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Citations for Millipore Sigma :
601 - 650 of 1090 citations for Solid Phase Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... in mid-logarithmic phase was added at a multiplicity of infection of 2 in RPMI with 10% human serum (Sigma). Bacteria were spun down for 5 min at 800 x g and incubated for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Dried extracts were phase-separated using -20°C LCMS-grade chloroform/methanol/water (1:3:3, v/v) (Sigma Aldrich).
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bioRxiv - Plant Biology 2023Quote: ... A binary gradient was used for peptide elution, where mobile phase A consisted of water (Tedia, Fairfield, Ohio, USA) with 0.1% formic acid (Sigma-Aldrich), and mobile phase B consisted of acetonitrile (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... and the supernatant obtained was spotted 2 μl on the origin point of a reversed-phase plate (RP-18, Merck Millipore) and developed with a mixture solution of acetonitrile ...
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bioRxiv - Biochemistry 2023Quote: ... and determination of the released glycerol in 10 µl of the upper phase with a commercial Glycerol Assay Kit according to the manufacturer’s instructions (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2023Quote: ... Clear phase on top was transferred to a new 1.5 ml tube and 1 µl glycogen (20 mg/ml, Sigma, missing) added ...
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bioRxiv - Cancer Biology 2023Quote: ... The mobile phases consisted of deionized water and HPLC grade acetonitrile with 0.1% LCMS grade formic acid obtained from Sigma Aldrich and Fisher Scientific.
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bioRxiv - Neuroscience 2023Quote: ... we applied with unlabeled (S)-PhPrM and (S)-PhPr solutions to the origin point on a reversed-phase plate coated with fluorescent indicator (RP-18 F254S, Merck Millipore). The developed spots were detected with ultraviolet light (254 nm).
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bioRxiv - Neuroscience 2023Quote: ... brain homogenate from a RML inoculated C57BL6/J mouse at the terminal phase of disease was treated with 100 μg/ml of pronase E (Sigma) for 30 min at 37 °C with shaking in a thermal mixer at 800 rpm ...
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bioRxiv - Biochemistry 2024Quote: ... the reactions were desalted by ion pairing reverse phase (IP-RP) purification using C18 ZipTip pipette tips (Millipore, Billerica, MA) to avoid smearing of bands in PAGE ...
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bioRxiv - Cell Biology 2020Quote: ... and nucleoproteins were extracted using a nuclear protein extraction kit (Merck Millipore, Darmstadt, Germany). Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS ...
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bioRxiv - Plant Biology 2020Quote: ... The extraction buffer was then collected and filtered through Miracloth (Merck Millipore, MA, US). Three volumes of precooled acetone were added to the extract ...
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bioRxiv - Microbiology 2019Quote: ... Bacterial genome was extracted using DNA extraction kit (Sigma-Aldrich, St. Louis, MO, USA). Whole-genome sequencing was conducted on the Illumina HiSeq platform with genome coverage (sequencing depth ...
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bioRxiv - Plant Biology 2019Quote: ... 1 mL of protein extraction was applied to M5 anti-FLAG magnetic beads (Sigma). The beads were then boiled and the resulting fraction was run on a 12% SDS-PAGE gel ...
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bioRxiv - Plant Biology 2021Quote: ... The pellet was dried and resuspended in Protein Extraction Reagent (C0856, Sigma-Aldrich®) supplemented with 1% Protease inhibitor (P9599 ...
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bioRxiv - Microbiology 2020Quote: ... coli DS1 using the GenElute Bacterial Genome Extraction Kit (Sigma-Aldrich, St. Louis, USA) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2021Quote: Extraction of protein from isolated exosomes was performed using SDS2.5%/8M urea (Sigma-Aldrich) lysis buffer ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using the kit Gene eluteTM Gel extraction kit (Sigma Aldrich), re-surveyed on Nanodrop® and delivered for Sanger DNA sequenced at Eurofins Genomics (Ebersberg ...
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bioRxiv - Cancer Biology 2020Quote: ... and membrane fractions were isolated using a Compartment Protein Extraction Kit (Millipore, Billerica, MA) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: Leaf RNA extractions were performed using the Spectrum Plant Total RNA Kit (Sigma-Aldrich). RNA extractions from developing inflorescences were performed using the RNeasy Plant Mini Kit (Qiagen) ...
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bioRxiv - Plant Biology 2019Quote: RNA extraction was performed using the Spectrum™ Plant Total RNA kit (Sigma-Aldrich), followed by DNase I treatment (amplification grade DNase I ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed once in PBS and scraped in phosphosafe extraction reagent (Merck Millipore) supplemented with 1x protease inhibitor cocktail (Roche).
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bioRxiv - Cell Biology 2022Quote: ... extraction and AP protocols are supplemented with EDTA-free COMPLETE protease inhibitors (Millipore Sigma) to minimize protein degradation ...
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bioRxiv - Cell Biology 2022Quote: ... (ii) Pre-extraction solution: PEM buffer supplemented with 0.3% Triton X-100 (Sigma, 93443) and 0.125% Glutaraldehyde (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... and cells were lysed with 50 μL of 1 × BugBuster Protein Extraction Reagent (Sigma). Lysates clarified at 21 ...
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bioRxiv - Microbiology 2022Quote: ... individual amplicons were excised and extracted with the GenElute Gel Extraction Kit (Sigma-Aldrich) according to manufacturer’s instructions.
