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Citations for Millipore Sigma :
301 - 350 of 10000+ citations for SARS CoV 2 Nucleoprotein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: Full length human KBP residues 1-621 in a PSTCm1 expression vector (with kanamycin resistance and a N-terminal thrombin cleavable 6 x His-tag) was expressed in Rosetta2 cells (Novagen). Following immobilised metal-affinity chromatography with Ni-NTA resin (Qiagen) ...
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bioRxiv - Evolutionary Biology 2020Quote: Proteins were expressed in a pET28/30 vector containing an N-terminal His tag with a TEV protease cleavage site (Millipore). For each protein ...
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bioRxiv - Cell Biology 2020Quote: ... the α-PheRS or α-PheRSCys mutant cDNAs were cloned with His tags at the N-terminal end into the pET-28a plasmid expression vector (Novagen). Wild-type β-PheRS cDNAs were cloned into the pET LIC (2A-T ...
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bioRxiv - Biophysics 2021Quote: The bacteriophage T7 gp16 gene was cloned into the pET-16b vector (ampr and 10x-His tag) from Novagen (Germany), inserted between NdeI and XhoI restriction sites ...
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bioRxiv - Biochemistry 2021Quote: SUMO-peptide fusions were cloned into a pDW363 vector that appends a BAP sequence and 6x-His tag to the N-terminus of the protein and transformed into Rosetta2(DE3) cells (Novagen). Rosetta2(DE3 ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNA sequence encoding 1053-1264 aa mouse was amplified and cloned upstream of sequences encoding a polyhistidine stretch (His) tag in pET23b (Novagen). The mGli2-His recombinant protein was expressed in BL21 (DE3 ...
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bioRxiv - Plant Biology 2020Quote: ... the coding sequence was excised with BamHI/EcoRI and ligated downstream of the His tag into the multiple cloning site MCS1 of pETDuet-1 (ampicillin resistance; Novagen), yielding Sll0171-pETDuet-1 ...
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bioRxiv - Bioengineering 2022Quote: ... It was cloned in the expression vector pET30 Ek/lic containing an N-terminal His-tag with the help of the ligation independent Ek/lic system according to the protocol proposed by Novagen, UK ...
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bioRxiv - Immunology 2020Quote: ... The elimination of the N-terminal His tag was performed through the cleavage with biotinylated thrombin included in the Thrombin Cleavage Capture Kit (Merck, Millipore) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... was cloned into the protein expression vector pET28a (+) containing an IPTG-inducible promoter and a C-terminal 6x-His tag (Novagen). Expression of CosR-His was then induced in E ...
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bioRxiv - Microbiology 2019Quote: ... and the PCR product was purified and cloned in-frame with a His-tag into the IPTG-inducible expression plasmid pET-15b (Novagen) to create construct pET15b-Pnf ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Mouse anti-FLAG M2 monoclonal antibody (F1804) and mouse anti-His-tag monoclonal antibody (D291-3S) were purchased from Sigma□Aldrich (St ...
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bioRxiv - Biochemistry 2023Quote: The NP gene from strain A/WSN/33 (H1N1) with a His-tag at its C-terminal was cloned in the pET22 plasmid (Novagen). Single point mutation R416A was introduced through the Quick-Change kit (Stratagene ...
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bioRxiv - Synthetic Biology 2022Quote: ... Grids were blocked in 2% BSA in TBST for 30 minutes and then moved into a 20 µl drop of anti-His tag primary antibody (Sigma) diluted 1:100 ...
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bioRxiv - Biochemistry 2023Quote: Expression of myrArf1 proteins with C-terminal 6x His tags was accomplished via co-expression with yeast N-myristoyltransferase (yNMT) using the pETDuet-1 vector (Novagen) in E ...
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bioRxiv - Biochemistry 2019Quote: ... coli strain Rosetta 2 (DE3) pLysS (EMD Millipore, Billerica MA). Cultures were grown at 37°C to A600=0.5 ...
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bioRxiv - Neuroscience 2021Quote: ... coli cells Rosetta 2 (DE3) Competent Cells (Novagen, Sigma-Aldrich). Arc S67A and T278A DNA was amplified from point-mutant pCMV6-Arc-Myc-FLAG plasmids prepared using site-directed mutagenesis and subcloned similarly ...
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bioRxiv - Neuroscience 2021Quote: ... coli cells Rosetta 2 (DE3) Competent Cells (Novagen, Sigma-Aldrich). Arc S67A and T278A DNA was amplified from point-mutant pCMV6-Arc-Myc-FLAG plasmids prepared using site-directed mutagenesis and subcloned similarly ...
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bioRxiv - Microbiology 2021Quote: ... coli JM109 cells transformed with a pETBlue-2 plasmid (Novagen) containing the gene for alcohol dehydrogenase (ADH ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... coli Rosetta™ 2(DE3) pLysS cells (Millipore Sigma, USA). The cells were grown in the 2x YT media containing 100 μg/mL of ampicillin and 50 μg/mL of chloramphenicol at 37 °C until O.D ...
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bioRxiv - Neuroscience 2022Quote: ... coli Rosetta 2 competent cells (Novagen, San Diego, CA, USA) and cultured in the medium ...
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bioRxiv - Molecular Biology 2024Quote: ... was transformed into Escherichia coli strain Rosetta 2 pLys (Novagen), followed by culturing in 2xYT and kanamycin (50 μg/ml ...
