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Citations for Millipore Sigma :
601 - 650 of 10000+ citations for Rh Family C Glycoprotein RHCG Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The proteins were eluted using 250 µg/mL Flag peptide into wash buffer for 1 h at 4°C and subjected to immunoblotting analysis using antibodies for Flag tag (SIGMA, SLCD6338) and SARS-CoV-2 S (Sino Biological ...
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bioRxiv - Neuroscience 2020Quote: ... followed by overnight incubation at 4 °C with the following primary antibodies diluted in blocking solution: mouse anti-GFAP (1:500, Sigma G3893), guinea pig anti-NeuN (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 48 hours at 4°C with the following primary antibodies: anti-GBA (1:500; human 8E4; human and rodent G4171, Sigma-Aldrich), anti-human α-synuclein (syn211 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in primary antibody diluted in blocking solution (PBST, 1% BSA) overnight at 4°C with the following primary antibodies: mouse anti-NeuN (1:500, Millipore, Mab377), rat anti-BCL11B (1:1,000 ...
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bioRxiv - Biochemistry 2020Quote: ... with purified antigens and used at a dilution of 1:5,000 in TBS-Blotto with 0.05% vol/vol Tween20 (Pilonieta et al., 2007; Rivas et al., 2020) Primary antibodies against c-Myc (Sigma-Aldrich M4439) and DnaK (AbCam ab69617 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked with 5% bovine serum albumin in tris-buffered saline/0.1% [vol/vol] tween-20 (TBS-T) before probing overnight at 4°C with antibodies raised to the following targets: mouse anti-puromycin (Millipore-Sigma, MABE343), mouse anti-OC43 N (CoV antibody ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with primary antibodies in blocking buffer for 1h at 21°C: 1:1000 α-tubulin (118M4779V; Sigma Aldrich), 1:250 CREST (15-234-0001 ...
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bioRxiv - Cancer Biology 2019Quote: ... overnight at 4°C and followed by 1-h incubation at room temperature with HRP-conjugated anti-rabbit secondary antibody (1:2000, Sigma Aldrich). Chemiluminescence was detected using ECL Western Blotting substrate (Merck ...
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bioRxiv - Cancer Biology 2019Quote: ... Membranes were incubated with primary antibodies overnight at 4°C (rabbit anti-ORF2 mAbs at 1:1000; mouse anti-Flag M2 (Sigma F1804) at 1:2000) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... then blocked with 4% NHS/TBST-0.2% and incubated overnight at 4°C in primary antibody solution (1:2500 anti-tyrosine hydroxylase, clone LNC1, Millipore, Billerica, MA) in 2% NHS/TBST-0.2% ...
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bioRxiv - Neuroscience 2020Quote: ... followed by incubation at 4°C in a 1:500 dilution of the anti-Calbindin-D-28K antibody (Sigma, CB-955) in blocking solution for 48 hours ...
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bioRxiv - Neuroscience 2019Quote: ... for 1 hour and incubated overnight at 4°C with primary antibody (ATRX: 1:500, H-300 Santa Cruz Biotechnology Inc.; INCENP: 1:3000, Sigma). The following day the membrane was washed and incubated in the appropriate horseradish peroxidase-conjugated secondary antibody (1:4000 ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were applied overnight at 4 °C.” Sections were washed in 0.1M PBS and blocked with 5% Chemiblocker (Millipore, Darmstadt,Germany) comprising 0.5% Triton X-100 and 0.05% NaN3 in PBS at pH=7.4 ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were then incubated overnight at 4°C in PBST-NGS containing mouse monoclonal anti-α-Tubulin-FITC antibody (Sigma-Aldrich) at a dilution of 1:200 ...
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bioRxiv - Genetics 2021Quote: ... for 1 hour at 4°C and then incubated overnight on a rotor with 3 μL anti-FLAG antibody (Sigma, F3165). Each reaction was incubated with Protein-G agarose beads (50 μL ...
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bioRxiv - Genetics 2020Quote: ... Samples were immunoprecipitated with S9.6 antibodies (8 μ antibody/ 5μ DNA) overnight at 4°C followed by incubation for 3 hours with protein G-Sepharose beads (Millipore) and washed sequentially ...
