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Citations for Millipore Sigma :
1 - 50 of 10000+ citations for Tick Borne Encephalitis Virus Lysate Strain Moscow B 4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: Anti-Japanese encephalitis E (mouse monoclonal, Millipore), influenza A H9N2 HA (mouse monoclonal ...
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bioRxiv - Immunology 2024Quote: ... coli strain B (Sigma, D4889). To knockdown NFKB1 and p65 ...
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bioRxiv - Microbiology 2023Quote: ... coli strain B-Type VIII (Sigma-Aldrich), was added at 0.25 μg/ml ...
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bioRxiv - Plant Biology 2023Quote: ... or Rosetta-gami B strains (all from Merck Millipore) and purified using Pierce Glutathione Magnetic Beads (Thermo Scientific) ...
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bioRxiv - Immunology 2022Quote: ... 1 μL of virus lysate was treated with 250 U of benzonase nuclease (Sigma) at 37°C for 1 h ...
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bioRxiv - Immunology 2022Quote: Protein lysate was extracted using CelLytic B Cell Lysis Reagent (Sigma) following the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2019Quote: ... containing 4 μg/mL hygromycin B (Millipore Sigma) at 37°C in a 5% CO2/95% air atmosphere and passaged every 3-4 days ...
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bioRxiv - Pathology 2021Quote: ... and 4% dodecyl- b-D-maltoside (Sigma-Aldrich), were separated by Blue Native-PAGE Novex 4– 16% Bis-Tris gel (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2019Quote: ... containing 4 μg/mL hygromycin B (Millipore Sigma) at 37°C in a 5% CO2/95% air atmosphere and passaged every 3-4 days ...
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bioRxiv - Biochemistry 2023Quote: ... of p150 from rubella virus strain RVi/Brooklyn.NY.USA/98/1B CRS (GenBank JN635282; UniProt G3M8F4) was cloned into the pET24a plasmid (Novagen) with a C-terminal hexahistidine purification (His6 ...
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bioRxiv - Plant Biology 2021Quote: ... a 4-methylumbelliferyl b-D-glucuronide (MUG) (Sigma-Aldrich) assay was carried out as previously described (He et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 mL b-mercaptoethanol (Sigma M6250; stock 14.3 M), 50 mL LIF (Chemico ...
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bioRxiv - Genomics 2021Quote: ... 15 g/L agar) supplemented for mutant strains with hygromycin B (Sigma-Aldrich; 70 μg/ml) or nourseothricin (Werner Bioagents ...
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bioRxiv - Immunology 2022Quote: ... Strains expressing GFP were grown in media supplemented with 50 μg/mL hygromycin B (Sigma-Aldrich), and strains expressing luxABCDE were grown in media supplemented with 20 μg/mL Zeocin (Thermo).
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were mixed with 4 × Laemmli sample buffer (Sigma) and boiled for 10 min at 95°C ...
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bioRxiv - Molecular Biology 2021Quote: ... pombe (strain SHY1058) cultures were labeled with 5 mM 4-thiouracil (4-thioU) (Sigma-Aldrich #440736) for 4 min ...
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Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: ... Virus was then concentrated using Amicon Ultra-4 centrifugal filter units (Merck-Millipore) and the solution was filtered with a 0.2 µm Acrodisc syringe filter (Sigma-Aldrich).
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bioRxiv - Microbiology 2021Quote: ... Influenza viruses as well as vesicular stomatitis virus strain Indiana (VSV) were propagated on Madin-Darby canine kidney (MDCKII) cells cultured in minimal essential medium (MEM-Sigma) containing 10% (v/v ...
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bioRxiv - Bioengineering 2020Quote: ... virus was collected after 120 hours from both cell lysates and media and purified over iodixanol (Optiprep, Sigma).
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bioRxiv - Immunology 2022Quote: ... scapularis ticks were surface sterilized with continuous agitation in 10% benzalkonium chloride (Sigma, 12060) for 10 minutes ...
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bioRxiv - Microbiology 2021Quote: ... treated with 4 μM cheopin or 10% CelLytic B (Sigma), and intracellular accumulation of dye over time was measured by quantifying fluorescence intensity (490 nm ex ...
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bioRxiv - Microbiology 2019Quote: ... For each strain 60 µl lysate were incubated with 6 µl amyloglucosidase (200 U/ml, Sigma-Aldrich) (quantification of glucose stored as glycogen and free glucose ...
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bioRxiv - Microbiology 2021Quote: ... Virus were then buffer exchanged with Amicon Ultra-4 Centrifugal Unit (Millipore Sigma, #UFC800396) to get rid of furin inhibitor in the media.
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bioRxiv - Microbiology 2022Quote: ... and 1:4 800 000 in virus growth medium (VGM) containing MEM (Sigma, 2279), 2 % FBS ...
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bioRxiv - Microbiology 2021Quote: ... freeze-thaw cycles) was followed by concentration of virus suspensions (i.e., filtered lysates) using 30kDA MWCO spin concentrator (Sigma-Millipore). From the retentate ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908, Sigma, 1:1,000). The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012 ...
