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Citations for Millipore Sigma :
501 - 550 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Cells were stimulated for 4 h with one of the following: LPS (E. coli 0127:B8 100 ng/ml; Sigma-Aldrich); solutions of mix of synthetic neuromelanins (NM 10 μg/mL of Pheo-β-LG and 10 μg/mL of Pheo-β-fl) ...
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bioRxiv - Neuroscience 2022Quote: ... media was aspirated and cells were exposed to 10 or 50 μg/mL lipopolysaccharide (LPS) (E. coli strain 0111:B4; Sigma-Aldrich) (MilliQ H2O vehicle ...
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bioRxiv - Systems Biology 2023Quote: ... All E. coli strains (a gift from Dr. Christian Rudolph) were routinely grown in modified Luria Broth (LB) (1% tryptone (Sigma-Aldrich), 0.5% yeast extract (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... either fresh or thawed DCs (differentiation day 8) were stimulated for 24 h with 200 ng/ml lipopolysaccharide (LPS; E. coli O26:B6, Sigma-Aldrich) and used for experiments on day 9 ...
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bioRxiv - Immunology 2023Quote: ... CD11c+ were purified and either plated with no further supplementation or stimulated with 100 ng/mL or 1 μg/mL of lipopolysaccharide (LPS, E. coli O111:B4, Sigma-Aldrich).
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bioRxiv - Neuroscience 2023Quote: ... 8-week old male and female Cx3cr1-GFP mice bred on a C57BL/6J background (n=2 females and 1 male/condition) were intraperitoneally injected with 0.5 mg/kg lipopolysaccharide (LPS; Lipopolysaccharides from E. coli O55:B5, Sigma-Aldrich L5418) or vehicle solution (PBS ...
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bioRxiv - Neuroscience 2023Quote: ... 8-week old male and female C57BL/6J mice (n=2/sex/condition) were intraperitoneally injected once with 1.0 mg/kg lipopolysaccharide (LPS; Lipopolysaccharides from E. coli O55:B5, Sigma-Aldrich L5418) or vehicle solution 1xPBS (Phosphate buffered saline ...
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bioRxiv - Genetics 2023Quote: ... each probe set was added (final concentration of 500 nM) to 20mM VRC and hybridization buffer (1% Dextran sulfate [EMD Millipore], 1 mg/mL E. coli tRNA [Sigma] ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 μM mVP40 proteins or His-tag alone were incubated with 50 μg/ml Ni-NTA-Atto 550 dye (Millipore Sigma, Burlington, MA) in a final volume of 500 μl ...
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bioRxiv - Systems Biology 2021Quote: ... The N-terminal His-tag was digested at room temperature overnight using up to 12 NIH Units per mL of bovine thrombin (Sigma-Aldrich T4648-10KU). Proteins were then purified using size exclusion chromatography (HiLoad 26/600 Superdex 200 pg column from GE Healthcare) ...
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bioRxiv - Plant Biology 2022Quote: ... in the same buffer containing 1/5000 of primary antibody raised against poly-His tag directly coupled with peroxidase (Sigma, catalog no. A7058). After washing 3x by PBS 1x ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and 1 mg/ml AAG (human a1-acid glycoprotein, Sigma, Cat # G9885). All medium is sterilized by filtration through a 0.22mm membrane ...
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bioRxiv - Cell Biology 2020Quote: ... anti-SOD-2 (1:1000, Sigma, SAB2701618); anti-phospho-AKT ...
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bioRxiv - Cell Biology 2020Quote: ... Prohibitin 2 (Sigma Aldrich, HPA039874, 1:1000), OCIAD2 (Rabbit serum ...
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bioRxiv - Molecular Biology 2019Quote: ... His-GFP or His-fusion proteins were bound to HIS-Selec HF Nickel Affinity Gel (Sigma) for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... strep tag and myc tag were obtained from Sigma, IBA and Abcam ...
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bioRxiv - Immunology 2022Quote: ... Non-specific binding sites were blocked using fluorescence-activated cell sorting (FACS) buffer (1× PBS, 2% hi-FBS) supplemented with 20% hi-FBS and 10 μg mouse IgG (Sigma-Aldrich, St-Louis, MO, USA). Cells were stained using the following conjugated mouse anti-human monoclonal antibodies (mAbs) ...
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bioRxiv - Neuroscience 2021Quote: ... treated with poly-d-lysine(Sigma-Aldrich A-003-E, 1-2 hr at 37°C, 2 μL/well) followed by laminin (Thermo Fisher 23017015 ...
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bioRxiv - Bioengineering 2019Quote: ... coli in 2% alginate solution supplemented with 2% Luria-Bertani (LB) broth (Sigma Aldrich, St. Louis, MO, USA). For ink preparation ...
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bioRxiv - Systems Biology 2022Quote: ... or 50 mM acrylamide (AA) (Merck Sigma, Cat. No. A9099), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... starvation medium was replaced with aa containing medium (D6429 Sigma) for the indicated time ...
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bioRxiv - Developmental Biology 2020Quote: ... Injected blastocysts were cultured in KSOM/AA (Millipore, Billerica, MA) at 37ºC in an atmosphere of 5% CO2 to allow for recovery of blastocyst morphology and then implanted into the uterine horns (up to ten embryos per horn ...
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bioRxiv - Genomics 2022Quote: ... 1X Non-Essential AA (Millipore, Cat. No. TMS- 001-C), 0.15 mM 2-Mercaptoethanol (Millipore ...