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bioRxiv - Physiology 2021Quote: ... Sections were then incubated for 45 minutes at 37°C with the primary antibody at 1:100 for anti-laminin (L9393, Sigma-Aldrich). Three washes were then performed with PBS ...
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bioRxiv - Physiology 2021Quote: ... Membranes blocked with 5% non-fat milk in PBS-T or Tris-Buffered Saline TBS (TBS-T) (RT, 60min) and immunoblotted using the following primary antibodies (4°C, overnight): rabbit anti-pSmad1/5/8 (Merck Millipore, AB3848), rabbit anti-pSmad2 (Cell Signaling ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein samples were resolved using 10% SDS-PAGE and analyzed by Western blotting using anti-c-Myc antibody (Sigma-Aldrich, USA) and anti-PAX9 antibody (Cell signalling technology ...
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bioRxiv - Neuroscience 2021Quote: ... plus 0.1 % Triton X-100 and incubated overnight at 4 °C or for 2 h at room temperature with the following primary antibodies: anti-REST (1:500; 07-579 Millipore, MA), anti-Npas4 (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... pH 7.4) for 1 hour at room temperature and then incubated at 4°C overnight with primary antibodies (anti-multicillin, Sigma Aldrich, HPA058073 1:500 ...
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bioRxiv - Developmental Biology 2021Quote: ... then incubated with primary antibodies diluted in 5% milk overnight at 4°C (anti-ATG13 (SAB4200135, 1:100, Sigma, MI, USA); anti-GAPDH (10494-1-AP ...
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bioRxiv - Genomics 2021Quote: ... The membranes were incubated overnight at 4°C with a 1:5000 dilution of monoclonal rabbit anti-HA antibodies (Sigma-Aldrich) or 1:5000 dilution of monoclonal rabbit anti-FLAG antibodies (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2020Quote: ... Blocking with 5% BSA in PBS for 1 hour at 37°C and incubation with an anti single-stranded DNA antibody (Sigma, MAB3034) diluted 1:80 in 5% BSA in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... The sections from the animals of different treatment groups were washed using PBS and permeabilized with 0.3% Triton X-100 and followed by incubation overnight at 4°C with the following primary antibodies:: rabbit anti-NeuN (1:100, Sigma-Aldrich), mouse anti-GFAP (1:500 ...
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bioRxiv - Developmental Biology 2022Quote: ... for 1 h at RT and directly labelled overnight at 4°C with primary antibodies (1:400 mouse mAB for sarcomeric alpha-actinin [CloneEA-53, Sigma-Aldrich, A7811] and 1:200 rabbit pAB for cardiac Troponin T [Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated overnight at 4°C in anti-NeuN primary antibody (1:5000; monoclonal-Mouse Cat# MAB377; Millipore, California USA) in PBSTx with 1% normal goat serum (NGS ...
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bioRxiv - Cancer Biology 2022Quote: Cryopreserved bone marrow mononuclear cells were thawed and stained using standard procedures (30 min, 4°C) with the surface antibody CD34-PE (clone AC136, dilution 1:50, MACS) and DAPI (Sigma-Aldrich), according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Coverslips were then incubated for 1 h at 37 °C with secondary antibodies each harboring a PLA probe (Duolink In Situ PLA MINUS/PLUS probes, Sigma-Aldrich). The PLA probes that bind to the constant regions of the primary antibodies contain a unique DNA strand ...
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bioRxiv - Biophysics 2022Quote: ... the cells were incubated for 1 hour at 37°C with 1:300 mouse anti-α-tubulin antibody (Sigma Aldrich, T6199) in PBS + 1 % BSA ...
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bioRxiv - Biophysics 2022Quote: ... the cells were incubated for 1 hour at 37°C with 1:300 mouse anti-α-tubulin antibody (Sigma Aldrich, T6199) in PBS + 1 % BSA ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was then incubated with primary antibodies overnight at 4°C: anti-Alpha Tubulin (1:200, DSHB 12G10) and β-catenin (1:10,000 dilution, Sigma ABE208). The membrane was washed three times using PBST ...
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bioRxiv - Cell Biology 2022Quote: ... and then immunoblotted with the following primary antibodies in TBST with 5% milk at 4°C overnight: mouse anti-Miro1 (WH0055288M1, Sigma-Aldrich) at 1:1,000 ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were blocked with 5% BSA in Tween-TBS at room temperature for 2 h and incubated at 4 °C overnight with anti-His antibody (1:200; Sigma Aldrich) in blocking buffer ...
