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Citations for Millipore Sigma :
301 - 350 of 10000+ citations for Toscana Virus Glycoprotein 2 Gc Human Heterodimeric Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Wild type and 2xSE variant of MpRBOHBN with an N-terminal 6 × His tag were expressed in Escherichia coli Rosetta-gami 2(DE3) (Novagen). Cells were grown at 37°C in lysogeny broth (LB ...
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bioRxiv - Microbiology 2019Quote: ... washed cells were resuspended in incubation buffer (IB; Hank’s balanced salt solution, 2% dialysed FCS, 1x RPMI Amino Acids Solution (Sigma), 2 mM L-glutamine ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in medium containing 2% FCS with or without the PAR2 negative allosteric modulator (AZ3451, Sigma Aldrich) or a NF-κβ inhibitor (Bay11-0782 ...
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bioRxiv - Microbiology 2020Quote: ... FMC Biopolymer (90) in DMEM (containing 2% FCS and antibiotics) and serial dilutions of 5-FU (F6627, Sigma-Aldrich) or Ribavirin (R9644 ...
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bioRxiv - Microbiology 2022Quote: ... with the following modifications: Mouse anti-GST tag or mouse anti-His tag 1:200× (Sigma-Aldrich) and HRP-conjugated goat anti-mouse IgG 1:2,000× (Seracare Life Sciences ...
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bioRxiv - Cancer Biology 2021Quote: ... To transduce cells with virus cells were treated with both virus (MOI 0.5-1.0) and 5 μg / mL Polybrene infection reaction (EMD Millipore, TR-1003). For pLKO.1 vectors ...
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bioRxiv - Immunology 2021Quote: ... cells were resuspended in PBS with 2% Human Heat Inactivated AB Serum (Sigma) and 0.1 M EDTA pH 8.0 (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... Bacterial cells (2 × 106 CFU) were resuspended in nonspecific human IgG (Sigma, UK) for one hour on ice ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended in PBS with 2% Human Heat Inactivated AB Serum (Sigma) and 0.1M EDTA pH 8.0 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... and human recombinant FGF-2 (10 ng/ml; Merck Millipore, Burlington, MA, USA) as mitogens ...
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bioRxiv - Microbiology 2023Quote: ... 2 μm latex green beads were incubated with human IgM (I-8260, Sigma), then in rabbit anti-human IgM (Sigma 270A ...
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bioRxiv - Biophysics 2023Quote: BCL-2 active humans (recombinant, expressed in E. coli) were purchased from Sigma. Navitoclax ...
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bioRxiv - Physiology 2022Quote: ... 150 μl of 0.75mg/ml myelin oligodendrocyte glycoprotein 33 to 55 peptides (MOG35–55) mixed with Complete Freund’s Adjuvant (FS8810; Sigma-Aldrich) was injected subcutaneously into the tail base of 7 to 11-week-old female C57Bl/6 mice ...
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bioRxiv - Molecular Biology 2021Quote: ... a sample containing 10 μg of the spike glycoprotein was buffer exchanged with 50 mM ammonium citrate buffer (pH 6.5) using a 50-kDa MWCO filter (Millipore). The resulting buffer-exchanged sample was alkylated with a 20-fold molar excess of 4-vinylpyridine in the dark for one hour at room temperature to cap free cysteine residues ...
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bioRxiv - Neuroscience 2022Quote: ... Mice were immunized with 200 µg of myelin oligodendrocyte glycoprotein 35-55 (MOG35–55; MEVGWYRPFSRVVHLYRNGK) in incomplete Freund’s adjuvant (IFA; Sigma) supplemented with 8 mg/ml Mycobacterium tuberculosis H37Ra (Fisher) ...
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bioRxiv - Neuroscience 2023Quote: ... Mice were immunized with 200 µg of myelin oligodendrocyte glycoprotein 35-55 (MOG35–55; MEVGWYRPFSRVVHLYRNGK) in incomplete Freund’s adjuvant (IFA; Sigma) supplemented with 8 mg/mL Mycobacterium tuberculosis H37Ra (Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... bound ACE2-mouse Fc was detected by HRP-conjugated anti mouse IgG (Fc specific) (Sigma-Aldrich). Another way to do hACE2 competition ELISA is to incubate the fixed concentration of biotinylated hACE2-Fc with gradient concentrations of antibody ...
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bioRxiv - Microbiology 2022Quote: ... and then split into technical triplicates of 200 μL that were transferred and sealed into sterilized 2 mL GC headspace vials with Supelco® PTFE/silicone septum magnetic screw caps (Sigma-Aldrich®, St. Louis, MO). Samples were randomized for analysis ...
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bioRxiv - Biophysics 2019Quote: Human Gai1 subunit with an amino-terminal 8x histidine tag followed by a rhinovirus 3C protease side were expressed in Rosetta 2 (DE3) cells (EMD Millipore) using pET21a ...
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bioRxiv - Microbiology 2022Quote: ... Cleavage of the tag was performed in 2-3 ml with 20 μl 3C protease (1 mg/ml, Sigma-Aldrich, SAE0045) added and incubated over-night at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... substrates containing 400 nm diameter pores at a density of 2·106 pores·cm-2 were coated with 100 µL of 1 µg/mL human plasma fibronectin (Sigma-Aldrich) in PBS (Gibco ...
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bioRxiv - Molecular Biology 2024Quote: ... and human epithelial type 2 (HEp-2, CCL-23, ATCC) cells were cultured in Dulbecco’s modified eagle medium (DMEM, Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... For each 10 cm dish, 1 ml virus inoculum was prepared in virus growth media (VGM; containing DMEM, 0.2% bovine serum albumin (Sigma; Cat no. #A8412), 25 mM N-(2-hydroxyethyl ...