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bioRxiv - Genomics 2022Quote: ... 1X Non-Essential AA (Millipore, Cat. No. TMS-001-C), 0.15 mM 2-Mercaptoethanol (Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... the AAs were functionalized with poly-D-ornithine (Sigma-Aldrich) (50 µg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... 1% AA and 2mM L-Glutamine (Sigma, Cat. no. 59202C). The cell line UPCI-SCC-154 was purchased from the German Collection of Microorganisms and Cell Cultures GmbH (DMSZ ...
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bioRxiv - Molecular Biology 2020Quote: ... melanogaster (fly) Schneider 2 (S2) cells were grown in 12 ml Schneider’s insect medium (Sigma-Aldrich, cat. no. S0146) containing 10% (v/v ...
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bioRxiv - Developmental Biology 2021Quote: ... total RNA was isolated from young adult fruit flies (2-5 days) or S2 cells using TRI Reagent (Sigma) according to the manufacturer specifications ...
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bioRxiv - Synthetic Biology 2021Quote: ... rhaeticus cultures were grown shaking to mid exponential phase. Cells treated with 50 nM (E. coli) or 50 μM (K. rhaeticus) of AHL (N-Acyl homoserine lactone, Sigma-Aldridge K3255) and induced for approximately 4 doubling times (i.e ...
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bioRxiv - Cancer Biology 2022Quote: ... The S2-013 and S2-LM7AA lines were transduced with lentivirus encoding either shRNA against ST6GAL1 (Sigma, cat#TRCN00000035432) or a control shRNA (Sigma ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli cell-free TXTL extract was prepared from BL21 Rosetta 2 from Novagen, as described previously.23,24 The TXTL system is commercially available as the product “myTXTL” from Arbor Biosciences ...
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bioRxiv - Immunology 2020Quote: ... coli lipopolysaccharides (LPS, 0.08, 0.4, and 2 μg/ml; L2630-10MG, Sigma, USA), S ...
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bioRxiv - Plant Biology 2022Quote: ... coli strains derived from BL21(DE3) and Rosetta 2 (Merck Millipore, Burlington, USA), BL21(DE3)-R3-pRARE2 and BL21(DE3)-R3-lambda-PPase ...
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bioRxiv - Biophysics 2023Quote: ... recombinant Rad26 protein was expressed in Escherichia coli strain Rosetta 2(DE3) (Novagen) and purified by Ni-NTA agarose (Qiagen) ...
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bioRxiv - Bioengineering 2022Quote: ... Sections were stained with H&E and 2% Alizarin Red solution (all Sigma-Aldrich).
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bioRxiv - Cell Biology 2019Quote: ... α-His (Sigma H1029). α-Rabbit IgG HRP conjugate (Promega W4011) ...
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bioRxiv - Biochemistry 2020Quote: ... PCNA-12 co-expression plasmids were constructed by inserting non-tagged PCNA-1 into multiple cloning site 1 and PCNA-2 (without and with a His6-tag) into site 2 of pET-DUET-1 plasmid (Novagen).
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bioRxiv - Microbiology 2020Quote: ... Anti-ICP4 MAb H1A021 or monoclonal Ab to VSV glycoprotein G (P5D4, Sigma) was added for 1 h followed by Alexa Fluor 488-labeled goat anti-mouse IgG (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... Then 1 µg/well of biotinylated α1-acid glycoprotein (Sigma, Catalogue number-112150) or 3’-sialyllactose-PAA-biotinylated (Glycotech ...
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bioRxiv - Developmental Biology 2023Quote: ... DAPI (1:1200, Sigma-Aldrich, #D9564).
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bioRxiv - Biophysics 2022Quote: ... For E-Cadherin stainings a 1:1000 dilution of Alexa 647-conjugated anti-rat (Sigma-Aldrich SAB4600186) was used ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μg of purified His fusion protein (PuBZR1-His) was bound to Ni-NTA His binding resin (Novagen). GST fusion proteins containing PuACO1 (PuACO1-GST ...
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bioRxiv - Biochemistry 2020Quote: ... His-PBP1B containing fractions were pooled and treated with 2 U/mL of thrombin (Novagen) for 20 h at 4 °C during dialysis against dialysis buffer I (25 mM Tris-HCl ...
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bioRxiv - Cell Biology 2024Quote: ... containing HI-2 at final concentration of 100 µM (or vehicle control DMSO, Sigma-Aldrich) was added to the cells ...
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bioRxiv - Plant Biology 2022Quote: ... Various recombinant TFIIIA-7ZF proteins with an intein-chitin binding domain (CBD) tag were overexpressed using the Escherichia coli BL21(DE3) Rosetta strain (EMD Millipore, Burlington, MA). For each construct ...
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bioRxiv - Cell Biology 2021Quote: ... Flag-tag (Sigma), Myc-tag (Upstate) ...
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bioRxiv - Neuroscience 2022Quote: ... MAP-2 (rabbit, Millipore, cat# AB5622, 1: 1000), Rabbit monoclonal anti-S100β (Abcam ...
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bioRxiv - Biophysics 2022Quote: ... the N-terminal hexa-histidine tag was cleaved with ∼2 Units of bovine thrombin (Sigma) per 1 mg of protein ...
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bioRxiv - Microbiology 2021Quote: ... S2 or S2 P-mutant subunits in the presence of increasing doses (0.25-1 ng/ml) of LPS (Sigma, USA). Then ...
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bioRxiv - Microbiology 2022Quote: ... SasG-His was then re-purified using HIS-Select resin (Sigma) and eluted with bind buffer containing increasing concentrations of imidazole ...