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Citations for Millipore Sigma :
1 - 50 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 800 1000 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... using cross-reactive anti-SARS-CoV-1 Spike CR3022 or mouse anti-His tag (Sigma-Aldrich) as positive controls ...
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bioRxiv - Biochemistry 2023Quote: Purified SARS-CoV-2 spike glycoproteins were exchanged to water using Microcon Ultracel PL-10 centrifugal filter (Millipore Sigma). Glycoproteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl ...
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bioRxiv - Microbiology 2022Quote: ADCP assays were performed using ExpiCHO-S cells transiently transfected with SARS-CoV-2 spike glycoproteins (Wuhan D614, BA.1, or BA.2) and labelled with PKH67 (Sigma Aldrich) as targets ...
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bioRxiv - Microbiology 2021Quote: ... for SARS CoV-2 spike detection mouse anti-spike (Cat# ZMS1076, Sigma Aldrich) respectively ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 spike protein (S1) (#E5S3V) and GAPDH (G9545, Sigma-Aldrich) were incubated overnight at 4 °C in TBS-T ...
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bioRxiv - Microbiology 2020Quote: ... rabbit polycolonal anti-SARS-CoV-2 S2 antibodies (1:1000) (Sinobiological Inc, Beijing, China) and anti-FLAG M2 antibody (1:1000) (Sigma, St. Louis, MO, USA), respectively.
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bioRxiv - Microbiology 2021Quote: Six-week-old Balb/cN mice were primed subcutaneously either with 30 μg of recombinant SARS-Cov-2 Spike protein (S) or Spike-RBD (RBD) in the complete Freund’s adjuvant (SIGMA-ALDRICH) and boosted three times at three-week intervals with 20 μg of the same antigen in the incomplete Freund`s adjuvant ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 Spike expressing expi293F cells were stained with PKH26 (Sigma-Aldrich, St-Louis, MO). Cells were then washed with and resuspended in R10 media ...
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-COV-2 Spike protein was desalted and concentrated with C4 ZipTips (Merck Millipore, Darmstadt, Germany) and spotted on a ground steel target using 2’,5’-Dihydroxyacetophenone ...
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bioRxiv - Immunology 2020Quote: ... Detection of the SARS CoV-2 spike protein was achieved by incubation with an anti-rabbit (Sigma) secondary antibody ...
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bioRxiv - Biochemistry 2023Quote: ... His tag and Flag tag (1:1000; F3165, Sigma).
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bioRxiv - Microbiology 2021Quote: ... and 0.5 µg pcDNA3.1 SARS-CoV-2 Spike D614 in OptiMEM with 10 µl polyethyleneimine 1 µg/ml (Sigma) as previously described (Conceicao C ...
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bioRxiv - Immunology 2022Quote: ... stimulated with SARS-CoV-2 spike protein overlapping peptide library or stimulated with 5μg/ml of Phytohaemagglutinin (PHA) (Sigma-Aldrich) as a positive control ...
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bioRxiv - Immunology 2021Quote: ADCP was performed using CHO cells stably expressing full-length wild-type SARS-CoV-2 S glycoprotein (target cells) fluorescently labelled with PKH67 Fluorescent Cell Linker Kits (Sigma Aldrich; Cat. Nr.: MINI67). Target cells were incubated with titrated concentrations of mAbs for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... 2×105 cells of each of transfected and untransfected cells stained with SARS-CoV-2 Spike antibody (SinoBiological) and Anti-human IgG FITC (Sigma-Aldrich®). After staining with primary and secondary antibodies ...
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bioRxiv - Molecular Biology 2021Quote: ... Anti-flavivirus group antigen antibody that probes ZIKV E protein and anti-SARS-CoV-1/2 NP 1C7C7 antibody was purchased from Sigma-Aldrich. Anti-influenza A virus nucleoprotein (NP ...
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bioRxiv - Microbiology 2020Quote: ... stained for VZV glycoprotein E (gE) (MAB8612, Millipore) in BD PermWash ...
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bioRxiv - Molecular Biology 2023Quote: Wild type and 2xSE variant of MpRBOHBN with an N-terminal 6 × His tag were expressed in Escherichia coli Rosetta-gami 2(DE3) (Novagen). Cells were grown at 37°C in lysogeny broth (LB ...
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bioRxiv - Immunology 2022Quote: SARS-COV-2 viral RNA was extracted using TRI Reagent (Sigma, USA). Reverse transcription was performed using HiScript II Q RT SuperMix for qPCR (Vazyme ...
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bioRxiv - Immunology 2020Quote: ... rabbit anti-SARS-CoV S polyclonal sera (BEI Resources) and mouse anti-2xStrep-tag antibody (Sigma-Aldrich). Anti-mouse IgG AF555 ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 S1 domain (ACRO biosystems – S1N-C52H3) or BSA (Sigma – A7906) overnight at 4°C in 50 μl/well ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNA sequence encoding 1053-1264 aa mouse was amplified and cloned upstream of sequences encoding a polyhistidine stretch (His) tag in pET23b (Novagen). The mGli2-His recombinant protein was expressed in BL21 (DE3 ...
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bioRxiv - Biochemistry 2023Quote: ... and expressed in BL21 Rosetta 2 (DE3) pLysS Escherichia coli (E. coli) cells (Novagen). All plasmids were verified by Sanger sequencing (OSU Shared Resources) ...
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bioRxiv - Molecular Biology 2020Quote: ... HIS-tag (Sigma-Aldrich H1029), and Streptactin-HRP (ThermoFisher Scientific #21130) ...