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bioRxiv - Immunology 2019Quote: ... Spleens and lymph nodes were cut into small pieces and digested in IMDM (2% FCS) containing collagenase type IA (1 mg/ml, Sigma) and DNase I (10 U/ml ...
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bioRxiv - Biophysics 2019Quote: ... fab-fragment solution consisting of 100 μg/ml F(ab’)2 fragments (anti-mouse IgG [Fc specific] antibody developed in goat; Sigma) in PBS that bind unspecifically to the hydrophobic surface (incubation time 5 min) ...
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bioRxiv - Biophysics 2020Quote: ... the cells were incubated with a FCS-free media supplemented with 2 nM brain-derived neurotrophic factor (BDNF) (Merck Millipore). After 2 days ...
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bioRxiv - Cell Biology 2021Quote: ... then added to cells and incubated for 2 hours before discarding and adding a 2.4% (w/v) microcrystalline cellulose and 2% FCS (mixed 1:1) overlay (Sigma-Aldrich). The assay was incubated at 37°C and 5% CO2 for 70 hours then fixed in 4% PFA for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... flow channels of the Drop-seq chip were subjected to a hydrophobicity treatment using 1H,1H,2H,2H-Perfluorodecyltrichlorosilane (in 2% v/v in FC-40 oil; Alfa Aeser/Sigma). After 5 min of treatment ...
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bioRxiv - Immunology 2019Quote: The large intestine was opened longitudinally with blunt ended scissors and cut into 0.5cm segments before washing in 2% foetal calf serum (FCS) in Hank’s Balanced Salt Solution (HBSS) (Sigma, Life Sciences). To remove epithelial cells ...
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bioRxiv - Cancer Biology 2019Quote: ... and resuspended in 50 to 80 µl FC buffer containing 0.1 µg/ml 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich). Primary tumor samples were stained with CD45-APC-Cy7 (Biolegend ...
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bioRxiv - Immunology 2023Quote: Cells were cultured in R10 medium, (RPMI 1640 containing 10% heat inactivated FCS, 50μM 2-mercaptoethanol with penicillin and streptomycin, (Sigma-Aldrich) and incubated at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Pellets were recovered in PBS 2% FCS and erythrocytes were removed using a red blood cell lysis buffer (Sigma-Aldrich) before being filtered onto a 40-µm cell strainer (Dutscher) ...
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bioRxiv - Biophysics 2022Quote: ... DMSO (dimethyl sulfoxide, BioUltra 99.5% GC-41639, Sigma-Merck, KGaA, Darmstadt, Germany) and ethanol (puriss ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were reconstituted in 100 μL hexane (GC grade, Sigma Aldrich, USA). GC-MS analysis was performed using an Agilent 8860 GC system equipped with an Agilent 7683 N auto sampler and a Agilent J & W GC columns (30 m × 0.25 mm × 0.2 μm ...
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bioRxiv - Genomics 2023Quote: The lentiviral titer was determined by transducing 400,000 cells with increasing volumes (0, 1, 2, 5, 10, 20, and 40 μl) of concentrated virus and polybrene (6 μg/ml; Millipore, TR-1003-G). 48 hours after the transduction ...
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bioRxiv - Molecular Biology 2020Quote: ... HA tag antibody (HA-7, Sigma-Aldrich), Myc tag antibody (A7 ...
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bioRxiv - Microbiology 2019Quote: ... and the FLAG epitope tag (F1804; Sigma), as well as anti-mouse HRP (A9044 ...
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bioRxiv - Physiology 2019Quote: ... anti-HA tag (Cat. No# H6908, Sigma), anti-Rabbit secondary (Cat ...
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bioRxiv - Cell Biology 2020Quote: ... anti-His tag (HIS.H8, 1:1,000, Millipore). The HRP-conjugated anti-mouse (111–035-144 ...
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bioRxiv - Biochemistry 2020Quote: ... and the Flag tag (Sigma-Aldrich, F3165). ASCC2 and ASCC3 bands were quantified using Image J software ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-HA-tag (clone 3F10, Sigma, #11867432001), anti-giantin (BioLegend ...
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bioRxiv - Physiology 2021Quote: ... and the FLAG tag (F1804, Sigma-Aldrich).
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bioRxiv - Cell Biology 2021Quote: ... The antibodies against FLAG-tag (Sigma F3165), H6PDH (Abcam ab119046) ...
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Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... Flag-tag (Sigma, F3165; dilution 1:5,000); Horseradish peroxidase (HRP ...
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bioRxiv - Molecular Biology 2020Quote: ... Myc tag antibody (A7, Abcam; 4A6 Millipore).
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bioRxiv - Immunology 2020Quote: ... cOmplete His-Tag Purification Resin (Sigma Millipore) was applied for affinity purification ...
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bioRxiv - Immunology 2020Quote: ... cOmplete His-Tag Purification Resin (Sigma Millipore) was applied for affinity purification ...
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bioRxiv - Plant Biology 2022Quote: ... and the HA-tag (Sigma-Aldrich H3663). Anti-rabbit-HRP (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... Flag-tag (Sigma-Aldrich Cat# F1804, RRID:AB_262044), DHFR (Santa Cruz Biotechnology Cat# sc-377091 ...
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bioRxiv - Immunology 2019Quote: ... Flag-tag (Flag M2-Peroxidase, Sigma-Aldrich) and actin (AC15 ...