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Citations for Millipore Sigma :
551 - 600 of 10000+ citations for SARS CoV 2 Nucleoprotein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... coli were also grown in 2 mL Overnight Express Instant TB media (Sigma Aldrich, 71491-5) with added 100 μg/mL ampicillin ...
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bioRxiv - Plant Biology 2023Quote: ... Individual expression constructs were subsequently transformed into Escherichia coli Rosetta™ 2 (DE3)pLysS cells (Novagen) for heterologous expression ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed then sequentially incubated with anti-SARS-CoV-2 CR3022 antibody (Yuan et al., 2020) (1 μg/mL) and a HRP-conjugated goat anti-human IgG (Sigma) in PBS supplemented with 0.1% (w/v ...
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bioRxiv - Genomics 2022Quote: ... Infectious foci were stained with a human anti-SARS-CoV-2 Spike antibody (H2-162, Hugo Mouquet’s laboratory, Institut Pasteur) and the corresponding HRP-conjugated secondary antibody (Sigma-Aldrich). Foci were visualized by 3,3’-diaminobenzidine staining solution (DAB ...
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bioRxiv - Microbiology 2019Quote: ... anti-His (1:1000 Sigma), or anti-StrepII (1:3000 IBA ...
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bioRxiv - Cancer Biology 2019Quote: ... rabbit anti-His (Sigma Aldrich), rabbit anti-Tubulin polyclonal antibody (Cell Signaling Technology).
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bioRxiv - Cancer Biology 2020Quote: ... The pETDuet-1-His-BAP1-ULD+ASXL2-AB protein complex was expressed in Rosetta 2 (DE3) pLysS (Millipore). The bacteria bearing the desired plasmids were propagated with aeration at 37°C in 1L of 2YT to an A600 absorbance of approximately 0.6 ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant was isolated and combined with 2 ml of nickel-nitrilotriacetic acid (Ni-NTA) His Bind resin (Novagen), equilibrated in lysis buffer and incubated for 1 hour at 4°C with gentle rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 mM β-mercaptoethanol prior to application of the protein to His-Select Cobalt affinity gel (Sigma-Aldrich). The slurry was rotated for 60 min prior to application to a gravity flow column ...
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bioRxiv - Biochemistry 2023Quote: ... or a VSVG epitope tag on the N-terminal tag of H2A (Sigma, V4888) and a fluorescently labelled secondary antibody (Cell Signaling ...
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bioRxiv - Cancer Biology 2021Quote: ... Flag-tag (Sigma, F3165-.2MG), Veriblot IgG (Abcam ...
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bioRxiv - Genomics 2019Quote: ... Flag-tag M2 (F1804, Sigma), goat IgG control (sc-2028 ...
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bioRxiv - Microbiology 2019Quote: ... Flag-tag (Sigma-Aldrich, 1;1000) and β-actin (Sigma-Aldrich ...
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bioRxiv - Genetics 2022Quote: ... Anti-FLAG tag (Sigma, #F1365), anti-PGK1 (Thermo # PA5-28612) ...
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bioRxiv - Cell Biology 2021Quote: ... S tag (EMD Millipore; MAC112), IRDye 680RD Streptavidin (LI-COR ...
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bioRxiv - Cell Biology 2022Quote: ... anti-S-tag from Novagen, anti-V5 from GenScript and anti-GFP from Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2022Quote: ... HA-Tag (Sigma H3663-200UL), ACF1 (Abcam ab187670) ...
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bioRxiv - Biochemistry 2023Quote: ... and FLAG tag (Sigma, F3165).
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bioRxiv - Neuroscience 2021Quote: ... 3 month-old WT (C57BL/6J) and TSPO-KO mice were injected with phosphate buffered saline (PBS, control) or LPS (Lipopolysaccharide from E. coli, Sigma Aldrich; 1 mg/kg body weight i.p.) daily for four consecutive days.
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bioRxiv - Microbiology 2021Quote: ... E2 (CHIK-102 and CHIK-263) [42] or Zika Virus E as a control (ZV-2, Sigma-Aldrich) (kindly provided by Dr ...
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bioRxiv - Bioengineering 2023Quote: dPPM was stained with H&E (Hematoxylin solution, Gill no.2, Sigma Aldrich, aqueous Eosin Y, Thermo scientific) to assess cellular content and histoarchitecture ...
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bioRxiv - Biophysics 2021Quote: ... and the e-cadherin with monoclonal rat anti-e-cadherin (Sigma, catalogue no. U3254) primary antibody followed by polyclonal anti-rat IgG secondary antibody conjugated CF-647 (Sigma ...
