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Citations for Millipore Sigma :
101 - 150 of 10000+ citations for Toscana Virus Glycoprotein 2 Gc Human Heterodimeric Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ADCP was performed using CHO cells stably expressing full-length wild-type SARS-CoV-2 S glycoprotein (target cells) fluorescently labelled with PKH67 Fluorescent Cell Linker Kits (Sigma Aldrich; Cat. Nr.: MINI67). Target cells were incubated with titrated concentrations of mAbs for 10 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... recombinant ephrins or Fc were preclustered in a 5:1 molar ratio with either mouse or goat anti-human Fc (Millipore Sigma cat. no. 16760 and 12136 respectively) for 30’ at 37°C.
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bioRxiv - Biochemistry 2020Quote: LX-2 human hepatic stellate cells [47] (Millipore) were cultured in DMEM supplemented with 10% fetal bovine serum at 37°C under 5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... LX-2 human stellate cells (Millipore, Billerica, MA); and THP-1 human monoblast cells (EMD Millipore ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 2 % human serum (HS, Sigma-Aldrich) at a concentration of 0.5×106 cells/ml ...
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bioRxiv - Biophysics 2020Quote: ... ≥ 99.5% (GC) was purchased from Sigma Aldrich, France ...
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bioRxiv - Biochemistry 2020Quote: ... PCNA-12 co-expression plasmids were constructed by inserting non-tagged PCNA-1 into multiple cloning site 1 and PCNA-2 (without and with a His6-tag) into site 2 of pET-DUET-1 plasmid (Novagen).
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bioRxiv - Microbiology 2020Quote: ... Anti-ICP4 MAb H1A021 or monoclonal Ab to VSV glycoprotein G (P5D4, Sigma) was added for 1 h followed by Alexa Fluor 488-labeled goat anti-mouse IgG (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... Then 1 µg/well of biotinylated α1-acid glycoprotein (Sigma, Catalogue number-112150) or 3’-sialyllactose-PAA-biotinylated (Glycotech ...
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bioRxiv - Microbiology 2020Quote: ... cells were stained with 1/200 diluted Goat Anti human IgG Fc-FITC antibody (Cat: F9512, Sigma) for 45 min and analyzed using flow cytometry (CytoFLEX ...
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bioRxiv - Bioengineering 2021Quote: ... and binding was detected with an anti-human IgG (Fc specific) (1:5000 dilution, Sigma-Aldrich, A0170) or anti-FLAG (1:1000 dilution ...
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bioRxiv - Bioengineering 2023Quote: ... Antibodies and dyes used in this study were: Anti-Human IgG Fc specific (Millipore Sigma I2136-1ML), Anti-6X His tag (Abcam ab9108) ...
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bioRxiv - Biochemistry 2020Quote: ... FLAG tag (F1804) or His tag (H1029) were from Sigma. Mouse monoclonal antibody raised against hemagglutinin (HA ...
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bioRxiv - Immunology 2020Quote: ... FCS (Sigma Life Science ...
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bioRxiv - Immunology 2021Quote: ... The cultures were supplemented on day 2 with FCS (10% v/v; Sigma-Aldrich). Medium was replaced every 3 days thereafter with fresh R+ containing FCS (10% v/v ...
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bioRxiv - Cell Biology 2021Quote: ... Flag-tag (Sigma), Myc-tag (Upstate) ...
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bioRxiv - Biophysics 2022Quote: ... the N-terminal hexa-histidine tag was cleaved with ∼2 Units of bovine thrombin (Sigma) per 1 mg of protein ...
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bioRxiv - Microbiology 2022Quote: ... varicella-zoster virus (Light Diagnostics, Varicella-Zoster Virus DFA kit, EMD Millipore, Temecula, CA), adenovirus (D3Ultra Respiratory Virus Screening & Identification kit ...
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bioRxiv - Systems Biology 2022Quote: ... The virus was collected both 2 days and 3 days later and 0.45 μm syringe filters (Millipore) were used to eliminate cells ...
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bioRxiv - Microbiology 2022Quote: ... Each SARS-CoV-2 virus was isolated after three passages in Vero cells in DMEM (Sigma Aldrich). Virus isolation was confirmed by RT-PCR using E gene-specific primer sets (Extended Data Table 2) ...
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bioRxiv - Cell Biology 2023Quote: ... 2×106 cells were combined with 300 µL of virus and 0.8 µL/mL polybrene (Millipore, #TR1003G) in the well of a 12-well plate (final volume of 2 mL) ...
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bioRxiv - Genomics 2019Quote: ... 100 U/mL recombinant human IL-2 (Sigma-Aldrich) and 1% penicillin-streptomycin (final concentration 100 U penicillin ...
