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Citations for Millipore Sigma :
351 - 400 of 1090 citations for Solid Phase Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... RNA extraction was carried out using TRI-reagent (Sigma Aldrich) following the supplier’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... and lysed by 20 mL Bugbuster protein extraction buffer (Millipore, 50 mL Bugbuster lysis buffer contains - 49 mL Bugbuster reagent ...
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bioRxiv - Physiology 2022Quote: ... extracted using the GenElute™ Gel Extraction Kit (SIGMA NA1111) and purified with the GenElute™ PCR Clean-Up Kit (SIGMA NA1020) ...
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bioRxiv - Molecular Biology 2023Quote: ... two extractions with 25:24:1 phenol:chloroform:isoamyl alcohol (Sigma-Aldrich), and one extraction with chloroform (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2022Quote: RNA extraction was carried out using Tri Reagent (Sigma-Aldrich). Reverse transcription (RT ...
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bioRxiv - Microbiology 2023Quote: ... using the GenElute Bacterial Genomic DNA extraction kit (Sigma Aldrich), using the standard protocol with 200 μL phage lysate taken directly from the bioscreen plates ...
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bioRxiv - Cell Biology 2024Quote: ... Genomic DNA was extracted using extraction solution (E7526; Millipore Sigma), tissue preparation solution (T3073 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and a CelLytic™ NuCLEAR™ Extraction Kit (Sigma-Aldrich), respectively ...
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bioRxiv - Neuroscience 2023Quote: CelLytic™ NuCLEAR™ Extraction Kit (Millipore Sigma, NXTRACT-1KT) was used to perform cell fractionation following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... 2015 using a compartment protein extraction kit (Millipore cat: 2145). Triturated brain tissue was thawed on ice for ∼30 minutes before being homogenized in 2 ml/g of Buffer C (HEPES (pH7.9) ...
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bioRxiv - Biochemistry 2024Quote: ... The extraction buffer composition was: 30 mM HEPES (Sigma-Aldrich) pH 7.4 ...
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bioRxiv - Immunology 2024Quote: ... and then separated by liquid-liquid extraction using hexane (Sigma). The hexane extract was then dried under a fume of nitrogen at 45 C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The diluted antigen (100μl) was coated to the wells of a Nunc-Immuno™ MicroWell™ 96 well solid plates (Sigma-Aldrich, MO, USA) and was incubated overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... the supernatant was discarded and aliquots (20 μL) of the remaining solution (500 μL) were plated on solid Reasoner’s 2A (R2A; Sigma-Aldrich, Merck, Rahway, NJ, USA) to confirm the absence of bacterial growth seven days after incubation at 25°C.
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bioRxiv - Synthetic Biology 2021Quote: ... The OA phase (pH = 7.8) also contained 50mg/mL of Kolliphor P-188 (Sigma-Aldrich, UK). For each OLA experiment ...
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bioRxiv - Biophysics 2021Quote: ... cells were synchronized in late G2 phase using the cell-cycle blocker RO3306 (Sigma-Aldrich, SML0569) [15] ...
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Differential turnover of Nup188 controls its levels at centrosomes and role in centriole duplicationbioRxiv - Cell Biology 2019Quote: ... U2OS cells were arrested in S-phase by treatment with 2 μg/ml aphidicolin (Millipore Sigma) for 24 h ...
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bioRxiv - Microbiology 2019Quote: Treatments were started from log phase cultures grown overnight in the absence of cyanocobalamin (Sigma-Aldrich) and diluted in fresh media to an OD600 of 0.05 ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were first arrested in S phase by the addition of 2 mM thymidine (Sigma-Aldrich) for 19 h ...
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bioRxiv - Microbiology 2021Quote: ... The aqueous phase was then removed and added to an equal amount of 100% Ethanol (Sigma). Final RNA extraction was carried out using the ZYMO Direct-zol Mini-Prep Plus Kit (Zymo Research ...
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bioRxiv - Cell Biology 2021Quote: ... Hydrophobicity of silicon mold was maintained by gas-phase trichloro- perfluoroctylsilane deposition (Sigma-Aldritch 448931-10G) for 30 minutes in vacuum chamber followed by curing at 150 deg for 10 minutes ...
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bioRxiv - Microbiology 2021Quote: ... The aqueous phase was transferred to a new tube containing linear polyacrylamide (Gene-EluteTM LPA, Sigma). To allow RNA precipitation ...
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bioRxiv - Microbiology 2022Quote: 10 OD of log phase cells was harvested and resuspended in phenol solution (P4682; Sigma-Aldrich), SDS ...
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bioRxiv - Microbiology 2022Quote: ... midlog phase bacteria were washed with PBS and resuspended in 0.5 mg/ml FITC (Sigma Aldrich) in PBS for 1 h on ice ...
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bioRxiv - Neuroscience 2021Quote: Colonies at selected phases (Tables 1-4) were anaesthetized with MS222 (tricaine methanesulfonate, Sigma, A5040-25) and fixed in 4% PFA in MOPS (0,1 M MOPS ...
