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Citations for Millipore Sigma :
651 - 700 of 1090 citations for Solid Phase Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... Osteoclast generation was verified directly by phase contrast microscopy or after staining for Tartrate-Resistant Acid Phosphatase (TRAP) (Leukocyte Acid Phosphatase kit, Sigma-Aldrich). Human TNFα and CCL2 in M-MØ culture supernatants were measured using commercially available ELISA (BD Biosciences).
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bioRxiv - Immunology 2020Quote: ... added to a mix of irradiated feeders consisting of freshly isolated allogeneic PBMC combined with log-phase-growth RPMI 8866 cells (Sigma-Aldrich) at a 10:1 ratio in cloning medium ...
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bioRxiv - Cancer Biology 2020Quote: cells were arrested at S phase by first arresting cells at G1 phase then replacing the medium with growth medium supplemented with serum and Hydroxyurea (Sigma, UK) at a final concentration of 1mM and incubated overnight.
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bioRxiv - Molecular Biology 2021Quote: ... Successfully transformed cells were cultured in YPD media and eventually arrested in late G1 phase by the addition of alpha factor (Sigma-Aldrich). Cells were washed and resuspended in YPGal to induce the expression of wild-type telomerase ...
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bioRxiv - Synthetic Biology 2020Quote: ... and started with a batch phase using 400 mL synthetic medium containing 20 g/L of glucose and 5 mL Antifoam 204 (Sigma A6426). The temperature was maintained at 30°C and the pH was kept constant at 5.0 by dropwise addition of a 10M KOH solution ...
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bioRxiv - Systems Biology 2019Quote: ... Identification of metabolites from organic phase GC-MS analysis was supported by comparison with a FAME standard mix (Supelco 37 Component FAME Mix; Sigma Aldrich).
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bioRxiv - Biochemistry 2021Quote: All separations were performed isocratically at 25°C with a mobile phase comprised of 50 mM TIP solution buffered to pH 3.5 via phosphoric acid (99% Sigma-Aldrich, Australia). The flow rate was maintained at 0.4 mL/min ...
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bioRxiv - Plant Biology 2022Quote: ... 250 ml log-phase culture cells were removed by centrifugation and the supernatant filtered through 0.22 µm membranes (Millipore, Billerica, MA). Solid phase extractions of the supernatants were done using benzene sulphonic acid cation exchanger on silica (Macherey Nagel ...
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bioRxiv - Microbiology 2020Quote: ... Sample desalting was performed with home-made StageTips consisting in a stack of two reverse-phase C18 layers (Empore SPE Disks C18, Sigma Aldrich) inserted in a 10 μl tip ...
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bioRxiv - Microbiology 2021Quote: Hydrolysis of Insulin B Chain was measured via reverse phase high performance liquid chromatography (HPLC) following incubation of TLN4 (160 μg/mL) with insulin B chain (42 μM, Sigma Aldrich) in 10 mM Tris for 88 h ...
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bioRxiv - Molecular Biology 2020Quote: ... The mobile phases were prepared with two solutions: (A) 10 mM ammonium formate and 0.125% formic acid (Sigma, St. Louis, MO) in ultrapure water and (B ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were grown overnight to exponential phase and five serial dilutions (1/4) of cells were spotted on agar plates using a replica plater (Sigma R2383). Plates were incubated at the relevant temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were then washed once in YPD or SC medium and released in S phase in medium supplemented with 5 µg/mL pronase (Protease from Streptomyces griseus, Sigma-Aldrich). For ionizing irradiation ...
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bioRxiv - Genomics 2022Quote: ... a single thymidine block was used to first arrest cells in S phase: cells were plated at 4×10^6 cells per 15 cm plate in medium containing 2mM Thymidine (Sigma, T1895), and were grown for 24 hours ...
