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Citations for Millipore Sigma :
251 - 300 of 10000+ citations for Tick Borne Encephalitis Virus Lysate Strain Moscow B 4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Aggregated virus was removed by a 0.22µm filter (Millipore). Sub-confluent monolayer of MEFs cultured in DMEM ...
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bioRxiv - Physiology 2020Quote: ... Virus was filtered with a 0.45mm OVDF filter (Millipore). Supernatants were collected and purified using PEG-it (System Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... supernatant containing virus was filtered with 0.22μm filters (Millipore) and store at −80◻ in aliquots.
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bioRxiv - Cancer Biology 2023Quote: ... adherent cells were infected with COL7A1 virus (Sigma Aldrich) (1–5 MOI (multiplicity of infection) ...
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bioRxiv - Molecular Biology 2023Quote: ... and the virus was concentrated using polyethylglycol (Sigma, P4338). Cells were infected in medium containing 5 μg ml−1 polybrene (Millipore ...
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bioRxiv - Molecular Biology 2019Quote: ... 4 mg of lysate proteins were incubated with 40 μl of pre-washed anti-FLAG M2 magnetic beads (Sigma– Aldrich) for 2 h ...
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bioRxiv - Neuroscience 2019Quote: ... lysates of tissues or tells were incubated with antibodies overnight (4°C) and subsequently with protein G-agarose beads (Millipore) for 5h (4 °C) ...
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bioRxiv - Cell Biology 2020Quote: ... protein lysate was immunoprecipitated for 2 hours at 4°C with the following antibodies: rabbit anti-myc (2.5 μg, Millipore Sigma), mouse anti-HA (2.5 μg ...
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bioRxiv - Microbiology 2022Quote: ... Equal amounts (in micrograms) of lysates were separated on 4-15% SDS-PAGE gel and transferred to Polyvinylidene difluoride (PVDF) membrane (Millipore). Subsequently ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lysates were centrifuged at 20,000 × g for 20 min at 4°C and the total protein lysate was quantified using Bradford Reagent (B6916; Sigma). Then ...
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bioRxiv - Pathology 2020Quote: ... IP was performed by incubation of the resulting lysates overnight at 4 °C with mouse monoclonal anti-Flag antibody (Cat.# F3165, Sigma) or with normal mouse IgG (Cat.#A7028 ...
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bioRxiv - Biochemistry 2021Quote: ... The second half of the clarified lysate was incubated for 2 h at 4°C with 20 μl of Anti-FLAG M2 magnetic beads (Sigma) equilibrated in FLAG wash buffer containing 20 mM HEPES pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... The lysates were then incubated for 60 min at 4 °C with anti-FLAG M2 Affinity Gel (A2220, Sigma-Aldrich). The beads were washed three times with immunoprecipitation wash buffer [50 mM HEPES-NaOH (pH 7.2) ...
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bioRxiv - Cell Biology 2022Quote: ... cell lysates were incubated in a rotating incubator for 1 h at 4°C with bead-conjugated FLAG (Sigma; A2220), bead-conjugated HA (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: 400 μl of cell lysate was incubated overnight at 4 °C with 2 μg of anti-Myc or anti-Flag antibody (Sigma). Protein A-Sepharose (GE ...
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bioRxiv - Microbiology 2023Quote: ... and remaining lysate was end-over-end incubated with 5 µg of primary Ab overnight at 4°C [Rabbit-anti-HA (Sigma), Rabbit-anti-Flag (Sigma) ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein lysates were denatured at 95°C for 5mins before being loaded into a TruPAGE 4-20% precast gradient gel (Sigma) and undergoing SDS-PAGE at 120 V for 2 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were spun at 30,000 g for 10 min at 4°C and the cleared lysate was incubated with 40 µl anti-FLAG agarose bead slurry (Sigma) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Two mL of lysate were incubated overnight at 4°C with 20 μl of Anti-Flag M2 magnetic beads (Sigma) or Anti-RPA32 conjugated Dynabeads (2μg of MABE285 anti-RPA34-20 mouse (Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... coli strain BL21 Rosetta 2 (DE3) (Novagen) and cultured in 2×YT medium at 18°C for 16 h following induction with 0.2 mM IPTG ...
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bioRxiv - Biochemistry 2020Quote: ... coli strain BL21 (DE3) competent cells (Novagen) that were grown on agar plates containing 50 μg/ml kanamycin ...
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bioRxiv - Microbiology 2019Quote: ... pneumophila strains were cultured in ACES (Sigma)-buffered yeast extract broth (pH = 6.9 ...
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bioRxiv - Biochemistry 2020Quote: ... coli BL21 (DE3) Codon-Plus strain (Novagen). When the culture of the transformed cells reached an OD600 of 0.5 ...
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bioRxiv - Biophysics 2021Quote: ... coli host strain BL21(DE3)-Rosetta2 (Novagen). Cells were grown at 37 °C in TB medium ...