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bioRxiv - Cancer Biology 2019Quote: ... were UV-ozone treated and silanized through vapor phase deposition of (3-aminopropyl)triethoxysilane (Sigma-Aldrich) at 90 °C for a minimum of 18 hours ...
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bioRxiv - Genomics 2021Quote: ... 0.5mL of homogenized mixture was phase separate by the addition of 100μL of chloroform (Sigma, C2432) and mixed by inversion ...
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bioRxiv - Physiology 2021Quote: ... The aqueous phase was transferred to a new tube containing 250μl isopropanol (Sigma-Aldrich, Poole, UK) and centrifuged as above ...
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bioRxiv - Bioengineering 2020Quote: ... The oil phase was a heavy mineral oil (Fisher) containing 0.25% v/v Span-80 (Sigma). Downstream of the pinching region ...
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bioRxiv - Molecular Biology 2021Quote: ... Progression into S phase was monitored microscopically by counting cells that contained septa using calcofluor (Sigma) staining ...
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bioRxiv - Cell Biology 2021Quote: Cells were synchronized into S phase by double thymidine block and release: 2mM thymidine (Sigma-Aldrich) was added to culture medium for 24 hours ...
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bioRxiv - Biophysics 2022Quote: ... The aqueous phase was retained and transferred into siliconized centrifuge tubes (Sigma-Aldrich Cat. No. T2418) and agitated for 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were first arrested in S phase by the addition of 2 mM thymidine (Sigma-Aldrich) for 19 h ...
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bioRxiv - Systems Biology 2023Quote: ... The initial mobile phase was 10 mM ammonium formate (pH 7.0) and pure acetonitrile (Merck Millipore) in the ratio of 95 to 5 and maintained for 8 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... The viral phase was isolated by iodixanol step gradient (15%, 25%, 40%, 60% Optiprep, Sigma-Aldrich) in the 40% fraction and concentrated in PBS (Phosphate Buffer Saline ...
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bioRxiv - Cell Biology 2023Quote: The phase separation behavior of hIRE1α LD protein variants in presence of PEG (Sigma-Aldrich (P2139) or 40 % (w/w ...
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bioRxiv - Cell Biology 2024Quote: ... and the peptide mixtures were desalted on C-18 reverse phase material (ZipTip μ-C18, Millipore). Eluted peptides were concentrated by evaporation under vacuum and subsequently resolved in 0.1% acetic acid / 2% acetonitrile containing HRM/iRT peptides (Biognosys ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells where then lysed with BugBuster protein extraction reagent (Novagen) for 30 min at room temperature ...
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Therapy-induced lipid uptake and remodeling underpin ferroptosis hypersensitivity in prostate cancerbioRxiv - Cancer Biology 2020Quote: ... Metabolites were extracted using 80% methanol in water extraction buffer (Sigma) that was supplemented with uniformly deuterated myristic acid (2 µM ...
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bioRxiv - Plant Biology 2021Quote: ... RNA extraction was performed with Spectrum Plant Total RNA Kit (Sigma). DNase I (New England Biolabs ...
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bioRxiv - Plant Biology 2021Quote: ... By using the DNA Extraction Kit (SIGMA®, Saint Louis, MO), DNA was extracted for soybean genotypes ...
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bioRxiv - Cell Biology 2021Quote: ... Protein extractions were electroblotted on a polyvinylidene fluoride (PVDF) membrane (Millipore). Membranes were blocked with 5% nonfat dry milk in TBST for 1h and probed with 1:1000 anti-p53 (cst ...
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bioRxiv - Microbiology 2022Quote: ... Trizol™ RNA extraction carried out as per manufacturer’s instructions (Sigma).
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... Protein extraction buffers contained 50mM HEPES (Sigma Aldrich, H0887-100 ml) and 150 mM NaCl ...
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bioRxiv - Neuroscience 2022Quote: ... Cell pellets were then resuspended in RIPA protein extraction buffer (Sigma) supplemented with protease inhibitor (Roche ...
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bioRxiv - Neuroscience 2020Quote: ... RNA extraction was performed using Trizol (TRI reagent, Sigma-Aldrich, USA). The RNA was quantified using a Nanodrop (Thermo Scientific ...
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bioRxiv - Genomics 2021Quote: ... The eluate was processed for RNA extraction using acid-phenol:chloroform (Sigma) and added to the mirVana miRNA (Applied Biosystems ...
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bioRxiv - Genetics 2020Quote: ... Protein extraction and Western blot using anti-puromycin antibody (Merck Millipore) was performed as described before.
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bioRxiv - Cancer Biology 2021Quote: Total RNA was extracted using an RNA extraction kit (Sigma-Aldrich) according to the provided manual ...
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bioRxiv - Microbiology 2021Quote: ... Protein extraction was performed in the presence of 2% SDS (Sigma) and 4 M acetic acid (Fisher ...