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bioRxiv - Microbiology 2022Quote: ... Cells were sampled from the mid log phase of growth and stained by addition of FM4-64 (Sigma-Aldrich, Mumbai, India) and grown for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... The morphology of cells from well-fed cultures and starved cultures was compared using phase contrast microscopy and a qualitative determination (presence/absence) of PHA was done by staining with Nile Red (Sigma-Aldrich) followed by fluorescence microscopy ...
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bioRxiv - Molecular Biology 2022Quote: ... at a constant flow rate of 0.8 ml/min and a constant temperature of 22°C with mobile phase A being 0.4 % acetic acid (Sigma-Aldrich, USA) in water and phase B being 20% Methanol (Honeywell ...
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bioRxiv - Neuroscience 2023Quote: ... Sample desalting was performed with home-made StageTips consisting in a stack of two reverse-phase C18 layers (Empore SPE Disks C18, Sigma Aldrich) inserted in a 10 μL tip ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting upper aqueous phase was transferred to a new tube and mixed with 500 µL of molecular grade 2-propanol (Sigma, I9516). Samples were incubated 10 minutes at room temperature and centrifuged at 12,000 g for 10 minutes at 4°C to precipitate the RNA ...
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bioRxiv - Cell Biology 2023Quote: ... A 2 µL sample was injected into a normal phase Ascentis® Express HILIC UHPLC column (2.1 x 100 mm, 2 µm particle size; Sigma, USA) and eluted at 30°C over a 20-minute gradient with a flow rate of 400 µL/min ...
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bioRxiv - Microbiology 2023Quote: ... Stationary phase cultures (37°C, 18-24 h, with shaking) were washed twice with sterile phosphate buffered saline (PBS; D8537, Sigma-Aldrich) and diluted to 5 x 105 CFU.mL−1 for experiments ...
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bioRxiv - Microbiology 2024Quote: ... cells were grown in YNB media and collected on log phase to subsequently treat them with or without hydrogen peroxide solution (H2O2, 5 mM; Sigma-Aldrich) for 1 hour at 30°C ...
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bioRxiv - Molecular Biology 2023Quote: ... the clear phase was transferred to a fresh tube and mixed with 500 μl of isopropanol (catalog no. 534021, Sigma-Aldrich). The samples were centrifuged again ...
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bioRxiv - Cell Biology 2024Quote: ... Approximately 20 x 107 HeLaK cells pre- synchronized by thymidine treatment (2 mM for 18 h) were released to undergo S-phase in presence of F-ara-Edu (5 µM; Sigma-Aldrich) for 5 h ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... During place preference training water in one corner (less visited corner during adaptation phase) was replaced with sucrose solution (5%, Sigma-Aldrich). During preference extinction ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclei extraction was performing using the Nuclei Isolation Kit: Nuclei PURE Prep Kit (Millipore Sigma) according to the manufacturer’s instructions with the following modifications ...
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bioRxiv - Immunology 2021Quote: ... 1mL of metabolite extraction solution (stored at −80C) consisting of 50% Methanol stock (Sigma 322415), 30% Acetonitrile stock (Fisher A998N1) ...
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bioRxiv - Biophysics 2020Quote: ... The pellet was resuspended in extraction buffer containing 10 mM Mg-ATP (#A9187, Sigma-Aldrich) for 10 minutes at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... The bacterial pellet was lysed in 2 mL 1× BugBuster™ protein extraction reagent (Novagen), incubated by slow shaking for 20 min ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA extraction used the Spectrum™ Plant Total RNA Kit (Sigma Aldrich, Darmstadt, Germany), following protocol “A” ...
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bioRxiv - Neuroscience 2020Quote: ... Embryos were then placed in RIPA extraction buffer (Sigma-Aldrich Corp. St. Louis, Missouri, USA) containing Complete Proteinase Inhibitor (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2021Quote: The lyophilisates were resuspended in the extraction buffer (300 μL) containing 4% SDS (Sigma-Aldrich) and 0.1 mol/L 1,4-dithiothreitol (Roche ...