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bioRxiv - Neuroscience 2022Quote: ... The membranes were subjected to the following primary antibodies overnight at 4°C according to the experiment: mouse monoclonal anti-β-tubulin III (1:5000; Sigma) or mouse monoclonal anti-actin (1:4000 ...
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bioRxiv - Plant Biology 2022Quote: ... Slides were then washed five times with PBS-BSA at 25°C followed by incubation in a 1:200 dilution of the tetramethylrhodamine-isothiocyanate-labelled goat anti-rabbit antibody (TRITC, Sigma-Aldrich) and fluorescein isothiocyanate-labelled goat anti-mouse antibody (FITC ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were incubated with a mouse monoclonal antibody recognizing the C-terminal end of connexin 43 (Cx43) (Sigma Aldrich, Cat# MABT901) at a 1:100 dilution in 5% BSA-PBS-T overnight at 4°C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... for 3 hours and then incubated overnight at 4°C in block with primary antibodies: mouse anti-acetylated tubulin (Sigma, T6793) at 1:250 or rabbit anti-phosphorylated Smad1/5/8 (Cell Signaling ...
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bioRxiv - Molecular Biology 2023Quote: ... Sections were incubated with primary antibodies overnight in a humidified chamber at 4°C with α-actinin (1:1,000, Sigma, A7811). Slides were incubated with secondary antibody (Alexa Fluor Plus 488 ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 hour and incubated overnight at 4°C with primary antibodies to label cells containing tyrosine hydroxylase (anti-tyrosine hydroxylase monoclonal, 1:1000 Millipore, MAB318) and mCherry (anti-dsRed polyclonal ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated in anti-TH monoclonal rabbit IgG primary antibody (1:2000 overnight at 4°C; Sigma-Aldrich) in solution with PBS and normal goat serum (NGS) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Lysates were then immunoprecipitated overnight at 4°C with TrkA antibody (1:100, 06-574, Sigma-Aldrich, St. Louis, MO, USA) or p75NTR antibody (1:100 ...
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bioRxiv - Physiology 2023Quote: ... and then incubated overnight at 4°C in primary antibodies diluted in 50% blocking buffer / 50% Tris buffered saline (bioWORLD #42020056–3) + 0.1% TWEEN (Sigma-Aldrich #P9416) (TBS-T) ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were incubated for 48 h at 4°C with primary mouse anti-TH antibody (Cat#MAB-318, Millipore Sigma) at 1:1000 dilution ...
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bioRxiv - Microbiology 2023Quote: ... lysates corresponding to 1 OD600 of cells were analyzed by SDS-PAGE and immunoblotted with either anti-c-Myc (9E10, Covance Research) for Myc-tagged proteins or anti-peroxidase soluble complex antibody (Sigma, P2416) for TAP-tagged proteins ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked at room temperature for 1 hour in 1% casein/PBS and probed overnight at 4°C with a 1:1000 dilution of Roche monoclonal 3F10 rat anti-HA-tag antibody (Sigma 11867423001) and 1:1000 dilution of rabbit anti-P ...
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bioRxiv - Biochemistry 2023Quote: ... the cell lysate was incubated (4 °C for 2 h) with anti-V5 agarose affinity gel antibody produced in mouse (Cat# A7345 from Millipore Sigma). After three repetitive washes (10 min each time ...
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bioRxiv - Biochemistry 2023Quote: ... cells were incubated with secondary oligonucleotide-linked antibodies for 1 hr at 37°C (anti-mouse PLUS and anti-goat MINUS, Duolink, Sigma-Aldrich), and processed according to the Duolink PLA fluorescence protocol (Sigma) ...
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bioRxiv - Synthetic Biology 2023Quote: ... in TBS-T for 1 h at room temperature and stained overnight at 4 °C with a rabbit anti-FLAG antibody (1:1000 dilution, Millipore F7425). After removing unbound antibodies by three 5-minute washes with TBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... An additional washing step was followed by a 1 h incubation at RT with the primary mouse anti-c-myc antibody (500 µl, 1:200, Sigma Aldrich). Further ...