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Citations for Millipore Sigma :
301 - 350 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... coli (Novagen Rosetta 2 DE3, Sigma-Aldrich 71400) for protein expression ...
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bioRxiv - Cell Biology 2023Quote: ... coli Rosetta 2(DE3) cells (Millipore; 71400-4). E ...
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bioRxiv - Microbiology 2023Quote: ... coli strain Rosetta 2 (DE3) pLysS (EMD Millipore) cells were transformed with plasmids and grown overnight in LB plus appropriate antibiotics ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were transfected with pCG1-SARS-CoV-2_S plasmid using X-tremeGENE™ 9 DNA transfection reagent (Millipore-Sigma: 06365779001) (ratio 3:1 μl/μg DNA) ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were transfected with pCG1-SARS-CoV-2_S plasmid using X-tremeGENE™ 9 DNA transfection reagent (Millipore-Sigma: 06365779001) (ratio 3:1 μl/μg DNA) ...
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bioRxiv - Cell Biology 2019Quote: ... affinity purification for glycoproteins using Con A (Sigma) was performed overnight ...
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bioRxiv - Neuroscience 2021Quote: ... myelin associated glycoprotein (#MAB1567; MAG; Sigma; 1:500), and the CSPG brevican (#SC-135849 ...
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bioRxiv - Neuroscience 2023Quote: ... MOG (myelin oligodendrocyte glycoprotein, Cat#MAB5622, Merck Millipore) and NFH (neurofilament heavy chain ...
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bioRxiv - Molecular Biology 2021Quote: ... 1X EmbryoMax 2-mercaptoethanol (ES-007-E, Millipore Sigma), 10000 units/mL ESGRO Leukemia Inhibitory Factor (ESG1106 ...
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bioRxiv - Cell Biology 2023Quote: ... 0.15 mM 2-Mercaptoethanol (Millipore, Cat.No. ES-007-E), 100 U/ml Penicilline-Streptomycin (Gibco ...
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bioRxiv - Biochemistry 2020Quote: ... M1 activation was achieved with 100ng/mL lipopolysaccharide (LPS) treatment for 24h (E. coli; Sigma Aldrich), and M2 activation with 5ng/mL of murine interleukin-13 (IL-13 ...
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bioRxiv - Immunology 2021Quote: ... WT and Tet1−/− mice were intranasally injected with 0.5mg/kg LPS (E. coli 0111:B4, Sigma) for 4 hr.
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bioRxiv - Microbiology 2019Quote: ... EVs were incubated with Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) at different final concentration (0 to 10 µg/ml) ...
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bioRxiv - Immunology 2020Quote: ... with or without LPS (10 ng/ml from E. coli O111:B4, Sigma-Aldrich, Darmstadt, Germany). Cells were collected and stained with CD14 ...
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bioRxiv - Immunology 2021Quote: Transplanted mice were subjected to systemic LPS (from E. coli serotype O55:B5, Sigma, Rehovot, Israel) at 0.1 ...
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bioRxiv - Microbiology 2022Quote: ... cells were stimulated with 100 ng/ml LPS (lipopolysaccharides from E. coli O26:B6, Sigma-Aldrich) during the last 2 h of infection at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... before treatment with1µg/mL of Lipopolysaccharides (LPS; E. coli, serotype 055:B5, Sigma Aldrich cat#: L2637), for 1 hour.cat#: ...
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bioRxiv - Biophysics 2020Quote: ... and 0.3% v/v of N,N’-methylenebisacrylamide (bis-AA 2% w/v in DW, Sigma) in DI water for low ...
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bioRxiv - Molecular Biology 2023Quote: ... coverslips were incubated for 3 hours in 100 μl of 2% acrylamide (AA; A4058, Sigma-Aldrich) + 1.4% formaldehyde (FA ...
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bioRxiv - Microbiology 2021Quote: ... Dialysis was performed in concert with cleavage of the His-SUMO tag using the SUMO protease (Sigma Aldrich) at 20U/1mg of protein ...
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bioRxiv - Biochemistry 2020Quote: ... 2020) genes fused with an N-terminal His-tag sequence have been inserted into a pET28a vector (Novagen) for protein expression in E ...
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bioRxiv - Cell Biology 2023Quote: ... the 6×His tag on the proteins was further cleaved with Thrombin CleanCleave kit (Sigma-Aldrich, Cat# RECMT) overnight at 4 °C on the rocking table after desaltig in 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was flown through 500 μL of Roche cOmplete™ His-Tag purification resin (Millipore Sigma, #5893682001) for His-tag tagged proteins ...
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bioRxiv - Cell Biology 2020Quote: ... with 1% acrylamide (AA, 40%, Sigma) in PBS and incubated five hours at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: Antimycin A (AA, Sigma Aldrich A8674) was dissolved to make a stock solution of 5mM in dimethyl sulfoxide (DMSO ...