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bioRxiv - Neuroscience 2019Quote: ... EBs were collected for RNA extraction with the GenElute Mammalian Total RNA kit (Sigma, RTN350). cDNA synthesis was performed with SuperscriptTM III (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... RNA extraction were performed using the Spectrum™ Plant Total RNA Kit (Sigma Aldrich, USA) following the manufacturer’s protocols including the optional on-column DNase 1 digestion (DNASE10-1SET ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was extracted from Arabidopsis root samples using a RNA extraction kit (Sigma Aldrich) and quality control was performed photometrically using a NanoDrop (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... Total protein was extracted using an extraction buffer comprised of 50 mM MOPS (M1254, Sigma) pH 7.0 ...
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bioRxiv - Microbiology 2020Quote: The DRM and DSM fractions119 were separated using the CelLytic MEM Protein Extraction Kit (Sigma). 600 μl of lysis and separation buffer were added to 50 μl of resuspended membrane and incubated at 4°C overnight ...
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bioRxiv - Cell Biology 2020Quote: Proteins of cellular membrane were extracted using the ProteoExtract® Transmembrane Protein Extraction Kit (NOVAGEN). Total 500 μg of membrane proteins were used for immunoprecipitation with an anti AT1-R or LOX-1 antibody ...
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bioRxiv - Physiology 2021Quote: ... The remaining supernatant was precipitated using the Calbiochem Protein Extraction Kit (EMD Millipore, Billerica, MA) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... RNA extraction was performed by addition of 1/10th volume bromochloropropane (Sigma Aldrich, Cat# B9673) to the tri-reagent and then vortexed and incubated at room temperature for 5 minutes and then spun at 12,000 x g for 5 minutes to achieve phase separation ...
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bioRxiv - Microbiology 2019Quote: ... The product was purified from 1% agarose gel with GenElute Gel Extraction Kit (Sigma-Aldrich) and cloned into pJET1.2/blunt vector using CloneJET PCR Cloning Kit (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2020Quote: ... These pellets were resuspended and lysed in 100 µL of Bugbuster protein extraction reagent (Millipore) containing 5 U mL−1 Benzonase nuclease (Millipore ...
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bioRxiv - Systems Biology 2020Quote: ... 150μL of extraction buffer (0.1% Trition-X100 (Sigma-Aldrich; Cat#X100; CAS: 9002-93-1) and 0.6% sulfosalicylic acid (Sigma-Aldrich ...
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bioRxiv - Pathology 2020Quote: Tissue slices were lysed with extraction buffer containing 25 mM Tris (Sigma-Aldrich, Missouri, US), 10 mM sodium phosphate (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2021Quote: ... 750 µl/300 µl of extraction buffer (100% methanol plus the internal standard adonitol, Sigma) were added to root and leaf samples respectively ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatants were desalted using homemade C18 stage tips containing 3M Empore extraction disks (Sigma) and 5 mg of Poros R3 resin (Applied Biosystems) ...
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bioRxiv - Cell Biology 2021Quote: ... 150 µL of cold extraction solution (40% (v/v) HPLC-grade acetonitrile (Sigma-Aldrich: 34998), 40% (v/v ...
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bioRxiv - Cell Biology 2020Quote: Proteins from 3T3-L1 fibroblast lysates extracted with Cytobuster protein extraction reagent (Novagen®, Merck). After cell disruption for 10 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... tonsil using a ProteoExtract Complete Mammalian Proteome Extraction Kit (cat#539779, Merck Millipore, Darmstadt, Germany), following tissue homogenization with a Precellys 24 (Bertin Instruments ...
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bioRxiv - Neuroscience 2021Quote: ... the isolated cell populations were immediately placed in 20μl RIPA protein extraction buffer (EMD Millipore) with HALT Protease/Phosphatase inhibitors (ThermoFisher Scientific ...