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bioRxiv - Microbiology 2023Quote: The His-tag system (Novagen) was used for Sif purification ...
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bioRxiv - Cell Biology 2022Quote: ... and SARS-CoV-2 was inactivated with 1% Triton-X (Sigma-Aldrich, Burlington, MA) in PBS for 1 hour ...
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bioRxiv - Microbiology 2021Quote: MERS-CoV and SARS-CoV-2 strains were obtained from different collaborators and passaged once in VeroE6 cells in DMEM (Sigma Aldrich) supplemented with 2% fetal bovine serum (Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2-infected cells were lysed in radio-immunoprecipitation assay (RIPA) buffer (Sigma-Aldrich) supplemented with 1% sodium dodecyl sulfate ...
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bioRxiv - Immunology 2023Quote: Recombinant SARS-CoV-2 BA.1 nucleocapsid protein was subcloned into the pET46 vector (Novagen) with an upstream 6xHis tag and expressed in Rosetta2 pLysS E ...
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bioRxiv - Biochemistry 2020Quote: ... FLAG tag (F1804) or His tag (H1029) were from Sigma. Mouse monoclonal antibody raised against hemagglutinin (HA ...
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bioRxiv - Neuroscience 2023Quote: ... His•Tag® (Millipore, 70796-3), anti-APP C-Terminal Fragment (BioLegend ...
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bioRxiv - Molecular Biology 2020Quote: ... cleaved His-tag and His-tag-fused TEV protease were removed using a HIS-Select® Nickel Affinity Gel (Sigma-Aldrich) column ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The SARS-CoV-2 nsp7 and nsp8 genes were codon optimized and cloned into pET46 (Novagen) with an N-terminal 6x histidine tag ...
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bioRxiv - Immunology 2021Quote: ... This SARS-CoV-2 pseudovirus was concentrated using an Amicon Ultra-15 filter (UFC910024, Sigma-Aldrich), and then stored at −80°C ...
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bioRxiv - Microbiology 2023Quote: ... of anti-spike murine antibodies (including cross-reactive mAbs against SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Microbiology 2024Quote: ... and -71 60 of anti-spike murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Cell Biology 2020Quote: ... anti-His tag (HIS.H8, 1:1,000, Millipore). The HRP-conjugated anti-mouse (111–035-144 ...
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bioRxiv - Immunology 2020Quote: ... cOmplete His-Tag Purification Resin (Sigma Millipore) was applied for affinity purification ...
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bioRxiv - Immunology 2020Quote: ... cOmplete His-Tag Purification Resin (Sigma Millipore) was applied for affinity purification ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant SIRV2 gp21 and SiL_0190 with C-terminal His-tag were expressed using Escherichia coli RosettaTM (DE3) cells (Novagen). Protein expression was induced during logarithmic growth through the addition of 0.5mM isopropyl-thiogalactopyranoside (IPTG ...
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bioRxiv - Microbiology 2020Quote: ... The flow-through from the His-tag cleavage containing the tag free protein was concentrated to 2 mL using Amicon Ultra-4 30k MWCO (Millipore). The concentrated protein was applied to a HiLoad 16/600 S75pg column (GE Life Sciences) ...
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bioRxiv - Biophysics 2023Quote: BCL-2 active humans (recombinant, expressed in E. coli) were purchased from Sigma. Navitoclax ...
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bioRxiv - Biochemistry 2023Quote: ... LPS (E. coli O111:B4, Sigma) was dissolved in PBS to make a stock solution then sonicated in a water bath for 10 min prior to addition to cell suspensions ...
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bioRxiv - Immunology 2020Quote: ... the SARS-CoV-2-RBD-binding phages were eluted with 100 mM triethylamine (Sigma-Aldrich, Xuhui, Shanghai). All eluted phages were collected and used to infect TG1 cells ...
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bioRxiv - Immunology 2021Quote: ... Eluted SARS-CoV-2 S containing fractions were concentrated using Amicon Ultra (cut-off: 30 KDa) (Millipore) and further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2022Quote: ... Each SARS-CoV-2 virus was isolated after three passages in Vero cells in DMEM (Sigma Aldrich). Virus isolation was confirmed by RT-PCR using E gene-specific primer sets (Extended Data Table 2) ...
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bioRxiv - Biochemistry 2022Quote: ... residual bound Coa and Efb were detected with anti-tag HRP-conjugated antibodies diluted 1:10000 in 2%BSA-PBST: α-HIS-tag antibody (A7058, Sigma) for EfbN ...
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bioRxiv - Immunology 2022Quote: PBMC or expanded T cell lines were stimulated for 5h at 37°C with or without SARS-CoV-2 peptides (2 μg/ml) in the presence of 10 μg/ml brefeldin A (Sigma-Aldrich). Cells were stained with the yellow LIVE/DEAD fixable dead cell stain kit (Invitrogen ...
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bioRxiv - Immunology 2020Quote: PBMC or Expanded T cell lines were stimulated for 5h at 37°C with or without SARS-CoV-1/2 peptide pools (2 μg/ml) in the presence of 10 μg/ml brefeldin A (Sigma-Aldrich). Cells were stained with the yellow LIVE/DEAD fixable dead cell stain kit (Invitrogen ...
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bioRxiv - Immunology 2020Quote: Expanded T cell lines were stimulated for 5h at 37°C with or without SARS-CoV-2 peptide pools (2 μg/ml) in the presence of 10 μg/ml brefeldin A (Sigma-Aldrich). Cells were stained with the yellow LIVE/DEAD fixable dead cell stain kit (Invitrogen ...