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bioRxiv - Genetics 2022Quote: Whole ticks or dissected organs were homogenized in TRI Reagent (Sigma Aldrich, Saint Louis, MO) in a 1.5 mL microcentrifuge tube with a pestle (Kimble Kontes ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysates are obtained by sonication and CelLytic™ B Cell Lysis Reagent (Sigma-Aldrich, US). Cell debris are removed by centrifugation at 24,000 g and crude Rubiscos were obtained by ammonium sulfate precipitation ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were reduced by the addition of 4 mM TCEP (Sigma) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: Lysates were reduced by the addition of 4 mM TCEP (Sigma) for 30 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... a sandwich ELISA was used in which 1% Trixon X-100-treated virus lysates were added to plates pre-coated with ConA (50 µg/ml, Sigma) and reacted with anti-Env mAbs 66 ...
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bioRxiv - Microbiology 2021Quote: ... freeze-thaw cycles) was followed by concentration of virus suspensions (i.e., filtered lysates) using 30kDA MWCO spin concentrator (Sigma-Millipore). From the retentate ...
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bioRxiv - Molecular Biology 2021Quote: ... Primer extension was conducted with 4 units of Avian Myeloblastosis Virus (AMV) reverse transcriptase (Sigma) for 1 hour at 15°C ...
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bioRxiv - Systems Biology 2020Quote: Recombinant HA-tagged Gln4 protein for use as a standard was produced by transforming E.coli strain Rosetta-gami B (DE3; Novagen) with pET19b-GLN4 ...
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bioRxiv - Microbiology 2024Quote: ... The presence or absence of bacteria in infected and uninfected samples was checked through staining with Phenolic Auramine O-Rhodamine B dye (4) (1/3 dilution of stock solution) (Auramine O-861020-25gm, Sigma) (Rhodamine B-R6626-100gm, Sigma). Coverslips were mounted on glass slides and visualized using CLSM with a 488 nm laser.
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bioRxiv - Cell Biology 2021Quote: ... Lysates were vortexed 4 x 45 sec with glass beads (Sigma Aldrich), maintained on ice between pulses ...
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bioRxiv - Immunology 2022Quote: ... Virus was concentrated from media using PEG Virus Precipitation Kit (Sigma). Viral titer was determined by QuickTiter™ Lentivirus Associated HIV p24 Titer Kit (Cell Biolabs ...
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bioRxiv - Plant Biology 2019Quote: ... Pseudomonas syringae strains were grown at 28°C (with shaking at 200 rpm) in King’s B liquid medium (Sigma-Aldrich) containing appropriate antibiotics (King et al. ...
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bioRxiv - Cell Biology 2023Quote: Rho family GTPase proteins were expressed using a modified pGEX vector transformed into Rosetta-Gami B BL21(DE3) pLysS strains (Novagen). Protein was purified from these cells by freeze-thaw lysis in Hepes buffer (20mM Hepes ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysate (1 mg) from various yeast strains was used in immunoprecipitation with anti-Flag M2 magnetic beads (20 μl, Sigma) in a total volume of 1.5 ml of IP-Lysis buffer and incubated with end-over-end rotation for 4 h at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012, Sigma, 1:1,000). Loading control for cell lysate was detected with anti-α-tubulin (rabbit polyclonal ...
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bioRxiv - Immunology 2023Quote: ... melanoma was induced by injecting B6.Cg-Tg(Tyr-cre/ERT2)13Bos BRAFtm1Mmcm Ptentm1Hwu/BosJ (BRAFV600E PTEN-/- Tyr::CreERT2, Strain #013590) mice subdermally with 4-hydroxytamoxifen (4-HT)(Millipore Sigma, H6278 ...
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bioRxiv - Microbiology 2019Quote: ... and 4 mg polymyxin B (EMD Millipore Corp., USA, cat. no. 5291-500MG) in a mixture of 1 ml of Mueller-Hinton broth (BD Diagnostics Systems ...
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bioRxiv - Microbiology 2021Quote: ... Dilutions were plated on 1/4-diluted King’s B+++ Agar plates (Sigma-Aldrich) supplemented with 13 mg/L chloramphenicol ...
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bioRxiv - Bioengineering 2022Quote: ... lungs were rinsed with antibiotics/antimycotics (10% P/S, 4% amphotericin-B [Sigma] ...
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bioRxiv - Bioengineering 2021Quote: ... cells were transduced with 4 µL library virus/well along with 8 µg/mL Polybrene (Sigma-Aldrich) aiming for an MOI at 0.3-0.4 (Supplementary Methods and Results) ...
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bioRxiv - Immunology 2022Quote: ... Cells were then cultivated with the virus in the presence of 4 μg/ml polybrene (Sigma-Aldrich) for 12 hr.
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bioRxiv - Microbiology 2022Quote: ... virus was preincubated for 1 hr at 4 °C with 100 μg/mL soluble H (Sigma H4784), HS (Sigma H7640) ...
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bioRxiv - Biochemistry 2019Quote: EcSerRS (from E. coli strain B ER2560) and SaSerRS (from S. aureus seg50 (1150)) were cloned into pET52b(+) vector (Merck Millipore, Germany) using the NcoI and SacI restriction sites allowing for the production of protein with a thrombin cleavable C-terminal His10-tag ...
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bioRxiv - Immunology 2021Quote: ... The Mb3601-EGFP fluorescent strain were derived by electroporation with an integrative plasmid expressing EGFP and selected with Hygromycin B (50 μg/mL) (Sigma, USA) as described in previously [33] ...