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bioRxiv - Plant Biology 2022Quote: Plant nuclei were isolated from plants using the CelLytic PN Isolation/Extraction Kit (Sigma) and nine leaf discs (0.75 cm in diameter ...
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bioRxiv - Microbiology 2022Quote: ... or manual extraction kits (Invitrogen PureLink genomic DNA kit or the Sigma GenElute kit). Normalised DNA (at a concentration of 0.2 ng/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was purified by two extractions with Phenol:Chloroform:Isoamyl-alcohol (25:24:1, Sigma Aldrich) and precipitated by adding it to 2 µl of glycogen ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted using the GenElute Bacterial Genomic DNA Extraction kit (Sigma-Aldrich). PCR products were purified using the QuickClean 5M PCR Purification Kit (GenScript ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted according to the Sigma RNA Extraction Kit (#RTN350-1KT, Sigma) protocol ...
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bioRxiv - Plant Biology 2023Quote: ... 100 mg of plant tissue was used for RNA extraction with TRIzol (Sigma Aldrich) reagent ...
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bioRxiv - Plant Biology 2024Quote: ... Genomic DNA was extracted from each plant using Extraction and Dilution buffers (Sigma-Aldrich, E7526 and D5688 ...
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bioRxiv - Plant Biology 2024Quote: ... DNA extraction was performed using a Phenol-Chloroform-Isoamyl alcohol mixture (Sigma, Cat# 77617), and the DNA was resuspended in 200 µL of ddH2O ...
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bioRxiv - Neuroscience 2020Quote: ... This mixture was transferred to a pre-spun 2-mL heavy phase lock gel (5 Prime) after which 200μL of chloroform/isoamyl alcohol (49:1, v/v, Sigma cat# 25668) was added and samples were vortexed vigorously ...
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bioRxiv - Neuroscience 2020Quote: ... Sulfatides were separated by reverse phase LC using a Supelco 2.1 (i.d.) x 50 mm Ascentis Express C18 column (Sigma, St. Louis, MO) and a binary solvent system at a flow rate of 0.7 mL/min with a column oven set to 60°C ...
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bioRxiv - Neuroscience 2020Quote: These compounds were separated by reverse phase LC using a Supelco 2.1 (i.d.) x 50 mm Ascentis Express C18 column (Sigma, St. Louis, MO) and a binary solvent system at a flow rate of 0.5 mL/min with a column oven set to 35°C ...
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bioRxiv - Neuroscience 2020Quote: ... The lipids were separated by reverse phase LC using a Supelco 2.1 (i.d.) x 50 mm Ascentis Express C18 column (Sigma, St. Louis, MO) and a binary solvent system at a flow rate of 0.5mL/min with a column oven set to 35°C ...
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bioRxiv - Immunology 2020Quote: ... all CIS43 VLPs and wild-type (WT) Qβ VLPs were depleted of endotoxin by three rounds of phase separation using Triton X-114 (Sigma-Aldrich), as described in reference 48 ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was purified by collecting and transferring the trizol/chloroform upper aqueous phase to a new tube and the RNA precipitated with 750 μl of isopropanol (Sigma Aldrich) for 10 min at 16000 g ...
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bioRxiv - Microbiology 2019Quote: ... Ethanol was mixed with the aqueous phase and RNA isolation protocol was continued as per GenElute Mammalian Total RNA Miniprep kit (RTN350, Millipore Sigma) protocol ...
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bioRxiv - Cell Biology 2021Quote: ... the cantilevers were cleaned by a 90 s plasma treatment (Plasma Cleaner PDG-32G, Harrick Plasma) before coating overnight with vapor phase SL2 Sigmacote (Sigma-Aldrich) in a vacuum desiccator ...
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bioRxiv - Biochemistry 2021Quote: ... at a flow rate of 10 µL/min for 4 min in an isocratic mode of Mobile Phase C (2% acetonitrile (Sigma-Aldrich), 0.1% formic acid) ...
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bioRxiv - Molecular Biology 2022Quote: ... Total protein extracts were obtained from 150 mL cultures grown to early stationary phase by resuspending in X mL of 0.2 M sorbitol (Sigma-Aldrich, USA), 1 % β-mercaptoethanol ...
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bioRxiv - Neuroscience 2021Quote: ... coupled to an electrochemical detector (Decade Lite-Antec Scientific) with a 5-μm Spherisorb ODS-2 reversed-phase column (Sigma-Aldrich) and the results were expressed as norepinephrine concentration in ng/g of tissue.
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bioRxiv - Cell Biology 2021Quote: ... grown at least for 4 hours to mid-log phase and plated in minimal synthetic medium on concanavalin A–coated (Sigma-Aldrich) Lab-Tek chambers (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2022Quote: ... cells were grown overnight to exponential phase and serial dilutions (1/10) of cells were spotted on agar plates using a replica plater (Sigma R2383). Plates were photographed after 2–5 days at 30°C.
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bioRxiv - Microbiology 2022Quote: ... DNA from the top aqueous phase (600 μL) was precipitated on an addition of 2-propanol (600 μL; Sigma-Aldrich, USA). Then ...
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bioRxiv - Microbiology 2022Quote: ... Isopropanol was mixed with the aqueous phase and RNA was isolated as per the GenElute Mammalian Total RNA Miniprep kit (RTN350, Millipore Sigma) protocol ...
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bioRxiv - Bioengineering 2019Quote: ... The oil phase with the fluorinated surfactant was removed by adding 10% 1H,1H,2H,2H-Perfluoro-1-octanol (Sigma-Aldrich) in HFE 7500 (14 ...