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Citations for Millipore Sigma :
151 - 200 of 287 citations for Toxocara Canis Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Immunofluorescence assays were performed after deparaffination and antigen retrieval in citrate buffer pH6.0 (Sigma Aldrich). The Click-iT AF488 (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... for 30 minutes and the antigen-antibody reaction was visualized using di-aminobenzidine (DAB, Sigma) as the chromogen ...
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bioRxiv - Immunology 2024Quote: ... Antigen-reactive mAbs were detected with goat anti-human IgG (Fab)-horseradish peroxidase (Sigma-Aldrich) conjugate diluted in PBS at a ratio of 1:5000 ...
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bioRxiv - Cell Biology 2024Quote: ... Antigen retrieval was performed by heating the slides in 1x Citrate buffer pH 6 (Sigma) for 40 min ...
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bioRxiv - Cell Biology 2020Quote: ... Antigen retrieval was carried out with 0.2 U/mL Chondroitinase ABC (C2905, Sigma, Merck, Darmstadt, Germany) at 37 °C for two hours for DCN ...
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bioRxiv - Microbiology 2020Quote: ... was used to detect the different antigens with anti-rabbit secondary antibodies (1:25000) (Sigma-Aldrich).
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bioRxiv - Physiology 2020Quote: ... sections were dewaxed and rehydrated prior to enzymatic antigen retrieval (trypsin from porcine pancreas (T7168, Sigma)) ...
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bioRxiv - Neuroscience 2021Quote: ... Heat-mediated antigen retrieval was performed by placing slides in 1x sodium citrate buffer (PHR1416, Sigma), pH 6.0 ...
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bioRxiv - Immunology 2023Quote: ... Diphtheria antigen samples were prepared by desorption with sodium citrate (Sigma-Aldrich Chemie GmbH Taufkirchen, Germany) [23] ...
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bioRxiv - Immunology 2023Quote: ... TUNEL staining was conducted following antigen retrieval using the In Situ Cell Death Detection Kit (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... antigen retrieval was performed in 10 mM sodium citrate buffer (pH 6.0; S1804-500G, Sigma-Aldrich) for 12 min at in a microwave at circa 97°C
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bioRxiv - Cancer Biology 2023Quote: ... rehydrated with an alcohol series and incubated in Tris-EDTA antigen retrieval buffer (#E1161, Sigma-Aldrich) for 20 minutes in a pressure cooker at 95°C ...
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bioRxiv - Physiology 2024Quote: ... and distilled water followed by heat-induced antigen retrieval in citrate buffer (cat# C9999, Sigma-Aldrich) using a 2100 Retriever (Electron Microscopy Sciences) ...
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed by submerging slides in boiling 10 mM sodium citrate buffer (Sigma-Aldrich), before washing with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... The seven target proteins and antibodies for the sFANSeq panel included Neuronal Nuclei Antigen (NeuN, Millipore Cat# ABN91 ...
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bioRxiv - Immunology 2022Quote: ... were coated with respective antigen for 2h at RT and then blocked with 5% BSA (Sigma-Aldrich). 50 μl 1:50 diluted plasma sample or monoclonal Abs was added to the wells and incubated overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... MEFs were immortalized with SV40 large T-antigen by transfection using GeneJuice transfection reagent (Merck Millipore 70967).
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bioRxiv - Cell Biology 2020Quote: ... Antigen retrieval was performed by microwaving sections in preheated 0.01 M citrate buffer pH 6 (Sigma Aldrich) for 5 min.
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bioRxiv - Biochemistry 2020Quote: ... the antigen was detected using mouse-anti-polyHis conjugated with horseradish peroxidase (HRP) (1:20000, A7058, Sigma) for His-tagged antigens ...
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bioRxiv - Biochemistry 2020Quote: ... for mFc tagged antigen versions or goat-anti-hIgG(Fc) conjugated with HRP (1:70000, A0170, Sigma) if hFc-tagged antigens had to be detected ...
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bioRxiv - Molecular Biology 2022Quote: ... Antigen-antibody complexes were identified using goat anti-mouse AP (1:10,000 dilution of A2179, Sigma-Aldrich) and CDP-Star™ Substrate (T2146 ...
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bioRxiv - Developmental Biology 2022Quote: ... antigen unmasking was performed either in 10 mM sodium citrate buffer (pH 6.0; S1804-500G, Sigma-Aldrich) for 12 min at 97°C ...
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bioRxiv - Neuroscience 2023Quote: ... underwent antigen retrieval for 30 minutes at 98°C in citrate buffer (10 mM citrate, Sigma-Aldrich) 0.05% Tween 20 (pH 6.0 ...
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bioRxiv - Developmental Biology 2023Quote: ... antigen retrieval was performed by boiling the sections in 10 mM sodium citrate and 0.05% Tween20 (Sigma) for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... the antigen-serum-bead mixture was transferred into a 96-well filter HTS plate (Millipore, Bedford, MA). The plate was washed 8 times with Buffer A followed by twice with PBS on a vacuum manifold as described previously [26] ...
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bioRxiv - Microbiology 2023Quote: ... rodentium antigens were produced by homogenization of cultured bacterial pellet in lysis buffer (CellLytic B, Sigma-Aldrich) followed by centrifugation and harvesting of the soluble protein antigens ...
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bioRxiv - Molecular Biology 2021Quote: ... sonicated and then incubated with Pol III antibodies recognizing antigen POLR3A (18) or H3K4me3 antibodies (05-1339, Millipore). Immunoprecipitated DNA was used to generate sequencing libraries using the Takara SMARTer ThruPLEX DNA-seq Kit according to the manufacture’s protocol ...
