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Citations for Millipore Sigma :
51 - 100 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 800 1000 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... coli using a Nickel-His column (Sigma). A 5 mL culture was incubated at 37°C for 16 hours ...
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bioRxiv - Cancer Biology 2021Quote: LPS derived from Escherichia coli (E. coli) was from Sigma-Aldrich Canada (Oakville ...
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bioRxiv - Microbiology 2023Quote: ... coli strain BL21 (DE3) (E. coli BL21, EMD Millipore, Cat#69450). F ...
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bioRxiv - Immunology 2020Quote: ... injection of either PBS or 2 mg/kg LPS (Sigma (E. coli 055:B5)) dissolved in PBS ...
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bioRxiv - Microbiology 2020Quote: ... as a His-tag fusion in pET28b (Novagen). Cells were grown in Terrific-Broth at 37°C to an OD600 = 1.2 and after 15 min at 4°C induced with 1 mM isopropyl β-d-thiogalactoside ...
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bioRxiv - Pathology 2021Quote: ... cloned into a pET28a His-tag vector (Novagen) and expressed in E ...
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bioRxiv - Cell Biology 2021Quote: SARS-CoV-2-infected and mock-treated hiPSC-CMs were fixed using 4% paraformaldehyde solution (Sigma-Aldrich, EUA) for 1 hour and stored at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Mouse monoclonal antibody MAB8150 against WNV E glycoprotein was from EMD Millipore (Billenca MA). Alexa Fluor 488 goat anti-mouse IgG (H+L ...
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bioRxiv - Cancer Biology 2019Quote: E coli Rosetta (DE3) pLysS cells (Novagen) transformed with pOPIN-B plasmids encoding wtGATA3 and neoGATA3 were grown in autoinduction media supplemented with 50 µg/ml kanamycin and 34 µg/ml chloramphenicol for 8 h at 37 °C followed by 21 h at 20 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins with His tag were purified using Ni-NTA His binding resin (Novagen) and those with GST tag was purified by glutathione sepharose (Novagen).
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bioRxiv - Biochemistry 2023Quote: Escherichia coli (E. coli) Rosetta2(DE3)pLysS cells (Novagen, cat. no. 71403) were transformed with pET28a-MDA5ΔL2-His6 and grown in 2xTY medium to OD600 0.4-0.6 at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: SARS-CoV-2 was diluted to the desired amounts in RDM supplemented with 100 μM of taurine (Sigma-Aldrich) without FBS ...
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bioRxiv - Microbiology 2021Quote: ... and a SARS-CoV-2 reporter vector (50 ng) and 1.5 μL of X-tremeGENE 9 transfection reagent (Sigma) and incubated for 48 hrs ...
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bioRxiv - Microbiology 2020Quote: ... HCoV-NL63 and SARS-CoV-2 were supplemented with 1 µg/ml TPCK-treated trypsin (Sigma-Aldrich, cat. #T1426) increasing the rate of infection ...
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bioRxiv - Immunology 2020Quote: ... pTT3-SARS-CoV-2-S was transfected into 293E cells using 293 Free transfection reagent (EMD Millipore Cat # 72181) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... were cultured in 24 well plates and stimulated with inactivated SARS-COV-2 antigen (1.2 μg/ml) or PMA (25 ng/ml, cat # P8139; Sigma) and Ionomycin (1 μg/ml ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 S expression HEK293T cells were stained with PKH26 dye according to the manufactures protocol (Sigma-Aldrich). Next ...
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bioRxiv - Immunology 2022Quote: ... the cells were immunostained with a mouse monoclonal antibody against SARS-CoV-1/2 nucleoprotein [clone 1C7C7 (Sigma-Aldrich)] ...
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bioRxiv - Microbiology 2023Quote: Whole lungs were isolated from SARS-CoV-2 infected mice and homogenized in 600 μL TRIzol (Sigma-Aldrich, MA) with 3.2 mm stainless steel beads (NextAdvance ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used for immunoblotting included anti-SARS-CoV-2 N (SinoBiological 40143-R001) and GAPDH (Millipore-Sigma G8795) monoclonal antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used for immunoblotting included anti-SARS-CoV-2 N (SinoBiological 40143-R001) and GAPDH (Millipore-Sigma G8795) monoclonal antibodies ...
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bioRxiv - Genomics 2022Quote: ... Infectious foci were stained with a human anti-SARS-CoV-2 Spike antibody (H2-162, Hugo Mouquet’s laboratory, Institut Pasteur) and the corresponding HRP-conjugated secondary antibody (Sigma-Aldrich). Foci were visualized by 3,3’-diaminobenzidine staining solution (DAB ...
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bioRxiv - Biophysics 2020Quote: ... The (His)8-tag was cleaved by thrombin (Sigma) using 20 U per 1 mg GltPh in the presence of 5 mM CaCl2 at room temperature overnight ...
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bioRxiv - Microbiology 2023Quote: hPCLS were infected with SARS-CoV-2 at 2×105 TCID50 per slice and treated with homoharringtonine (HHT) (Sigma, cat# SML-1091-10MG) at 1 h p.i ...
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bioRxiv - Biochemistry 2022Quote: ... 50ng/mL LPS (E. coli O111:B4, Sigma) and 10ng/mL IFN-γ (R&D Systems ...