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bioRxiv - Plant Biology 2021Quote: ... coli strain Rosetta 2 (DE3) pLysS (Novagen) and purified using Ni-NTA resin (Qiagen ...
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bioRxiv - Biochemistry 2020Quote: ... coli strain Rosetta 2(DE3) pLysS (Novagen), and grown in the presence of ampicillin or kanamycin and chloramphenical to an OD600 of 0.8 at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... coli strain Rosetta 2 (DE3)(Novagen Inc.).
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bioRxiv - Immunology 2021Quote: ... coli strain Rosetta 2(DE3) pLysS (Novagen), and grown in the presence of ampicillin and chloramphenicol to an OD600 of 0.8 at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... coli Rosseta 2 (DE3)/pLysS strain (Novagen) was transformed with pSK51 ...
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bioRxiv - Plant Biology 2020Quote: ... coli strain BL21 Star (DE3, Sigma-Aldrich) for recombinant protein expression ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli strain BL21 Star™ (DE3) (Novagen) was used for expression and purification of sfGFP containing a C-terminal glycosylation tag34 and polyhistidine tag (sfGFPDQNAT-6xHis) ...
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bioRxiv - Cancer Biology 2020Quote: ... coli BL21 (DE3) Codon-Plus strain (Novagen) transformed with the reconstructed expression plasmid was cultured at 37 °C in LB medium containing 0.05 mg/ml ampicillin until OD600 of medium reached 0.8 ...
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bioRxiv - Bioengineering 2022Quote: ... coli strain Origami 2 DE3 (Novagen, UK) was co-transformed by electroporation with pBirAcm and either pMAL-c5x-NtGnTI-GS-AviTag or pMAL-c5x-hGalT-GS-AviTag ...
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bioRxiv - Genetics 2022Quote: ... coli BL21 (DE3) expression host strain (Novagen) was transformed with the pDEST14-PSO2 expression vector ...
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bioRxiv - Cell Biology 2022Quote: ... coli cells (BL21 Rosetta-gami strain; Novagen) using 0.2 mM isopropyl_β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Microbiology 2021Quote: ... coli Rosseta 2 (DE3)/pLysS strain (Novagen) was transformed with pPH158 (33) ...
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bioRxiv - Microbiology 2021Quote: ... Strains were streaked onto LB agar (Sigma) and incubated overnight at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... coli strain Rosetta 2(DE3) pLysS (Novagen), and grown in the presence of ampicillin and chloramphenical to an OD600 of 0.8 at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... strain BL21(DE3) (Novagen, Madison, Wis., USA), and purification of protein was carried out as described previously [27] ...
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bioRxiv - Microbiology 2023Quote: ... coli strain Rosetta 2 (DE3) cells (Novagen) transformed with pET-Cas9-NLS-6xHis (Addgene plasmid #62933 ...
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bioRxiv - Bioengineering 2023Quote: ... Escherichia coli strain BL21 (DE3) cells (Novagen) were transformed using the expression vector and grown at 37°C in a 2× YT broth ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... coli strain Rosetta2(DE3)pLysS (EMD Millipore) was transformed with the pET-based Smt3-Cereblon-TBD expression plasmid ...
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bioRxiv - Biochemistry 2023Quote: ... a chemically competent strain (Sigma-Aldrich, Germany). For purification ...
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bioRxiv - Plant Biology 2024Quote: ... coli strain Rossetta2(DE3)pLysS (Novagen/Merck).
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bioRxiv - Evolutionary Biology 2021Quote: ... 2019) or the background strain CEA17) were incubated 4 h at 37°C in Czapek-Dox medium (CZD, Sigma-Aldrich Chemie, Munich, Germany) to induce swelling ...
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bioRxiv - Cell Biology 2023Quote: ... total RNA was extracted from groups of 4-5 aphids of the above-described strains using TRI Reagent® (T9424, Sigma-Aldrich, USA) and Direct-Zol RNA extraction Kit (R2052 ...
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bioRxiv - Biochemistry 2022Quote: ... FSL-B(tri) (Function-Spacer-Lipid with blood group B trisaccharide; Sigma Aldrich) or lactosylceramide (LC ...
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bioRxiv - Immunology 2023Quote: ... GC-B cells and follicle B cells were stained with Pna-HRP (Sigma) and anti-mouse IgD-BIOT (SouthernBiotech ...
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bioRxiv - Biophysics 2019Quote: ... and the pellet was resuspended in 4 mL/g-pellet of B-PER (ThermoScientific, cat# 78266) with 1 mM PMSF (Millipore Sigma, cat# 7110 ...
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bioRxiv - Synthetic Biology 2019Quote: ... Sigma-Aldrich and 50 µg mL−1 of 5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside (X-Gal) (no. B4252; Sigma-Aldrich).