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bioRxiv - Immunology 2019Quote: ... the total soluble tachyzoite antigen (STAg) fraction was loaded into a lactose column (Sigma-Aldrich, St. Louis, MO) and equilibrated with PBS containing 0.5 M NaCl ...
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bioRxiv - Neuroscience 2020Quote: ... Antigen retrieval was performed by heating the sections in 10 mM citrate buffer (pH 6.0, Sigma-Aldrich, USA) at > 96 °C for 20 minutes ...
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bioRxiv - Immunology 2021Quote: ... mice were primed in one hind footpad with 25mg antigen emulsified in Hunters Titermax Gold adjuvant (Sigma-Aldrich). At day 10 ...
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bioRxiv - Zoology 2019Quote: ... Antigens and PBS were mixed with equal volumes of complete Freund’s adjuvant (Sigma-Aldrich, St. Louis, MO, USA) on day 0 and with equal incomplete Freund’s adjuvant (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The antigen-antibody complexes were detected using peroxidase-conjugated goat anti-human or goat anti-mouse IgG (Sigma) and revealed using the enhanced chemiluminescence (ECL ...
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bioRxiv - Microbiology 2021Quote: ... 500 antigen-conjugated microspheres per well were added into a MultiScreen Solvinert 96 Well Filter Plate (Merk Millipore) and incubated at room temperature with plasma samples diluted in a 1:100 ratio ...
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bioRxiv - Cell Biology 2022Quote: ... Antigen retrieval was performed for 30 min in citrate buffer (Sigma-Aldrich, St. Louis, MO, C9999, 1:10). Sections were incubated with 3% hydrogen peroxidase (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... Samples were first fixed in methanol in −20°C then treated with Antigen Retrieval Buffer (pH 6, Sigma). 0.3% Triton X-100 (Sigma ...
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bioRxiv - Immunology 2023Quote: ... tetanus antigen samples were prepared by desorption with buffered Ethylenediaminetetraacetic acid (EDTA) (Sigma-Aldrich Chemie GmbH Taufkirchen Germany). Diphtheria antigen samples were prepared by desorption with sodium citrate (Sigma-Aldrich Chemie GmbH Taufkirchen ...
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bioRxiv - Developmental Biology 2023Quote: ... Antigen retrieval was conducted using a citrate buffer (created in house from Citric Acid (Sigma-Aldrich, C-0759) and Sodium Citrate (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... Sections prepared for Alexa Fluor™ antibodies were heated in 1x Citrate Antigen retrieval (Cat #c9999, Millipore Sigma) before blocking (5% Donkey serum cat#NC1697010 ...
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bioRxiv - Cell Biology 2024Quote: ... cover slips were incubated with the primary antibody (SV40 T antigen mouse-anti-human, Merck-Millipore, 1:50) overnight at 4 °C in a humidifier chamber ...
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bioRxiv - Neuroscience 2024Quote: ... the retina was first treated with pre-heated (about 70 °C) 1× antigen retrieval citrate buffer (Sigma, C9999), then followed the same protocol as with the brain section described above ...
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bioRxiv - Molecular Biology 2021Quote: ... The antigen-antibody complexes were visualized using a secondary horseradish peroxidase-conjugated goat anti-mouse IgM antibody (Sigma-Aldrich) at a dilution of 1:10,000 ...
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bioRxiv - Developmental Biology 2021Quote: ... For staining on paraffin sections antigen retrieval was performed by either 1mg/ml trypsin (Sigma, T7168, St. Louis, MO) or 10 μg/ml Proteinase K for 20 minutes at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Antigen retrieval was performed by treatment of tissue sections with porcine trypsin tablets (Cat No. T7168-20TAB, Sigma-Aldrich) for 10 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Labeled antigens were sterilized by filtering through 0.22 µm pore size Spin-X centrifuge tube filters (CLS8160, Millipore Sigma) and stored at 4°C until use ...
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bioRxiv - Immunology 2019Quote: ... scFv secretion levels were analyzed by ELISA after coating with HEL antigen (Sigma-Aldrich, 62971, 4 μg ml-1) in PBS (Thermo ...
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bioRxiv - Cancer Biology 2021Quote: ... Tumor proliferation in the R26-GB2/MMTV-PyMT animals was assessed by PCNA (proliferating cell nuclear antigen) immunostaining using the MAB424R antibody (1:10,000; clone p10, Millipore). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... was used to detect the different antigens with anti-rabbit secondary antibodies conjugated to horseradish peroxidase (1:25,000; catalog # A0545, Sigma). Either photographic film or a ChemiDoc Imaging System (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... Tissue sections were deparaffinized and antigen retrieval was achieved by use of heat-induced epitope retrieval with pH 6.0 citrate buffer (Sigma). Tissue sections were stained with H&E ...
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bioRxiv - Immunology 2020Quote: ... were cultured in 24 well plates and stimulated with inactivated SARS-COV-2 antigen (1.2 μg/ml) or PMA (25 ng/ml, cat # P8139; Sigma) and Ionomycin (1 μg/ml ...
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bioRxiv - Immunology 2020Quote: ... total IgG against these antigens were detected using HRP-conjugated respective anti-mouse antibody (Sigma-Aldrich, eBioscience and Invitrogen) and the reaction was detected using TMB substrate (BD biosciences) ...