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bioRxiv - Microbiology 2019Quote: ... ampicillin (Sigma; 100 μg/mL for E. coli), gentamicin (Sigma ...
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bioRxiv - Neuroscience 2020Quote: LPS (Sigma Aldrich, E. coli, serotype 0111:B4), was prepared by dissolving 0.3 mg of LPS in 1ml of PBS (Phosphate buffer saline) ...
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bioRxiv - Bioengineering 2022Quote: ... and LPS (lipopolysaccharides from E. coli; Sigma L8274); HEK-293Ta showed the strongest response to TNFα and were thus selected as responder cells in the co-culture assay (Fig ...
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bioRxiv - Microbiology 2022Quote: ... or tRNA (from E. coli MRE 600, Sigma) was incubated with PT mutants ...
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bioRxiv - Microbiology 2019Quote: ... anti-His (1:1000 Sigma), or anti-StrepII (1:3000 IBA ...
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bioRxiv - Biophysics 2021Quote: ... The protein was expressed and purified the same way as SARS-CoV Mpro-2 but Factor Xa protease (Sigma, Canada) was used (4 °C ...
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bioRxiv - Microbiology 2020Quote: Active and pseudotyped SARS-CoV-2 particles were immobilized by adding 0.01% Poly-L-Lysine (PLL, Sigma Aldrich, MIS, USA) for 30 min ...
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bioRxiv - Microbiology 2021Quote: Fifty microliters containing 3.5 x 106 PFU/ml SARS-CoV-2 viral stock was placed on one well of a 4-well chambered glass slide (Nunc, Sigma) (Fig 2) ...
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bioRxiv - Microbiology 2020Quote: A549 cells infected with arboviruses or ACE2-SK-N-SH cells infected with SARS-CoV-2 (MOI=0.05-5) were treated with the RIPK2 inhibitor GSK583 (28) (Sigma-Aldrich) for 24 hours ...
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bioRxiv - Microbiology 2022Quote: ... the Milliplex SARS-CoV-2 Antigen Panel 1 IgG was used according to the manufacturer’s instructions (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Microbiology 2021Quote: ... We prepared a solution of SARS-CoV-2 in Dulbecco’s Modified Eagle Medium cell culture medium (Sigma-Aldrich, reference D6546) supplemented with 2 nM L-glutamine ...
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bioRxiv - Immunology 2020Quote: ... 400 μl containing 300 PFU/ml of SARS-CoV-2 virus were then added to the mAb solution supplemented with 0.25% guinea pig complement sera (Sigma, Israel) and the mixture incubated at 37°C ...
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bioRxiv - Immunology 2020Quote: ... 0.5 mL of SARS-CoV-2 S-2P protein was mixed with either an equal volume of Sigma Adjuvant System S6322 (Sigma), 600 μg/mL Adju-Phos aluminum phosphate (Brenntag) ...
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bioRxiv - Immunology 2021Quote: ... 400 μl containing 500 PFU/ml of SARS-CoV-2 virus were then added to the Ab solution supplemented with 0.25% guinea pig complement sera (Sigma, Israel) and the mixture incubated at 37°C ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... CHO CoV-2-Spike cells were used as target cells and were fluorescently labeled with PKH67 Fluorescent Cell Linker Kit (Sigma Aldrich) prior to incubation with mAbs ...
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bioRxiv - Bioengineering 2019Quote: The His-TEV tag was cleaved using TEV protease (Sigma). The reaction was made overnight at 4°C into dialysis buffer for TEV (25 mM Tris-HCl ...
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bioRxiv - Immunology 2020Quote: ... Blotted proteins were detected with a monoclonal antibody that recognizes SARS-CoV-2 S1 (SinoBiological) and a secondary anti-rabbit horse radish peroxidase (HRP)-conjugated antibody (Sigma Aldrich). Blots were developed with Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ADCP assay was performed using ExpiCHO cells stably expressing full-length wild-type SARS-CoV-2 S (target cells) and labelled with PKH67 (Sigma Aldrich) as targets ...
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bioRxiv - Immunology 2022Quote: ... 1 – 1.5 million cells were plated into 96-well V-bottom plates in 200mL R10 and stimulated with SARS-CoV-2 peptide pools (2ug/mL for each peptide) in the presence of Brefeldin A (Sigma-Aldrich) and monensin (GolgiStop ...
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Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: ... and analyzed by western blot with anti-SARS-CoV-2 N protein monoclonal antibody (0.5 μg/mL, anti-SARS-CoV-2 N mAb, clone 1G10C4 mAb)42 and anti-β-actin antibody (Sigma-Aldrich, cat ...
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bioRxiv - Immunology 2020Quote: Alexa fluor 488 labelled bacteria (E. coli, Sigma-Aldrich) and yeast (S ...
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bioRxiv - Immunology 2021Quote: ... LPS (E. coli serotype O55:B5, Sigma, 10ng/ml), CpG ODN 1826 (TIB MOLBIOL ...
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bioRxiv - Neuroscience 2023Quote: LPS (E. coli, O111:B4 serotype, Sigma Aldrich, UK) was dissolved at 0.5 mg/ml in sterile 0.9% saline ...
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bioRxiv - Biochemistry 2024Quote: ... for 2 h before stimulation with 100 ng/mL lipopolysaccharide (from E. coli O55:B5, Sigma Aldrich), with four biological replicates per condition ...