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bioRxiv - Immunology 2022Quote: ... of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Microbiology 2022Quote: ... and −7173 of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Microbiology 2022Quote: ... of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Microbiology 2023Quote: ... of anti-spike murine antibodies (including cross-reactive mAbs against SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Microbiology 2023Quote: ... and -71)60 of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Microbiology 2024Quote: ... and -71 60 of anti-spike murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Developmental Biology 2024Quote: ... H&E staining was performed with Gills Type 2 Hematoxylin (Sigma) and 1:1 Eosin (Fisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: The genes of either E protein or E DIII protein (residues 301-403) of ZIKV (GZ01, KU820898) without tag were cloned into pET28a vectors (Novagen) and expressed by IPTG-induction in BL21 (RIL ...
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bioRxiv - Immunology 2021Quote: ... The same volumes of PBS and LPS (20 μg/mouse, from E. coli O114:B4, Sigma-Aldrich, Shanghai, China) were used as negative control and positive control ...
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bioRxiv - Immunology 2020Quote: ... Cells were plated in 96-well plate in density of 1×106/mL in 200μL of medium and cultured at 37°C and 5% CO2 for 48h in presence of 5μg of lipopolysaccharide (LPS, isolated from E. coli strain 055:B5, Sigma, St ...
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bioRxiv - Immunology 2019Quote: ... 30 minutes before being challenged with 40 mg kg−1 LPS (E. coli, serotype 0111:B4, L-2630, Sigma). Mice were euthanized 3 hours after LPS challenge for blood collection ...
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bioRxiv - Neuroscience 2023Quote: ... 13 weeks old mice were injected intraperitoneally (i.p.) with 5□mg/kg of LPS (L2880, E. coli O55:B5, Sigma) or endotoxin-free PBS between 1 p.m ...
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bioRxiv - Bioengineering 2024Quote: ... were injected intraperitoneally (i.p.) with PBS or 10 mg/kg of lipopolysaccharide (LPS from E. coli O111:B4, 1 µM; #L2630, Sigma). After 8 h ...
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bioRxiv - Microbiology 2020Quote: ... 1:2000 (SARS-1-S, MERS-S and SARS-2-S) or 1:5000 (229E-S)] and mouse anti-β-actin (Sigma-Aldrich, A5447, 1:5000). As secondary antibody ...
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bioRxiv - Biochemistry 2020Quote: ... N-terminally His-tagged MpPA14 was incubated with the array and binding of the lectin to the polysaccharides was detected by an anti-His tag secondary antibody conjugated to alkaline phosphatase (Sigma-Aldrich). Microarray probing and quantification were performed as previously described(41).
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bioRxiv - Molecular Biology 2021Quote: ... un-spliced precursor and mutant SufB proteins carrying an N-terminal 6X-His-tag were over-expressed in BL21 (DE3) E.coli cells via IPTG (500µM) (Sigma 367-93-1) induction at 37°C for 4hrs ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was nutated with 200 μL of Ni-NTA resin (Roche cOmplete™ His-Tag purification resin, Millipore Sigma #5893682001) at 4°C for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... and then monoclonal anti-6x(HIS)-tag antibody was probed followed by detection with anti-mouse IgG conjugated with alkaline phosphatase (A3562: 1ml Sigma, USA) was used for developing the blots.
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bioRxiv - Cell Biology 2024Quote: ... respectively (Millipore Cat# HI-14K, HI-13K). Samples treated with 3 mM glucose KRBH were measured undiluted ...
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bioRxiv - Microbiology 2020Quote: ... cells were permeabilized and incubated with FITC-labeled mouse anti-RSV Nucleoprotein (Millipore), followed by washes with PBS containing 1% BSA ...
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bioRxiv - Biochemistry 2023Quote: Escherichia coli Origami 2 (DE3) (Millipore Sigma, St. Louis, MO, USA) were transformed with pET-28a(+ ...
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bioRxiv - Cell Biology 2019Quote: ... carrying two isoleucine residues at the N-terminus and a hexahistidine tag at the C-terminus was expressed in Escherichia coli Rosetta2(DE3)pLysS cells (Novagen) and purified by immobilized metal-affinity chromatography followed by gel-filtration chromatography as described previously (Bishop et al. ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μM (2’Z,3’E)-6-Bromoindirubin-3’-oxime (Sigma, B1686), 20 μM Tideglusib (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Acute inflammatory-type macrophages were induced by treating cells with 10 ng/mL lipopolysaccharide (from E. coli 055:B5, Sigma) for 3.5 hours ...
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bioRxiv - Neuroscience 2020Quote: ... vehicle (HBSS) or 100 ng/mL lipopolysaccharide (LPS E. coli O111:B4; 500,000 EU/mg; Sigma-Aldrich; St. Louis, MO) was administered for 3 hrs in Hx or Nx ...
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bioRxiv - Biophysics 2022Quote: ... coli optimized gene sequence for expression of S.c. Cdc6 in E. coli (a generous gift of Dr. Megan Davey, Western University, Canada) was cloned into pET11 (Novagen). The Cdc6 gene was then subcloned into pGEX-6P-1 (GeneScript ...
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bioRxiv - Neuroscience 2019Quote: hNSG and non-engrafted NSG mice (4-6 months old) were injected 3 mg/kg (i.p.) with lipopolysaccharide (LPS from E. coli; O55:B5, Sigma-Aldrich) or 0.9% saline solution ...
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bioRxiv - Molecular Biology 2020Quote: ... and calmodulin was ordered from Genewiz (codon optimized for expression in E. coli) and cloned into the NcoI and EcoRI sites of pET-28a (Novagen).