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bioRxiv - Genomics 2020Quote: ... 100 U/mL recombinant human IL-2 (Sigma-Aldrich) and penicillin-streptomycin (final concentration 100 U penicillin ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... 100 U/mL human interleukin-2 (Millipore Sigma; 11011456001), 2 ng/mL human interleukin-7 (Peptrotech ...
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bioRxiv - Microbiology 2022Quote: ... and human thrombin (final concentration 2 U/ml; Sigma) in 50 μl Hank’s Balanced Salt Solution supplemented with 400 μg/ml aprotinin (Sigma) ...
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bioRxiv - Immunology 2024Quote: ... and 100 U/ml human IL-2 (Sigma-Aldrich). After initial activation by plate-bound 0.05 µg of anti-CD3 (homemade ...
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bioRxiv - Bioengineering 2019Quote: ... tissue culture polystyrene culture plates were pre-coated with 20 mg/ml anti-IgG (human, Fc-specific, Sigma) for 1 hour at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... We detected bound antibody with horseradish peroxidase (HRP)-coupled anti-human (Fc) antibody (Sigma Aldrich catalog number A0170).
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bioRxiv - Microbiology 2022Quote: ... Mouse monoclonal antibody MAB8150 against WNV E glycoprotein was from EMD Millipore (Billenca MA). Alexa Fluor 488 goat anti-mouse IgG (H+L ...
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bioRxiv - Microbiology 2020Quote: ... dichloromethane extracts were concentrated in vacuo and placed in a vial with 150 µL of dichloromethane and 2 µL of 26.7 ppb of toluene (GC grade, Sigma-Aldrich) (internal standard) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The GC cell pellet was further suspended in 1-2 ml of serum free Dulbecco’s Modified Eagle Medium (DMEM) (Sigma, U.S.A) supplemented with 3 mM/ml of L-glutamine (Sigma ...
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bioRxiv - Zoology 2019Quote: ... (±)-geosmin (Sigma-Adrich, Cat. Nr. UC18, ≥98% (GC)) ...
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bioRxiv - Neuroscience 2023Quote: ... Nonanol (1-nonanol, Sigma Aldrich, purum, ≥ 98.0% (GC) - concentration ...
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bioRxiv - Microbiology 2019Quote: ... 1.8 μg of pRSV-Rev and 1.5 μg of plasmid containing pAIP-NSP2-mCherry or pAIP-NSP5 and the human immunodeficiency virus long terminal repeats were co-transfected with Lipofectamine 3000 (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... Cells were washed with FACS buffer (PBS, 2%FCS) and fixed in 4% Paraformaldehyde (Sigma) in PBS for 10 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... and re-suspended in ice cold 2 % (v/v) FCS/PBS (PAA Laboratories/Sigma Aldrich) following centrifugation ...
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended in flow cytometry buffer (FB: 2% FCS, 3 nM EDTA (Sigma, E9884) in PBS) ...
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bioRxiv - Cell Biology 2021Quote: ... the virus-containing medium was removed and replaced with selection medium containing 2 μg/ml Puromycin (Sigma–Aldrich). After 7 days of selection ...
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bioRxiv - Microbiology 2022Quote: ... Thiol concentration at the same intervals as cells infected with virus was determined by Ellman’s reagent (5,5’-dithio-bis-2-nitrobenzoic acid, DTNB, Sigma) by reading the optical density at 412 nm in a plate reader (Biotek Cytation 5 ...
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bioRxiv - Microbiology 2021Quote: ... E2 (CHIK-102 and CHIK-263) [42] or Zika Virus E as a control (ZV-2, Sigma-Aldrich) (kindly provided by Dr ...
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bioRxiv - Cell Biology 2023Quote: ... the virus-containing media was removed and N2 media with doxycycline (DOX, 2 µg/mL, Sigma, catalog #: D9891) was added ...
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bioRxiv - Microbiology 2023Quote: ... Virus-plasma mixture were aspirated and 2ml of 1% methylcellulose (combination of 2% methylcellulose (Sigma, Cat#435244-250G) and equal parts of 2X MEM (Gibco ...
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bioRxiv - Genetics 2019Quote: ... 10% FCS (Sigma) supplemented with FLT3L (50 ng/ml) ...
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bioRxiv - Cell Biology 2021Quote: ... 10 % FCS (Sigma) and P/S (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... + 10% FCS (Sigma,) + 1 x (final concentration ...
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bioRxiv - Cell Biology 2021Quote: ... 10% FCS (Sigma), 2 mM L-glutamine ...
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bioRxiv - Biochemistry 2023Quote: ... 5 % FCS (Sigma) in DPBS for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... 10% FCS (Sigma), NEAA ...
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bioRxiv - Cell Biology 2021Quote: ... FLAG-tag (Sigma F3165), Actin (Sigma A2066) ...
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bioRxiv - Cell Biology 2021Quote: ... Flag-tag (M2, Sigma), TOP2A (M042-3 ...