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bioRxiv - Immunology 2022Quote: ... CHO CoV-2-Spike cells were used as target cells and were fluorescently labeled with PKH67 Fluorescent Cell Linker Kit (Sigma Aldrich) prior to incubation with mAbs ...
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bioRxiv - Cell Biology 2021Quote: ... The Bin1b-SH3 construct was transformed into Eschericia coli RosettaTM 2 (DE3) competent cells (Novagen, Merck, 71403) by heat shock ...
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bioRxiv - Bioengineering 2020Quote: ... we cultured Escherichia coli strain JM109 in 250-mL 2× Yeast extract Tryptone growth medium (Sigma Aldrich) with 5 mM MgCl2 (Sigma Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... coli polar lipids (Avanti, US) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) (Sigma Aldrich) in a 3:1 molar ratio ...
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bioRxiv - Immunology 2022Quote: ... containing 2% (vol/vol) of heat inactivated (HI, for 1h at 56°C) human AB serum (hABS) (Sigma, #H3667). After extensive washings with DPBS (Gibco ...
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bioRxiv - Immunology 2021Quote: Expression cassette of maltose binding protein (MBP)-Mb2Cas12a-His in pMal vector was transformed to Rosetta 2 (DE3) (Novagen) competent cells ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was isolated and combined with 2 ml of nickel-nitrilotriacetic acid (Ni-NTA) His Bind resin (Novagen), incubated at room temperature for 20mins and poured into a 1-cm separation column (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... E-64 (Sigma-Aldrich, E3132), fosmidomycin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... 0.1 μM Compound E (Millipore), 2.5 μM DAPT (Sigma Aldrich) ...
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bioRxiv - Plant Biology 2019Quote: ... E-64 (Sigma, 10 µM) were dissolved in H2O ...
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bioRxiv - Microbiology 2020Quote: ... then Pronase E (Sigma-Aldrich) at 20 ug/ul for 30 min at 37 oC ...
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bioRxiv - Molecular Biology 2020Quote: ... 1X E SGRO LIF (Millipore), 5 nM GSK-3 inhibitor XV and 500 nM UO126 ...
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bioRxiv - Neuroscience 2022Quote: ... 0.1 μM Compound E (Millipore), 2.5 μM DAPT (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2020Quote: ... Compound E (EMD Millipore 565790) was used at a final concentration of 10μM for 18h for the γ-secretase inhibited samples ...
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bioRxiv - Cancer Biology 2022Quote: ... Vitamin E (125μM, Sigma Aldrich), Cyclosporin A (1μM ...
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bioRxiv - Cancer Biology 2022Quote: ... 20 μM E-64 (Sigma), 0.7 μM Aprotinin (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... E-64d (Sigma Aldrich, #E8640), Pepstatin A (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... E-64d (Sigma, cat# SML0057), SB412515 (Cayman Chemical ...
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bioRxiv - Molecular Biology 2024Quote: ... E-64 (Sigma-Aldrich; #324890), doxycycline (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... Viral plaques were stained with anti-LCMV nucleoprotein (anti-LCMV NP, clone VL4) and HRP conjugated anti-rat IgG (Millipore) and visualized with O-phenylenediamine (OPD ...
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bioRxiv - Immunology 2024Quote: ... the plates were washed three times and a solution of anti-nucleoprotein antibodies (Sigma, #MAB8257 and #MAB8258, 1:4000 each) in antibody diluent were added for 1 hour at room temperature ...
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bioRxiv - Biophysics 2019Quote: ... coli Rosetta2 (DE3) (Novagen). For primary culture ...
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bioRxiv - Molecular Biology 2020Quote: ... coli BL21 (DE3) (Novagen) was transformed by the plasmids ...
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bioRxiv - Cell Biology 2020Quote: ... coli tRNA (Sigma-Aldrich), dried with a speed-vac ...
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bioRxiv - Molecular Biology 2021Quote: ... coli (Sigma Aldrich, P5931) in 10 mM ammonium acetate ...
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bioRxiv - Microbiology 2019Quote: ... coli DH5α cells (Novagen) for DNA production and purification ...
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bioRxiv - Immunology 2019Quote: ... Coli Lipopolysaccharide (Sigma-Aldrich) at 100ng/mL ...
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bioRxiv - Biochemistry 2019Quote: ... coli tRNA (Sigma R1753) was titrated into 20 μM PSHCP (either PSHCP1-62 or PSHCP1-56 ...