 (Cell Signalling ...
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bioRxiv - Genetics 2023Quote: ... Antigen retrieval was performed by pressure cooker incubation of slides in 1L of pre-warmed 1X Citrate Buffer (Sigma Aldrich #C9999) + 0.1% Tween in dH2O for a total of 15 mins (human ...
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bioRxiv - Microbiology 2023Quote: ... at 15 days and 36 days with 1:1 (v/v) mixture of antigen with incomplete Freund’s adjuvant (Sigma-Aldrich, USA). After the first immunization ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue sections were immediately subjected to heat-mediated antigen retrieval at 95°C for 10 minutes in 1X pH6.0 sodium citrate buffer (Sigma-Aldrich, #21545) using Micromed T/T Mega Multifunctional Microwave Rapid Histoprocessor (Milestone) ...
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bioRxiv - Immunology 2023Quote: Eosinophilic airway inflammation was induced by two intraperitoneal injections of 5μg of cockroach antigen (CA, Greer Laboratory, Lenoir, NC) mixed in 100μl of alum adjuvant (aluminum hydroxide, Sigma, St. Louis, MO) at day 0 and 7 ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by antigen retrieval using Tris EDTA buffer pH 9 (Genemed, 10-0046) or 1x sodium citrate at pH 6 (Sigma, C9999) according to manufacturer’s recommendation for specific antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... coverslips were first incubated with Tris-glycine solution 5min at room temperature followed by blocking of non-specific antigens with a cold-water fish blocking buffer (Sigma-Aldrich) for 30min ...