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: ... and 0.2mM ascorbic acid (AA; Sigma). For terminal differentiation/maturation ...
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bioRxiv - Physiology 2021Quote: ... arachidonic acid (AA; 0.05–0.5mM; Sigma), Horm collagen (0.1–10μg/ml ...
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bioRxiv - Biophysics 2022Quote: ... Acrylamide (AA, 40%, A4058, Sigma-Aldrich), Acrylamide/Bis-acrylamide (30% ...
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bioRxiv - Cell Biology 2023Quote: ... 10% acrylamide (AA, 40%, A4058, SIGMA) and 0.1% N,N’-methylenebisacylamide (BIS ...
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bioRxiv - Neuroscience 2023Quote: ... 200 μM AA (Sigma-Aldrich, A4544) and 100 μM cAMP (Biosynth ...
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bioRxiv - Neuroscience 2023Quote: ... 200 μM AA (Sigma-Aldrich, A4544) and 100 μM cAMP (Biosynth ...
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bioRxiv - Neuroscience 2023Quote: ... 200 μM AA (Sigma-Aldrich, A4544) and 100 μM cAMP (Biosynth ...
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bioRxiv - Cell Biology 2023Quote: ... Sigma)/8% acrylamide (AA, 40%, A4058, Sigma) diluted in PBS was added to achieve a final concentration of 4%FA/4%AA ...
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bioRxiv - Molecular Biology 2021Quote: ... M2 anti-FLAG tag (1:1000, from 1 mg mL−1, Sigma), anti-β-actin (1:5000 ...
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bioRxiv - Cell Biology 2023Quote: ... M2 anti-FLAG tag (1:1000, from 1 mg mL−1, Sigma), anti-β-actin (1:5000 ...
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bioRxiv - Microbiology 2023Quote: ... a BiP secretion sequence and a C-terminal C-tag) was expressed from a stable S2 cell line34 (ExpreS2ion Biotechnologies) in EX-CELL® 420 Serum Free Medium (Sigma Aldrich). After 3-4 days ...
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bioRxiv - Cancer Biology 2023Quote: ... 2% heat-inactivated Fetal Bovine Serum (HI-FBS; Sigma-Aldrich), 1% v/v penicillin-Streptomycin (VWR) ...
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bioRxiv - Cell Biology 2023Quote: ... and the His-labelled prey proteins were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen-Millipore, 70954) and incubated in liquid cultures ...
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bioRxiv - Cell Biology 2023Quote: ... and the His-labelled prey proteins were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen-Millipore, 70954) and incubated in liquid cultures ...
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bioRxiv - Immunology 2022Quote: ... of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Immunology 2021Quote: ... or 900-fold (SARS-CoV-1) diluted plasma samples before addition of THP-1 cells (20,000 cells per well; Millipore Sigma, Burlington, MA). After 19 h incubation at 37°C ...
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bioRxiv - Biochemistry 2019Quote: ... rabbit monoclonal DYKDDDDK tag (Flag tag, 1:1000, CST, #14793) and/or mouse monoclonal α-tubulin (1:10,000, Millipore, 05-829). Following three washes with TBS-T ...
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bioRxiv - Neuroscience 2022Quote: ... mitoProfile total OXPHOS Human WB antibody cocktail (1:1000, ab110411), SDHA monoclonal antibody (1:10000, 459200), anti-TOMM20 (1:1000, HPA011562) and actin antibody (1:1200, Sigma A2066). Four 5 min washes with PBS-T (0.005% tween ...
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bioRxiv - Cell Biology 2022Quote: ... Next day the coverslips were treated with Acrylamide (AA)-Formaldehyde (FA) Solution [1.4% AA (Sigma Aldrich: A4058) and 2% FA (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... coli BL21 Rosetta 2 (DE3) pLysS strain (Novagen, #70956). The protein expression was induced with 0.5 mM IPTG and followed by culturing at 20 °C for 16 h ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were expressed in Escherichia coli Rosetta 2 (Novagen) in LB media ...
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bioRxiv - Immunology 2020Quote: ... or treated with lipopolysaccharide (LPS) (10 ng/ml from E. coli O111:B4, Sigma-Aldrich, Darmstadt, Germany), and 10 μg/ml Pam3Cys (P3C ...
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bioRxiv - Immunology 2020Quote: ... The cells were left unstimulated or stimulated with 100 ng/mL LPS (E. coli 0111:B4; Sigma) for 4h (for qPCR ...
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bioRxiv - Microbiology 2023Quote: ... 5% CO2. Cells were stimulated by adding 500 ng/mL LPS (E. coli 0111:B4) (Sigma, USA) into the medium specified above ...
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bioRxiv - Neuroscience 2023Quote: Mice were given single intraperitoneal (i.p.) injections of lipopolysaccharide (LPS; E coli, O111:B4; Sigma, St Louis) (250μg/kg in 2mL/kg ...